Browse > Article

Functional Characterization of the Major Surface Protein of Treponema maltophilum in Human Gingival Fibroblasts  

Lee, Sung-Hoon (Department of Oromaxillofacial Infection and Immunity College of Dentistry, Seoul National University)
Choi, Bong-Kyu (Department of Oromaxillofacial Infection and Immunity College of Dentistry, Seoul National University)
Publication Information
International Journal of Oral Biology / v.30, no.1, 2005 , pp. 31-37 More about this Journal
Abstract
Treponema maltophilum, a Group IV oral spirochete, is associated with periodontitis and endodontic infections. In this study we analyzed the functional role of the major surface protein of this organism (MspA) in human gingival fibroblasts (HGFs). The full-length gene encoding MspA was cloned and expressed in Escherichia coli by using the expression vector pQE-30. The recombinant protein (rMspA) was purified by affinity chromatography with nickel-nitrilotriacetic acid agarose and possible contamination of E. coli endotoxin in rMspA was removed by using polymyxin B-agarose. rMspA significantly induced the expression of pro inflammatory cytokines like IL-6 and IL-8 and intercellular adhesion molecule (ICAM)-1 in HGFs, when analyzed by reverse transcription-PCR, flow cytometry, and enzyme-linked immunosorbent assay. Our results indicate that MspA of T. maltophilum may play an important role in amplifying the local immune response by upregulating the expression of proinflammatory cytokines and ICAM-1.
Keywords
Treponema maltophilum; Major surface protein; Proinflammatory cytokines; ICAM-l;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Lee HJ, Kang IK, Chung CP, and Choi SM: The subgingival microflora and gingival crevicular fluid cytokines in refractory periodontitis. J Clin Periodontol. 22: 885-890, 1995   DOI   ScienceOn
2 Masaka T, Hayashi J, and Ishikawa I: Soluble CD14-dependent intercellular adhesion molecular-1 induction by Porphyromonas gingivalis lipopolysaccharide in human gingival fibroblasts. J Periodontol. 70: 772-8, 1999   DOI   PUBMED
3 Page RC, Offenbacher S, Schroeder HE, Seymour GJ, and Komman KS: Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions. Periodontol 2000. 14: 216-48, 1997   DOI   PUBMED   ScienceOn
4 Park KK, Heuner K, Gobel UB, Yoo YJ, Kim CK, and Choi BK: Cloning and characterization of a major surface protein (MspTL) of Treponema lecithinolyticum associated with rapidly progressive periodontitis. FEMS Microbiol Lett. 207: 185-192, 2002   DOI   ScienceOn
5 Sugiyama A, Uehara A, Iki K, Matsushita K, Nakamura R, Ogawa T, Sugawara S, and Takada H: Activation of human gingival epithelial cells by cell-surface components of black-pigmented bacteria: augmentation of production of interleukin-8, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor and expression of intercellular adhesion molecule 1. J Med Microbiol. 51: 27-33, 2002   DOI
6 Wyss C, Choi BK, Schupbach P, Moter A, Heuner K, Guggenhein B, and Gobel UB: Treponema lecithinolyticum sp. Nov., a small saccharolytic spirochete with phospholipase a and C activities associated with periodontal diseases. lnt J Syst Bacteriol.49: 1329-1339, 1999   DOI   ScienceOn
7 Yamada M, Nakae H, Yumoto H, Shinohara C, Ebisu S, and Matsuo T: N-acetyl-D-galactosamine specific lectin of Eikenella corrodens induces intercellular adhesion molecule-1 (lCAM-1) production by human oral epithelial cells. J Med Microbiol. 51: 1080-1089, 2002   DOI
8 Komman KS, Page RC, and Tonetti MS: The host response to the microbial challenge in periodontitis: assembling the players. Periodontol 2000.14: 33-53, 1997   DOI   PUBMED   ScienceOn
