• Toxicological Research
• /
• v.16 no.1
• /
• pp.39-45
• /
• 2000

This study was carried out to evaluate antitoxic effects Palsun Brewing Water against cadmium by colorimetric methods. The antitoxic activity of Palsun Brewing Water in NIH-3T3 fibroblasts was evaluated by MTT ({3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-2H-tetrazoliumbromide} and SRB (sulforhodamine B protein) assays. The light microcopic study was carried out to observe morphological changes of the treated cells. These results were obtained as follows: The concentration of 10-2 mg/ml of Palsun Brewing Water was shown significant antitoxic activity against E. coli endotoxin and Salmonella endotoxin. The number of NIH-3T3 fibroblasts were antitoxin and tend to regenerate. These results suggest that Palsun Brewing Water retains a potential antitoxic activity.

• Toxicological Research
• /
• v.8 no.1
• /
• pp.119-129
• /
• 1992

Present study was carried out to investigate the cytotoxicity of cadmium, chromium and mercury on cultured rat fibroblasts. The colorimetric assays of neutral red (NR) and tetrazolium MTT, the measurement of total content of protein and electron microscopic studies were performed on the fibroblasts cultured in the media containing various concentrations of cadmium, chromium and mercury. The results are as follows' 1. In cadmium-treated group, the NR and MTT values were dose-dependent increase. The NR90, NR50, MTT90 and MTT50 values of cadmium were 0.2mM, 19.5mM 1.0mM and 60.0mM, respectively.

• Journal of Veterinary Clinics
• /
• v.25 no.3
• /
• pp.200-201
• /
• 2008

A case of cutaneous fibroma was diagnosed in the skin of the right hindlimb of a Korean indigenous cattle in Kyungpook province, Korea. Grossly, the protruding skin nodules consisted of a solitary mass $(1.5{\times}1.5{\sim}3.0{\times}3.0cm)$ above large grapelike warty nodules $(11.0{\times}11.0cm)$ on the leg. These masses were firm and rubbery, and the cut surface was gray to white. Histopathologically, the benign neoplastic nodules consisted of spindle-type fibroblasts with collagen. Melanoma, sarcoma, and fibropapilloma were excluded because there was no sign of melanin, muscle type cells, or epidermal proliferation. To our knowledge, this is the first report of a cutaneous fibroma in a Korean indigenous cattle in Korea.

• BMB Reports
• /
• v.36 no.3
• /
• pp.265-268
• /
• 2003

Retinoic acid (RA) can transform the Golgi apparatus (GA) into a diffuse vacuolar aggregate and increase the toxicity of some immunotoxins that enter into cells by receptor-mediated endocytosis. An ultramorphological study of the RA-induced GA disruption was performed on F2000 fibroblasts. Cultures were treated with 0.11 to $30\;{\mu}M$ RA for 7 - 180 min. The endocytosis of Limax flavus agglutinin-peroxidase conjugate (LFA), and the interactions between a phorbol ester (PMA) and RA concerning GA disruption, were examined. Exposure to $0.33\;{\mu}M$ RA for 20 min transformed the GA into vacuolar aggregate. These vacuoles were not involved in endocytosis since they remained unstained after endocytosis of LFA. However, the lysosomes were involved in endocytosis, as they were strongly stained. Therefore, a RA-induced shift towards lysosomal routing of the entered LFA was presumed. Exposure to PMA made cells resistant to the Golgi-disturbing effects of RA, indicating that protein kinase C plays an important role in this process.

• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.337.3-338
• /
• 2002

UV-B irradiation increases the synthesis of matrixmetalloproteinase-1 (MMP-1) that degrades skin collagen in human skin. In this work, we investigated the photoprotective effect of decursinol angelate (DEA) extracted Irom Angelica gigas on human skin libroblasts. DEA inhibited UVB-induced MMP-1 induction, which was conlirmed by western blot and ELISA. We examined upstream signal transduction pathway and the action mechanism of DEA on UVB induction of MMp in human skin fibroblasts. (omitted)

• International Journal of Oral Biology
• /
• v.30 no.1
• /
• pp.31-37
• /
• 2005

Treponema maltophilum, a Group IV oral spirochete, is associated with periodontitis and endodontic infections. In this study we analyzed the functional role of the major surface protein of this organism (MspA) in human gingival fibroblasts (HGFs). The full-length gene encoding MspA was cloned and expressed in Escherichia coli by using the expression vector pQE-30. The recombinant protein (rMspA) was purified by affinity chromatography with nickel-nitrilotriacetic acid agarose and possible contamination of E. coli endotoxin in rMspA was removed by using polymyxin B-agarose. rMspA significantly induced the expression of pro inflammatory cytokines like IL-6 and IL-8 and intercellular adhesion molecule (ICAM)-1 in HGFs, when analyzed by reverse transcription-PCR, flow cytometry, and enzyme-linked immunosorbent assay. Our results indicate that MspA of T. maltophilum may play an important role in amplifying the local immune response by upregulating the expression of proinflammatory cytokines and ICAM-1.

• Toxicological Research
• /
• v.7 no.1
• /
• pp.13-20
• /
• 1991

To investigate the cytotoxicity and genotoxicity of the DNA alkylating agnet, mitomycin C and the antimetabolite, 5-Fluorouracil (5-FU) in cultured rat fibroblasts, the colorimetric assay of netural red (NR) for cytotoxicity and for genotoxicity, sister chromatid exchange (SCE) assay and the measurement of the rate of DNA synthesis were performed in cells cultured in media containing various concentrations of mitomycin C and 5-FU. The uptake ability of neutral red decreased does-dependently. NR90 and NR50 values of mitomycin C were 1.49 nM and 6.87mM and 5-FU were 38.4mM AND 284.4Mm respectively.

• Toxicological Research
• /
• v.26 no.1
• /
• pp.61-66
• /
• 2010

Retinoids have many beneficial effects on dermatological applications. But, retinoids cause skin irritation. In this study, the safety of retinoids was clarified via both primary skin irritation test in rabbits and sensitization study using an integrated model for the differentiation of chemical-induced allergic and irritant skin reaction (IMDS), an alternative method to sensitization test. The effects of retinoids on the change of ultraviolet A (UVA)-induced matrix metalloproteinase-1 (MMP-1) in human skin fibroblasts and the modulation of type-1 pN collagen synthesis in hairless mice were examined to clarify the anti-wrinkle effects. Alltrans retinol (t-ROL) and its derivative, all-trans retinoic acid (t-RA), showed mild skin irritation but did not induce the sensitization. t-ROL and t-RA exerted anti-wrinkle effects by inhibiting the UVA-induced MMP-1 in human skin fibroblasts and increasing the type-1 pN collagen synthesis in hairless mice. These findings suggest that retinoids do not induce the allergy, and show anti-wrinkle effects by decreasing MMP-1 activation and increasing collagen synthesis.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.25 no.3
• /
• pp.528-532
• /
• 2011

This study was designed to investigate the collagen metabolism and tyrosinase activity of Meretrix extracts (ME). The effect of ME on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of ME was measured as well. Type I procollagen production was recovered by ME in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by ME. The tyrosinase activity and L-DOPA oxidation were significantly reduced as well. However, the effects on tyrosinase activity and L-DOPA oxidation were not powerful enough to be used as whitening agents. ME showed the anti-wrinkle effects and some whitening effects in vitro. These results suggest that ME may be a useful drug as an anti-wrinkle treatments.

• Proceedings of the KSAR Conference
• /
• 2001.10a
• /
• pp.60-61
• /
• 2001

To produce reconstituted rabbit embryos with fetal fibroblasts, the present study was evaluated the efficiencies of the activation conditions as assessments of subsequent development and chromosome in the embryos. New Zealand White rabbits were used throughout the study. Fetal fibroblasts collected from 22-d of fetuses were cultured in DMEM＋10% FBS in 5% CO₂ in air. The culture was maintained for 10 passages. In every passage half of cell suspension were kept in frozen. (omitted)