• 한국자원식물학회지
• /
• 제11권2호
• /
• pp.202-209
• /
• 1998

Through total fatty acid analysis by the Gas chromatography, 17 different fatty acids were identified from the sap extracted from bark of Korean Rhus(Anacardiaceae), six species. Linoleic acid(C18 :2) and oleic acid(C18 : 1) were identified as major fatty acids for the species investigated, but except in R. chinensis.In the fatty acid composition for the species, one or two specific-fatty acid was detected, and its could be utilized as a useful taxonomic character.The result of cluster analysis by the 22 reliable characters in the fatty acid composition suggested first group was R.trichocarpa and R. succedanea, the second group, R.verniciflua, R.ambgua and R. sylvestris, but R.chinensis was a greatly independent species. The possible chemotaxonomic application of the fatty acid composition of bark in the genus Rhus was discussed.

• Journal of Animal Science and Technology
• /
• 제65권5호
• /
• pp.1081-1093
• /
• 2023

This study aimed to compare meat quality traits between Berkshire and crossbreed (Landrace × Yorkshire × Duroc), and to investigate the relationship between meat quality traits and fatty acid composition. 20 Berkshire and 20 crossbreed pigs were used to compare pork loin quality and to determine the relationship between measured variables. 23 variables were measured including proximate composition, pH, drip loss and cooking loss, Warner-Bratzler shear force, and fatty acid composition. Berkshire had higher moisture content, pH, water-holding capacity, saturated fatty acids, and redness than the crossbreed pig (p < 0.05). The fat content and polyunsaturated fatty acid were low (p < 0.05) in Berkshire. Correlation analysis showed a negatively correlation between moisture and fat content, and a positively correlation between saturated fatty acid and fat content. Moreover, saturated fatty acid and polyunsaturated fatty acid were negatively correlated. As a result of factor analysis and partial least square regression, saturated fatty acid and polyunsaturated fatty acid were estimated to be the main factors affecting quality characteristics of pork. Pig breed is associated with differences in meat quality, and fatty acid composition can have an effect on meat quality parameters.

• 한국식품위생안전성학회지
• /
• 제13권2호
• /
• pp.149-154
• /
• 1998

Vibrio cholerae non-O1 49균주와 V. Vibrio cholerae non-O1, V. mimicus, V. vulnificus와 V. parahaemolyticus의 균체 지방산(fatty acid methyl ester; FAME)을 gas liquid chromatogrphy로 분석하였다. 이들 분석자료를 통계학적으로 처리하여 Vibrio 종과 V. cholerae의 혈청형별 유연성을 비교 검토하였다. 검출된 지방산은 모두 41종이었고 분포량이 많은 것은 16:0, 16:1 cis 9, 18:1 trans 9/16/cis 11과 15:0 iso 2 OH/16:1 cis 9였다. 검출된 지방산 중에서 35종은 V. cholerae를 동정하는데 주요한 인자로 작용되었다 지방산분포를 UPGMA(비가중수리분석)으로 dendrogram을 작성한 결과 V. cholerae non-O!은 V. cholerae O1보다 V. choleare non-O1 중에서 O2, O5, O8, O10, O14, O27, O37, O39, O45와 O69의 총 10종류 혈청을 대상으로 지방산 조성에 의한 유사성을 검토한 결과 유사도가 92% 이상 수준에서 7개의 아종을 형성하여 혈청형과 지방산 조성간에는 유의할 만한 상관관계가 있었다. 따라서 V. cholerae non-O1의 동정 및 역학적인 조사시 지방산 분석은 유용하게 활용될수 있음을 알 수 있다.

• 보존과학회지
• /
• 제16권
• /
• pp.21-26
• /
• 2004

회곽의 구조를 가진 충청북도 보은의 유인서 부처 묘와 옥천의 무연고 분묘에서 출토된 수침 복식에 부착되어 있는 회백색 오염물질에 대한 적외선 분광 분석을 통해, 미라의 회백색 물질과 동일 성분인 지방산의 칼슘염이 주성분이며 지방산, 지방, 단백질이 소량 함유된, 사체의 지방이 분해되어 생성된 시랍으로 판명되었다. 또 시랍의 주성분이 지방산의 칼습염인 것은 사체의 뼈나 회곽의 성분인 석회와의 관련성이 있음을 알 수 있다. 그리고 지방산 분석을 통해 사체의 시랍에서 볼 수 있는 10-Hydroxyhexadecanoic acid 및 10-Hydroxyoctadecanoic acid가 확인되었고 지방산의 함량 분포패턴도 Palmitic acid와 같은 포화지방산의 함량은 높고, Oleic acid와 같은 불포화지방산의 함량이 낮은 시랍과 유사한 패턴을 보이고 있다. 이와 같은 결과로 미루어 보아 옥천과 보은에서 출토된 복식 표면의 회백색 물질은 미라로부터 이동된 시랍이라는 사실을 확인할 수 있었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권6호
• /
• pp.757-765
• /
• 2011

