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http://dx.doi.org/10.5352/JLS.2010.20.4.572

Effects of Exercise Intensity on Bcl-2, Bax, Caspase-3 Protein Level and DNA Fragmentation in Soleus and EDL Muscle on 60 wk SD Rats  

Lee, Ji-Yung (Department of Physical Education, Dankook University)
Kim, Yong-An (Institute of Biotechnology, Chungnam Nat'l University)
Publication Information
Journal of Life Science / v.20, no.4, 2010 , pp. 572-577 More about this Journal
Abstract
The purpose of this study was to find out the Bcl-2 (B-cell leukemia/lymphoma-2), Bax, and caspase-3(cysteine-aspartic proteases-3) protein expression in soleus and EDL muscle according to treadmill exercise intensity in 60 week-old SD rats. The SD rats were randomly divided into four groups (n=10 in each group): control (CON), low intensity exercise (LE), moderate intensity exercise (ME), and high intensity exercise (HE). The exercise was given to the rats for 8 wk, 5 day/wk. The animals underwent treadmill exercise at intensities of 30 min at 8 m/min for the LE group, 15 min at 16 m/min for the ME group, and 9 min at 24 m/min for the HE group. The results were as follows: the expression of Bcl-2 protein was lowest in the HE group and the expression of Bax protein was highest in the HE group. The expression of caspase-3 (cleaved form) protein was observed in the HE group. For the different types of muscle fiber, Bcl-2 protein expression in the soleus muscle was decreased in all groups. Bax protein expression in the soleus muscle was increased in the HE group only. Bcl-2 protein expression in the EDL muscle was decreased in the HE group, and Bax protein expression in the EDL muscle was increased in the ME and HE groups. Consequently, the protein expression related to the aged rats shows a difference according to the intensity of exercise. In addition, caspase-3 protein expression appeared in the HE group; however, in all amounts of intensity, DNA fragmentation was not observed. Therefore, apoptosis on skeletal muscles of aged mice can be intervened with optimal exercise. On the other hand, high intensity exercise can potentially accelerate the apoptosis of muscle fiber in aged rats.
Keywords
Exercise intensity; apoptosis; Bcl-2; Bax; caspase-3;
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1 Powers, S. K., D. Criswell, J. Lawler, L. L. Ji, D. R. Martin, A. Herb, G. Dudley. 1994. Influence of exercise and fiber type on antioxidant enzyme activity in rat skeletal muscle. Am. J. Physiol. 266, R375-R380.
2 Rice, K. M. and E. R. Blough. 2006. Sarcopenia-selated apoptosis is regulated differently in fast- and slow-twitch muscles of the aging F344/N ${\times}$ BN rat model. Mechanism of Aging and Development 127, 670-679.   DOI
3 Rice, K. M. and E. R. Blough. 2006. Sarcopenia-related apoptosis is regulated differently in fast and slow twitch muscles of the aging F344/N ${\times}$ BN rat model. Mechanism of Ageing and Development 670-679.
4 Sandri, M., U. Carraro, M. Ponhorska-Okolv, C. Rizzi., P. Arslan, D. Monti, and C. Franceschi. 1995. Apoptosis, DNA damage and ubiquitin expression in normal and mdx muscle fibers after exercise. FEBS. Lett. 373, 291-295.   DOI
5 Sandri, M., M. Podhorska-Okolow, V. Geromel, C. Rizzi, P. I. Arslan, C. Franc-esche, and U. Carraro. 1997. Exercise indyces myonuclear ubiquitination and apoptosis in dystrophin-deficient muscle of mice. J. Neuropathol. Exp. Neurol. 56, 45-57.   DOI
6 Danial, N. N., and S. J. Korsmeyer. 2004. Cell death: critical control points. Cell 116, 205-219.   DOI
7 Dirks, A. J. and C. Leeuwenburgh. 2006. Tumor necrosis factor $\alpha$ signaling in skeletal muscle: effects of age and caloric restriction. J. Nutri. Biochem. 17, 501-508.   DOI
8 Dirks, A. J. and C. Leeuwenburgh. 2004. Aging and lifelong calorie restriction result in adaptation of skeletal muscle apoptosis repressor, apoptosis-inducing factor, X-linked inhibitor of apoptosis, caspase-3, and caspase-12. Free Radic. Biol. Med. 36, 27-39.   DOI
9 Dirks, A. and C. Leeuwenburgh. 2002. Apoptosis in skeletal muscle with afina. Am. J. Physiol. Regul. Integr. Comp. Physiol. 282, 519-527.
10 Dreyer, H. C. and E. Volpi. 2005. Role of protein and amino acids in the patho -physiology and treatment of sarcopenia. J. Am. Cell Nutr. 24, 140-145.   DOI
11 Smith, J. 1996. Muscle growth factors, ubiqutin and apoptosis in dystrophic muscle: apoptosis decline with age in the mdx mouse. Basic. Appl. Myol. 6, 279-284.
12 Siu, P. M., R. W. Bruner, J. K. Martyn, and S. E. Alway. 2004. Apoptotic adaptations from exercise training in skeletal and cardiac muscles. FASEB. J. 18, 1150-1152.
