• Title/Summary/Keyword: field study

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Characterization of Sources of Resistance to Bacterial Spot in Capsicum Peppers (고추 세균성점무늬병 저항성 유전자원과 그 주요 특성)

  • Byeon, Si-Eun;Abebe, Alebel Mekuriaw;Jegal, Yoon-Hyuk;Wai, Khin Pa Pa;Siddique, Muhammad Irfan;Mo, Hwang-Sung;Yoo, Hee Ju;Jang, Kil-Su;Hwang, Ji-Eun;Jeon, Su-Gyeong;Lee, Su-Heon;Kim, Byung-Soo
    • Horticultural Science & Technology
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    • v.34 no.5
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    • pp.779-789
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    • 2016
  • A total of 33 accessions of pepper (Capsicum spp.), including previously reported and newly discovered sources of resistance to bacterial spot caused by Xanthomonas euvesicatoria, were evaluated for their resistance to bacterial spot. The selected accessions were then grown and their horticultural characteristics were recorded. In a test for hypersensitive resistance (HR) to four races (P1, P3, P7, P8) of the pathogen found in Korea, KC00939 and Chilbok No.2, which carry the Bs2 gene, exhibited a hypersensitive response to all four races, as expected. Chilbok No.3, which carries the Bs3 gene, showed a hypersensitive reaction to race 1 and 7, as expected. KC00939 exhibited a high ASTA color value and tolerance to multiple infections from a viral complex of Cucumber mosaic virus (CMV) and Broad bean wilt virus (BBWV). Thus, this accession represents a promising genetic resource for breeding cultivars with multiple disease resistance and strong red coloration. KC01327, KC01617, KC01015, KC01760, KC01779, KC01137, KC01328, KC01006, KC00127, KC01704, and KC00995 did not exhibit hypersensitivity but showed a high level of general resistance when evaluated by spray inoculation. KC01617, KC01760, KC01779, KC01137, KC01704, and KC01777 are newly identified sources of resistance to bacterial spot. The previously and newly identified sources of resistance to bacterial spot evaluated in this study, including information about their resistance to CMV and BBWV complex in the field, the contents of pungent and sweet taste components, and the color values of dry fruits, will be useful for breeding pepper cultivars with resistance to bacterial spot.

Ecological Health Assessments on Turbidwater in the Downstream After a Construction of Yongdam Dam (용담댐 건설후 하류부 하천 생태계의 탁수영향 평가)

  • Kim, Ja-Hyun;Seo, Jin-Won;Na, Young-Eun;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.40 no.1
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    • pp.130-142
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    • 2007
  • This study was to examine impacts of turbid water on fish community in the downstream of Yongdam Dam during the period from June to October 2006. For the research, we selected six sampling sites in the field: two sites were controls with no influences of turbid water from the dam and other remaining four sites were the stations for an assessment of potential turbid effects. We evaluated integrative health conditions throughout applications of various models such as necropsy-based fish health assessment model (FHA), Index of Biological Integrity (IBI) using fish assemblages, and Qualitative Habitat Evaluation Index (QHEI). Laboratory tests on fish exposure under 400 NTU were performed to find out impact of turbid water using scanning electron microscope (SEM). Results showed that fine solid particles were clogging in the gill in the treatments, while particles were not found in the control. This results indicate that when inorganic turbidity increases abruptedly, fish may have a mechanical abrasion or respiratory blocking. The stream health condition, based on the IBI values, ranged between 38 and 48 (average: 42), indicating a "excellent" or "good" condition after the criteria of US EPA (1993). In the mean time, physical habitat condition, based on the QHEI, ranged 97 to 187 (average 154), indicating a "suboptimal condition". These biological outcomes were compared with chemical dataset: IBI values were more correlated (r=0.526, p<0.05, n=18) with QHEI rather than chemical water quality, based on turbidity (r=0.260, p>0.05, n=18). Analysis of the FHA showed that the individual health indicated "excellent condition", while QHEI showed no habitat disturbances (especially bottom substrate and embeddeness), food-web, and spawning place. Consequently, we concluded that the ecological health in downstream of Yongdam Dam was not impacted by the turbid water.

