Korean Journal of Animal Reproduction (한국가축번식학회지)
- Volume 23 Issue 4
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- Pages.293-301
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- 1999
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- 1226-5284(pISSN)
Systems for Production of Calves from Hanwoo(Korean Cattle) IVM/IVF/IVC Blastocyst III. Vitrification and One-Step Dilution of Hanwoo Blastocyst
체외생산된 한우 배반포기배로부터 송아지 생스을 위한 체계 III. 한우 배반포기배의 초자화 동결과 1단계 융해
- Kim, E.Y. (Maria Infertility Medical Institute) ;
- Kim, D.I. (Hanwoo Improvement Center NLCF) ;
- Park, N.H. (Hanwoo Improvement Center NLCF) ;
- Weon, Y.S. (Hanwoo Improvement Center NLCF) ;
- Nam, H.K. (Maria Infertility Medical Institute) ;
- Lee, K.S. (Maria Infertility Medical Institute) ;
- Park, S.Y. (Maria Infertility Medical Institute) ;
- Yoon, S.H. (Maria Infertility Clinic) ;
- Park, S.P. (Maria Infertility Medical Institute) ;
- Chung, K.S. (College of Animal Husbandry, Konkuk University) ;
- Lim, J.H. (Maria Infertility Medical Institute)
- Published : 1999.12.01
Abstract
This study was to examine whether Hanwoo IVM/IVF/IVC blastocyst can be successfully survived in vitro/in vivo after vitrification and one-step dilution. For vitrification, blastocysts were serially exposed in glycerol (G) and ethylene glycol(EG) mixtures[10% (v Iv) G for 5 min., 10% G plus 20% EG (v/v) for 5 min., and 25% G plus 25% EG (v/v) for 30 sec.] which is diluted in 10% FBS added D-PBS. And then they were loaded in the straw, placed in cold nitrogen vapor for 3 min. and plunged into L
본 실험은 체외 생산된 한우 배반포기배가 초자화 동결과 1- 단계 융해 후 체외 / 체내에서 성공적으로 생존할 수 있는지의 여부를 확인하고자 실시하였다. 초자화 동결은 glycerol (G) 또는 / 과 ethylene glycol(EG), 그리고 10% FBS가 들어있는 D- PBS 동결액을 이용하여 배반포기배를 10% G 에서 5분, l0%G와 20% EG 혼합액에서 5분 그리고 2S%G와 2S% EG 혼합액에서 30 초간 노출시켰으며, straw에 넣은 후 질소 증기에 3분간 쐬고 액화질소에 침지함으로써 완성하였다. 1-단계 융해는 straw 자체를