Fatty acid composition of beef intramuscular tissue is an important trait because high proportions of mono-unsaturated fatty acid are related to favorable beef flavor. In this study, we investigated the effects of genetic factors, such as stearoyl-CoA desaturase (SCD), on beef carcass traits, including fatty acid composition, in the Hanwoo. Analysis of fatty acids in Hanwoo was performed using a breed raised in Gyeonbuk province (n = 395). Compared to the homozygote, the GA, CT, and CT genotypes of exon 5 in the SCD polymorphism showed a higher content of oleic acid (p<0.05) and higher contents of mono-unsaturated fatty acid (p<0.05) and marbling scores (p<0.05) in intramuscular fat. Results of haplotype analysis showed a significant presence of unsaturated fatty acids and marbling score in the $ht1^*ht2$ and $ht2^*ht2$ groups (p<0.05). Furthermore, haplotype effects more powerful than a single gene were also observed. These ht1 and ht2 types also showed a significant difference in unsaturated fatty acids and marbling score, affecting beef flavor in the Hanwoo groups. Therefore, it can be inferred that the ht1 and ht2 types might be valuable new markers for use in improvement of Hanwoo.

• Nutrition Research and Practice
• /
• 제15권4호
• /
• pp.468-478
• /
• 2021

BACKGROUND/OBJECTIVES: This study aimed to analyze the association between dietary omega-3 fatty acid intake and depression in postmenopausal women using data from the Korea National Health and Nutrition Examination Survey (KNHANES) VI. SUBJECTS/METHODS: The KNHANES is a cross-sectional nationwide health and nutrition survey. Dietary data, including omega-3 fatty acids, were assessed using the 24-h recall method. Depression was evaluated using a survey questionnaire. The association between dietary omega-3 fatty acids and depression was evaluated using multivariate logistic regression analysis. Depression, according to the dietary omega-3 fatty acid intake, was expressed as the odds ratio (OR) with a 95% confidence interval (CI). A total of 4,150 postmenopausal women were included in the analysis. RESULTS: In the fully-adjusted model, the group with the highest dietary omega-3 fatty acid intake significantly showed lower prevalence of depression than the group with the lowest intake (OR, 0.52; 95% CI, 0.33-0.83); a significant linear trend was detected (P for trend = 0.04). According to the dose-response analysis using cubic restricted spline regression, this association was linear and monotonic (P for non-linearity = 0.32). CONCLUSIONS: In this study, the dietary omega-3 fatty acid intake in postmenopausal women was inversely proportional to depression in a dose-response manner. Large cohort studies are needed to verify the causality between omega-3 fatty acids and depression in Korean postmenopausal women.

• 한국식품영양학회지
• /
• 제24권3호
• /
• pp.471-479
• /
• 2011

This study analyzes flavor ingredients according to types of whisky and maturation periods based on total 40 different types of whisky that are mainly distributed to Korea via imports. Whisky was classified into four categories based on origin, and also into different categories based on maturity period, ingredients such as fusel alcohol, fatty acid, and fatty acid esters and proportion of patterns were analyzed. As a result of an analysis for fusel alcohol, high qualified types of alcohol including 3-methylbutanol, 2-methylbutanol, iso-butanol, and 1-propanol were detected from all Scotch whiskys, America whiskys, and Canadian whiskys. In particular, the proportional sum of 3-methylbutanol and 2-methylbutanol, and the sum of 3-methylbutanol and 2-methylbutanol/iso-butanol were regarded as core factors to determine each type of whisky. Acetaldehyde, ethyl acetate, and acetic acid increased as maturation and storage period became longer. As a result of the fatty acid and fatty acid ethyl ester analysis, the major fatty acids were dodecanoic acid and decanoic acid, both with detection of octanoic acid and hexanoic acid. However, dodecanoic acid, decanoic acid, and octanoic acid were lower than the detectable limit in American and Canadian whiskys, showing a unique phenomenon that hexanoic acid was detected only in very small amounts. Malt Scotch whisky showed higher significance than blended Scotch whisky, making it possible to classify types of whisky. Fatty acid ethyl ester contents showed significance with fatty acid either. In addition, changes in the whiskys based on maturation period were confirmed via proportions of fatty acids and fatty acid ethyl esters. In general, the proportion of fatty acids and fatty acid ethyl esters decreased as the storage period increased. This study provided basic data to classify types of whisky based on maturation periods by analyzing the proportion of flavor ingredients such as fusel alcohols, fatty acids, and fatty acid ethyl esters.