Evaluation of Water Quality after Rehabilitation of Cheonggye Stream using AGP Test (조류성장잠재력 조사를 이용한 청계천 복원 이후 수질 평가)

  • Park, Myung-Hwan;Hwang, Soon-Jin;Suh, Mi-Yeon;Kim, Yong-Jae;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.40 no.2
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    • pp.234-243
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    • 2007
  • Algal growth potential (AGP) test was performed to evaluate the water quality and changes in phytoplankton communities before and after a heavy rain event at six sampling sites in Cheonggye Stream (St. 1 and 2), Jungnang Stream (St. 3 and 4), and Lower part of Han River System (St. 5 and 6) after rehabilitation of Cheonggye Stream, October 2005. To test AGP on each sampling site, cyanobacterium Microcystis aeruginosa was applied as a standard alga. Total nitrogen (TN) showed high values at Jungnang Stream, while St. 4 recorded highest values in this study. However, TN values of Cheonggye Stream and Lower Part of Han River showed similar levels. Total phosphate (TP) also showed high values at Jungnang Stream, while St. 4 recorded highest. However, TP in Cheonggye Stream were extremely low levels. Although chlorophyll-${\alpha}$ (chi-${\alpha}$) contents before the rain event were similar through the sampling sites, chl-${\alpha}$ after the rain increased dramatically at Jungnang Stream and Lower part of Ban River. In particular, after the rain, TP was the highest at St. 4, where Cyclotella sp. dominated the phytoplankton community. When compared with control, AGP values before the rain were comparatively low in all sites, while those after the rain highly increased with the dose-dependently of field water added, due perhaps to the increased nutrients by rainfall. Similar results were observed in Cheonggye Stream. Therefore, for the aesthetic fostering for the citizens, although Cheonggye Stream was Presently being sustained by treated water supply, they have a potential of outbreak of phytoplankton by the increased nutrients supply when a heavy rain comes.

Systems for Production of Calves from Hanwoo(Korean Cattle) IVM/IVF/IVC Blastocyst III. Vitrification and One-Step Dilution of Hanwoo Blastocyst (체외생산된 한우 배반포기배로부터 송아지 생스을 위한 체계 III. 한우 배반포기배의 초자화 동결과 1단계 융해)

  • Kim, E.Y.;Kim, D.I.;Park, N.H.;Weon, Y.S.;Nam, H.K.;Lee, K.S.;Park, S.Y.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
    • Korean Journal of Animal Reproduction
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    • v.23 no.4
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    • pp.293-301
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    • 1999
  • This study was to examine whether Hanwoo IVM/IVF/IVC blastocyst can be successfully survived in vitro/in vivo after vitrification and one-step dilution. For vitrification, blastocysts were serially exposed in glycerol (G) and ethylene glycol(EG) mixtures[10% (v Iv) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then they were loaded in the straw, placed in cold nitrogen vapor for 3 min. and plunged into L$N_2$(-196$^{\circ}C$). One-step dilution within the straw was done in $25^{\circ}C$ and 36$^{\circ}C$ water for about 5 min. and 3 min., respectively. Recovered embryos after one-step dilution were cultured in cumulus cell mono-layered drop for 48 h or were transferred into recipient cows. When the embryo survival in vitro was assessed to re-expanded and hatched rates at 24 hand 48 h after one-step dilution, the results of vitrified group (85.4, 43.8%) was high, although these results were significantly lower than normal development (100.0, 63.3%) of control group, respectively (P<0.001, P<0.05). When in vitro survival of vitrified groups according to developmental stage was compared, the results of fast developed embryos (expanded blastocyst and early hatching blastocyst stage) were significantly higher than those of delayed developed one (early blastocyst stage) after one-step dilution (early hatching: 88.0, 48.0%: expanded: 81.1, 45.3%; early: 66.7, 14.3%) (P<0.05). Also, in case of in vitro survival of vitrified groups according to embryo age (day 7, 8 and 9), when embryo age was younger, in vitro survival was significantly higher (day 7: 67.3, 34.5%; day 8: 76.9, 40.7%; day 9: 60.9, 23.9%)(P<0.05). Finally, when in vivo development potential of vitrified and one-step diluted Hanwoo blastocysts was examined, 4 of 8 recipient (50%) cows became confirmed pregnant. These results demonstrated that our vitrification and one-step dilution technique can be applied easily and effectively on field trial without the equipment and embryological skills required for conservative dilution and transfer.

