• Asian-Australasian Journal of Animal Sciences
• /
• 제26권3호
• /
• pp.386-393
• /
• 2013

This experiment was conducted to evaluate anti-Salmonella enteritidis (anti-SE) bacteriophage as feed additives to prevent Salmonella enteritidis in broilers. The experimental diets were formulated for 2 phases feeding trial, and 3 different levels (0.05, 0.1 and 0.2%) of anti-SE bacteriophage were supplemented in basal diet. The basal diet was regarded as the control treatment. A total of 320 1-d-old male broilers (Ross 308) were allotted by randomized complete block (RCB) design in 8 replicates with 10 chicks per pen. All birds were raised on rice hull bedding in ambient controlled environment and free access to feed and water. There were no significant differences in body weight gain, feed intake and feed conversion ratio (FCR) at terminal period among treatments (p>0.05). Relative weights of liver, spleen, abdominal fat and tissue muscle of breast obtained from each anti-SE bacteriophage treatment were similar to control, with a slightly higher value in anti-SE bacteriophage 0.2%. In addition, a numerical difference of glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT) and LDL cholesterol level was observed in the 0.2% anti-SE bacteriophage application even though blood profiles were not significantly affected by supplemented levels of anti-SE bacteriophage (p>0.05). In the result of a 14 d record after Salmonella enteritidis challenge of 160 birds from 4 previous treatments, mortality was linearly decreased with increasing anti-SE bacteriophage level (p<0.05), and Salmonella enteritidis concentration in the cecum was decreased with increasing levels of anti-SE bacteriophage (p<0.05). Based on the results of this study, it is considered that supplementation of 0.2% anti-SE bacteriophage may not cause any negative effect on growth, meat production, and it reduces mortality after Salmonella enteritidis challenge. These results imply to a possible use of anti-SE bacteriophage as an alternative feed additive instead of antibiotics in broilers diet.

• 한국수산과학회지
• /
• 제49권5호
• /
• pp.683-688
• /
• 2016

The effects of dietary supplementing natural additives [yacon (YC), ginger (GG), and blueberry (BB)] on growth, feed utilization, and body composition of juvenile rockfish were compared to those of ethoxyquin (EQ), which is commonly used to prevent rancidity of fish feed. A total of 480 fish were distributed randomly into 12 50-L flow-through tanks (40 fish/tank). Four experimental diets were prepared: the control diet (Con) with 0.01% EQ, and the YC, GG and BB diets containing 1% YC, GG, and BB instead of wheat flour. Each diet was assigned randomly to triplicate tanks of fish, which were hand fed to satiation twice daily for 8 weeks. Weight gain of fish fed the YC diet was higher than that of fish fed all other diets. Feed efficiency (FE), protein efficiency ratio (PER), and protein retention (PR) in fish fed the YC diet were higher than those in fish fed the other diets. Whole-body chemical composition of the fish was affected by the additive sources. In conclusion, dietary supplementation with GG effectively improved weight gain and feed utilization (FE, PER, and PR) of juvenile rockfish.

• Asian-Australasian Journal of Animal Sciences
• /
• 제2권3호
• /
• pp.346-348
• /
• 1989

• Asian-Australasian Journal of Animal Sciences
• /
• 제17권5호
• /
• pp.678-683
• /
• 2004

An experiment was carried out to study the effects of exogenous enzymes (cellulases and proteases), yeast culture and effective micro-organism (EM) culture on feed digestibility and the performance of rabbits fed rice bran rich diets over a period of ten weeks. Twenty four, 8 to 9 weeks old male and female New Zealand White rabbits were allotted to 4 dietary treatments; a basal (control) feed containing 43% rice bran, basal feed supplemented with either enzymes, yeast culture or EM. Individual feed intake, body weight gain, nutrient digestibility, carcass characteristics and feed cost were studied. Sex of the rabbits had no significant (p<0.05) influence on the parameters studied. The control group showed the lowest daily feed intake (104.8 g), body weight gain (12.8 g) and the highest feed/gain ratio (8.20 g/g). The highest daily feed intake (114.3 g), body weight gain (20.42 g) and the lowest feed/gain ratio (5.60) were observed with enzymes. Compared to the control, yeast significantly (p<0.05) improved the feed intake, body weight gain and feed/gain ratio by 4.9, 34.4 and 22.0%, respectively, while EM improved (p<0.05) them by 4.0, 32.6 and 21.6%, respectively. All the additives improved (p<0.05) the digestibility of dry matter, crude protein, crude fiber and energy by 4.9-8.7, 3.6-10.7, 5.9-8.3 and 4.3-6.4%, respectively. Higher weights of pancreas (by 38.5-56.4%) and caecum (by 13.1-26.8%, compared to the control) were recorded with all additives but liver weight was increased only by yeast (24.5%) and enzymes (26.7%). Significantly (p<0.05) higher carcass recovery percentages were observed with enzymes (60.55), yeast (60.47) and EM (56.60) as compared to the control (48.52). Enzymes, yeast and EM reduced (p<0.05) the feed cost per kg live weight by 23.8, 15.9 and 15.5%, respectively. Results revealed that enzymes, yeast culture and EM can be used to improve the feeding value of agro-industrial by-products for rabbits in Sri Lanka and thereby to reduce the feed cost. Under the present feeding system, enzyme supplement was the best.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권10호
• /
• pp.1495-1500
• /
• 2003

