• Research in Plant Disease
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• v.16 no.2
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• pp.121-124
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• 2010

Immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) was applied to the detection of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus (KGMMV), and Zucchini green mottle mosaic virus (ZGMMV) on Cucurbitaceae crops. These seed-borne tobamoviruses were accurately detected from the infected leaves and seeds by IC-RT-PCR. This method was estimated to be about 100 times more sensitive than ELISA, and also it allowed the direct confirmation of ELISA results by using the captured antigens from a completed ELISA microwell. This convenient and reliable method could be used routinely for large-scale field surveys or seed tests of Cucurbitaceae crops.

• Korean Journal of Soil Science and Fertilizer
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• v.48 no.5
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• pp.528-534
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• 2015

Green manure crops have been well recognized as the alternative for chemical fertilizer, especially N fertilizer, because of its positive effect on soil and the environment. Hairy vetch and green barley are the most popular crops for cultivation of rice in paddy field. This study was conducted to evaluate effects of hairy vetch and green barley on soil microbial community and chemical properties during short-term application (three years). For this study, treatments were composed of hairy vetch (Hv), green barley (Gb), hairy vetch + green barley (Hv+Gb), and chemical fertilizer without green manure crops (Con.). Hv+Gb treatment showed the highest microbial biomass among treatments. Principal component analysis (PCA) showed that PC1 (73.0 %) was affected by microbial biomass and PC2 (21.5 %) was affected by fungi, cy19:0/18:$1{\omega}7c$ (stress indicator). Combined treatment with hairy vetch and green barley could be more efficient than green manure crop treatment as well as chemical fertilizer treatment for improvement of soil microorganisms.

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.143-149
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• 2003

This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacerium-mediated transformation. Twenty-five transgenic plants T$_{0}$ plants, back cross (BC$_1$) populations to wild type and F$_1$plants between different T$_{0}$ plants were prepared, and polymerase chain reaction(PCR), PCR-Southern blot analysis, and field test with 0.1% Basta treatment were done. The results of PCR, PCR-Southern blot hybridization, and field test indicated that NPTII and bar gene introduced into the genome of petuina plants were stably transmitted to their progenies, and conferred the plants resistance to herbicide, Basta.sta.

• Proceedings of the KSRS Conference
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• 2008.10a
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• pp.151-154
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• 2008

Information on the area and spatial distribution of crop fields is needed for biomass production, arrangement of water resources, trace gas emission estimates, and food security. The present study aims to monitor crops status during the growing season by estimating its aboveground biomass and leaf area index (LAI) from field reflectance taken with a hand-held radiometer. Field reflectance values were collected over specific spectral bandwidths using a handheld radiometer(LI-1800). A methodology is described to use spectral reflectance as indicators of the vegetative status in crop cultures. Two vegetation indices were derived from these spectral measurements. In this paper, first we analyze each spectral reflectance characteristics of vegetation in the order of growth stage. Vegetation indices (NDVI, GNDVI) were calculated from crop reflectance. And assess the nature of relationships between LAI and VI, as measured by the in situ NDVI and GNDVI. Among the two VI, NDVI showed predictive ability across a wider range of LAI than did GNDVI. Specific objectives were to determine the relative accuracy of these two vegetation indices for predicting LAI. The results of this study indicated that the NDVI and GNDVI could potentially be applied to monitor crop agriculture on a timely and frequent basis.

• Journal of Korean Society of Rural Planning
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• v.2 no.2
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• pp.81-90
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• 1996

In this paper, the basic planning for the creation of consolidated agricultural institutions of Choongcheongnam -Do and its management schemes were established. These institutions, including rural development office, farmers'training institution, original seeding field of farm crops, agriculturial information center, packing design center, agricultural machinery repair center, agricultural scientific conference hall, agricultural museum, agricultural park, farmers'hall and event plaza, are planned to be created. The required area for the site is 1,246,781 m2. 281-22 Jongkyung-Ri, Sinam-Myun, Yesan-Gun, Choongcheongnam-Do and its surroundings is a proper site for the creation of'consolidated agricultural institution because the actual conditions of the soil, traffic network, irrigation and drainage, tourist routes and human resources are very good. The layout of the facilities that would comprise an agricultural institution was organized in consideration of the functions and roles of each facility and the relationships among facilities in this consolidated agricultural institution. It is desirable that the headquaters of consolidated agricultural institution oversees the management department, the planning $.$ administration $.$ information department, the rural development office, the farmers'training institution, the original seeding field of farm crops and the agricultural park.

