• 제목/요약/키워드: field-rearing

검색결과 103건 처리시간 0.024초

Molecular methods for diagnosis of microbial pathogens in muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae)

  • Gangavarapu Subrahmanyam;Kangayam M. Ponnuvel;Kallare P Arunkumar;Kamidi Rahul;S. Manthira Moorthy;Vankadara Sivaprasad
    • International Journal of Industrial Entomology and Biomaterials
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    • 제47권1호
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    • pp.1-11
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    • 2023
  • The Indian golden muga silkworm, Antheraea assamensis Helfer is an economically important wild silkworm endemic to Northeastern part of India. In recent years, climate change has posed a threat to muga silk production due to the requirement that larvae be reared outdoors. Since the muga silkworm larvae are exposed to the vagaries of nature, the changing climate has increased the incidence of microbial diseases in the rearing fields. Accurate diagnosis of the disease causing pathogens and its associated epidemiology are prerequisites to manage the diseases in the rearing field. Although conventional microbial culturing methods are widely used to identify pathogenic bacteria, they would not provide meaningful information on a wide variety of silkworm pathogens. The information on use of molecular diagnostic tools in detection of microbial pathogens of wild silk moths is very limited. A wide range of molecular and immunodiagnostic techniques including denaturing gradient gel electrophoresis (DGGE), random amplified polymorphism (RAPD), 16S rRNA/ITSA gene sequencing, multiplex polymerase chain reaction (M-PCR), fluorescence in situ hybridization (FISH), immunofluorescence, and repetitive-element PCR (Rep-PCR), have been used for detecting and characterizing the pathogens of insects with economic significance. Nevertheless, the application of these molecular tools for detecting and typing entomopathogens in surveillance studies of muga silkworm rearing is very limited. Here, we discuss the possible application of these molecular techniques, their advantages and major limitations. These methods show promise in better management of diseases in muga ecosystem.

Anti-Stress Effects of Ginsenoside Rg3-Standardized Ginseng Extract in Restraint Stressed Animals

  • Kim, Chung-Soo;Jo, Young-Jun;Park, Se-Ho;Kim, Hae-Jung;Han, Jin-Yi;Hong, Jin-Tae;Cheong, Jae-Hoon;Oh, Ki-Wan
    • Biomolecules & Therapeutics
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    • 제18권2호
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    • pp.219-225
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    • 2010
  • We tested whether ginsenosides $Rg_3$-standardized ginseng extract (RGE) has anti-stress effects in restraint-stressed animals. RGE increased time spent in the open arms and open arm entries in the elevated plus-maze test. In addition, RGE blocked the reduction of center zone distance and stereotypes behaviors in the open-field test. RGE also increased head dips in stressed mice, indicating anxiolytic-like effects. Stress decreased movement distance and duration, burrowing, and rearing frequency but increased face washing and grooming. RGE significantly reversed burrowing and rearing activity in stressed mice. In addition, we measured sleep architecture in restraint stressed rats using EEG recorder. Stress increased rapid eye movement (REM) sleep, but total sleep and non-rapid eye movement (NREM) sleep were not changed. RGE did not affect sleep architecture in stressed rats. These behavioral experiments suggest that RGE has anti-stress effects in restraint-stressed animal models.

시간일지법으로 분석한 도시와 농촌 가족의 토요휴업일 생활실태 (Comparison of Urban Families and Rural Families in terms of Life Styles on Saturday-with-no-school by Using Time Diary Method)

  • 오경숙;한유미
    • 한국생활과학회지
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    • 제18권1호
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    • pp.113-122
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    • 2009
  • This study aimed to examine the urban families and rural families in tenns of life styles on Saturday-with-no-school. The subjects were the 386 parents whose children were in the first or second grade in rural or urban area. The instruments of analysis were time diary method and parents' stress scale developed by Kim & Kang(l997). The main results were as follows: First, in comparison with urban children, rural children spent more time in viewing TV and playing computer but spend less time in sleeping. field trip and cleaning on Saturday-with-no-school. Second, the difference of child-rearing time between mothers and fathers was less marked in urban area than in rural area. Rural fathers spent more time in watching TV or playing computer, while rural mothers spent more time in feeding and bathing of their children, helping their children do homework, and total child-rearing. Third, unlike urban parents, rural parents ddid not have much parenting stress on Saturday-with-no-school. These results show that there is a need to focus on rural parents in the preparation for introduction of 5 days-school-week system.