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Effect of Electric Purse Conditions on the Fusion and Development Embryos Produced by Ear Cell Nuclear Transfer in Brindle Coated Hanwoo (Korean Cattle) (칡소의 귀세포를 이용한 핵이식에서 전기융합조건이 융합 및 배발달에 미치는 영향)

  • 최은주;이호준;민관식;김창근;정영채;윤종택
    • Korean Journal of Animal Reproduction
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    • v.27 no.1
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    • pp.87-93
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    • 2003
  • This study was conducted to investigate the effects of embryo development by fusion condition on the nuclear transfer with brindle coated cow's ear cells. Ear cells were transferred into an enucleated oocyte and fused with cytoplasm in the fusion condition with 1.9kv/cm, 2.0kv/cm, 2.1kv/cm each 10 and 20ug duration Nuclear transfer embryo were activeted with a combination of 5ug/ml and 1.9mM 6-DMAP (4min, 4h). Fusion rate was 51∼68% range among fusion condition (1.9, 2.0, 2.1kv/cm; 10, 20us). But, cytoplasm lysis rate was increased by higher electric condition (0∼51.8% range). Each parameter's cleavage and blastocyst formation rate were 1.9kv/cm for 10us (75.8 and 19.5%), 20us (69.8 and 48.6%), 2.0kv/cm for 10us (76.9 and 20.0%), 20 us (68.5 and 40.9%), 2.1kv/cm for 10us (70.5 and 44.2%), 20 us (68.5 and 27.0%). We compared the effectiveness of cloning for between brindle coated cow's ear cells and Hanwoo fetal fibroblast cells. There was no significant differences in the fusion rate and developmental rate to the blastocyst stage. After transfer of blastocysts derived from nuclear transfer embryos, pregnancy rates of the Hanwoo fetal fibroblast cells and brindle coated cow's ear cells were checked pregnant on day 60 as assessed by ultrasonography, 40% (2/5) and 15.8% (3/19), respectively. This studies conclude that brindle coated cow's ear cells have the developmental potentiality to term by nuclear transfer. These results demonstrate that the increased the field strength was to be profitable for development of blastocyst or reduce of cytoplasm's damage than increasing the pulse duration.

Fifty Years of the Korean Journal of Clinical Laboratory Science: About Name and KCI Registration (대한임상검사과학회지 50년사: 명칭과 KCI 등재에 대하여)

  • Koo, Bon-Kyeong;Sung, Ho Joong;Rhee, Ki-Jong;Yang, Byoung-Seon;Joo, Sei Ick;Choi, Seung-Gu;Jang, In-Ho;Yang, Man-Gil
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.3
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    • pp.187-202
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    • 2017
  • The Korean Society for Clinical Laboratory Science publishes the Korean Journal of Clinical Laboratory Science (KJCLS) as the official journal for the Korean Association of Medical Technologists. This year, 2017, marks the 50th anniversary of KJCLS. The original name was "the Korean Journal of Medical Technologists" (Volume 1, 1967~Volume 26, 1994), which was replaced with the "Korean Journal of the Clinical Laboratory Science" (Volume 27, 1995~Volume 35, 2003) and "the Korean Journal of Clinical Laboratory Science" (Volume 26, 2004~Present). The purpose of this study was to provide a framework for further development of KJCLS. In 1998, the National Research Foundation of Korea began the journal assessment service. The major domestic databases are KCI, KOFST, and KAMJE; the major international databases are SCI Index; SCI Core, SCI-Extended), SCOPUS, MEDLINE, and PUBMED. KJCLS has been registered with KCI of the National Research Foundation of Korea since 2014. More than 1,300 articles have been published, which all have made tremendous contributions to the field. To index KJCLS in the journal databases, we measured the value to understand the vocational level and social position. KJCLS is faced with a great challenge; immense effort will be needed to accomplish the original goal of KJCLS to become an internationally recognized journal. Ultimately, the goal of the Korean Society for Clinical Laboratory Science and the Korean Association of Medical Technologists should be to register KJCLS in databases such as KCI and SCOPUS.