• The Plant Pathology Journal
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• v.37 no.6
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• pp.512-520
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• 2021

In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.296-303
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• 2022

The cucurbit powdery mildew (CPM) caused by different fungal species is a major concern for cucurbit crops around the world. In Argentina CPM constitutes the most common and damaging disease for cucurbits, especially for squash crops (Cucurbita moschata). The present study displays initial insights into the knowledge of the disease in western Argentina, including the determination of the prevalent species causing CPM, as well as the evaluation of the resistance of squash cultivars and breeding lines. Fungal colonies were isolated from samples collected in Mendoza province, Argentina. A field trial was also performed to assess the resistance of five squash accessions, including commercial cultivars and breeding lines. The severity of CPM was analyzed and epidemiological models were built based on empirical data. The morphological determinations and analysis with specific molecular markers confirmed Podosphaera xanthi as the prevalent causal agent of CPM in Mendoza. The results od the field trial showed differences in the resistance trait among the squash accessions. The advanced breeding line BL717/1 showed promising results as source of CPM resistance for the future development of open pollinated resistant cultivars, a crucial tool for an integrative control of the disease.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.3
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• pp.225-233
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• 1991

This study was conducted to evaluate the potential of indigenous vesicular-arbuscular mycorrhizal fungi(VAMF) in the rhizosphere soil of horticultural crops grown under greenhouse and open-field condition, in the southern area of Kores. Soil samples collected from the rhizosphere of some sellected horticultural crops, such as cucumber, hot pepper, lettuca, tomato and eggplant grown under greenhouse or open-field condition. All tested crops are considered as mycorrhizal plants. The infection rate of horticultural crops investigated ranged from 38% to 70%, hot pepper and eggplant grown under greenhouse condition showed the highest infection being 66.0% and 70.0%, respectively. Spore densities were from 4.8 to 20.0g-1 on dried soil basis. Spore densities of VAMF in the rhizosphere soils under greenhouse condition were higher than that of open-field conditions. The highest distribution of spores in diameter ranged from $75{\mu}m$ to $106{\mu}m$ in the rhizosphere soil of lettuce, cucumber and tomato while those in hot pepper and eggplant ranged from $75{\mu}m$ to $250{\mu}m$. Glomus sp.-type spores predominated in the slightly acid soil(pH 6.3), while Acaulospora sp.-type spores greatly predominated in the very strongly acid field(pH 4.9).

• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.467-472
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• 2009

This study was conducted to break down the self-incompatibility of Astragalus membranaceus Bunge by old-flower pollination, NaCl and $CO_2$ treatment. The old-flower pollination in green house produced fertilization during the 1st and 2nd day after flowering from early September to early October, but almost no fertilization in late August. The most successful pollination occurred in late September at the 1st day after flowering when pod setting was 33.3% and that of seed set was about 86.2%. The old-flower pollination in field showed pod setting for the control group from late August to early October, but no seed set except the days of mid-September and late September. The most successful pollination occurred in late September during the 2nd day of flowering when the percentage of pod setting was 39% and that of seed set was 94.9%. The wrapping in field set pods from late August to early October, but did not set seeds except in mid and end of September. The percentage of pods was 39% and percentage of seeds was 94.9% about flowering after first day in end of September. by field culture. The best result from NaCl treatment was achieved when 1% NaCl treatment in green house produced 21.3% of pod setting in early October and 66.7% of seed set in late September. In field, NaCl 5% treatment produced best result with 7.3% of pod setting and 90.9% of seed set in mid-September. No differences were observed between the $CO_2$ 700ppm treatment and the control group.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.18-28
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• 2021

The growth period and productivity of cropping system of winter wheat-rice, winter wheat-bean and winter wheat-grain corn for 4 years from 2015 to 2018 were compared at the experimental field of National Institute of Crop Science in Miryang city. The harvest period of winter wheat was in mid-June, and summer crops were sown (transplanted) in late June. In transplanting of rice in late June, there was no difficulty in securing the heading of panicle and the yield of rice, but there was a lot of trouble in sowing wheat in proper time because the harvest time of rice was delayed to early November due to late maturity of rice, particularly in the mid-late maturing cultivar. There was no problem in soybean planting after winter wheat because the proper period of soybean planting is late-June. In addition, there was no problem in winter wheat sowng after soybean because the maturity period of soybean was mid-October. Selection of grain maize in double cropping with winter wheat in terms of growing periods, was desirable because grain maize had the fastest maturity among summer crops. In double cropping of winter wheat-summer crops, wheats combined with soybean and grain maize showed stable yields during three years, but there was a risk of yield declines in the wheat combined with rice in heavy rainfall year. It was possible to secure high yields in three summer crops as yields of rice, soybean, and corn were 600, 350, and 800 kg/10a, respectively. Summer crops with medium maturity was recommended because of no significant difference in yield between medium maturity and medium-late maturity cultivar. Soil physical properties were improved in soils cultivated with soybean and grain maize. Therefore, It was thought that double cropping systems of winter wheat with soybean and grain maize were superior to that of winter wheat with rice in terms of connecting period between winter wheat - summer crops and improvement of soil physical properties, and total income, particularly in soybean.