• Title/Summary/Keyword: fiber fragmentation

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Postmorten Changes in the Myofibrillar Proteins of Alkaline and Acid Rigor Rabbit Muscle during Storage (사후강직형태에 따른 근원섬유 단백질의 이화학적 성질변화)

  • Kim, Dae-Gon;Sung, Sam-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.6
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    • pp.712-718
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    • 1992
  • This experiment was carried out to investigate some physico-chemical properties of myofibrillar proteins isolated from alkaline and acid rigor muscles of rabbit. The degree of fragmentation was about 0.35 at 3 days of postmortem in acid rigor muscle fiber, whereas it was only 0.3 at 7 days of postmortem in alkaline rigormuscle fiber. $Mg^{2+}$-activated ATPase activities of actomyosin were increased to the highest level at 1st day in acid rigor and at 3rd day in alkaline rigor muscle fiber. $Ca^{2+}$-activated ATPase activities of actomyosin were slightly increased at postmortem. $Mg^{2+}$-, and $Ca^{2+}$-activated ATPase activities were higher in alkaline rigor muscle than those in acid rigor muscle at postmortem. Solubility of actomyosin increased with postmortem but no differences were observed in rigor types. SDS-PAGE(sodium dodecyl sulfate polyacrylamide gel electrophoresis) band patterns of myofibrils did not show significant differences between rigor types, but newly 30.000 dalton proteins were appeared at 3 day postmortem in both rigor types.

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Anti-tumor Activity of Saussurea laniceps against Pancreas Adenocarcinoma

  • Lee, Keyong Ho;Kim, Byeong- Soo;Rhee, Ki-Hyeong
    • Natural Product Sciences
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    • v.23 no.4
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    • pp.281-285
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    • 2017
  • The purpose of this study was to confirm the anti-tumor activity of an ethanol extract of Saussurea laniceps against pancreatic tumor and to isolate the active compound from S. laniceps extract. Treatment with S. laniceps extract and hispidulin inhibited proliferation of pancreatic cell lines, such as Capan-1, Capan-2, Panc-1 and S2-013 in a dose-dependent manner using the hollow fiber assay. Hispidulin showed typical hallmarks of apoptotic cell death a significant anti-tumor activity on Capan-2 cells at a dose of 100 mg/kg and 200 mg/kg. S. laniceps has potential cytotoxic and apoptotic effects on human pancreatic carcinoma cells. Its mechanism of action might be associated with the apoptotic cell death through DNA fragmentation.

Preparation and Analysis of Activated Carbon Fiber from PAN Precursor(I) (PAN 선구체로부터 활성 탄소섬유의 생산과 분석(I))

  • 김진홍;이화선;박병기;정경락;김공주
    • Textile Coloration and Finishing
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    • v.4 no.4
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    • pp.90-96
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    • 1992
  • In the PAN-based ACF manufacturing system stabilization step was improved with chemical treatment (preoxidation) in order to yield higher carbon content and to avoid excessive fragmentation during carbonization and activation process. The optimal condition of preoxidation was at 18$0^{\circ}C$ for 4 minutes in sodium glyceroxide in glycerine (concentration of NaOH was 0.02 meq/g). To investigate low temperature stabilization effect, preoxidized PAN fiber heated 22$0^{\circ}C$ to 26$0^{\circ}C$ as a function of treatment time and oxidative gas atmosphere, and analysed by infrared spectrum and TGA. As a results of IR and TGA, it was clear that impregnated[preoxidative] PAN had 14% higher residual than untreated PAN at 100$0^{\circ}C$ and the optimal condition of stabilization was at 26$0^{\circ}C$ for 3.5 hours within $N_2$ atmosphere.

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CLINICAL EFFORTS FOR TECHNICAL IMPROVEMENT IN TOOTH FRAGMENTATION (초기 영구 전치 파절시의 치아 재부착술의 임상 증례)

  • Choi, Sung-Chul;Lee, Keung-Ho;Choi, Yeong-Chul
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.1
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    • pp.125-131
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    • 2002
  • When a tooth is fracture with the pulp exposure, and if a fragment is large enough to save, reattachment of the fragment would be a choice of treatment and reattachment of the fragment provides several advantages over other forms of dental restoration following crown fracture. For the purpose of tooth reattachment, it is important to preserve the sound enamel around fracture area. For young patients, tooth reattachment has more advantages in the sense that they recover faster and that it enables other treatments at the same time. Through the continuous advancement in adhesion technology and the effort for aestheticism, various reattachment methods have been practiced and more efforts are considered necessary. The presented cases are that we had practiced in our department in Kyung Hee Medical center, I may address that fragmentation has been successfully done with aesthetically fine results, and no pathologic changes were found in short term follow-ups.