Suggestions for Invention Gifted Education Based on the Awareness of Teachers and Professionals Related to the Invention Gifted Education (발명영재 교원 및 전문직 인식에 기반한 발명영재교육의 방향 탐색)

  • Park, Ki-Moon;Lee, Kyu-Nyo;Lee, Byung-Wook;Na, Young-Min;Lee, Kyung-Pyo;Son, Da-Mi;Lee, Sang-Hyun
    • Journal of Gifted/Talented Education
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    • v.22 no.1
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    • pp.1-21
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    • 2012
  • The objective of this study is to suggest improvement plan for invention gifted education based on the awareness of teachers and professionals who related to the invention gifted education for the expansion and development of invention gifted education through improvement of relevant problems. To this end, invention gifted education system and its operational status were analyzed, and questionnaire survey on the awareness of the development plan and satisfaction level was conducted, targeting professionals related to the invention gifted education and teachers in charge of invention gifted education classes or gifted education center. The research results are as follows. First, the level of satisfaction on the invention gifted education was greater than normal (M=3.0) in general, but in the field of 'educational materials', 'teacher training programs' and 'human and material support system of support agencies', the level of satisfaction was relatively low, which requires expansion of the support. Second, it is necessary for Korean Intellectual Property Office and Korea Invention Promotion Association to designate and establish specialized research institution to play a key role in enhancing development and efficiency of invention gifted education. As a result of the questionnaire survey, it turned out that expectation and necessity of the specialized research agency was highly recognized. In particular, demand for 'research and development of gifted education method and materials' and 'research and development of teacher training materials and implementation of teacher training' was high among the key areas of the specialized research institution. Third, teachers and professionals related to the invention gifted education responded that 'invention knowledge' in the areas of invention knowledge and thinking and 'entrepreneurship' in the area of invention attitude was somewhat low toward the question on the level of the 9 characteristics of gifted students with invention talents which current beneficiaries of invention gifted education have, which leads to conclusion that review on the model for the selection of gifted children with invention talents as well as research and development of invention gifted education program to enhance characteristics with low levels is required. If long-term development plans and initiatives are deduced based on this, an effective framework for the invention gifted education will be established in the near future. In addition, it is expected that the differentiated political visions and goals will be established in connection with master plan for the promotion of gifted education.

A Comparative Analysis between Photogrammetric and Auto Tracking Total Station Techniques for Determining UAV Positions (무인항공기의 위치 결정을 위한 사진 측량 기법과 오토 트래킹 토탈스테이션 기법의 비교 분석)

  • Kim, Won Jin;Kim, Chang Jae;Cho, Yeon Ju;Kim, Ji Sun;Kim, Hee Jeong;Lee, Dong Hoon;Lee, On Yu;Meng, Ju Pil
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.35 no.6
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    • pp.553-562
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    • 2017
  • GPS (Global Positioning System) receiver among various sensors mounted on UAV (Unmanned Aerial Vehicle) helps to perform various functions such as hovering flight and waypoint flight based on GPS signals. GPS receiver can be used in an environment where GPS signals are smoothly received. However, recently, the use of UAV has been diversifying into various fields such as facility monitoring, delivery service and leisure as UAV's application field has been expended. For this reason, GPS signals may be interrupted by UAV's flight in a shadow area where the GPS signal is limited. Multipath can also include various noises in the signal, while flying in dense areas such as high-rise buildings. In this study, we used analytical photogrammetry and auto tracking total station technique for 3D positioning of UAV. The analytical photogrammetry is based on the bundle adjustment using the collinearity equations, which is the geometric principle of the center projection. The auto tracking total station technique is based on the principle of tracking the 360 degree prism target in units of seconds or less. In both techniques, the target used for positioning the UAV is mounted on top of the UAV and there is a geometric separation in the x, y and z directions between the targets. Data were acquired at different speeds of 0.86m/s, 1.5m/s and 2.4m/s to verify the flight speed of the UAV. Accuracy was evaluated by geometric separation of the target. As a result, there was an error from 1mm to 12.9cm in the x and y directions of the UAV flight. In the z direction with relatively small movement, approximately 7cm error occurred regardless of the flight speed.

