Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Genomic Fingerprinting Patterns of Bifidobacteria Isolated from Healthy Koreans Using ERIC-, TAP-, and BOX-PCR

건강한 한국인으로부터 분리된 비피도박테리아의 ERIC-, TAP-, BOX- 중합효소연쇄반응을 이용한 유전자 지문 분석

  • Published : 2008.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

유산균인 비피도박테리아는 사람과 동물에서 유익한 프로바이오틱 미생물로 알려져 있다. 본 연구에서는 이러한 비피도박테리아 균주의 분류를 위한 repetitive DNA element PCR fingerprinting (ERIC-또는 TAP-PCR)의 사용을 평가하였다. 사람분변으로부터 분리한 알려지지 않은 비피도박테리움 균주와 한국생명공학연구원 생물자원센터로부터 분양받은 표준균주를 가지고 분류 및 동정에 ERIC-PCR과 TAP-PCR을 이용한 RAPD-fingerprinting을 수행하였다. 그 결과 비피도박테리움 균주에 대한 속과 종단위의 분류가 가능하였으며, 실험에 사용된 모든 비피도박테리움 균주는 RAPD-fingerprinting 분석을 통해 유전적 다양성을 확인하였다. 또한 ERIC2와 TAP1 프라이머를 이용한 실험에서는 Bifidobacterium adolescenits 특이 유전자 단편을 확인하였으며 이는 B. adolescenits 균주의 동정에 유용할 것으로 사료된다.