Two bio-assays were conducted to evaluate turmeric root powder and mannan-oligosaccharides (MOS) as alternatives to feed antibiotics for broilers. In one trial, one hundred and eighty 19-days old broilers assigned to 18 groups of 10 were fed on one of six experimental diets with three replicates during four weeks. The diets included a basal feed without additives and with either virginiamycin, MOS, or turmeric at 1, 2 and 3 g/kg, respectively. In the second trial, one hundred and forty four 21-days old broilers arranged in 16 groups of nine were fed on the first four diets with four replicates for a similar period. Virginiamycin, MOS and turmeric (1 g/kg) in the first trial generally improved the weight gain of broilers by 3.4, 6.2 and 5.3%, respectively. In the second trial they increased the weight gain significantly (p<0.05) by 8.8, 8.0 and 15.1%, respectively. Additives improved the feed efficiency up to 15.1% and carcass recovery up to 3.1% (p<0.05). Virginiamycin, MOS and turmeric (1 g/kg) markedly reduced the abdominal fat content from 1.91% BW in the control to 1.44, 0.97 and 1.2% BW, respectively, in the first trial. The corresponding values obtained in the second trial were 1.01, 0.55 and 0.6%, respectively as compared to 1.22% in the control group. All additives showed a remarkable inhibition of duodenal coliform bacteria, yeast and mould in the caecum, and all viable microbes in the ileum. A significant (p<0.05) improvement in energy and protein utilization could be recorded with supplemented diets except for high turmeric diets. Dietary 2 and 3 g/kg addition of turmeric reduced energy and protein utilization as well as fat deposition. Present results reveal that turmeric and MOS are satisfactory alternatives to antibiotics in broiler feeds. Both MOS and turmeric possess an antimicrobial effect in vivo. Turmeric may also depress fat deposition in broilers.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권9호
• /
• pp.1248-1254
• /
• 2012

The effect of silage additives on ensiling characteristics and nutritive value of Napier grass (Pennisetum purpureum) silages was studied. Napier grass silages were made with no additive, fermented juice of epiphytic lactic acid bacteria (FJLB), molasses or cassava meal. The ensiling characteristics were determined by ensiling Napier grass silages in airtight plastic pouches for 2, 4, 7, 14, 21 and 45 d. The effect of Napier grass silages treated with these additives on voluntary feed intake, digestibility, rumen fermentation and microbial rumen fermentation was determined in 4 fistulated cows using $4{\times}4$ Latin square design. The pH value of the treated silages rapidly decreased, and reached to the lowest value within 7 d of the start of fermentation, as compared to the control. Lactic acid content of silages treated with FJLB was stable at 14 d of fermentation and constant until 45 d of ensiling. At 45 d of ensiling, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of silage treated with cassava meal were significantly lower (p<0.05) than the others. In the feeding trial, the intake of silage increased (p<0.05) in the cow fed with the treated silage. Among the treatments, dry matter intake was the lowest in the silage treated with cassava meal. The organic matter, crude protein and NDF digestibility of the silage treated with molasses was higher than the silage without additive and the silage treated with FJLB. The rumen parameters: ruminal pH, ammonia-nitrogen ($NH_3$-N), volatile fatty acid (VFA), blood urea nitrogen (BUN) and bacterial populations were not significantly different among the treatments. In conclusion, these studies confirmed that the applying of molasses improved fermentative quality, feed intake and digestibility of Napier grass.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권3호
• /
• pp.373-381
• /
• 2020

There is a need for newer feed additives due to legal prohibition on inclusion of growth promoting antibiotics in livestock diets in several countries due to antimicrobial resistance. In this context, rare earth elements (REE) have gained attention among animal nutritionists as potential growth promoters. Currently, several studies have reported better weight gain, milk production, egg laying capacity and feed conversion efficiency among different breeds of farm animals following supplementation with REE, with however largely inconsistent results. Furthermore, REE supplementation has also shown to improve ruminal fibrolytic and proteolytic activities as well as flavor of meat with negligible residues in edible tissue, however the mechanism behind this action is still unclear. According to existing research, due to their poor absorption and similarity with calcium REE might exert their action locally on gut microbial populations within the gastrointestinal tract (GIT). Moreover, REE have also shown anti-inflammatory, anti-oxidative as well as immune stimulating effects. The present review aims to broaden the knowledge about use of REE as feed additives for livestock and sum up efficacy of REE supplementation on performance and health of animals by comparing the findings. Till date, researches with REE have shown properties that make them a promising, new and safe alternative feed additive but further exploration is recommended to optimize effects and clarify discrepancy of various results before practical proposals can be drafted.

