• 수산해양교육연구
• /
• 제27권5호
• /
• pp.1491-1498
• /
• 2015

In this study we investigated the effect of vaccine efficacy and immune activities by feeding additives several natural herbal remedies extracts of Sweet Wormwood (Artemisia annua), Galla Rhois, Oriental raisin tree (Havenia Dulicis) and 6-mixed herb to Oilve flounder, Paralichthys olivaceus. Each group of fish fed with the herbal remedies added feed and basic formula during the seven weeks and vaccinated with Edwardsiella tarda Formalin killed vaccine after 4 weeks feeding. Relative Percentage Survival values (RPS) in the group was assessed by the challenge with E.tarda. All groups with the herbal remedies added feed enhanced growth rate, but there were not significantly different in lysozyme activity and agglutination titer. In a challenge experiment with E.tarda, RPS in the all groups feeding additives natural herbal remedies was higher than that of the control group. These results suggest that the natural herbal remedies extracts of Sweet Wormwood (Artemisia annua), Galla Rhois, Oriental raisin tree (Havenia Dulicis) and 6-mixed herb would be effective to enhance efficacy of vaccination to Olive flounder.

• ALGAE
• /
• 제19권1호
• /
• pp.7-14
• /
• 2004

Blue-Min was initially developed as an adsorbent for harmful gas removal and recently improved to apply to livestock, agriculture and aquaculture as an assistant feed. In the Blue-Min treatment, growth of harmful algae (Cochlodinium polykrikoides and the others causing the red-tide in the ocean) were inhibited below 10% in comparison with control and coagulation removal of harmful alge with Blue-Min treatment was more efficient than that of yellow loess treatment. It would be expected that the Ble-Min can be useful for the extirpator against the red-tide organisms and restrain the toxic algal growth around the fish aquaculture using the assistant feed. Recently, its utility has become to be diverse as it was revealed that aquaculture productivity increase by its application and, in addition, that it improve the water quality or sediment conditions in the aquaculture of Chinese White Shrimp. When Blue-Min was treated with the proper dose, the growth inhibition of Microcystis aeruginosa and lsochrysis galbana, which are typical red-tide organisms in freshwaters and food organisms in aquaculture, respectively, were less than that of marine red-tide organisms, while their growth slightly increased with low concentration treatiment. In addition, polyunsaturated fatty acids (PUFA) content of I. galbana slightly increase with the Blue-Min treatment. Through our research, the Blue-Min has diverse and comples function against various biological organisms and is proved as a biological activator or depressor.

• Asian-Australasian Journal of Animal Sciences
• /
• 제11권1호
• /
• pp.71-77
• /
• 1998

The experiment was conducted to evaluate Bio-V-Pro as an alternative protein source in broiler diets. Treatments were control, Bio-V-Pro 1% (BP 1), Bio-V-Pro 3% (BP 3), and Bio-V-Pro 5% (BP 5). During the starter period, all treated groups showed beter performance than control group in weight gain and feed intake (p < 0.05). The best FCR was observed in BP 1 group and the worst was found in BP 5 group. For the overall period, chicks fed BP 1 and BP 3 diets gained more than the chicks fed the control and BP 5 diets. All Bio-V-Pro fed groups consumed more feed than the control group (p < 0.05). FCR were similar between the control and BP 1 group, but BP 3 and BP 5 groups showed higher FCR than the control group. During the starter period, all BP groups showed better utilizability of crude protein, crude ash and phosphorus than the control group (p < 0.05). For the finisher period, except for the BP 5 group, the nutrient utilizability was not significantly different in most nutrients. Nutrients utilizability had a tendency (not significant) to decrease as the level of Bio-V-Pro in the diet was increased. Lysine utilizability was not affected by the dietary treatment, however, methionine utilizability was higher in BP 1 and BP 3 group than in the control group. From the results of this experiment, it seems that Bio-V-Pro is a good alternative protein source which can replace fish meal in starter diet up to 3% of the diet effectively.

