• Ocean and Polar Research
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• v.44 no.4
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• pp.319-329
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• 2022

The paramyxean parasite Marteilioides chungmuensis infects the cytoplasm of the eggs of Pacific oysters Crassostrea gigas , resulting in spawning failure of the infected females. Such infected eggs appear as bump-like nodules on the body in late fall when most of the uninfected females complete spawning. In this study, we estimated the quantity of the infected eggs using an enzyme-linked immunosorbent assay (ELISA), which is destroyed by M. chungmuensis parasitism. In December, the infected oysters collected from Tongyoung on the south coast exhibited numerous yellowish bump-like nodules as signs of infection. In histology, the infected oysters exhibited mature eggs in the follicle, which were heavily infiltrated by hemocytes. ELISA indicated that the infected egg mass accounted for 7.52±5.50 percent of the body weight, suggesting the ovarian parasite causes substantial reproductive loss. Histology also indicated that the infected oysters are in a poor nutritional condition, as the digestive gland atrophy (DGA) level is comparatively higher than the uninfected oyster. The total carbohydrate contents in the infected oysters (108.68±44.41 mg/g dry wt) were significantly lower than in uninfected oysters (269.76±50.97 mg/g dry wt), suggesting that M. chungmuensis parasitism also affected the energy storage capacity of the host during the resting stage.

• Journal of Veterinary Science
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• v.21 no.3
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• pp.50.1-50.16
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• 2020

Background: Porcine parvovirus (PPV) is a single-stranded DNA virus that causes porcine reproductive failure. It is of critical importance to study PPV pathogenesis for the prevention and control of the disease. NS1, a PPV non-structural protein, is participated in viral DNA replication, transcriptional regulation, and cytotoxicity. Our previous research showed that PPV can activate nuclear factor kappa B (NF-κB) signaling pathway and then up-regulate the expression of interleukin (IL)-6. Objectives: Herein, the purpose of this study is to determine whether the non-structural protein NS1 of PPV also has the same function. Methods: Real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay, western blot, immunofluorescence assay and small interfering RNA (siRNA) were used. Results: Our findings demonstrated that PPV NS1 protein can up-regulate the expression levels of IL-6 and tumor necrosis factor-alpha in a dose-dependent manner. Moreover, PPV NS1 protein was found to induce the phosphorylation of IκBα, then leading to the phosphorylation and nuclear translocation of NF-κB. In addition, the NS1 protein activated the upstream pathways of NF-κB. Meanwhile, TLR2-siRNA assay showed TLR2 plays an important role in the activation of NF-κB signaling pathway induced by PPV-NS1. Conclusions: These findings indicated that PPV NS1 protein induced the up-regulated of IL-6 expression through activating the TLR2 and NF-κB signaling pathways. In conclusion, these findings provide a new avenue to study the innate immune mechanism of PPV infection.

• Journal of Aerospace System Engineering
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• v.17 no.4
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• pp.104-109
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• 2023

The Cube Satellite Contest has been held six times as of August 2023, and a total of 21 teams have been selected. Fifteen Cube Satellites selected in previous contests were successfully launched and entered into low-Earth orbit. The six Cube Satellites selected in the sixth contest in 2022 are currently undergoing detailed design, and are scheduled to be launched in 2025 using a Korean launch vehicle. In this study, we analyzed the initial operation reports submitted by the selected teams of the Cube Satellite Contest in 2012, 2013, 2015, 2017, and 2019 to assess mission performance and identify causes of mission failure. Based on the submitted reports, an improvement scheme to enhance mission success for future Cube Satellites is proposed.

• Journal of the Korean Geotechnical Society
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• v.24 no.11
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• pp.17-24
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• 2008

It is known that scour at bridge piers is one of the leading causes of bridge failure. However, the mechanism of flow around a pier structure is so complicated that it is difficult to establish a general empirical model to provide accurate estimation for scour. Especially, each of the proposed empirical formula yields good results for a particular data set but can't show reliable predictability for various scouring data set. In this study, an alternative approach, that is, artificial neural networks (ANN), is proposed to estimate the local scour depth with numerous field data base. The local scour depth was modeled as a function of seven variables; pier shape, pier width, pier length, skew angle, stream velocity, water depth, $D_{50}$. 426 field data were used for the training and testing of ANN model. The predicted results showed that the neural network could provide a better alternative to the empirical equations.

• Journal of the Korean Geotechnical Society
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• v.25 no.2
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• pp.25-35
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• 2009

Slope failures are one of the significant disasters which causes lots of human casualties and huge financial losses every year. Previous researches on the slope failure have indicated that most accidents are closely related to the pore water pressure in the slope due to rainfall during the rainy seasons or stormy weather conditions. It would be therefore appropriate to consider the effect of pore water pressure in the design of slopes. As the existing slopes are generally reinforced by plants and other slope protecting measures, their boundary conditions are highly complicated. In this paper an attempt to develop a new modeling and analysis technique of slopes is proposed by including pore water pressure and adopting the coupled finite element method. Non-reinforced and reinforced slope models are considered. Representative analysis showed that the numerical modeling considering pore water pressure is appropriate in slope stability analysis. Flow behavior in the slopes is identified for various hydraulic boundary conditions. It is also shown that the effect of pore water pressure on slope stability is significant.

