• 한국어병학회지
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• 제24권2호
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• pp.65-73
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• 2011

패류 내에 오염되어 있는 바이러스의 검출에 있어서 정성 정량적 분석이 기능하며 신속하고 간편한 방법의 개발을 이루고자 하였다. 5g의 굴 중장선 조직을 Glycine buffer 및 PEG 8000 용액을 사용하여 제조한 농축 시료 (T5g-D)와 50mg의 굴 중장선 조직으로부터 직접적으로 제조한 시료(sT5Omg-D)를 대상으로 2-step PCR을 실시하여 검출감도를 비교하였다. 동일한 1 ${\mu}l$의 DNA template T5g-D와 sT50mg-D를 사용하였을 때, 35cycles의 1-step PCR에서 양성의 결과를 얻을 수 있었다. 인위적으로 sT50mg-D 혼합 시료를 사용하여 2-step PCR (35cycles)을 실시하였을 때 T5g-D를 사용한 것과 비교하여 뚜렷한 차이를 나타내지 않았다. 또한 megalocytivirus에 오염된 양성시료 0.5~50mg을 사용하여 조제한 각각 $50{\mu}l$의 template DNA 1 ${\mu}l$를 사용하여 qPCR을 하였을 때 6.14E+00~1.2E+02/${\mu}l$의 농도를 함유하고 있는 것으로 나타났다. 조제한 template DNA에 6.14E+00 copies/${\mu}l$ 이하의 농도가 있을 때는 qPCR에서 positive의 결과를 얻을 수 없었다. 결론적으로 패류 내 mega1ocytivirus의 오염 유무 판단을 위한 2-step PCR 및 qPCR에서 50mg의 중장선을 사용하는 것은 i) 굴의 조직으로부터 오염된 megalocytivirus의 정성 및 정량을 위한 최소 검출 한계에 벼하여 충분한 양이었으며 ii) 개체별 분석이 가능하다는 장점이 있었으며 iii) 이를 토대로 국내에서 서식하고 있는 굴에서 megalocytivirus의 오염을 확인할 수 있었다.

• 수산경영론집
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• 제26권2호
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• pp.53-74
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• 1995

The purpose of this paper is to compare China's and Korea's marine fisheries industries in order to present the main features of China's marine fisheries and show the comparative advantages they have in production. The results indicate that China's marine fisheries have the following features. (1) The marine proportion of the fisheries industry economic activity is less than 60%. Fishing is 71.3% of that marine activity. (2) The production trends of marine fisheries history in China can be classified as follows: 1) an early growth period, 2) a deliberation/consolidation period, 3) a second growth period, 4) a third growth period, and 5) a fourth growth period. The growth rate has rapidly increased recently. (3) Fish production is over 70% of marine fishing fisheries, the next major product is crustacea. The production of shellfish occupies over 70% of marine aquaculture, seaweed production however, is only 22% of total marine aquaculture. (4) The licensed area for marine aquaculture in China is 586.3 ha and that area is 5.4 times larger than that of Korea. The allotted area for shellfish aquaculture is 60% of marine aquaculture, production areas of crustaceans occupy 27.3%, fish has 7 1%, and seaweed production only 5.7% of allocated marine aquaculture areas. (5) The proportion of power vessels for marine fisheries of China's total power vessel fleet is around 65%, and the marine fisheries portion of non - powered vessels constitutes only 12%. The highest proportion of power vessels engaged in marine fisheries activities is between 10 tonnes to 100 tonnes. (6) The portion of marine fishery workers of all fishery industry employees is 22%, and 70% of them are full - time workers. Of marine fishery workers, 64% are in the fishing sector, 22%, aquaculture workers, and the number of employees in marine fisheries is increasing every year. The analysis of China's fishery industry in the production competitiveness indicates as follows : (1) The licensed areas in marine aquaculture, number of fishing vessels, number of marine fishing workers in China's fishery industry are much more than those of Korea's. Therefore China is much more competitive than Korea in the quantity of production side. However, licensed areas for seaweed aquaculture are more extensive in Korea than China. In China, the number of power vessels of between 10 tonnes and 100 tonnes, the licensed shellfish aquaculture areas, and the number of fishing workers within the fisheries industry are much more than those of Korea. (2) It is estimated that the licensed areas in marine aquaculture, number of medium sized power vessels, number of marine fishery workers will be increased as the quantity of production factors grow in China. (3) At present, yield per Ha. in marine cultures is very low in China. Therefore it is estimated that aquaculture techniques have only been diffused recently in China. Yield of fish per Ha especially is much lower than that of Korea. So the level of aquaculture techniques seems much lower than that of Korea. (4) China is behind Korea in production technique, however the number of HP per boat in China is lower than that of Korea. Therefore, China is much more competitive than Korea in Costs. (5) Average fish catches per marine fishery worker in China is only 1/3 that of Korea's, and average marine aquaculture production in China is only 1/2 that of Korea. Therefore we can say Korea is more competitive than China in efficiency. The average income of marine fishery workers in China is higher that that of other Chinese industries. However, the competitiveness of the fisheries industry in China will be increased as more capital is invested and advanced techniques are developed.

