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http://dx.doi.org/10.5010/JPB.2003.30.2.143

Expression and Inheritance of bar Gene in Petunia hybrida Transformed with Agrobacterium  

Ha, Young-Min (Department of Crops Biotechnology, Jinju National University)
Kim, Jong-Chul (Division of Applied Life Science, Gyeongsang National University)
Lee, Sang-Woo (Department of Horticulture, Gyeongsang National University)
Lee, Shin-Woo (Department of Crops Biotechnology, Jinju National University)
Kim, Zhoo-Hyeon (Department of Horticulture, Gyeongsang National University)
Publication Information
Journal of Plant Biotechnology / v.30, no.2, 2003 , pp. 143-149 More about this Journal
Abstract
This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacerium-mediated transformation. Twenty-five transgenic plants T$_{0}$ plants, back cross (BC$_1$) populations to wild type and F$_1$plants between different T$_{0}$ plants were prepared, and polymerase chain reaction(PCR), PCR-Southern blot analysis, and field test with 0.1% Basta treatment were done. The results of PCR, PCR-Southern blot hybridization, and field test indicated that NPTII and bar gene introduced into the genome of petuina plants were stably transmitted to their progenies, and conferred the plants resistance to herbicide, Basta.sta.
Keywords
Back cross; Basta circledR herbicide; PCR-Southern blot;
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