• 한국수산과학회지
• /
• 제8권3호
• /
• pp.181-184
• /
• 1975

체장 약 10cm 되는 잉어 치어를 이용하여 염도 $20\%_{\circ}$까지의 범위내에서 실험실내 수조에서 그들의 저항성과 성장에 관한 일련의 실험을 한 결과는 다음과 같았다. 염분도 $12\%_{\circ}$까지는 담수에서 바로 옮겨도 죽지 않았으며, 낮은 염분에서 적응시켜 높은 데로 옮기니 $15\%_{\circ}$까지 대부분 잘 살아 남았다. 실험기간의 수온은 실온하에서 $14\~27^{\circ}C$의 범위내였다. 성장실험에서는 $2\~8\%_{\circ}$ 범위서는 생사료인 실지렁이를 사용했을 때나, 건조 pellet 사료를 사용했을 때나 다 같이 성장률과 사료효율이 대단히 높았다. 그러나, $12\%_{\circ}$에서는 실지렁이를 주었을 때는 아주 좋지 못했지만, pellet에서는 성장율은 조금 낮았지만 사료효율은 대단히 높았다.

• 한국수산과학회지
• /
• 제48권4호
• /
• pp.454-458
• /
• 2015

This study compared the effects of dietary supplementation of citrus by-product (CBP) and CBP fermented with Bacillus subtilis (F-CBP) on growth performance, feed utilization, intestinal histology and innate immunity of red seabream Pagrus major with three commercial immune-boosting products. The six experimental diets were supplemented with L-ascorbyl-2-polyphosphate (LAPP; the control diet), CBP or F-CBP at a concentration of 100 mg vitamin C equivalent/kg diet or one of three commercial immune boosters. The Experimental diets were fed to triplicate groups of 17 fish (initial body weight, 116 g) for 8 weeks. The water temperature during the feeding trial was maintained at $13-15^{\circ}C$. Growth and feed utilization did not differ significantly among the six dietary treatments, nor did the phagocytic activity, superoxide dismutase or total immunoglobulin concentrations. However, myeloperoxidase activity was significantly higher in the CBP groups. For the intestinal histology, the intestine diameter, villi and enterocyte heights and number of goblet cells did not differ significantly among groups. Therefore, CBP or F-CBP can be used as a valuable eco-friendly byproduct in diets for fishes including red seabream to maintain their normal growth and health.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제15권2호
• /
• pp.157-160
• /
• 2007

Nymph of the emma field cricket, Teleogryllus emma, were reared on several types of artificial diets. The development period of nymphs were 55.4 days when only a single food, wheat bran, was provided, and it did not show a significant difference compared to the rearing results of the Danong diet and mixed diet. The supplying period of fish meal as the animal feed, the high emergence rates were obtained at 3rd instar with 90% and 4th instar with 100%. For the added amount test, when more than 40% of the diet was added, it confirmed that the insect weight increased. The characteristics of development according to each added amount of the vegetable food (dry bean-curd residue and corn powder) were investigated to minimize the dangers of the degeneration of diet when rearing with a single feed during the $1st{\sim}3rd$ instar period. First, as the added amount of bean-curd residue increased, nymphal development period became longer and the emergence rate became low. With corn powder as the single diet, all died before becoming adult. However, when corn powder was added up to 30%, no difference existed in the breeding results.

• 한국양식학회지
• /
• 제21권2호
• /
• pp.107-110
• /
• 2008

본 연구에서는 육성기 조피볼락 사육용으로 부상배합사료의 효과를 조사하기 위하여 평균 체중 112 g의 조피볼락을 8개월간 사육하였다. 실험사료로 4종류의 부상배합사료를 준비하여 1일 1회 만복으로 공급하였다. 사육실험 결과, 생존률은 모든 실험구에서 100%로 양호하였다. 성장률, 사료효율, 단백질효율, 사료섭취율 및 간중량지수 모두 각 실험구간에 유의한 차이가 없었다. 사육실험 종료시, 전어체와 간의 일반성분도 모든 실험구간에 차이가 없었다. 이상의 결과로 볼 때, 본 연구에 사용된 실험사료 조성은 육성기 조피볼락 양식을 위한 실용사료의 조성으로 사용하여도 좋을 것으로 판단된다.

