• Fisheries and Aquatic Sciences
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• v.22 no.4
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• pp.8.1-8.9
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• 2019

A 12-week feeding trial was designed to evaluate the effect of total replacement of fish oil (FO) with terrestrial alternative oils on growth, feed utilization, body composition, hematological parameters, and fillet fatty acid profile of mandarin fish juveniles. Four iso-nitrogenous (56% crude protein) and iso-lipidic (13% crude lipid) practical diets were formulated. A control diet contained 6% FO and three other experimental diets were prepared by replacing FO with linseed oil, soybean oil, and lard (designed as FO, LO, SO, and lard, respectively). Each diet was randomly allocated to triplicate groups of 25 fish ($1.8{\pm}0.03g/fish$) in a circular tank. Complete replacement of FO by three tested alternative oils had no remarkable impact on growth performance, feed utilization efficiency, and morphological and hematological parameters of juvenile mandarin fish. However, daily feed intake was found to be significantly higher for fish fed the SO diet compared with those fed the FO and LO diets. Fish fed LO and SO diets exhibited significantly higher levels of the whole body lipid compared to fish fed diet containing FO. Fillet fatty acid composition reflected dietary fatty acid profile. The highest level of ${\alpha}$-linolenic acid, linoleic acid, and oleic acid was observed in fish fillet fed LO, SO, and lard, respectively. Although the eicosapentaenoic acid level of fish fillet fed diet FO was higher than other treatments, no significant difference was found in docosahexaenoic acid content among all dietary groups. The results of the present study clearly demonstrate that the complete replacement of FO in mandarin fish diets is achievable. These findings are useful in dietary formulation to reduce feed costs without compromising mandarin fish growth.

• Fisheries and Aquatic Sciences
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• v.26 no.10
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• pp.583-592
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• 2023

Celebes rainbow (Marosantherina ladigesi) is one of Indonesia's exported ornamental fish commodities, but the exploitation of this fish only relies on wild catches. The rise of unlimited fishing, especially those using poison, has changed the aquatic environment, threatening sustainability and causing fish extinction. This study aimed to evaluate the effectiveness of several types of feed in improving the absolute growth rate (AGR), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), feed efficiency (FE), hematology, and immune response of Celebes rainbow. The fish used in this study were male ornamental Celebes rainbow (M. ladigesi) weighing 1.32 ± 0.21 g/ind, reared in 54 L-aquariums at a stocking density of 30 individuals/aquarium for six weeks. The fish were fed according to the test diet consisting of live Tubifex sp worms, dry Tubifex sp worms, Spirulina platensis, and crumble pellets. The parameters observed were AGR, SGR, SR, FCR, FE, hematology, intestinal histology, liver histology, and a challenge test with the pathogenic bacteria Aeromonas hydrophila. The results showed that fish-fed live Tubifex sp worms had better AGR, SGR, SR, FCR, FE, hematology, and disease resistance compared to all other treatments. These results indicate that live Tubifex sp worms are the best feed for rearing Celebes rainbow.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.720-726
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• 2006

In an attempt to develop an artificial diet for growing olive flounder (Paralichthys olivaceus), weight gain, feed utilization and nutrient retention were investigated in fish fed moist (MP), semi-moist (SMP) and extruded pellets (EP). Excretion of nitrogen and phosphorus was also estimated based on their whole body gain and intake. EP and MP composed of raw fish and SMP made of formulated powder feed with water were prepared to have the same energy contents on a dry matter basis. A total of 240 fish with an average initial weight of 120 g were randomly distributed to each (20 fish/tank) of 12 circular plastic tanks (4 tanks/treatment) and fed experimental diets for 8 weeks. Fish groups fed EP (209 g) or SMP (209 g) recorded higher final weight than those fed MP (176 g), while dry feed consumption was highest in SMP groups (106 g), followed by MP (71 g) and EP groups (62 g). As a consequence, fish groups fed EP showed much lower feed conversion ratio than the other two groups. Protein efficiency ratio was also significantly higher in fish groups fed EP (2.55) than in those fed MP (1.44) and SMP (1.31). Fish groups fed EP, which showed the highest nitrogen retention of 43.9%, resulted in the lowest excretion of nitrogen of 35.5 g per kg gain. Also, the lowest phosphorus excretion of 6.0 g per kg gain was found in the EP groups with the highest P retention (37.0%) among treatments. Although the EP groups had the lowest dietary energy intake, they retained the highest energy in the whole body among treatments. The present results showed that EP could be more advantageous than MP or SMP in terms of growth, feed utilization and excretion of nitrogen and phosphorus for olive flounder.

