FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
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Optimal Culture Conditions for Masspropagation of Gametophytes and Sporophytes of Pyrrosia linearifolia by Tissue Culture

조직배양을 이용한 우단일엽의 대량번식을 위한 전엽체와 포자체의 적정 배양조건

  • Shin, So Lim (Department of Horticulture, Chungbuk National University) ;
  • Lee, Cheol Hee (Department of Horticulture, Chungbuk National University)
  • 신소림 (충북대학교 응용생명환경학부 원예학과) ;
  • 이철희 (충북대학교 응용생명환경학부 원예학과)
  • Received : 2010.08.05
  • Accepted : 2010.08.23
  • Published : 2010.09.30
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Abstract

This study was carried out to investigate the optimal culture conditions for gametophytes growth and sporophytes regeneration of Pyrrosia linearifolia in order to provide for the masspropagation system foundation of Pyrrosia linearifolia using their life cycle. Among many different media, 2MS medium was most effective in prothallus proliferation. Prothallus growth was promoted as the total concentration of nitrogen sources increased, and the best result was observed on 120 mM nitrogen. The best concentration of sucrose was 3%. The addition of 5~20 mM IAA, NAA, BA and kinetin promoted the propagation of prothallus. But 2iP demonstrated the most inhibitory effect on prothallus proliferation. Gametophytes shaking-cultured with liquid medium showed similar growth with solid medium and normal formation of reproductive organs. Shoot regeneration was most effective on 1/8MS medium, but growth was promoted on 1/2MS medium. For promotion of shoot regeneration and growth, the suitable concentrations of sucrose and $NaH_2PO_4$ were 1% and $50{\sim}100mg{\cdot}mL^{-1}$ in 1/8MS medium, respectively.

본 연구에서는 우단일엽(Pyrrosia linearifolia)의 전엽체 및 포자체의 생활환을 이용한 주년 대량번식체계의 기초를 제공하기 위하여 전엽체 증식 및 포자체를 이용한 식물체 재생에 미치는 기내 환경요소를 구명하였다. 전엽체 증식은 2MS배지에서 가장 왕성하였다. 질소급원 또한 첨가량이 많아질수록 증식율과 생육이 우수하였으며, 120mM 첨가구에서 생육이 가장 왕성하였다. 전엽체 증식에 가장 적합한 sucrose의 첨가농도는 3%였다. IAA, NAA, BA 및 kinetin을 $5{\sim}20{\mu}M$정도 첨가하는 것은 전엽체의 생육을 촉진하였으나, 2iP는 전엽체의 생육을 억제하였다. 액체배지에서는 전엽체의 생육이 억제되었으나, 진탕배양했을 때에는 고체배지와 증식량이 유사하였으며, 생식기관 또한 정상적으로 형성되었다. 포자체의 엽신과 근경의 절편을 다져서 배양한 결과, 근경 절편에서만 포자체가 재생되었다. 곱게 다진 근경의 절편은 1/8MS배지에서 포자체 재생이 가장 왕성하였으나, 재생된 포자체의 생육은 1/2MS배지에서 가장 우수하였다. 1/8MS배지에서 포자체 재생 및 생육을 촉진시킬 수 있는 적정 sucrose의 첨가 농도는 1%이며, $NaH_2PO_4$를 $50{\sim}100mg{\cdot}mL^{-1}$ 첨가하는 것은 균질배양한 근경의 절편에서 포자체가 형성되는 것을 촉진하였다.

Keywords

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