• Journal of Nutrition and Health
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• 제30권9호
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• pp.1088-1094
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• 1997

One hundred twenty healthy college students and housewives from urban middle income household were investigated in terms of food consumption patterns, nutrient intake and dietary fiber intake. Total food intake was slightly lower in college students(1059.9g/d) than in housewives(1160.g/d) . However, the ration of animal food to total food was considerably higher in college students(23.7%) than in housewives(16.8%). College students tended to consume more dairly products, meat & products, and cereals & grain products but less vegetables , fruits, fish and shellfish than housewives. Although nutrient intake was not much different (except for total calories, fats, and ascorbic acid), dietary fiber intake was slightly and significantly lower in college students(14.9g/d, 8.3g/1000kcal) than in housewives(16.9g/d, 103g/1000kcal) . That is partially due to college students irregular food habits and the change in their meal patterns from traditional to western diets. There was a significantly positive correlation between dietary fiber intake and thiamin, riboflavin, niacin and crude fiber intake.

• Journal of Nutrition and Health
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• 제16권1호
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• pp.34-40
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• 1983

Clam, Tapes japonica, has been widely used and occupies an important position in the markets. More than thirty kinds of fermented fish and shellfish are presently available in the markets in korea. This study was attempted to establish the basic data for evaluating the taste compounds of the clam. The free amino acids as taste compounds during the fermentation of the clam, Tapes japonica, were analyzed by an amino acid autoanalyzer. Alanine (17.0%), glycine (15.5%), taurine (13.9%) were high in amount in the fresh clam and then glutamic acid, arginine, tyrosine, asparagine, lysine, threonine, serine and leucine were the next in order. Such amino acids as methionine, phenylalanine, histidine and cystine were low in amounts. The total free amino acid nitrogen in the fresh clam was 45.5% of its extract nitrogen. During the fermentation of the clam, the kinds of the free amino acids were the same with the fresh samples although the amounts were changed. It is believed that glutamic acid, aspartic acid, glycine, alanine, lysine, and leucine may play an important role as the taste compounds in the fermented clam.

• 한국환경보건학회지
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• 제31권6호
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• pp.451-456
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• 2005

Minamata disease made its first appearance in the world at Minamata City, Kumamoto Prefecture, in May 1956. In 1962 methyl mercury poisoning through the placenta was found for the first time in the world. This was called congenital Minamata disease. In all cases the clinical symptoms were consistent with those of cerebral palsy. The time and place of outbreak were the same as those for Minamata disease. Their mothers had eaten fish and shellfish during pregnancy. The principal symptoms of congenital Minamata disease are mentalretardation ($100\%$); primitive reflexes ($100\%$); disturbance of coordination ($100\%$); dysarthria ($100\%$); limb deformation (100%); growth disorders ($100\%$); nutritional disorders ($100\%$); chorea-athetose ($95\%$); and hypersalivation ($95\%$). However, today, when the world is polluted by mercury in various places and at various levels, the data we need is not represented by those severe cases, but rather by the chronic milder type. Even in Minamata, the issue of Minamata disease has not been resolved. And likewise, on a global scale the problem of Minamata disease is not yet over.

• 한국어병학회지
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• 제22권3호
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• pp.229-237
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• 2009

The causative agent for the tunic softness syndrome of the cultured ascidian Halocynthia roretzi from Jan 1999 to Feb 2009 was identified using virus isolation and polymerase chain reaction (PCR). The pathogenicity of the isolated virus MABV UR-1 strain was determined by experimental infection trials. The cytopathic effects was observed in CHSE-214 cell line at a level 5.1% (4/78) in normal ascidian and 1.8% in abnormal ascidian showing tunic softness syndrome signs. MABV gene was detected in 16.8% (18/107) of normal and 13.1% (5/38) of abnormal organisms by PCR. The ratio of MABV isolation and gene detection was similar level in normal and soft tunic diseased ascidian. Based on the VP2/NS junction region sequences, eight strains of virus isolated from ascidian, were included in the same genogroup with MABV which is originally isolated in wide ranges of marine fish and shellfish species. The UR-1 strain caused 60% mortality (36.5% mortality in control group) by immersion infection and 37% mortality (same mortality in control group) in injection infection indicating no significant differences in infected and control groups. These results suggest that ascidian can act as reservoir of the MABV, and this virus is not directly related with the ascidian mortality.