• Fisheries and Aquatic Sciences
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• v.5 no.3
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• pp.229-234
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• 2002

Possibility of raising sunshine bass during the winter season in Korea was investigated. Also, the effect of feed on growth and body composition of sunshine bass was compared at the various salinity. Twenty sunshine bass $(Mean\;weight\;\pm S.D.\: :\;53.9 \pm 0.24 g)$ were stocked into the eighteen circular flow-through tanks. A 2 (feed) $\times$ 3 (salinity) factorial design with triplicate was used for this study. Feed was prepared into the 2 groups: the raw fish-based me>ist pellet (MP) containing $59.5\%$ crude protein and $9.4\%$ crude lipid, and commercial sinking red sea bream feed (CF) containing $49.9\%$ crude protein and $9.4\%$ crude lipid, respectively. And salinity was prepared into the 3 groups: freshwater $(0\%)$, brackishwater $(15\%)$, and seawater $(32\%)$. Fish were fed to satiation twice daily for 6 days a week. Fish were all survived at the end of the 8-week feeding trial. Weight gain (WG) and specific growth rate (SGR) of sunshine bass were significantly (P<0.05) affected by both feed and salinity. WG and SGR of sunshine bass fed the MP were significantly (P<0.05) higher than those of fish fed the CF in the same salinity. Amount of dry feed fed was significantly (P<0.05) affected by both feed and salinity. Feed consumption by sunshine bass fed on the MP was significantly (P<0,05) higher than by fish fed on the CF in the same salinity. Feed efficiency ratio (FER) was significantly (P<0,05) affected by salinity, but not by feed. Protein efficiency ratio (PER) was significantly (P<0.05) affected by both feed and salinity. Moisture and protein content of the whole-body of fish was not significantly (P>0.05) affected by either feed or salinity. However, lipid and ash content of the whole-body of fish was significantly (P<0.05) affected by salinity, but not by feed. In conclusion, the MP was superior to CF for growth of sunshine bass during the winter season in Korea and no mortality occurred. And sunshine bass seemed to grow better in freshwater $(0\%)$ and brac-kishwater $(15\%)$ than seawater $(32\%)$ under these experimental conditions, in terms of improvement in FER and PER in freshwater or brackishwater.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.3
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• pp.394-399
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• 2011

The effect of intermittent feeding on growth, feed utilization and body composition of subadult olive flounder fed extruded pellet (EP) in suboptimal temperature ($13.5{\pm}2.10^{\circ}C$) was determined. Two hundred twenty five subadult fish averaging 272 g were randomly distributed into fifteen of 300 L circular flow-through tanks (fifteen fish per tank). Five treatments with different days of feeding a week were prepared in triplicate: fish were hand-fed with EP to apparent satiation once a day, seven days a week (7DF), which was used as the control group or consecutive six, five, four and three days a week, for 12 weeks, referred to as 6DF, 5DF, 4DF and 3DF treatments, respectively. Weight gains and specific growth rates of fish in 6DF treatment were higher than those of fish in 7DF, 4DF and 3DF treatments, but not different from those of fish in 5DF treatment. Daily feed intake of fish in 6DF and 7DF treatments was higher than that of fish in 5DF, 4DF and 3DF treatments. Feed efficiency and protein efficiency ratio of fish were not different among treatments. There was no difference in the chemical composition of fish between treatments. Results of this study demonstrated that feeding five days per week could be recommended for subadult olive flounder fed extruded pellet in suboptimal temperature.

• Fisheries and Aquatic Sciences
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• v.16 no.1
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• pp.57-61
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• 2013

I hypothesized that the manipulation of dietary nutrient contents including phosphorous could affect compensatory growth of juvenile olive flounder, Paralichthys olivaceus. Thirty fish averaging 34.8 g per tank were randomly chosen and distributed into 15 flow-through 180-L tanks. Three experimental diets were prepared: the control (C) diet, high protein and lipid (HPL) diet, and HPL diet with supplementation of calcium phosphate-monobasic (HPLP). Five treatments were prepared in triplicate: fish were hand-fed daily with the C diet twice a day for 8 weeks (C-8W); fish were starved for 1 week, and then fed with the HPL or HPLP diets twice a day for 7 weeks, and referred to as HPL-7W and HPLP-7W, respectively; and fish were starved for 2 weeks, and then fed with the HPL or HPLP diets twice a day for 6 weeks, and referred to as HPL-6W and HPLP-6W, respectively. The body weight of fish with C-8W, HPL-7W and HPLP-7W treatments was higher than fish with HPL-6W and HPLP-6W treatments on week 2, 4 and 6 after an initiation of the trial. At the end of the 8-week trial, fish with HPLP-7W and HPL-7W treatments overcompensated, as compared to fish with C-8W treatment. Full compensation was not achieved in fish subjected to the 2-week feed deprivation (HPL-6W and HPLP-6W treatments). Overall feed intake by fish was proportional to weeks of feeding. Feed conversion ratio of fish with HPLP-7W, HPL-6W and HPLP-6W treatments was higher than fish with C-8W treatment. The study showed that dietary supplementation of protein and lipid resulted in overcompensation of juvenile olive flounder subjected to a 1-week feed deprivation, but not a 2-week feed deprivation. Additionally, dietary supplementation of phosphorous did not further improve compensatory growth of fish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.756-761
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• 2017

