• Title/Summary/Keyword: fern$NaH_2PO_4$

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Effect of explant parts and medium components on in vitro regeneration in Osmunda cinnamomea var. forkiensis (꿩고비(Osmunda cinnamomea var. forkiensis) 기내 포자체 재생에 영향을 미치는 배양부위와 배지구성물질)

  • Kwon, Hyuk Joon;Shin, So Lim;Lee, Cheol Hee;Kim, Soo-Young
    • Journal of Plant Biotechnology
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    • v.44 no.4
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    • pp.448-453
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    • 2017
  • This study was carried out to find culture materials (explant parts) and medium components (medium type, sucrose and $NaH_2PO_4$ concentration) for in vitro propagation of Osmunda cinnamomea var. forkiensis sporophyte. The results of study: chopped segments of leaf blades, stipes, rhizomes and roots were cultured on a 1/2MS medium supplemented with 0.1% activated charcoal. Among these explant types, only the rhizome segments produced young sporophyte, regenerating vigorously on a 1/8MS medium. Adjusting the sucrose concentration to 2% and supplement to $50mg{\cdot}L^{-1}\;NaH_2PO_4$ in the 1/8MS medium proved to be more efficient for plant regeneration. Consequently, the addition of 0.1% activated charcoal to a modified 1/8MS medium (2% sucrose, $50mg{\cdot}L^{-1}\;NaH_2PO_4$, pH 5.8 and 0.8% agar) yielded the highest sporophyte regeneration.

Factors Affected on Plant Regeneration of Phyllitis scolopendrium (L.) Newm. In vitro (기내에서 변산일엽의 식물체 재생에 영향을 미치는 요인들)

  • Jeong Jin-A;Lee Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.365-373
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    • 2006
  • This study was conducted to develop the efficient propagation method of fern Phyllitis scolopendrium using In vitro culture. The influence of the origin of the donor explant sources (rhizome, stipe, three parts of blade) and the homogenization of explants was investigated. Rhizome and stipe explants showed the organogenic capacity among the five explant sources and plant regeneration was promoted by homogenization of culture material. Optimum condition for vigorous and excellent growth of multiple shoots was the half-strength MS medium with 1% sucrose concentration. Generally, addition of $NaH_2PO_4$ to media enhanced shoot multiplication. The highest rate of shoot proliferation was observed on the media containing $5{\mu}M$ NAA. Also, combination of activated charcoal $(0.1{\sim}0.2%)$ and growth regulators to growth medium prevented the formation of multiple bud primordia, 'nodule'-like bud clusters and improved the normal morphogenesis of sporopytes in P. scolopendrium.

Medium Composition Affecting In Vitro Plant Regeneration and Acclimation of Pteris cretica 'Wilsonii' (Pteris cretica 'Wilsonii'의 기내 식물체 재생에 미치는 배지구성물질의 영향 및 기외순화)

  • Shin, So-Lim;Lee, Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.22 no.5
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    • pp.394-402
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    • 2009
  • Adventitious shoots were induced from pinnae, petiole and rhizome in Pteris cretica 'Wilsonii' in order to develop the efficient mass propagation method, using in vitro culture. Only homogenized rhizome segments could regenerate young sporophytes. Efficient regeneration of multiple shoots was obtained on the one-eighth strength MS medium containing 1% sucrose, and $50mg{\cdot}L^{-1}$ $NaH_2PO_4$. To achieve higher rate of regeneration from rhizome segments, rhizome segments were exposed to growth regulators for 2 months and then subcultured on hormone-free medium. The greatest shoot regeneration was obtained by $1{\mu}M$ kinetin with $5{\mu}M$ NAA. BA was effective in formation of GGB (kind of meristems), but they showed low shoot regeneration rate. Plants obtained from present experiments were transplanted to examine good environmental conditions for acclimation. Juvenile plants obtained by the one-eighth strength MS medium showed highest survival rate and vigorous growth at the seedling stage.