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Effect of the surface modification using MWCNTs with different L/D by two different methods of deposition on the IFSS of single carbon fiber-epoxy resin composite

  • Herrera-Sosa, Minerva L.;Valadez-Gonzalez, Alex;Vazquez-Torres, Humberto;Mani-Gonzalez, Pierre G.;Herrera-Franco, Pedro J.
    • Carbon letters
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    • v.24
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    • pp.18-27
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    • 2017
  • Multiwall carbon nanotubes (MWCNT) with two different (L/D) aspect ratios ($7{\pm}2{\mu}m/140{\pm}30nm$ and $0.5-2{\mu}m/8-15nm$) were surface treated using nitric acid ($HNO_3$) and polyethyleneimine (PEI) prior to their deposition on carbon fibers (CF). Before the hierarchical reinforcement with CF-MWCNT, the CFs were treated with 3-glycidoxypropyltrime-thoxysilane, a coupling agent (Z6040) and with poly(amidoamine) (PAMAM) a dendrimer containing an ethylenediamine core and amine surface groups. The MWCNT were deposited on the CF using two methods, by electrostatic attraction and by chemical reactions. The changes in the CF surface morphology after the MWCNT deposition were analyzed using SEM, which revealed a higher density and uniform coverage for the PAMAM-treated CF and the short MWCNTs. The interfacial adhesion of the composite materials was evaluated using the single fiber fragmentation technique. The results indicated an improvement in the interfacial shear strength with the addition of the short-MWCNTs treated with acid solutions and grafted onto the surface of the CF fiber using electrostatic attraction.

Nondestructive Interfacial Evaluation and fiber fracture Source Location of Single-Fiber/Epoxy Composite using Micromechanical Technique and Acoustic Emission (음향방출과 미세역학적시험법을 이용한 단일섬유강화 에폭시 복합재료의 비파지적 섬유파단 위치표정 및 계면물성 평가)

  • Park, Joung-Man;Kong, Jin-Woo;Kim, Dae-Sik;Yoon, Dong-Jin
    • Journal of the Korean Society for Nondestructive Testing
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    • v.23 no.5
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    • pp.418-428
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    • 2003
  • Fiber fracture is one of the dominant failure phenomena affecting the total mechanical Performance of the composites. Fiber fracture locations were measured through the conventional optical microscope and the nondestructive acoustic emission (AE) technique and then were compared together as a function of the epoxy matrix modulus and the fiber surface treatment by the electrodeposition method (ED). Interfacial shear strength (IFSS) was measured using tensile fragmentation test in combination of AE method. ED treatment of the fiber surface enlarged the number of fiber fracture locations in comparison to the untreated case. The number of fiber fracture events measured by the AE method was less than optically obtained one. However, fiber fracture locations determined by AE detection corresponded with those by optical observation with small errors. The source location of fiber breaks by AE analysis could be a nondestructive, valuable method to measure interfacial shear strength (IFSS) of matrix in non-, semi- and/or transparent polymer composites.

Histomorphological changes in the common carotid artery of the male rat in induced hypogonadism

  • Cheruiyot, Isaac;Olabu, Beda;Kamau, Martin;Ongeti, Kevin;Mandela, Pamela
    • Anatomy and Cell Biology
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    • v.51 no.4
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    • pp.284-291
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    • 2018
  • The role of androgens in the development of cardiovascular diseases remains controversial. The current study therefore sought to determine the changes in the histomorphology of the common carotid artery of the male rat in orchidectomy-induced hypogonadism. Twenty-two Rattus norvegicus male rats aged 2 months were used. The rats were randomly assigned into baseline (n=4), experimental (n=9), and control (n=9) groups. Hypogonadism was surgically induced in the experimental group by bilateral orchiectomy under local anesthesia. At experiment weeks 3, 6, and 9, three rats from each group (experimental and control) were euthanized, their common carotid artery harvested, and routine processing was done for paraffin embedding, sectioning, and staining. The photomicrographs were taken using a digital photomicroscope for morphometric analysis. Orchidectomy resulted in the development of vascular fibrosis, with a significant increase in collagen fiber density and decrease in smooth muscle and elastic fiber density. Moreover, there was development of intimal hyperplasia, with fragmentation of medial elastic lamellae in the common carotid artery of the castrated rats. Orchidectomy induces adverse changes in structure of the common carotid artery of the male rat. These changes may impair vascular function, therefore constituting a possible structural basis for the higher incidences of cardiovascular diseases observed in hypogonadism.

