Browse > Article

Effects of Meretrix Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity  

Leem, Kang-Hyun (College of Oriental Medicine, Semyung University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.25, no.3, 2011 , pp. 528-532 More about this Journal
Abstract
This study was designed to investigate the collagen metabolism and tyrosinase activity of Meretrix extracts (ME). The effect of ME on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of ME was measured as well. Type I procollagen production was recovered by ME in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by ME. The tyrosinase activity and L-DOPA oxidation were significantly reduced as well. However, the effects on tyrosinase activity and L-DOPA oxidation were not powerful enough to be used as whitening agents. ME showed the anti-wrinkle effects and some whitening effects in vitro. These results suggest that ME may be a useful drug as an anti-wrinkle treatments.
Keywords
Meretrix; Meretrix extracts; type I procollagen; collagenase; tyrosinase;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Muller, W.E.G. The origin of metazoan complexity: Porifera as integrated animals. Integr Comp Biol 43(1):3-10, 2003.   DOI   ScienceOn
2 Di Lullo, G.A., Sweeney, S.M., Korkko, J., Ala-Kokko, L., San Antonio, J.D. Mapping the ligand-binding sites and disease-associated mutations on the most abundant protein in the human, type I collagen. J Biol Chem 277(6):4223-4231, 2002.   DOI
3 Sikorski, Z.E. Chemical and functional properties of food proteins. Boca Raton, CRC Press, p 242, 2005.
4 Wei, X., Liu, Y., Xiao, J., Wang, Y. Protective effects of tea polysaccharides and polyphenols on skin. J Agric Food Chem 57(17):7757-7762, 2009.   DOI   ScienceOn
5 Moon, H.J., Lee, S.H., Ku, M.J., Yu, B.C., Jeon, M.J., Jeong, S.H., Stonik, V.A., Zvyagintseva, T.N., Ermakova, S.P., Lee, Y.H. Fucoidan inhibits UVB-induced MMP-1 promoter expression and down regulation of type I procollagen synthesis in human skin fibroblasts. Eur J Dermatol 19(2):129-134, 2009.
6 Vanni, A., Gastaldi, D., Giunata, G. Kinetic investigations on the double enzyme activity of the tyrosinase mushroom. Ann Chim 80: 35-60, 1990.
7 Joshi, P.C., Carraro, C., Pathak, M.A. Involvement of reactive oxygen species in the oxidation of tyrosine and dopa to melanin and in skin tanning. Biochem Biophys Res Commun 142(1):265-274, 1987.   DOI   ScienceOn
8 The Boncho-hak textbook committee. Boncho-Hak. Seoul, Young-Lim Press, p 724, 2007.
9 Chen, J.L., Chen, T.T. Chinese medical herbology and pharmacology. CA, Art of Medicine Press. p 713, 2004.
10 Yaar, M., Gilchrest, B.A. Cellular and molecular mechanisms of cutaneous aging. J Dermatol Surg Oncol 16(10):915-922, 1990.   DOI
11 Gelse, K., Poschl, E., Aigner, T. Collagens-structure, function, and biosynthesis. Adv Drug Deliv Rev 55(12):1531-1546, 2003.   DOI   ScienceOn
12 Kim, Y.H., Chung, C.B., Kim, J.G., Ko, K.I., Park, S.H., Kim, J.H., Eom, S.Y., Kim, Y.S., Hwang, Y.I., Kim, K.H. Anti-wrinkle activity of ziyuglycoside I isolated from a Sanguisorba officinalis root extract and its application as a cosmeceutical ingredient. Biosci Biotechnol Biochem 72(2):303-311, 2008.   DOI   ScienceOn
13 Fisher, G.J., Datta, S.C., Talwar, H.S., Wang, Z.Q., Varani, J., Kang, S., Voorhees, J.J. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379(6563):335-339, 1996.   DOI   ScienceOn
14 Shin, J.Y., Hur, W., Wang, J.S., Jang, J.W., Kim, C.W., Bae, S.H., Jang, S.K., Yang, S.H., Sung, Y.C., Kwon, O.J., Yoon, S.K. HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-beta1. Exp Mol Med 37(2):138-145, 2005.   DOI   ScienceOn
15 Lei, T.C., Virador, V.M., Vieira, W.D., Hearing, V.J. A melanocyte-keratinocyte coculture model to assess regulators of pigmentation in vitro. Anal Biochem 305(2):260-268, 2002.   DOI   ScienceOn