Medium Composition Affecting In Vitro Regeneration of Matteuccia struthiopteris (청나래고사리의 기내 포자체 재생에 미치는 배지 구성물질의 영향)

  • Shin, So Lim;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.17 no.2
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    • pp.93-100
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    • 2009
  • This study was carried out to investigate the efficient in vitro mass propagation methods for juvenile sporophytes of Matteuccia struthiopteris. Chopped segments of pinnae, petiole and rhizome were cultured on 1/2MS with 0.1% activated charcoal. Among these explant sources only rhizome segments produced young sporophytes, regenerating vigorously on 1/2 MS medium. Adjusting sucrose concentration to 2% and supplement to $50mgL^{-1}$ $NaH_2PO_4$ in 1/2MS medium proved to be more efficient for plant regeneration. Various combinations of growth regulators such as kinetin, BA, NAA, and IBA were added to the growing media, and the best sporophyte regeneration was obtained by $1{\mu}M$ kinetin. The BA addition resulted in vigorous proliferation of meristematic tissues, but without differentiation to sporophytes. Three types of culture methods, solid using agar, liquid stationary, and liquid shaking culture, were employed with or without activated charcoal. The addition of 0.1% activated charcoal to modified 1/2MS media (2% sucrose, $50mgL^{-1}$ $NaH_2PO_4$, $1{\mu}M$ kinetin, pH 5.8 and 0.8% agar) yielded highest sporophyte regeneration in liquid shaking culture.

Effect of Culture Method and Medium Composition on Shoot Regeneration from Sporophytes of Cyrtomium caryotideum var. coreanum Nakai. (참쇠고비(Cyrtomium caryotideum) 포자체로부터의 식물체 재생에 미치는 배양방법 및 배지구성물질의 영향)

  • Jeong Jin-A;Lee Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.19 no.2
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    • pp.265-272
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    • 2006
  • This study was conducted to investigate the efficient propagation method of Cyrtomium caryoptideum var. coreanum by sporophyte culture. The influence of origin of explant sources (rhizome, blade, or stipe) and homogenization of culture materials on shoot regeneration were investigated. As a result, only rhizome explant exhibited the organogenic capacity and the shoot regeneration was promoted by homogenization of culture material. Vigorous and excellent growth of multiple shoots was induced on the half-strength of inorganic salts containing MS medium. It was appeared that optimum nitrogen content of shoot regeneration was half-strength of nitrogen containing MS medium (30mM) and optimum sucrose concentration was 1%. Addition of $NaH_2PO_4$ to culture medium generally enhanced shoot multiplication and promoted growth of the regenerants. The organogenic capacity of homogenized rhizomes was especially promoted on medium supplemented with $5{\mu}M$ kinetin plus $5{\mu}M$ IBA. The incorporation of $0.1\sim0.2%$ activated charcoal on medium supplemented with growth regulators prevented the formation of multiple bud primordia - nodule-like bud clusters and improved the normal morphogenesis of sporophytes.

Optimal Culture Conditions for Masspropagation of Gametophytes and Sporophytes of Pyrrosia linearifolia by Tissue Culture (조직배양을 이용한 우단일엽의 대량번식을 위한 전엽체와 포자체의 적정 배양조건)

  • Shin, So Lim;Lee, Cheol Hee
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.3
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    • pp.179-185
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    • 2010
  • This study was carried out to investigate the optimal culture conditions for gametophytes growth and sporophytes regeneration of Pyrrosia linearifolia in order to provide for the masspropagation system foundation of Pyrrosia linearifolia using their life cycle. Among many different media, 2MS medium was most effective in prothallus proliferation. Prothallus growth was promoted as the total concentration of nitrogen sources increased, and the best result was observed on 120 mM nitrogen. The best concentration of sucrose was 3%. The addition of 5~20 mM IAA, NAA, BA and kinetin promoted the propagation of prothallus. But 2iP demonstrated the most inhibitory effect on prothallus proliferation. Gametophytes shaking-cultured with liquid medium showed similar growth with solid medium and normal formation of reproductive organs. Shoot regeneration was most effective on 1/8MS medium, but growth was promoted on 1/2MS medium. For promotion of shoot regeneration and growth, the suitable concentrations of sucrose and $NaH_2PO_4$ were 1% and $50{\sim}100mg{\cdot}mL^{-1}$ in 1/8MS medium, respectively.