새로 개발한 등족촉진제 ″부추긴″(BOO-CHOO-GHIN)의 효과에 관하여 (Study on the Effect of a New Mounting Promoter ″BOO-CHOO-GHIN″)

  • 이장낙;김정배;정태암;이상풍
    • 한국잠사곤충학회지
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    • 제17권2호
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    • pp.151-153
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    • 1975
  • 저자들이 상족노동력의 절감을 위해서 새로 개발한 등족촉진제 "부추긴"(Boo-Choo-Ghin)은, 1) 그 등족효과가 적어도 현재 일본에서 널리 시판되고 있는 등족촉진제 Repper의 경우 보다 뛰어난다. 2) 충질, 적질 및 체질에 대해서 아무런 나쁜 영향도 미치지 않는다. Boo-Choo-Ghin은 이상과 같은 뛰어난 등족족진효과를 가지는 이외에도 분말상이어서 다루기가 쉽다, 인차성이 없다. 인축에 대해서 무해하다, 값이 싸다, 등의 장점을 지니고 있어 등족촉진제로서의 실용적 가치가 매우 큰 것으로 믿어진다. 것으로 믿어진다.

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자녀를 둔 어머니의 출산과정 경험 (An Ethnographic Research Study on Childbearing Process of Mother with Children in Korea)

  • 김영희
    • 여성건강간호학회지
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    • 제7권3호
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    • pp.271-283
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    • 2001
  • The childbearing process is a sociocultural phenomenon of a woman who gives birth to a child as well as a biological phenomenon. The purpose of this ethnographic research study was to explore the experience of childbearing process of mothers with children from pregnancy to the 3 months postpartum in Korea and to understand deeply the perspectives of childbearing women reflected on Korean sociocultural values. A convenient sample of 10 childbearing women were observed from January to October 2000 through field work in Seoul, Korea. Data analysis was accomplished under ongoing process. The results of this study were as follows : The mothers with children experienced self-reflection, family relation, and physical adaptation during pregnancy. In self-reflection, all mothers experienced universality and diversity in their self-discovering process. The universal experiences were maturation, life with family and priority on maternal value between being a mother and a woman. The diverse experiences were taking a dual role of working mother, emotional drift of a resigned mother, and disheartened life of a mother who has two daughters. In family relation, the foundation of the new marital relationship were attained during childbearing process and sexual life were changed for the benefit of a healthy mother and a healthy baby. All mothers established friendly relations with their mothers, but established friendly or conflicting or constraining relations with their mother-in-laws due to husband based family culture. In physical adaptation, the informants endured well the physical discomfort and recognized general appearance change. Also maternal-fetal interaction occurred and mothers realistically felt motherhood and accepted themselves as mother-to-be. The mothers prepared for the best delivery, look for a safe childbirth center, newborn goods, endorsed family coping during hospitalization and responded labor pain to make it more endurable, less painful, fast passed owing to labor recognition of the natural process to be a mother. After childbirth, they felt emancipation, satisfaction, accomplishment, more easiness, actually feeling as mother-to-be, emptiness, and showed response to the sex of newborn. Their Sanhujori practice was different according to the Sanhujori environment including provider, place, time in postpartum and reflected on Sanhubyung. The mothers felt actually mother-to-be and happiness during lactation regardless of feeding pattern. These mothers had a different maternal image about rearing subjecthood through their child-rearing experience. But all mothers felt need for family support and social support. The universal rearing response were actual feeling of mother-to-be, a strenuous experience, a pride on child-rearing, confusion, reflecting marital relationship, and wondering rivalry among children. In conclusion, mother of all with children went through self-discovery, self-reflection and made connections with the family as a mother and as a woman simultaneously during the childbearing process. Therefore it is suggested when harmony and balance between a mother and a woman is accomplished, the woman will lead a healthy and high quality of life. Also, this study sought to confirm the sociocultural factors affecting the childbearing process from the perspectives of the women with children. Therefore health care providers must understand deeply the childbearing women with children based on this finding of and try a integrative approach with new ideology of maternity with biocultural perspectives in a clinical setting.