9 Dennison DK and Van Dyke TE: The acute inflammatory response and the role of phagocytic cells in periodontal health and disease. Periodontol 2000.14: 54-78, 1997   DOI   PUBMED
10 Hayashi J, Saito I, Ishikawa I, and Miyasaka N: Effects of cytokines and periodontopathic bacteria on the leukocyte function-associated antigen 1/intercellular adhesion molecule 1 pathway in gingival fibroblasts in adult periodontitis. Infect Immun. 62: 5205-5212, 1994   PUBMED
11 Fenno JC, Hannam PM, Leung WK, Tamura M, Ditto VJ, and McBride BC. Cytopathic effects of the major surface protein and the chymotrypsinlike protease of Treponema denticola. Infect Immun. 66: 1869-1877, 1998a
12 Moter A, Hoenig C, Choi BK, Riep B, and Gobel UB: Molecular epidemiology of oral treponemes associated with periodontal disease. J Clin Microbiol. 36: 1399-1403, 1998
13 Graves DT: The potential role of chemokines and inflammatory cytokines in periodontal disease progression. Clin Infect Dis. 28: 82-90, 1999   DOI   PUBMED   ScienceOn
14 Wang Q, Ko KS, Kapus A, McCulloch CA, and Ellen R: A spirochete surface protein uncouples store-operated calcium channels in fibroblasts: a novel cytotoxic mechanism. J Biol Chem. 276: 23056-23064, 2001   DOI   ScienceOn
15 Yuko M, Hiroyuki T, Shigeo K, and Shigemasa H: Porphyromonas gingivalis lipopolysaccharide-stimulated bone resorption via CD14 is inhibited by broad-spectrum antibiotics. Infect Immun. 65: 3513-3519, 1997
16 Heuner K, Choi BK, Schade R, Moter A, Otto A, and Gobel UB: Cloning and characterization of it gene(mspA) encoding the major sheath protein of Treponema maltophilum ATCC 51939. J Bacteriol. 181: 1025-1029, 1999
17 Roodman GD: Role of cytokines in the regulation of bone resorption. Calcif Tissue Int. 53: 94-98, 1992   DOI   PUBMED
18 Choi BK, Paster BJ, Dewhirst FE, and Gobel UB: Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. Infect Immun. 62: 1889-1895, 1994
19 Lee SH, Kim KK, and Choi BK: Upregulation of intercellular adhesion molecule 1 and pro inflammatory cytokines by the major surface proteins of Treponema maltophilum and Treponema lecithinolyticum, the phylogenetic group IV oral spirochetes associated with periodontitis and endodontic infections. Infect Immun. 73: 268-276, 2005   DOI   ScienceOn
20 Wyss C, Choi BK, Schupbach P, Guggenheim B, Gobel UB. Treponema maltophi/um sp. nov., a small oral spirochete isolated from human periodontal lesions. lnt J Syst Bacteriol. 46: 745-752, 1996   DOI   ScienceOn
21 Fenno JC and McBride BC: Virulence factors of oral treponemes. Anaerobe 4: 1-17, 1998b   DOI   ScienceOn
22 Dewhirst FE, Tamer MA, Ericson RE, Lau CN, Levanos VA, Boches SK, Galvin JL, and Pater BJ: The diversity of periodontal spirochetes by l6S rRNA analysis. Oral Microbiol Immunol. 15: 196-202, 2000   DOI   ScienceOn
23 Kim CK, Yoo YJ, Kim YH, and Choi BK: Functional characterization of the major outer membrane protein (MspTL) of Treponema lecithinolyticum. J Bacteriol Virol. 32: 47-54, 2002
24 Schwartz Z, Goultschin J, Dean DD, Boyan BD. Mechanisms of alveolar bone destruction in periodontitis. Periodontol 2000.14: 158-172, 1997   DOI   PUBMED   ScienceOn
25 Seshadri R, Myers GS, Tettelin H, Eisen JA, Heidelberg JF, Dodson RJ, Davidsen TM, DeBoy RT, Fouts DE, Haft DH, Selengut J, Ren Q, Brinkac LM, Madupu R, Kolonay J, Durkin SA, Daugherty SC, Shetty J, Shvartsbeyn A, Gebregeorgis E, Geer K, Tsegaye G, Malek J, Ayodeji B, Shatsman S, McLeod MP, Smajs D, Howell JK, Pal S, Amin A, Vashisth P, McNeill TZ, Xiang Q, Sodergren E, Baca E, Weinstock GM, Norris SJ, Fraser CM, and Paulsen IT: Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes. PNAS. 101: 5646-5151, 2004   DOI   ScienceOn
26 Irwin CR and Myrillas TT: The role of IL-6 in the pathogenesis of periodontal disease. Oral Dis. 4: 43-47, 1998   DOI   ScienceOn