The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Genetic Diversity of Orobanche cumana Populations in Serbia

  • Received : 2021.04.29
  • Accepted : 2021.08.12
  • Published : 2021.12.01
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Abstract

In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

Keywords

Acknowledgement

The authors declare that they have no conflict of interests. This work was supported by the Ministry of Education and Science, and Technological development of Republic of Serbia (Contract No. 451-03-9/2021-14/200010).

References

  1. Acimovic, M. 1977. Distribution of important sunflower diseases in Yougoslavia. Institute of Field and Vegetable Crops, Novi Sad, Yougoslavia. 17 pp.
  2. Atanasova, R., Batchvarova, R., Todorovska, E. and Atanassov, A. 2005. Molecular study of broomrape (Orobanche spp.) by RAPD analyses. Biotechnol. Biotechnol. Equip. 19:51-60. https://doi.org/10.1080/13102818.2005.10817227
  3. Benharrat, H., Delavault, P., Theodet, C., Figureau, C. and Thalouarn, P. 2000. rbcL plastid pseudogene as a tool for Orobanche (subsection Minores) identification. Plant Biol. 2:34-39. https://doi.org/10.1055/s-2000-9457
  4. Bilgen, B. B., Barut, A. K. and Demirbas, S. 2019. Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers. Turk. J. Bot. 43:38-47. https://doi.org/10.3906/bot-1807-71
  5. Calderon-Gonzalez, A., Pouilly, N., Munos, S., Grand, X., Coque, M., Velasco, L. and Perez-Vich, B. 2019. An SSRSNP linkage map of the parasitic weed Orobanche cumana Wallr. including a gene for plant pigmentation. Front. Plant Sci. 10:797. https://doi.org/10.3389/fpls.2019.00797
  6. Castejon-Munoz, M., Suso, M. J., Romero-Munoz, F. and Garcia-Torres, L. 1991. Isoenzymatic study of broomrape (Orobanche cernua) populations infesting sunflower (Helianthus annuus). In: Proceedings of the 5th International Symposium of Parasitic Weeds, eds. by J. K. Ransom, L. J. Musselman, A. D. Worsham and C. Parker, pp. 313-319. International Maize and Wheat Improvement Center, Nairobi, Kenya.
  7. Delavaault, P. and Thalouarn, P. 2002. The obligate root parasite Orobanche cumana exhibits several rbcL sequences. Gene 297:85-92. https://doi.org/10.1016/S0378-1119(02)00868-5
  8. Dor, E., Plakhine, D., Joel, D. M., Larose, H, Westwood, J. H., Smirnov, E., Ziadna, H. and Hershenhorn, J. 2020. A new race of sunflower broomrape (Orobanche cumana) with a wider host range due to changes in seed response to strigolactones. Weed Sci. 68:134-142. https://doi.org/10.1017/wsc.2019.73
  9. Duca, M., Joita-Pacureanu, M., Port, A., Martea, R., Boicu, A., Risnoveanu, L. and Clapco, S. 2019. Genetic diversity analysis of sunflower broomrape populations from Republic of Moldova using ISSR markers. Rom. Agric. Res. 37:89-97.
  10. Duca, M., Port, A., Boicu, A. and sestacova, T. 2017. Molecular characterization of broomrape populations from Republic of Moldova using SSR markers. Helia 40:47-59. https://doi.org/10.1515/helia-2017-0003
  11. Felsenstein, J. 1993. Phylogeny inference package version 3.5c. URL https://csbf.stanford.edu/phylip/ [13 August 2021].
  12. Fernandez-Aparicio, M., Reboud, X. and Gibot-Leclerc, S. 2016. Broomrape weeds. Underground mechanisms of parasitism and associated strategies for their control: a review. Front. Plant Sci. 7:135. https://doi.org/10.3389/fpls.2016.00135
  13. Gagne, G., Roeckel-Drevet, P., Grezes-Besset, B., Shindrova, P., Ivanov, P., Grand-Ravel, C., Vear, F., de Labrouhe, D. T., Charmet, G. and Nicolas, P. 1998. Study of the variability and evolution of Orobanche cumana populations infesting sunflower in different European countries. Theor. Appl. Genet. 96:1216-1222. https://doi.org/10.1007/s001220050859
  14. Huson, D. H. and Bryant, D. 2006. Application of phylogenetic networks in evolutionary studies. Mol. Biol. Evol. 23:254-267. https://doi.org/10.1093/molbev/msj030
  15. Jebri, M., Ben Khalifa, M., Fakhfakh, H., Perez-Vich, B. and Velasco, L. 2017. Genetic diversity and race composition of sunflower broomrape populations from Tunisia. Phytopathol. Mediterr. 56:421-430.
  16. Joel, D. M., Benharrat, H., Portnoy, V. H. and Thalouarn, P. 1998. Molecular markers for Orobanche species: new approaches and their potential uses. In: Current Problems of Orobanche Researchers. Proceedings of the 4th International Workshop on Orobanche Research, eds. by K. Wegmann, L. J. Musselman and D. M. Joel, pp. 115-124. Institute for Wheat and Sunflower Dobroudja, Albena, Bulgaria.
