• Title/Summary/Keyword: fingerprinting

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RAPD Fingerprinting for the Species Identification of Animals

  • Huang, Mu-Chiou;Horng, Yan-Ming;Huang, Hsiu-Lin;Sin, Yen-Long;Chen, Ming-Jaw
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.10
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    • pp.1406-1410
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    • 2003
  • The studies were based on the RAPD fingerprinting for the species identification of animals. The genomic DNA samples of ostriches, Taiwan local chickens, Aboracres broilers, Leghorn chickens, quails, doves, emus, Beltville small white turkeys, pheasants, Chinese geese, mule ducks, Holstein cattle and Landrace pigs were amplified with random primers by RAPD-PCR for fingerprinting. The results showed that the varied band patterns of DNA fingerprints were generated from templates depending on the kinds of primers or animal species. The same primer applied to the same breed, all of the main bands are similar, but which were different among species. In order to try to identify the species from the mixture of meat by RAPD fingerprinting, the meat of ostrich and cattle was mixed in different ratios for this study. The results showed that it could be easily and precisely distinguished according to the band distribution of RAPD patterns.

Genomic Fingerprinting of Staphylococcus aureus Isolated from Bovine Mastitis Milk by PCR (젖소 유방염 유래 Staphylococcus aureus의 PCR을 이용한 Genomic Fingerprinting)

  • Kim, Doo;Kwon, Soon-Tak;An, So-Jeo
    • Journal of Veterinary Clinics
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    • v.16 no.1
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    • pp.19-25
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    • 1999
  • A total of 137 strains of Staphylococcus aureus were isolated from dairy cow's milk with subclinical mastitis from 33 herds in 5 provinces and 36 strains of S aureus from clinical mastitis from 4 herds where the mastitis were severe problem. Arbitrary primed polymerase chain reactions with 10 bp oligonucleotide primer were performed and the PCR products were analysed with image analyzer, The S aureus strains were genotyped into 20 distinct DNA fingerprinting profiles. The size of PCR products ranged from 163 to 2,479 bp and PCR products of 506, 770, 784 and 2,479 bp were the most prevailing bands. Genotype 3 was founded in all 5 provinces. The various genotypes were identified in newly founded dairy herds, however, only one or two genotypes were identified in the closed herds. In clinical mastitis, only a limited number of different S aureus genotype was founded in each of the herds in comparision with subclinical mastitis. The results demonstrated that PCR-based DNA fingerprinting analysis of S aureus strain can be used to study epidemiology of mastitis, in addition, common genotype in geographic region can be useful for the development of an effective S aureus bacterin.

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Blind Video Fingerprinting Using Temporal Wavelet Transform (시간축 웨이블릿 변환을 이용한 블라인드 비디오 핑거프린팅)

  • Kang Hyun-Ho;Park Ji-Hwan;Lee Hye-Joo;Hong Jin-Woo
    • Journal of Korea Multimedia Society
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    • v.7 no.9
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    • pp.1263-1272
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    • 2004
  • In this paper, we present a novel video fingerprinting implementation method to identify the source of illegal copies. The video fingerprinting is achieved by the insertion of uniform distributed random number is made by seller and buyer's identification key-in the video wavelet coefficients by their temporal wavelet transform. The proposed fingerprinting is able to detect unique fingerprint of video contents even if they have been distorted by collusion attacks and MPEG2 compression. Especially, we use characteristics of the temporal wavelet transform to assign user's embedding area. Experimental results show the traceability of unauthorized distribution of video contents and its robustness to various collusion attacks and MPEG2 compression.

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Genomic Fingerprinting Patterns of Bifidobacteria Isolated from Healthy Koreans Using ERIC-, TAP-, and BOX-PCR (건강한 한국인으로부터 분리된 비피도박테리아의 ERIC-, TAP-, BOX- 중합효소연쇄반응을 이용한 유전자 지문 분석)

  • Lee, Do-Kyung;Kang, Byung-Yong;Chung, Myung-Jun;Lee, Kang-Oh;Kim, Kyung-Jae;Ha, Nam-Joo
    • Environmental Analysis Health and Toxicology
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    • v.23 no.1
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    • pp.1-9
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    • 2008
  • 유산균인 비피도박테리아는 사람과 동물에서 유익한 프로바이오틱 미생물로 알려져 있다. 본 연구에서는 이러한 비피도박테리아 균주의 분류를 위한 repetitive DNA element PCR fingerprinting (ERIC-또는 TAP-PCR)의 사용을 평가하였다. 사람분변으로부터 분리한 알려지지 않은 비피도박테리움 균주와 한국생명공학연구원 생물자원센터로부터 분양받은 표준균주를 가지고 분류 및 동정에 ERIC-PCR과 TAP-PCR을 이용한 RAPD-fingerprinting을 수행하였다. 그 결과 비피도박테리움 균주에 대한 속과 종단위의 분류가 가능하였으며, 실험에 사용된 모든 비피도박테리움 균주는 RAPD-fingerprinting 분석을 통해 유전적 다양성을 확인하였다. 또한 ERIC2와 TAP1 프라이머를 이용한 실험에서는 Bifidobacterium adolescenits 특이 유전자 단편을 확인하였으며 이는 B. adolescenits 균주의 동정에 유용할 것으로 사료된다.