• Ocean and Polar Research
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• 제31권2호
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• pp.157-165
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• 2009

The study examined overuse of the fishery resource. Influence of fishing activity was estimated by application of Schaefer model's. Fishing efforts that produced the maximum sustainable yield were determined in the model, allowing the effect of overfishing to be assessed. In the model, a wide variety of fish species as well as crustaceans and shellfish were susceptible to overfishing, while mollusks were not. Overfishing by modern techniques exacted a greater toll than more traditional methods. The results of the modeling study suggest that the 'Buy bag' input-control system of fisheries resource management warrants consideration, as does modernization, expansion and strengthening of self-control management of the fishery resource. Finally, more effective efforts in dissemination of policy information and education concerning the fishery resource are needed.

• 한국환경보건학회지
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• 제47권1호
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• pp.87-100
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• 2021

Objectives: The purpose of this study was to analyze the factors affecting Bisphenol A (BPA) exposure in children and adolescents using the results of the Korean National Environmental Health Survey (KoNEHS) cycle 3. Methods: A total of 2,380 subjects (n=571, 887, and 922 for 3-5, 6-11, and 12-17 years of age, respectively) were analyzed using an environmental exposure survey and environmental chemical substances concentration levels. Univariable linear regression analysis was performed to determine associated variables such as sex, age, income level, housing type, secondhand smoke time, cup noodles and canned food consumption, seafood consumption, new furniture (within the previous six months), drinking water type, and consumption of herbal medicines. Variables with p-values of less than 0.2 were extracted from the results and a multivariable linear regression analysis was performed using stepwise selection. Results: Univariable linear regression analysis showed positive associations between BPA concentration levels and variables including sex, age, secondhand smoke time, new furniture (within the previous six months), renovated living space (within the previous six months), fish and shellfish consumption, plastic-bottled drink consumption, and herbal medicine. As a result of performing multivariable linear regression analysis, the lower was the age the higher was the concentration of BPA levels. Additionally, women showed higher BPA levels than those of men. The more frequently fish was consumed, the higher was the BPA concentration. Moreover, higher BPA concentrations were observed when taking herbal medicine. Conclusions: The main factors affecting BPA concentration levels were age, gender, and consumption of fish and herbal medicine.

• 한국어병학회지
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• 제22권3호
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• pp.211-218
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• 2009

경북 울진 지역에서 채집된 양식 강도다리와 충남 태안 및 부산 지역 넙치 시료로부터 분리한 MABV에 대한 유전자 비교를 위해 VP2-NSPhylogenetic VP3 region (432 bp)을 phylogenetic 분석에 이용했다. Sequence 확인 결과 MABV (08-KU)는 일본 방어에서 최초 분리 보고된 YTAV와 98%의 nucleotide 유사성이 나타났으며, 이전 보고된 다 른 여러 strain들과는 76%이상 유사한 것으로 확인되었다. 그리고 MABV (06-KP)와 MABV (08-KC)도 YTAV와 97-98%의 높은 sequence 유사성을 보였다. 또한 다양한 MABV strain들과의 비교를 위해 충남태안 및 부산지역 넙치 시료에서 분리한 MABV (08-KC)와 MABV (06-KP)에 대한 phylogenetic 분석도 실시하였다. 그 결과 분석에 사용된 MABV (08-KU0, MABV (06-KP), MABV (08-KC)는 모두 일본 방어에서 분리된 MABV Y6와 동일한 genogroup VII에 포함 되었다.

• 한국지역지리학회지
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• 제12권3호
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• pp.339-351
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• 2006

뻘중심의 갯벌이 넓게 형성된 가로림만의 웅도리외 모래 갯벌과 염전이 발달한 영광 갯벌의 두우리, 수산 양식업이 발달한 곰소만 주변 지역을 대상으로 조석 주기, 갯벌 지형. 갯벌 퇴적물의 입도 특성과 주민 생활과의 관련성을 검토했다. 갯벌 주변 주민들에게 갯일은 농사일보다 우선되고, 갯벌에서 얻어지는 수입이 이들의 주소득원이다. 갯벌주변의 주민 생활은 조석 주기에 맞추어 이루어지고 있으며, 한사리 저조위 때는 가장 바쁘고 많은 소득을 올릴 수 있어 마치 장날과 같다. 뻘 갯벌에서는 패류 양식이 주이고, 모래 갯벌에는 안강망 어업이 활발하며, 해안에 접한 혼합 갯벌에는 염전이 발달한다. 또한 하나의 갯벌에도 경제 활동의 분화가 이루어져 조상대에는 염전과 양어장으로, 조간대에는 패류 양식장으로, 조하대는 굴, 김 양식징으로 이용된다.