Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2001.10a
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- Pages.60-61
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- 2001
Development of Reconstituted Embryos with Fetal Fibroblast Cells in Rabbit
- J. G. Yoo (College of Veterinary Medicine, Gyeongsang National University) ;
- S. R. Cho (Division of Applied Life Science, Gyeongsang National Universit) ;
- Lee, S. L. (College of Veterinary Medicine, Gyeongsang National Universit) ;
- J. M. Hwang (College of Veterinary Medicine, Gyeongsang National Universit) ;
- J. S. Bhak (College of Veterinary Medicine, Gyeongsang National Universit) ;
- E. H. Yea (College of Veterinary Medicine, Gyeongsang National Universit) ;
- Park, G. J. (College of Veterinary Medicine, Gyeongsang National Universit) ;
- Lee, H. J. (College of Veterinary Medicine, Gyeongsang National Universit) ;
- S. Y. Choe (College of Veterinary Medicine, Gyeongsang National University)
- Published : 2001.10.01
Abstract
To produce reconstituted rabbit embryos with fetal fibroblasts, the present study was evaluated the efficiencies of the activation conditions as assessments of subsequent development and chromosome in the embryos. New Zealand White rabbits were used throughout the study. Fetal fibroblasts collected from 22-d of fetuses were cultured in DMEM＋10% FBS in 5% CO₂ in air. The culture was maintained for 10 passages. In every passage half of cell suspension were kept in frozen. (omitted)
Keywords
- cycloheximide;
- 6-dimethylaminopurin;
- Na-pyrophosphate;
- fetal fibroblast;
- rabbit;
- reconstituted embryo