Differentiation of Salmonella typhimurium from Gram-negative Intestinal Microbes by Randomly Amplified Polymorphic DNA (RAPD) Fingerprinting

  • Jin, Un-Ho;Chung, Tae-Wook;Kim, June-Ki;Nam, Kyung-Soo;Ha, Sang-Do;Kim, Cheorl-Ho
    • Journal of Microbiology
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    • v.38 no.1
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    • pp.8-10
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    • 2000
  • In order to rapidly identify and differentiate Salmonella typhimurium from the intestinal gram-negative bacteria, randomly amplified polymorphic DNA (RAPD) fingerprinting of Salmonella typhimurium was carried out using random primers designated OPA-13 (5'-CAGCACCCAC-3'), OPB-10 (5'-CGTCTGGGAC-3'), OPB-18 (5'-CCACAGCAGT-3'), and OPJ-10 (5'-AAGCCCGAGG-3'), and its patterns compared with 6 representive intestinal, gram-negative bacterial strains, Vibrio parahaemolyticus, V. vulnificus, Enterobacter cloacae, Escherichia coli O157:H7, Pseudomonas aeruginosa, and Proteus sp., which are often found in foods. S. typhimurium had unique and distinct fingerprinting patterns. RAPD fingerprinting is thus concluded to be a rapid and sensitive method for the identification of S. typhimurium compared to conventional culturing procedures or immunoassays.

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Audio Fingerprinting Using a Robust Hash Function Based on the MCLT Peak-Pair (MCLT 피크쌍 기반의 강인한 해시 함수를 이용한 오디오 핑거프린팅)

  • Lee, Jun-Yong;Kim, Hyoung-Gook
    • The Journal of the Acoustical Society of Korea
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    • v.34 no.2
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    • pp.157-162
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    • 2015
  • In this paper, we propose an audio fingerprinting using robust hash based on the MCLT (Modulated Complex Lapped Transform) peak-pair. In existing methods, the robust audio fingerprinting is not generated if various distortions occurred; time-scaling, pith-shifting and equalization. To solve this problem, we used the spectrum of the MCLT, an adaptive thresholding method for detection of prominent peaks and the novel hash function in the audio fingerprinting. Experimental results show that the proposed method is highly robust in various distorted environments and achieves better identification rates compared to other methods.

A Study on the Fingerprinting scheme without Trusted Third Party (신뢰기관 비참여의 핑커프린팅 기법에 관한 연구)

  • Yong, Seung-Lim
    • Journal of the Korea Society of Computer and Information
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    • v.14 no.7
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    • pp.81-88
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    • 2009
  • Fingerprinting scheme is a technique which supports the copyright protection to track redistributors of digital content using cryptographic techniques. These schemes enable the original merchant to identify the original buyer of the digital data by embedding fingerprints into digital contents. Asymmetric property of fingerprinting schemes is important to keep the buyer's privacy. In this paper, we propose a symmetric encryption based fingerprinting protocol without trusted third party. Our scheme enables the reduction of computational costs for the encryption using symmetric key encryption scheme. Since a trusted third party doesn't take part in making the fingerprint of each buyer, the protocol doesn't need to control the trusted third party and it is more secure against collusion attack.

Web Server Design For Web Page Fingerprinting (Web Page Fingerprinting을 위한 Web Server 구현)

  • Park, Su-Bin;Cho, Dong-Sub
    • Proceedings of the Korean Information Science Society Conference
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    • 2008.06b
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    • pp.375-379
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    • 2008
  • 디지털 핑거프린팅(Digital Fingerprinting) 기술은 구매자의 정보를 인지할 수 없는 방법으로 삽입하는 방법이다. 본 논문에서는 핑거프린팅 기법을 사용하여 웹 서버에 요청이 들어온 모든 웹 페이지에 핑거프린팅 기술의 조건을 충족시키는 방법으로, 정보를 삽입하여 보여 지는 웹 페이지의 변화 없이 사용자의 IP정보를 웹 페이지에 포함시켜 전송할 수 있는 알고리즘을 제시하고 웹페이지 핑거프린팅을 해주는 서버이용의 장점을 알아보도록 한다.

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A Robust Audio Fingerprinting Method Based on Segmentation Boundaries

  • Seo, Jin-Soo
    • The Journal of the Acoustical Society of Korea
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    • v.31 no.4
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    • pp.260-265
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    • 2012
  • A robust audio fingerprinting method is presented based on segmentation boundaries. In order to obtain robustness against linear speed changes, fingerprint extraction and matching are synchronized with the segmentation boundaries. Experimental results show that the proposed method is also robust against other common audio processing steps including low bit-rate compression, equalization, and time-scale modification.

Selective or Class-wide Mass Fingerprinting of Phosphatidylcholines and Cerebrosides from Lipid Mixtures by MALDI Mass Spectrometry

  • Lee, Gwangbin;Son, Jeongjin;Cha, Sangwon
    • Bulletin of the Korean Chemical Society
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    • v.34 no.7
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    • pp.2143-2147
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    • 2013
  • Matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a very effective method for lipid mass fingerprinting. However, MALDI MS suffered from spectral complexities, differential ionization efficiencies, and poor reproducibility when analyzing complex lipid mixtures without prior separation steps. Here, we aimed to find optimal MALDI sample preparation methods which enable selective or class-wide mass fingerprinting of two totally different lipid classes. In order to achieve this, various matrices with additives were tested against the mixture of phosphatidylcholine (PC) and cerebrosides (Cers) which are abundant in animal brain tissues and also of great interests in disease biology. Our results showed that, from complex lipid mixtures, 2,4,6-trihydroxyacetophenone (THAP) with $NaNO_3$ was a useful MALDI matrix for the class-wide fingerprinting of PC and Cers. In contrast, THAP efficiently generated PC-focused profiles and graphene oxide (GO) with $NaNO_3$ provided Cer-only profiles with reduced spectral complexity.