• 한국가금학회지
• /
• 제31권3호
• /
• pp.193-198
• /
• 2004

첨가제 선택을 위한 목적으로 성장단계별 육계 사료에 햄 철 성분, 규산염, 설탕 제조 부산물, 목초액을 첨가하여 실시한 첨가제 비교시험에서, 사육성적은 동일한 계사 내에서도 환경조건이 좋은 위치에서 사육된 대조구가, 다른 시험구보다 좋은 성적을 보여 사육환경의 중요성을 확인하였으며, 시험구중에서는 햄철 성분 첨가구가 증체량이 많았고 규산염 첨가구의 증체성적이 가장 낮았다. 따라서 설탕 제조 부산물의 메틸기 절약 효과는 사육시간의 경과와 함께 낮아져 다른 첨가제의 효능보다 낮았다. 사료효율은 첨가제를 혼합하지 않은 대조구의 공간 배치 잇점으로 사료 섭취량이 적고 체중증가가 커서 사료 요구율이 낮았고, 시험구 중에서는 목초액과 햄철 성분 첨가구가 거의 동일한 사료효율을 보였다. 축산연구소에 의뢰한 육질 평가에서는 사육성적이 좋았던 대조구를 제외한 시험구 중에서는, 규산염 첨가구의 보수성이 가장 좋았으나 가열 감량이 많아 도계 후 제품의 실중량이 작아지는 단점으로 인하여, 상품화에는 부적합한 것으로 사려된다. 대조구를 제외한 시험구 중에서는 햄철 성분함유구가 전단력과 가열감량, 조지방 함유량에서 좋은 점수를 받았고, 회사에서 실시한 관능검사 결과와 사육성적을 고려하였을 때, 햄철 성분이 함유된 첨가제를 이용한 제품개발이 가장 좋을 것으로 생각된다.

• 한국양식학회지
• /
• 제17권2호
• /
• pp.109-114
• /
• 2004

유용 미생물과 한약재가 포함된 새로운 사료첨가제의 다양한 농도(0.3, 0.6 및 0.9%)에 따른 넙치의 간기능의 활성에 미치는 영향 및 사료첨가제의 특성에 대해서도 조사하였다. 첨가제에 포함된 유용미생물(유산균, 바실러스균, 효모 및 광합성균)의 총수는 한방천이 5.6${\times}$$10^{8}$ CFU/g이었으며, 어력천은 3.0${\times}$$10^{8}$ CFU/g 이었다. 앙식에 일반적으로 사용되는 습사료의 병원미생물(Edwardsiella tarda, Vibrio anguillarum and Streptorwccus sp.)은 모든 첨가군에 있어서 첨가제의 농도의존적으로 유의한 감소를 나타내었다. 간중량 지수는 0.3%의 첨가군에서 유의하게 증가하였다. 혈중의 단백질량은 모든 첨가군이 유의한 증가를 나타내었으나. 간장내의 단백질량은 대조군이 높은 수치를 나타내었다. 생체방어기작을 하는 catalase 및 superoxide dismutase는 0.3% 및 0.6% 첨가군에서 각각 높은 활성을 나타내었다. 이상의 결과로 한약재와 유용미생물 혼합 사료첨가제의 사용으로 넙치의 항산화효소의 활성을 증대시켜 어류스트레스의 면역증대를 기대할 수 있을 것으로 여겨진다.

• Journal of Animal Science and Technology
• /
• 제53권2호
• /
• pp.139-146
• /
• 2011

항산화물질인 astaxanthin을 함유한 균주 Xanthophyllomyces dendrohous를 급여하여 오리의 성장 및 육질에 미치는 영향을 확인하였다. 1일령 오리 450수를 공시하여 배합사료만을 급여한 대조구와 Xanthophyllomyces dendrohous가 함유된 사료첨가제를 사료의 0.1%, 0.2%씩 첨가한 처리구를 두었다. 오리의 성장과 육질을 분석한 결과, 평균증체량은 대조구에 비해 0.2% 처리구에서 유의적으로 높았고 사료요구율은 처리구가 유의적으로 높은 결과를 보였다. 일반성분검사 결과에서는 처리구의 오리고기 지방함량이 유의성 있게 낮았으며, 콜레스테롤 함량 역시 낮게 나타났다. 육질검사 결과 보수력, 육즙손실 및 전단력에 있어서도 처리구가 유의적으로 우수한 결과를 나타냈다. 지방산 함량분석 결과는 포화지방산인 palmitic acid과 stearic acid이 대조구에 비해 유의적으로 낮았으며 불포화지방산인 oleic acid과 linoleic acid는 대조구에 비해 유의적으로 높은 수준을 보였다. 본 연구결과 Xanthophyllomyces dendrohous가 함유된 사료첨가제의 처리는 오리의 성장 및 육질개선과 지방산 조성 등에 있어 효과가 인정되어 고품질의 오리육 생산에 기여할 것으로 생각된다.