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미국선녀벌레 천적인 선녀벌레집게벌 대량사육 체계 (Mass rearing system for Neodryinus typhlocybae(Hymenoptera: Dryinidae) as a biological control agent of Metcalfa pruinosa)

  • 서미자;김정환;노현정;서보윤;조점래;박홍현
    • 환경생물
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    • 제40권4호
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    • pp.423-432
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    • 2022
  • 미국선녀벌레 생물적 방제를 위한 기생성 천적인 선녀벌레집게벌 대량사육 체계를 구축하였다. 선녀벌레집게벌 고치 생산은 기주인 미국선녀벌레 약충밀도와 기주식물의 재식밀도에 따른 차이를 보였지만, 뽕나무 기주식물 잎당 평균 5~8개, 주당 평균 70~150개가 생산되었다. 선녀벌레집게벌 암컷 평균 고치 길이는 6.01~6.46 mm, 수컷은 4.20~4.62 mm로 암수 간 통계적으로 유의미한 차이를 보였으며, 우화 전 고치상태에서의 암수 구분으로 선녀벌레집게벌을 효과적으로 방사할 수 있을 것으로 보인다. 반야외조건에서 선녀벌레집게벌 기생률은 평균 13~17%로, 방사 마리수에 따른 기생률 차이는 확인되지 않았으나 방사 마리수가 많을수록 미국선녀벌레 개체군밀도증가율이 낮아지는 경향을 보였다. 선녀벌레집게벌의 성비나 이화성은 기주의 영기에 따라 달라졌다. 기주의 영기가 낮을수록 이화성 비율이 증가했고, 4~5령 약충에 기생시 암컷의 비율이 높게 나타나 대량사육 시 개체조절이 가능할 것으로 판단된다.

한국의 하추잠작 안정을 위한 환경요인에 관한 연구 (Studies on the Environmental Factors Affecting the Cocoon Crops in Summer and Autumn in Korea)