  17. Katzir, N., Portnoy, V., Tzuri, G., Joel, D. M. and Castejon-Munoz, M. 1996. Use of random amplified polymorphic DNA (RAPD) markers in the study of the parasitic weed Orobanche. Theor. Appl. Genet. 93:367-372. https://doi.org/10.1007/bf00223178
  18. Malek, J., del Moral, L., Fernandez-Escobar, J., Perez-Vich, B. and Velasco, L. 2017. Racial characterization and genetic diversity of sunflower broomrape populations from northern Spain. Phytopathol. Mediterr. 56:70-76.
  19. Manen, J.-F., Habashi, C., Jeanmonod, D., Park, J.-M. and Schneeweiss, G. M. 2004. Phylogeny and intraspecific variability of holoparasitic Orobanche (Orobanchaceae) inferred from plastid rbcL sequences. Mol. Phylogenet. Evol. 33:482-500. https://doi.org/10.1016/j.ympev.2004.06.010
  20. Martin-Sanz, A., Malek, J., Fernandez-Martinez, J. M., PerezVich, B. and Velasco, L. 2016. Increased virulence in sunflower broomrape (Orobanche cumana Wallr.) populations from southern Spain is associated with greater genetic diversity. Front. Plant Sci. 7:589.
  21. Masirevic, S. and Medic-Pap, P. 2009. Broomrape in Serbia from its occurence till today. Helia 32:91-100. https://doi.org/10.2298/HEL0951091M
  22. Molinero-Ruiz, L., Garcia-Carneros, A. B., Collado-Romero, M., Raranciuc, S., Dominguez, J. and Melero-Vara, J. M. 2014. Pathogenic and molecular diversity in highly virulent populations of the parasitic weed Orobanche cumana (sunflower broomrape) from Europe. Weed Res. 54:87-96. https://doi.org/10.1111/wre.12056
  23. Moretti, M., Saracchi, M. and Farina, G. 2004. Morphological, physiological and genetic diversity within a small population of Cercospora beticola Sacc. Ann. Microbiol. 54:129-150.
  24. Nei, M. and Li, W. H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. U. S. A. 76:5269-5273. https://doi.org/10.1073/pnas.76.10.5269
  25. Paran, I., Gidoni, D. and Jacobsohn, R. 1997. Variation between and within broomrape (Orobanche) species revealed by RAPD markers. Heredity 78:68-74. https://doi.org/10.1038/sj.hdy.6881070
  26. Parker, C. and Riches, C. R. 1993. Parasitic weeds of the world: biology and controls. CAB International, Wallingford, UK. 332 pp.
  27. Pineda-Martos, R., Velasco, L., Fernandez-Escobar, J., Fernandez-Martinez, J. M. and Perez-Vich, B. 2013. Genetic diversity of Orobanche cumana populations from Spain assessed using SSR markers. Weed Res. 53:279-289. https://doi.org/10.1111/wre.12022
  28. Pineda-Martos, R., Velasco, L. and Perez-Vich, B. 2014. Identification, characterisation and discriminatory power of microsatellite markers in the parasitic weed Orobanche cumana. Weed Res. 54:120-132. https://doi.org/10.1111/wre.12062
  29. Roman, B. and Rubiales, D. 1999. Molecular analysis of Orobanche crenata populations from southern Spain. In: Resistance to Orobanche: the state of the art, eds. by J. I. Cubero, M. T. Moreno, D. Rubiales and J. Sillero, pp. 99-108. Junta de Andalucia, Sevilla, Spain.
  30. Satovic, Z., Joel, D. M., Rubiales, D., Cubero, J. I. and Roman, B. 2009. Population genetics in weedy species of Orobanche. Australas. Plant Pathol. 38:228-234. https://doi.org/10.1071/AP08100
  31. Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729. https://doi.org/10.1093/molbev/mst197
  32. Yang, C., Fu, F., Zhang, N., Wang, J., Hu, L., Islam, F., Bai, Q., Yun, X. and Zhou, W. 2020. Transcriptional profiling of underground interaction of two contrasting sunflower cultivars with the root parasitic weed Orobanche cumana. Plant Soil 450:303-321. https://doi.org/10.1007/s11104-020-04495-3
  33. Young, N. D., Steiner, K. E. and dePamphilis, C. W. 1999. The evolution of parasitism in Scrophulariaceae/Orobanchaceae: plastid gene sequences refute an evolutionary transition series. Ann. Missouri Bot. Gard. 86:876-893. https://doi.org/10.2307/2666173
  34. Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535. https://doi.org/10.1093/nar/18.22.6531
  35. Wolfe, A. D. and dePamphilis, C. W. 1997. Alternate paths of evolution for the photosynthetic gene rbcL in four nonphotosynthetic species of Orobanche. Plant Mol. Biol. 33:965-977. https://doi.org/10.1023/A:1005739223993
  36. Wolfe, A. D. and dePamphilis, C. W. 1998. The effect of relaxed functional constraints on the photosynthetic gene rbcL in photosynthetic and nonphotosynthetic parasitic plants. Mol. Biol. Evol. 15:1243-1258. https://doi.org/10.1093/oxfordjournals.molbev.a025853