• Ocean Science Journal
• /
• 제40권3호
• /
• pp.145-153
• /
• 2005

Variation in the microbial biomass and community structure found in sediment of heavily polluted bays and the adjacent unpolluted areas were examined using phospholipid fatty acid analysis. Total microbial biomass and microbial community structure were responding to environmental determinants, sediment grain size, depth of sediment, and pollution due to petroleum hydrocarbons. The marker fatty acids of microeukaryotes and prokaryotes - aerobic, anaerobic, and sulfate-reducing bacteria - were detected in sediments of the areas studied. Analysis of the fatty acid profiles revealed wide variations in the community structure in sediments, depending on the extent of pollution, sediment depth, and sediment grain size. The abundance of specific bacterial fatty acids points to the dominance of prokaryotic organisms, whose composition differed among the stations. Fatty acid distributions in sediments suggest the high contribution of aerobic bacteria. Sediments of polluted sites were significantly enriched with anaerobic bacteria in comparison with clean areas. The contribution of this bacterial group increased with the depth of sediments. Anaerobic bacteria were predominantly present in muddy sediments, as evidenced from the fatty acid profiles. Relatively high concentrations of marker fatty acids of sulfate-reducing bacteria were associated with organic pollution in this site. Specific fatty acids of microeukaryotes were more abundant in surface sediments than in deeper sediment layers. Among the microeukaryotes, diatoms were an important component. Significant amounts of bacterial biomass, the predominance of bacterial biomarker fatty acids with abundance of anaerobic and sulfate-reducing bacteria are indicative of a prokaryotic consortium responsive to organic pollution.

• 한국식품조리과학회지
• /
• 제7권1호
• /
• pp.45-51
• /
• 1991

1. 유지의 지방산 조성 분석시 GC의 경우 Oleic acid/Elaidic acid를 분리 측정하지 못하였으나 그 외 지방산들(Myristic acid, Palmitic acid, Stearic acid, Linoleic acid, Lindenic acid, Arachidonic acid)에 있어서는 재현성이 높았으며 HPLC는 Stearic acid, Elaidic acid, Linoleic acid Arachidonic acid 검출에 높은 재현성을 나타냈으나 Palmitic acid/Oleic acid를 분리하지 못하였으며 CGC는 Elaidic acid를 제외한 모든 지방산들에 있어서 좋은 측정능력을 보였주었다. 2. 지방산의 cis/trans 기하 이성체의 측정시에는 CGC가 가장 능력이 뛰어났고 HPLC도 양호하였으나 GC는 Oleic acid/Elaidic acid 이성체 측정이 불가능하였다. 또한 각 지방산 분석에 소요된 평균 시간은 GC가 7.21분, CGC가 9.84분, 그리고 HPLC는 24.48분으로서 GC와 CGC의 경우는 비슷하나 HPLC는 다른 기기에 비해 약2.5∼3배의 많은 시간이 소요되었다. 3. 이들 3가지 분석기기로 실제 유지식품인 대두유(CSOY, DSOY)의 지방산조성을 분석한 결과, 정제된 대두유(CSOY)는 Linoleic acid, Oleic acid, Palmitic acid, Linolenic acid가 주요구성 지방산인 반면 Stearic acid는 소량으로 함유되어 있었으며, 미정제 대두유(DSOY)에서는 Linoleic acid, Oleic acid, Palmitic acid, Stearic acid가 주요 구성 지방산이었고 Linolenic acid는 아주 미량 함유되어 있었다. 이때 3가지 분석기기의 대두유의 지방산 분석능력은 표준지방산 분석의 지방산 분석능력은 표준지방산 분석시에 나타난 결과와 동일하였다. 따라서 GC, CGC및 HPLC의 분석특징으로 GC의 경우 분석소요시간이 CGC나 HPLC에 비해 짧은 반면 이성체 분리가 불가능하였으며 HPLC는 분석소요시간이 다른 기기에 비해 다소 긴 반면 이성체 측정이 가능하였고 포화지방산보다는 불포화지방산 측정에 다소 유리하였다. 그리고 CGC의 분석특성에 있어서 재현성, 분석 소요시간 및 이성체 분리능력이 GC나 HPCL에 비해 우수한 것으로 평가되었다.

• Journal of Microbiology and Biotechnology
• /
• 제19권10호
• /
• pp.1098-1102
• /
• 2009

Gamma-linolenic acid (GLA, C18:3 ${\Delta}^{6,9,12}$) is synthesized by a delta-6 fatty acid desaturase using linoleic acid (LA, C18:2 ${\Delta}^{9,12}$) as a substrate. To enable the production of GLA in the conventional yeast Pichia pastoris, we have isolated a cDNA encoding the delta-6 fatty acid desaturase from Cunninghamella echinulata MIAN6 and confirmed its function by heterogeneous expression in P. pastoris. Sequence analysis indicated that this cDNA sequence has an open reading frame of 1,404 bp, which encodes a 52 kDa peptide of 468 amino acids. This sequence has 64% identity to the previously reported delta-6 fatty acid desaturase from Rhizopus oryzae. The polypeptide has a cytochrome b5 domain at the N-terminus including the HPGG motif in the heme-binding region, as reported for other delta-6 fatty acid desaturases. In addition, this enzyme differs from other desaturases by the presence of three possible N-linked glycosylation sites. Analysis of the fatty acid composition demonstrated the accumulation of GLA to the level of 3.1% of the total fatty acids. Notably, the amounts of ginkgolic acid (C17:1) and palmitic acid (C16:0) were increased from 1.3% to 29.6% and from 15% to 33%, respectively. These results reveal that the modification of the fatty acid biosynthetic pathway by genetic manipulation in order to produce specific polyunsaturated fatty acids in P. pastoris is a promising technique.