  • 이상풍
    • 한국잠사곤충학회지
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    • 제16권2호
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    • pp.1-34
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    • 1974
  • 우리나라의 다회육 육잠체계를 확립하기 위해서 하추잠작을 안정시키기 위한 각종 요인 및 동요인이 실용형질에 미치는 경향을 구명코저 실시한 수개시험중 사육시기별 잠작에 관한 시험에서는 우선 우리나라에서 3회육 이상의 다회육을 할 경우 사육시기별 잠작의 정도를 구명코저 연간 11기를 4개장소, 3개년에 걸처 실시하였고, 결과에서 하추잠작이 불량했던 원인을 알고저 실시한 양잠농가의 추잠작 불안정 요인시험에서는 우리나라 전형적인 양잠농가로 추정되는 19호에 대한 주재 관찰조사 및 실험분석을 통한 실제의 불작요인을 조사하였다. 동조사에서 지적된 불작요인을 개선할 경우의 효과를 알고저 실시한 육잠기술 지도효과에서는 춘추 연40개 농가에게 불작요인을 개선 지도하였을 때 얻어지는 지도효과를 지도전년, 지도당년, 지도후년, 육잠기술 상, 하위 농가 및 춘추별로 인근 춘추 연758농가를 대조로 비교분석 하였다. 이상에서 간제되는 상엽질의 계절적 변화 추이를 알고저 시기별로 채취한 상엽분말을 동일시기 인공사료육을 통해서 양부의 판정을 시도하였고 이상에서 가장 큰 문제점으로 대두된 영양 및 온도조건이 하, 초추잠작에 있어서 누에 계량형질에 미치는 정도를 알고저 하잠시험에서는 치, 장잠별 엽질과 온도 등 4개요인을 하, 초추잠시기의 양부조건 2개수준(2$^4$), 초추잠시험에서는 하잠과 같은 4개요인에 변온 혹은 경엽구 1개수준을 추가한 3개수준(3$^4$)의 요인시험으로 실시했다. 이상에서 얻어진 시험들의 결과를 종합 요약하면 다음과 같다. 1. 사육시기별 잠작에 관한 시험 1)잠작의 일반적 경향은 춘잠은 소잠이 빠를수곡 양호하였으며 추잠은 관행소잠시기를 정점으로 하여 그 시기로부터 빠르거나 늦어질수록 점차로 불량하나, 빠를수록 급격하게 불량하다. 2) 우리나라의 잠기별 잠작은 춘(1~4기), 추(7~10기), 만추(11기), 하(5기) 초추(6기)잠 순으로 불량하였다. 3) 잠작과 영역접배를 고려한 다회 보통육은 1, 3, 8, 10기 등 4회육이 적합하고 조상 4회육은 10기 대신에 11기 대치로 무난하다. 2. 양잠농가의 추잠작 불안정 요인시험 1) 양잠농가의 수견량을 지배한 치명적인 요인은 잠실 잠구의 소독불충분에 의한 병원축적이며 2) 이차적 요인은 사육온습도 관리불량, 배게치기, 적숙엽 선정기술 불량 및 결상총과소 등이며 잠작과 경영구 요소는 관련이 없는것 같다. 3) 견층비율에는 뽕의 엽질이 가장 크게 영향되고, 다음으로 통원누적으로 인한 누에의 건강도에 영향한 것 같다. 3. 육잠기술 지도효과 1) 지도농가의 상자당 수견량 춘잠기 32.3kg, 추잠기 25.8kg으로서 대조 농가보다 각각 20%, 35%가 증수되어 연평균 29.0kg으로서 26%의 증수를 실증하였다. 2) 지도농가의 3등견 이상의 고치는 춘잠기 77.1%, 추잠기 83.7%로서 대조농가보다 각각 14.3%, 11.3%가 많았다. 3) 지도농가의 지도후년도의 상자당 수견중은 춘잠기 31.2kg, 추잠기 26.9kg, 연평균 28.9kg으로서 대조농가보다 각각 3%. 16%, 8%가 증수되였으므로 이는 기술지도의 잔류효과로 인정하였다. 4) 기술수준에 따른 잔류효과는 기술수준 상위농가는 0% 하위농가는 19%이었다. 4. 상엽질의 계절적 변화추이 1) 장잠기 상엽을 주재로 한 인공사료육의 결과는 춘> 만추≒하>추≒> 초추의 순으로 우수했다. 2) 치잠기 상엽을 주재로한 인공사료육의 결과는 춘, 만추, 추, 하, 초추 순위의 경향을 보였으나 유의차는 인정되지 않았다. 5. 하추잠작 흉작 요인시험 1) 초추잠 (1) 치잠기의 고온은 생존율과 수견량을 크게 감소케 한 요인이 었으며, (2) 장잠기의 온도는 고온순으로 생존율, 수견량 및 견질을 저해하였다. 따라서 장잠기의 적온유대가 양과 질의 양면에서 중요함이 인정되었다. (3) 치잠기 연엽과 경엽은 단견중을 다소 가볍게 하고 경과일수를 크게 연장케 하였으나 생존률, 수견량 및 견층중 등에는 영향을 미치지 않았다. (4) 장잠기의 경엽은 수량과 특히 견질을 크게 저해 하였으나 연엽, 경엽 모두 생존률에는 영향을 미치지 않았다. (5) 장잠기 온도를 변온으로 하였을 경우 치잠기 온도도 변온으로 한 것이 유리한 경향을 보였는바 평강한 문제는 금후 검토되어져야 할 것이다. 2)하 잠 (1) 치잠기의 고온은 수견량과 단견중을 크게 감소케하였으며 다음으로 생존율을 저해하였다. (2) 장잠기의 고온은 수견량, 생존율 및 견질을 크게 저해하였다.