• 한국수산과학회지
• /
• 제50권1호
• /
• pp.15-24
• /
• 2017

This study examined the effects of dietary supplementation with citrus by-product (CBP) on the growth, feed utilization, innate immunity, and histology of the gills and intestine of juvenile olive flounder Paralichthys olivaceus during low water temperature season. A vitamin C-free basal diet was regarded as a control and five other diets were formulated that contained 30 and 300 mg of L-ascorbyl-2-polyphosphate (LAPP) or CBP as vitamin C equivalents/kg diet, or the antibiotic oxytetracycline (OTC) (designated as control, LAPP30, LAPP300, CBP30, CBP300, and OTC, respectively). Olive flounder (initial body weight $44.6{\pm}0.32g$) were fed the six experimental diets to apparent satiation for 9 weeks. Growth and feed utilization were significantly higher in CBP30 than in the control and LAPP groups. The lysozyme activity was significantly higher in CBP30 than in LAPP300. Enterocyte height was significantly higher in CBP30 than in the control. The number of goblet cells was increased significantly with LAPP30, LAPP300, and CBP30. These results demonstrate that CBP can reduce or replace vitamin C and antibiotic in the diet of olive flounder during the low-water-temperature season. The optimal CBP supplementation level seems to be approximately 1%, which is equivalent to 30 ppm vitamin C/kg in the fish diet.

• Animal cells and systems
• /
• 제16권2호
• /
• pp.162-171
• /
• 2012

We investigated food webs of a $Salix$ $subfragilis$-dominated wetland in the Janghang wetland in the Hangang estuary, which is very close to the Demilitarized Zone, along the west coast of Korea. Our study focused on understanding sesarmine crab ($Sesarma$ $dehaani$)-related food webs in a $S.$ $subfragilis$ forest. For our study, we used carbon and nitrogen stable isotopes and fatty acid biomarkers. We collected samples of plants, animals, and detrital sediment from four quadrats ($5{\times}5m^2$) set in the $S.$ $subfragilis$ community. Samples were collected from September 2006 to June 2009, except during the winter hibernation period of $S.$ $dehaani$. In the wet season, the sediment showed relatively high ${\delta}^{13}C$ and low ${\delta}^{15}N$ signatures compared with relatively low ${\delta}13C$ and high ${\delta}15N$ signatures in the dry season. Mature $S.$ $dehaani$ appeared to feed on fresh leaves and other carbon sources, such as immature individuals or fish, in addition to detrital sediment, which appeared to be the main carbon source for immature crabs. Principal component analysis of fatty acid biomarkers of $S.$ $dehaani$ showed a clear difference between immature individuals (10-30 mm) and mature ones (larger than 30 mm), indicating that the main food source for immature crabs was detrital sediment, whereas mature crabs foraged plants in addition to consuming detrital sediment. On the basis of our results from stable isotope and fatty acid analyses, mature $S.$ $dehaani$ appeared to feed on detrital sediment and fresh leaves of $S.$ $subfragilis$ in summer in addition to engaging in cannibalism of immature individuals.

• Asian-Australasian Journal of Animal Sciences
• /
• 제32권5호
• /
• pp.675-690
• /
• 2019

Objective: This study investigated the effect of diet and roughage quality (RQ) on dry matter intake, duration and number of daytime and night-time eating bouts, idling sessions and ruminating activities in small ruminants. Methods: In Exp 1 and 2, RQ was improved by urea treatment of veld hay, while diet quality was improved by supplementing with Lucerne hay (Exp 3), sunflower meal and lespedeza (Exp 4), fish meal (Exp 5a), and sunflower meal (Exp 5b). In all experiments goats and sheep were blocked by weight and randomly allocated to experimental diets. Day-time (06:00 to 18:00 h) and night time (18:00 to 06:00 h) feeding behaviour activities were recorded. Results: RQ affected rumination index in Exp 1, but not in Exp 2, 3, and 5. Time spent eating and ruminating was affected by RQ (Exp 1, 3, and 4), period of day (all experiments) and their interaction (Exp 1). Intake rates (g/bout and g/min) were similar across diets. Period of day affected the duration of rumination sessions (Exp 1, 2, and 3); diet or RQ affected the duration of eating bouts (Exp 3) and rumination sessions (Exp 1 and 2). RQ had a significant effect on the duration of eating sessions in Exp 3 only, whilst period of day affected this same behaviour in Exp 2 and 3. Generally, goats and sheep fed on roughage alone ruminate at night and eat more during the day but those fed a roughage and supplemented with Lucerne hay spent more time ruminating than eating. Time spent eating and ruminating had positive correlations to crude protein and feed intake. Intake rates had strong positive correlations to intake. Conclusion: Chewing time, number of eating and ruminating sessions, and duration of eating bouts are physiologically controlled in small ruminants, though chewing time requires isometric scaling during modelling of intake.