Study on Adaptation, Dry Matter Yield and Nutrient Value of Kenaf(Hibiscus cannabinus L.) on Jeju Province (양마의 제주지역 지역 적응성, 생산성 및 사료가치에 관한 연구)

  • Hwang, Kyung-Jun;Kim, Moon-Cjul;Kang, Si-Young;Yu, Jang-Geal;Song, Sang-Teak;Park, Nam-Geon;Kim, Jong-Ha
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.4
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    • pp.287-296
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    • 2002
  • A field trial was conducted from May to November 2000 at four regions of Jeju province(Jeju, Seogwipo, Susan Seongsan and Keumak Hallim) to estimate adaptation, dry matter yield and nutrient value of kenafs(Everglades 41 and China Choung-pi 3) and a sorghum-sudangrass hybrid(Pioneer 947). Total dry matter(DM) yields of sorghum-sudangrass hybrid Pioneer 947, kenaf Everglades 41 and kenaf China Chong-pi 3 were 7,313, 4,653 and 5,238 kg/ha(P<0.05), respectively, kenaf China Chong-pi 3 being the highest. The region specific dry matter(DM) yields of Jeju, Seogwipo, Seongsan and Hallim area were 5,040, 5,663, 7,283 and 4,950kg/ha, respectively, showing that Seogwipo was the highest. Average DM yields of two kenaf varieties were 4,946, and 3,246 kg/ha for 1st time-cutting and 2nd time-cutting, respectively. The average dry matter(DM) yield of kenaf was significantly higher in the 1st time-cutting than the 2nd time. No significant difference in neutral detergent fiber(NDF) and acid detergent fiber(ADF) contents was found among three varieties. The average crude protein(CP) content of kenaf was higher than that of sorghum-sudangrass hybrid(P<0.05) and was higher in 2nd time-cutting than in 1st time-cutting(P<0.05). There were no significant difference in the heavy metal content of soil either between the sorghum hybrid and kenafs or between two kenaf varieties. In conclusion, the average dry matter(DM) yield of kenaf was lower than that of sorghum hybrid, Contrarily the crude protein(CP) content of kenafs was higher than that of the sorghum hybrid. The average dry matter(DM) yield of kenaf showed higher in 1st time-cutting than 2nd time-cutting, while the crude protein(CP) content of kenafs decreased with time.

Studies on the Seed Production of Festuca arundinacea Schreb I. Effect of nitrogen Fertilization level and method of its application on the seed production of level and method of its application on the seed production of Festuca arundinacea (톨 페스큐의 종자생산에 관한 연구. I. 질소비료의 시비수준 및 분시방법이 톨 페스큐 ( Festuca arundinacea S. ) 의 종자생산에 미치는 영향)

  • 박근제;권두종;이종열;양종성
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.2
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    • pp.100-105
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    • 1985
  • To find out the optimum level of nitrogen fertilization and better distribution of application time for seed production of Festuca arundianacea S. (var. Alta), this filed experiment was performed at Livestock Experiment Station in Suweon, during 1979 to 1981. The treatments used in this study were three nitrogen fertilizing levels (120, 180 and 240kg/ha) and four different methods of nitrogen distribution (i. single application of whole amount in early Spring, ii. 50 percent each in Autumn and early Spring, iii. 50 percent each in early Spring and at begin of heading stage and iv. 50 percent in Autumn and 25 percent in early Spring and at begin of heading stage respectively). The experiment was arranged as a split-plot design with 4 replications and performed at the experimental field of the Livestock Experiment Station in Suweon, during 1979 to 1981. The results obtained are summarized as follows: 1. Date of heading stage of Festuca arundinacea was about May 21 and that of flowering stage was June 1 to 3. The optimum stage for the seed harvesting of Festuca arundinacea (var. Alta) was June 25 about 22 to 23 days after full flowering stage. Average plant height was about 127cm and the panicle length, 24cm. 2. 1000 grain weight was 2.72g and the number of panicles were 85 to 107 per square meter. 3. The mean seed wield for two years was 678.8kg/ha with the average of whole treatments and 781.9kg/ha with the best treatment (50 percent in Autumn and 25 percent in early Spring and at begin of heading stage with 240kg/ha respectively). 4. The average germination rate of harvesting seeds was 87.0 percent and it was increasing trend according to frequent application of fertilizer. 5. The average DM yield of aftermath seed harvesting was 6155kg/ha with two cut, and it was the largest DM yield from the higher nitrogen level and also from the single application in early Spring.

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