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아버지의 부모효능감이 자녀의 학교생활적응에 미치는 영향: 가정에서 교육지원활동의 매개효과 (Effects of Father's Parental Efficacy on Children's School Life: Mediating Role of Father's Educational Support at Home)

  • 최혜영;이수현
    • Human Ecology Research
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    • 제55권1호
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    • pp.81-92
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    • 2017
  • Father participation in child rearing is gaining its importance in the field of child development. Previous literature consistently suggests the importance of parental efficacy and educational support on children's school life. However, studies which that focus exclusively on the role of the father's role are highly limited. Based on previous literature, we hypothesized that parental efficacy would directly and indirectly affect children's school life via parental educational support at home. We aimed to expand on the previous literature by exploring the relations within fathers. Participants were 662 fathers whose children attended elementary, middle, and high school. We measured parental efficacy, educational support at home, and their children's school life were measured. Descriptive statistics were first conducted to investigate the general features of participants as well as the correlations between study variables. The research model was analyzed using structural equation modeling in AMOS 20.0. Results proved the direct effect of father's parental efficacy on father's educational support at home as well as children's school life. Fathers' educational support at home mediated the effect of parental efficacy on children's school life. Our results suggests that fathers who perceive themselves as efficient parents provides a higher level of educational support at home for their children; consequently, children of those fathers in turn adjust better in school and achieve higher grades. Also, the father's parental efficacy also indirectly exerts positive effects on children's school life via heightened educational support at home. An importance of paternal participation and support in child rearing and education was highly suggested. Relevant policy issues regarding paternal educational support were discussed.

상족기 습도가 해서율, 견사장 및 생사량비율에 미치는 영향 (The Effect of Relative Humidity in the Mounting Time on the Reelability Ratio, Bave Length, and Percentage of Raw Silk)

  • 최진래;이광길
    • 한국잠사곤충학회지
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    • 제28권2호
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    • pp.74-78
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    • 1986
  • 누에 동육에 있어 여러 가지 환경요인 중 상족기 실외평균 습도가 견질에 미치는 영향을 조사하기 위하여 5년간 경남의 진양, 하동, 산청, 거창군과 경북의 선산, 상주, 영풍, 안동군에서 견검정 의뢰한 검정구 중 군, 연도, 춘추별, 각 50검정구씩 총 4,000건을 검사 항목별로 분석한 결과는 다음과 같다. 1. 상족기의 습도가 다습할수록 고치의 해상률이 낮았는데 특히 상대습도가 80% 이상에서 그 현상이 뚜렷하였다. 2. 고치의 해상률이 높을수록 견사장이 길고 생사량비율도 높았다. 3. 추잠기가 춘잠기에 비해 고치의 평균해상률은 높았으나 견사장은 짧고 생사량비율도 떨어지는 경향 이였다. 4. 평야지에서 생산된 고치가 산간지보다 견사장이 길고 생사량비율도 높았으나 해상률은 떨어지는 경향이었다.

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분심기음가미방(分心氣飮加味方)이 타면장애(唾眠障碍)와 정서성(情緖性)에 미치는 영향(影響) (The Effect of Punsimgieumgamibang on Sleep Disorder and Emotionality in Animals)

  • 허용석;김종우;황의완;김현택;박순권;김현주
    • 동의신경정신과학회지
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    • 제11권2호
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    • pp.43-52
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    • 2000
  • This sudy aimed to evaluate the effect of Punsimgieum-gamibang(分心氣飮加味方) on emotionality and sleep disorder. Punsimgieum(分心氣飮) has been used for insomnia, trembling, emotionality caused by strong mental stimulation and continuous stress in Oriental Medicine. And it was reported that Punsimgieum had anti-stress and anti-depression effect. Animals were devided into two groups : control group and Punsimgieum-gamibang group.Emotionality was tested in open-field with five indexes : walking, rearing, grooming, excretion and start latency. For the study of sleep disorder, after two groups had been given caffeine into abdomen, activity amount in animals was assessed for daytime and then the average percentage of sleep in two groups was calculated.The following results were observed.1. In peripheral and central partition, meditaion group walked more than control group. The difference between two groups was statistically significant.2. In rearing and grooming frequency, there was difference between two groups, but it was not statistically significant. 3. In grooming. excretion and start latency. there was no difference between two groups.4. There was no difference in the average percentage of sleep between two groups.

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