• Journal of the Korean Geotechnical Society
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• v.26 no.4
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• pp.59-68
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• 2010

Piping, a common form of internal embankment erosion, is caused by progressive movement of soil particles through an embankment. The phenomenon commonly occurs with precursory signs of development of fractures in dam structures, but also occurs without any noticeable signs in dams that showed satisfactory dam performance for several years, due to dissolution of soluble material in an embankment. While piping accounts for nearly 50% of the causes for dam failure, few studies have been made for systematic evaluation of the phenomenon. In this study, we attempted to monitor the changes in electrical resistivities of fill-dam material while a saddle dam is dismantled for the construction of emergency spillways of Daechung dam. Two artificial subhorizontal boreholes were drilled into the embankment structure to simulate piping along the two artificial flow channels. Monitoring of changes in electrical resistivity showed an increase in resistivity values during piping. Thus, the investigation of resistivity over time could be an effective method for piping prediction.

• Korea Science and Art Forum
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• v.24
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• pp.1-13
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• 2016

The aim of this study is to find the reasons of success and failure of digital animations in terms of 'immersion.' For the purpose, the causes of immersion were analyzed through complex comparison about concepts and stories of two animation films with similar size of budget: 'Shrek 2' which ranked number 1 in the box-office of animation and 'Mars Needs Moms' which ranked number 172 in the same category. The complex results showed that immersion becomes bigger with creativity and popularity of the characters' outer concept, diversity and organic of the characters' inner concept, and diversity and consistency of the background. Moreover, gradual arrangement of characters' appearance in the story and increasing number of the characters, and visual changes around the plot heightening with a well-organized passage increase immersion. After all, immersion of digital animation requires developing creative, diverse, and popular concept and arranging and strengthening strategic well-organized plot of parts and the whole. This is the key to convergence it.

• The Korean Journal of Franchise Management
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• v.6 no.2
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• pp.31-49
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• 2015

The purpose of this study was to propose a policy development plan for win-win growth between franchisor and franchisee by investigating success factors of win-win growth on the basis of the problems between franchisor and franchisee. There are many small scale franchise businesses in Korea that have not achieved an economy of scale compared to the developed countries. The conflicts between franchisor and franchisee have continued due to a lack of social trust which comes from an excessively high ratio of business failure and big firms' expansion into franchise business, then again, the perception of relations between the topdog and the underdog causes many problems. As franchise is the industry based on common people that helps service industry advance and competitiveness of the self-employed improve, it needs to be activated as the higher value-added business by promoting establishment of business and strengthening foundation of related industry. A matter of mutual cooperation and win-win growth between franchisor and franchisee is important not only for the sound development of franchise industry but also for the stable growth of nation's economy. Thus, this study suggested a policy development plan for win-win growth between franchisor and franchisee by analyzing success factor of win-win growth based on the problems between franchisor and franchisee. The results of the study provide the important strategic way towards mutual growth and maintaining cooperative relationship between franchisor and franchisee in Korean franchise industry.

• Journal of the Korean Geotechnical Society
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• v.39 no.12
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• pp.103-114
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• 2023

Monitoring the site of an underground construction wall is crucial to confirm the stability of the supports and ground due to excavation. In particular, it is essential to maintain the accuracy of a load cell gauge, which identifies the load of the support transmitted from the excavated ground. However, research on verification methods and regulations that can identify the accuracy of load cell gauges at construction sites is inadequate, which is a problem as load cell gauges are installed without proper performance inspections. In this study, performance tests were conducted by a complete investigation of load cell gauges sold in Korea and comparing them with foreign products to determine defect causes. In addition, the criteria for selecting a load cell gauge were presented, and the results of this study were considered to help select a highly reliable load cell gauge.

• Korean Circulation Journal
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• v.53 no.6
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• pp.351-366
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• 2023

Along with the development of immunosuppressive drugs, major advances on xenotransplantation were achieved by understanding the immunobiology of xenograft rejection. Most importantly, three predominant carbohydrate antigens on porcine endothelial cells were key elements provoking hyperacute rejection: α1,3-galactose, SDa blood group antigen, and N-glycolylneuraminic acid. Preformed antibodies binding to the porcine major xenoantigen causes complement activation and endothelial cell activation, leading to xenograft injury and intravascular thrombosis. Recent advances in genetic engineering enabled knock-outs of these major xenoantigens, thus producing xenografts with less hyperacute rejection rates. Another milestone in the history of xenotransplantation was the development of co-stimulation blockaded strategy. Unlike allotransplantation, xenotransplantation requires blockade of CD40-CD40L pathway to prevent T-cell dependent B-cell activation and antibody production. In 2010s, advanced genetic engineering of xenograft by inducing the expression of multiple human transgenes became available. So-called 'multi-gene' xenografts expressing human transgenes such as thrombomodulin and endothelial protein C receptor were introduced, which resulted in the reduction of thrombotic events and improvement of xenograft survival. Still, there are many limitations to clinical translation of cardiac xenotransplantation. Along with technical challenges, zoonotic infection and physiological discordances are major obstacles. Social barriers including healthcare costs also need to be addressed. Although there are several remaining obstacles to overcome, xenotransplantation would surely become the novel option for millions of patients with end-stage heart failure who have limited options to traditional therapeutics.