This study investigated the growth of juvenile mandarin fish Siniperca scherzeri, reared at high water temperatures. Feed-trained juvenile fish were reared at four water temperatures: 27, 29, 31, and $33^{\circ}C$. The fish were fed to apparent satiation twice daily using pelleted diet. After 10 weeks of feeding trial, the survival exceeded 98% in all groups. The weight gain of fish reared at $31^{\circ}C$ was higher than for fish reared at the other temperatures. The feed efficiency and protein efficiency ratio of fish reared at 29 and $31^{\circ}C$ were higher than for the fish reared at 27 and $33^{\circ}C$. The daily feed intake of fish reared at 27 and $33^{\circ}C$ was higher than for the 29 and $31^{\circ}C$ groups. The whole body moisture content of fish reared at $33^{\circ}C$ was higher than in the $27^{\circ}C$ group. The whole body crude protein and lipid contents of fish reared at $27^{\circ}C$ were higher than in the $33^{\circ}C$ group. The results indicate that suitable rearing water temperature was $31^{\circ}C$ for optimal growth and feed efficiency of juvenile mandarin fish under the experimental conditions.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.6
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• pp.762-769
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• 2017

This study investigated the effects of stocking density on the growth of the mandarin fish Siniperca scherzeri, in small (1-year old, 50.2 g/fish) and large (2-years old, 173.1 g/fish) size (age) groups. Small and large fish groups were fed pelleted diets twice daily for 10 and 15 weeks, respectively. In the small group, at the end of the feeding, the weight gain of fish at a density of $4kg/m^3$ was lower than that of fish at densities of 3, 2 and $1kg/m^3$. However, fish stocking density did not affect feed efficiency. The daily feed intake of fish at a density of $4kg/m^3$ was lower than that fish at densities of 3, 2 and $1kg/m^3$. In the large fish group, the weight gain of fish at a density of $4.5kg/m^3$ was higher than that of fish at densities of 6, 3 and $1.5kg/m^3$. Again, fish stocking density did not affect feed efficiency. The daily feed intake of fish at a density of $4.5kg/m^3$ was higher than that of fish at densities of 6, 3 and $1.5kg/m^3$. Based on these results, the optimum stocking density for small (50.2 g/fish) and large (173.1 g/fish) mandarin fish is 3 and $4.5kg/m^3$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.5
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• pp.683-688
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• 2016

The effects of dietary supplementing natural additives [yacon (YC), ginger (GG), and blueberry (BB)] on growth, feed utilization, and body composition of juvenile rockfish were compared to those of ethoxyquin (EQ), which is commonly used to prevent rancidity of fish feed. A total of 480 fish were distributed randomly into 12 50-L flow-through tanks (40 fish/tank). Four experimental diets were prepared: the control diet (Con) with 0.01% EQ, and the YC, GG and BB diets containing 1% YC, GG, and BB instead of wheat flour. Each diet was assigned randomly to triplicate tanks of fish, which were hand fed to satiation twice daily for 8 weeks. Weight gain of fish fed the YC diet was higher than that of fish fed all other diets. Feed efficiency (FE), protein efficiency ratio (PER), and protein retention (PR) in fish fed the YC diet were higher than those in fish fed the other diets. Whole-body chemical composition of the fish was affected by the additive sources. In conclusion, dietary supplementation with GG effectively improved weight gain and feed utilization (FE, PER, and PR) of juvenile rockfish.

• Fisheries and Aquatic Sciences
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• v.24 no.9
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• pp.311-317
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• 2021

Inclusion effect of various levels of jack mackerel meal (JMM) acting as feed attractants and/or stimulants in diets on the growth, feed consumption, feed utilization and whole body composition of rockfish was investigated. Three hundred juvenile rockfish were randomly allocated into 15 flow-through tanks. Five experimental diets supplemented with JMM at 0%, 1%, 3%, 5%, and 10% at the expense of anchovy meal were prepared and referred to as the JMM0, JMM1, JMM3, JMM5, and JMM10 diets, respectively. Weight gain, specific growth rate (SGR) and feed consumption of fish tended to improve with dietary levels of JMM. The greatest weight gain, SGR and feed consumption were observed in fish fed the JMM10 diet, followed by the JMM5, JMM3, JMM1, and JMM0 diets, in that order. Condition factor (CF) of fish tended to improve with dietary inclusion levels of JMM. Growth performance, feed consumption and CF of fish tended to improve with dietary inclusion levels of JMM ranging from 0% to 10%.