13 Siu, P. M., E. E. Pistilli, M. J. Ryan, and S. E. Alway. 2005. Aging sustains the hypertrophy-associated elevation of apoptotic suppressor X-linked inhibitor of apoptotic protein (XIAP) in skeletal muscle during unloading. H. Gerontol. A Biol. Sci. Med. Sci. 60, 976-983.   DOI   ScienceOn
14 Siu, P. M. and S. E. Alway. 2006. Aging alters the reduction of pro-apoptotic signaling to loading-induced hyper-trophy. Exp. Gerontol. 41, 175-188.   DOI
15 Dupont-Versteegden, E. E. 2005. Apoptosis in muscle strophy: relevance to sarcopenia. Exp. Gerontol. 40, 473-418.   DOI
16 Ecochard, L., F. Lhenry, B. Sempore, and R. Favier. 2000. Skeletal muscle HSP72 level during endurance training: influence of peripheral arterial insufficiency. Pflugers Archv: European Journal of Physiology 440, 918-924.   DOI
17 Eva, M., A. Lisa, M. Cristina, B. Bert, L. Emanele, K. Masaaki, M. Daniel, R. Carlo, S. Stefano, and M. Sandri. 2009. Autophagy is required to maintain muscle mass. Cell Metabolism 10, 507-515.   DOI
18 Hughes, V. A., W. R. Frontera, R. Roubenoff, W. F. Evans, and M. A. Singh. 2002. Longitudinal changes in body composition in older men and women: role of body weight and physical activity. Am. J. Clin. Nutr. 76, 473-481.
19 Kannan, K. and S. K. Jain. 2000. Oxidative stress and apoptosis. Pathophysiology 7, 153-163.   DOI
20 Song, W., H. B. Kwak, and J. M. Lawler. 2006. Exercise training attenuates age-induced changes in apoptotic signaling in rat skeletal muscle. Antioxid. Redox Signal. 8, 517-528.   DOI
21 Szulc, P., F. Duboeuf, F. Marchand, and P. D. Delmas. 2004. Hormonal and lifestyle determinants of appendicular skeletal muscle mass in men; the MINOS study. Am. J. Clin. Nutr. 80, 496-503.
22 Thompson, H. S., P. M. Clarkson, and S. F. Scordilis. 2002. The repeated bout effect and heat shock proteins: Intramuscular HSP27 and HSP70 expression following two bouts of eccentric exercise in human. Acta Physiolgica Scandinavita 174, 47-56.   DOI
23 Tidball, J. G., D. E. Albrecht, B. E. Locknsgard, and M. J. Spencer. 1995. Apoptosis precedes necrosis ofdystrophin-deficient muscle. J. Cell Sci. 108, 2197-2204.
24 Vandervoort, A. A. 2002. Aging of the human neuromuscular system. Muscle Nerve. 25, 17-25.   DOI
25 Wyille, A. H., J. F. Kerrm, and A. R. Curie. 1980. Cell death: the significance of apoptosis. Int. Rev. Cytol. 68, 251-305.   DOI
26 Podhorska-Okolow, M., M. Sandri, S. Zampieri, B. Brun, K. Rossini, and U. Carraro. 1998. Apoptosis of myofibres and satellite cells: exercise-induced damage in skeletal muscle of the mouse. Neuropathol. Appl. Neurobiol. 24, 518-531.   DOI
27 Kocturk, S., B. M. Kayatekin, H. Rewmi, O. Acikgoz, C. Kaynak, and E. Ozer. 2008. The apoptotic response to strenuous exercise of the gastronemius and solues muscle fibers in rats. Eur. J. Appl. Physiol, 515-524.
28 Leeuwenbergh, C., C. M. Gurley, B. A. Strotman, and E. E. Dupont-Versteegden. 2005. Age-related differences in apoptosis with disuse atrophy in Soleus muscle. Am. J. Physiol. integr. Comp. Physiol. 288, R1288-1296.   DOI
29 Phillips, T. and C. Leeuwenburgh. 2005. Muscle fiber specific apoptosis and TNF-alpha signaling in sarcopenia are attenuated by life-long calorie restriction. Br. J. Cancer 86, 1012-1016.   DOI
30 Armstrong, R., and R. Phelps. 1984. Muscle fiber type composition of ran hindlimp. Am. J. Anat. 171, 259-272.   DOI
31 Baumgartner, R. N., K. M. Koehler, D. Gallagher, L. Romero, S. B. Heymsfield, R. R. Ross, P. J. Garry, and R. D. Lindeman. 1998. Epidemiology of sarcopenioa among the elderly in New Mexico. Am. J. Epidemiol. 147, 755-763.   DOI   ScienceOn
32 Bedford, T. G., C. M. Tipton, N. C. Wilson, R. A. Oppliger, and C. V. Gisolfi. 1979. Maximum oxygen consumption of rats and its changes with various experimental procedures. J. Appl. Physiol. 47, 1278-1283.
33 Chung, L. and Y. Ng. 2006. Age-related alterations in expression of apoptosis regulatory proteins and hear shock proteins in rat skeletal muscle. Biochim. Biophys. Acta. 1762, 103-109.   DOI   ScienceOn
34 Concordet, J. P. and A. Ferry. 1993. Physiological programmed cell death in thymo cytes is induced by physical stress (exercise). Am. J. Physiol. 265, C626-C629.
35 Alway, S. E., H. Degens, G. Krishnamurthy, and A. Chaudhrai. 2003. Denervation stimulates apoptosis but not Id2 expression in hindimb muscles of aged rats. J. Gerontology Series A: Biol. Sci. Med. Sci. 58, 687-697.   DOI