Keywords

References

  1. Buchanon RE and Gibbons WE. The relationship between diet and rat fecal bacterial enzymes implicated in colon cancer, J Natl Cancer Inst 1976; 57: 371-375 https://doi.org/10.1093/jnci/57.2.371
  2. Choi SS, Kang BY, Chung MJ, Kim SD, Park SH, Kim JS, Kang CY and Ha NJ. Safety assessment of potential lactic acid bacteria Bifidobacterium longum SPM1205 isolated from healthy Koreans, J Microbiol 2005; 43: 493-498
  3. Collins MD, Rodrigues U, Ash C, Aguirre M, Farrow JAE, Martinezmurcia A, Phillips BA, Williams AM and Wallbanks S. Phylogenetic analysis of the genus Lactobacillus and related lactic acid bacteria as determined by reverse-transcriptase sequencing of 16S ribosomal-RNA, FEMS Microbiol Lett 1991; 77: 5-12 https://doi.org/10.1111/j.1574-6968.1991.tb04313.x
  4. Cusick SM and O'Sullivan DJ. Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria, Appl Environ Microbiol 2000; 66: 2227-2231 https://doi.org/10.1128/AEM.66.5.2227-2231.2000
  5. De Urraza PJ, Gomez-Zavaglia A, Lozano ME, Romanowski V and De Antoni GL. DNA fingerprinting of thermophilic lactic acid bacteria using repetitive sequencebased polymerase chain reaction, J Dairy Res 2000; 67:381-392 https://doi.org/10.1017/S002202990000426X
  6. Drake M, Spence CL and Swanson BG. Rapid detection and identification of Lactobacillus spp. in dairy products by using polymerase chain reaction, J Food Prot 1996; 59: 1031-1036 https://doi.org/10.4315/0362-028X-59.10.1031
  7. Englund S. IS900/ERIC-PCR as a tool to distinguish Mycobacterium avium subsp. paratuberculosis from closely related mycobacteria, Vet Microbiol 2003; 96: 277-287 https://doi.org/10.1016/j.vetmic.2003.08.004
  8. Fuller R. History and development of probiotics, In R. Fuller (ed.), Probiotics-The Scientific Basis, Champman and Hall, London 1992; pp. 1-18
  9. Gancheva A, Pot B, Vanhonacker K, Hoste B and Kersters K. A polyphasic approach towards the identification of strains belonging to Lactobacillus acidophilus and related species, Syst Appl Microbiol 1999; 22: 573-585 https://doi.org/10.1016/S0723-2020(99)80011-3
  10. Gevers D, Huys G and Swings J. Applicability of rep-PCR fingerprinting for identification of Lactobacillus species, FEMS Microbiol Lett 2001; 205: 31-36 https://doi.org/10.1111/j.1574-6968.2001.tb10921.x
  11. Gibson GR, Rastall RA and Fuller R. The health benefits of probiotics and prebiotics. In Fuller R and Perdigon G (Eds.), Gut Flora, Nutrition, Immunity and Health. Blackwell Publishing, Oxford 2003; pp. 52-70
  12. Gopal A, Shah NP and Roginski H. Bile tolerance, taurao-March 2008 Lee et al. : Genomic Fingerprinting of Bifidobacteria 7 chloate deconjugation and cholesterol removal by Lactobacillus acidophilus and Bifidobacterium, Milchwissenschaft 1996; 51: 619-623
  13. Homma N. Bifidobacteria as a resistance factor in human beings, Bifidobact Microfl 1998; 7: 35-43
  14. Kato I, Kobayashi S, Yokokura T and Mutai M. Antitumor activity of Lactobacillus casei in mice, Gann 1981; 72: 517-523
  15. Klein G., Park A, Bonaparte C and Reuter G. Taxonomy and physiology of probiotic lactic acid bacteria, Int J Food Microbiol 1998; 41: 105-125
  16. Kohwi T, Imai K, Tamura A and Hashimoto Y. Antitumor activity of Bifidobacterium infantis in mice, Gann 1978; 69: 613-618
  17. MacGowan AP, Odonaghue K, Nicholas S, McLauchlin J, Bennett PM and Reeves DS. Typing of Listeria spp. by random amplified polymorphic DNA (RAPD) analysis, J Clin Microbiol 1993; 38: 322-327
  18. asco L, Huys G, Gevers D, Verbrugghen L and Swings J. Identification of Bifidobacterium species using rep-PCR fingerprinting, Syst Appl Microbiol 2003; 26: 557-563 https://doi.org/10.1078/072320203770865864
  19. Mitsuoka T. Bifidobacteria and their role in human health, J Ind Microbiol 1990; 6: 263-268 https://doi.org/10.1007/BF01575871
  20. Modler HW. Bifidogenic factors-sources, metabolism and applications, Int Dairy J 1994; 4: 383-407 https://doi.org/10.1016/0958-6946(94)90055-8
  21. Modler HW, Mckeller RC and Yaguchi M. Bifidobacteria and bifidogenic factors. Can Inst Food Sci Technol J 1990; 23: 29-41 https://doi.org/10.1016/S0315-5463(90)70197-6
  22. National Committee for Clinical Laboratory Standards (NCCLS). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard-sixth edition. Approved document M7-A6. Wayne (PA) 2003
  23. Olive DM and Bean P. Principles and applications of methods for DNA based typing of microbial organisms, J Clin Microbiol 1999; 37: 1661-1669
  24. Park SY, Ji GE, Ko YT, Jung HK, Ustunol Z and Pestka JJ. Potentiation of hydrogen peroxide, nitric oxide and cytokine production in RAW 264.7 macrophage cells exposed to human and commercial isolates of Bifidobacterium, Int J Food Microbiol 1999; 46: 231-241 https://doi.org/10.1016/S0168-1605(98)00197-4
  25. Perdigon G, Macias ME, Alvarez S, Oliver G and Ruiz Holgado AA. Effect of perorally administered lactobacilli on macrophage activation in mice, Infect Immune 1986; 53: 404-410
  26. Pot B, Judwig W, Kersters K and Schleifer KH. Taxonomy of lactic acid bacteria. In Bacteriocins of Lactic Acid Bacteria: Microbiology, Genetics and Applications, L de Vuyst, and CJ Vandamme (Eds.), Blackie Academic and Professional, London 1994; pp. 13-90
  27. Pot B, Vandamme P and Kersters K. Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics. M. Goodfellow, and A.G. O'Donnell (Eds.), John Wiley and Sons Ltd., Chichester 1994; pp. 493-521
  28. Rafter JJ. The role of lactic acid bacteria in colon cancer prevention, Scand J Gastroenterol 1999; 30: 497-502 https://doi.org/10.3109/00365529509089779
  29. Rhee YK, Han MJ, Choi EC and Kim DH. Hypocholesterolemic activity of Bifidobacteria isolated from a healthy Korean, Arch Pharm Res 2002; 25: 681-684 https://doi.org/10.1007/BF02976944
  30. Salminen S, Deghton M and Gorbach S. Lactic acid bacteria in health and disease, In S. Salminen and A. von Wright (ed), Lactic Acid Bacteria, Marcel Dekker, Inc., NY 1974; pp. 199-226
  31. Sandery M, Coble J and Mckersie-Donnolley S. Random amplified polymorphic DNA(RAPD) profiling of Legionella pneumophila, Lett Appl Microbiol 1994; 19: 184-187 https://doi.org/10.1111/j.1472-765X.1994.tb00939.x
  32. Sekine K, Toida T, Saito M, Kuboyama M, Kawashima T and Hashimoto Y. A new morphologically characterized cell wall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice, Cancer Res 1985; 45: 1300-1307
  33. Sekine K, Watanabe-Sekine E, Toida T, Kasashima T, Kataoka T and Hashimoto Y. Adjuvant activity of the cell wall of Bifidobacterium infantis for in vivo immune responses in mice, Immunopharmacol Immunotoxicol 1994; 16: 589-609 https://doi.org/10.3109/08923979409019741
  34. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH and Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: Criteria for bacterial strain typing, J Clin Microbiol 1995; 33: 2233-2239
  35. Tynkkynen S, Satokari R, Saarela M, Mattila-Sandholm T and Saxelin M. Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electorphoresis in typing of Lactobacillu rhamnosus and L. casei strains, Appl Environ Microbiol 1999; 65: 3908-3914
  36. Vandamme P, Pot B, Gillis M, de Vos P, Kersters K and Swings J. Polyphasic taxonomy, a consensus approach to bacterial systematics, Microbiol Rev 1996; 60: 407-438
  37. Versalovic J, Schneider M, de Bruijn FJ and Lupski JR. Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction, Methods Mol Cell Biol 1994; 5: 25-40
  38. Welsh J and McClelland M. Fingerprinting genomes using PCR with arbitrary primers, Nucleic Acids Res 1990; 18: 7213-7218 https://doi.org/10.1093/nar/18.24.7213
  39. Yoon KY, Woodams EE and Hang YD. Probiotication of Tomato Juice by Lactic Acid Bacteria, J Microbiol 2004; 42: 315-318
  40. You HJ, Oh DK and Ji GE. Anticancerogenic effect of a novel chiroinositol containing polysaccharide from Bifidobacterium bifidum BGN4, FEMS Microbiology Letters 2004; 240: 131-136 https://doi.org/10.1016/j.femsle.2004.09.020