Cadmium Toxicity on the Survival Rate and Activity of the Equilateral Venus, Gomphina veneriformis (Bivalvia: Veneridae) (대복 (Gomphina veneriformis)의 생존 및 운동성에 미치는 카드뮴 (cd)의 독성)

  • PARK Jung Jun;LEE Jung Sick
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.36 no.5
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    • pp.463-468
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    • 2003
  • This study observed change of survival rate, activity and foot structure of the equilateral venus (Gomphina veneriformis) exposed to cadmium. Survival rate and activity of the clam exposed to cadmium was reduced with increase of exposure duration and concentration. Change of survival rate and activity was observed in the early exposure time (7 days) in the condition of above 1 77 mg/L and 0.88 mg/L, respectively. Activity reduction of the clam exposed to cadmium seems to be caused by epidermal layer deformation, muscle fiber fragmentation and muscular layer collapse of the foot.

Numerical Study on Columns Subjected to Blast Load Considering Compressive Behavior of Steel Fiber Reinforced Concrete (강섬유보강콘크리트의 압축거동 특성을 반영한 기둥의 내폭해석 )

  • Jae-Min Kim;Sang-Hoon Lee;Jae Hyun Kim;Kang Su Kim
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.27 no.5
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    • pp.105-112
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    • 2023
  • Steel fiber reinforced concrete (SFRC) exhibits enhanced strength and superior energy dissipation capacity compared to normal concrete, and it can also reduce crack propagation and fragmentation of concrete even when subjected to blast loads. In this study, the parameters defining failure surface and damage function of the K&C concrete nonlinear model were proposed to be applied for the properties of SFRC in LS-DYNA. Single element analysis has been conducted to validate the proposed parameters in the K&C model, which provided very close simulations on the compressive behavior of SFRC. In addition, blast analysis was performed on SFRC columns with different volume fractions of steel fibers, and the blast resistance of SFRC columns was quantitatively analyzed with Korea Occupational Safety & Health Agency (KOSHA) guidelines.

Effects of Exercise Intensity on Bcl-2, Bax, Caspase-3 Protein Level and DNA Fragmentation in Soleus and EDL Muscle on 60 wk SD Rats (운동강도의 차이가 60주령된 흰쥐의 Soleus와 EDL에서 Bcl-2, Bax, caspase-3와 DNA 절편화에 미치는 영향)

  • Lee, Ji-Yung;Kim, Yong-An
    • Journal of Life Science
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    • v.20 no.4
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    • pp.572-577
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    • 2010
  • The purpose of this study was to find out the Bcl-2 (B-cell leukemia/lymphoma-2), Bax, and caspase-3(cysteine-aspartic proteases-3) protein expression in soleus and EDL muscle according to treadmill exercise intensity in 60 week-old SD rats. The SD rats were randomly divided into four groups (n=10 in each group): control (CON), low intensity exercise (LE), moderate intensity exercise (ME), and high intensity exercise (HE). The exercise was given to the rats for 8 wk, 5 day/wk. The animals underwent treadmill exercise at intensities of 30 min at 8 m/min for the LE group, 15 min at 16 m/min for the ME group, and 9 min at 24 m/min for the HE group. The results were as follows: the expression of Bcl-2 protein was lowest in the HE group and the expression of Bax protein was highest in the HE group. The expression of caspase-3 (cleaved form) protein was observed in the HE group. For the different types of muscle fiber, Bcl-2 protein expression in the soleus muscle was decreased in all groups. Bax protein expression in the soleus muscle was increased in the HE group only. Bcl-2 protein expression in the EDL muscle was decreased in the HE group, and Bax protein expression in the EDL muscle was increased in the ME and HE groups. Consequently, the protein expression related to the aged rats shows a difference according to the intensity of exercise. In addition, caspase-3 protein expression appeared in the HE group; however, in all amounts of intensity, DNA fragmentation was not observed. Therefore, apoptosis on skeletal muscles of aged mice can be intervened with optimal exercise. On the other hand, high intensity exercise can potentially accelerate the apoptosis of muscle fiber in aged rats.