Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Effects of Ovarian Parasite Marteilioides chungmuensis on the Reproduction of the Pacific Oyster Crassostrea gigas Assessed by Histology and Enzyme-Linked Immunosorbent Assay (ELISA)

  • Yanin, Limpanont (Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University) ;
  • Hyun-Sil, Kang (Tidal Flat Research Center, National Institute of Fisheries and Science) ;
  • Young-Ghan, Cho (Department of Marine Life Science and Marine Science Institue (BK21 FOUR), Jeju National University) ;
  • Jong-Seop, Shin (Department of Marine Life Science and Marine Science Institue (BK21 FOUR), Jeju National University) ;
  • Nobuhisa, Kajino (Department of Marine Life Science and Marine Science Institue (BK21 FOUR), Jeju National University) ;
  • Jeong-Hwa, Kim (Department of Marine Life Science and Marine Science Institue (BK21 FOUR), Jeju National University) ;
  • Hyun-Ki, Hong (Department of Marine Life Science and Marine Science Institue (BK21 FOUR), Jeju National University) ;
  • Kwang-Sik, Choi (Department of Marine Life Science and Marine Science Institue (BK21 FOUR), Jeju National University)
  • Received : 2022.09.14
  • Accepted : 2022.10.18
  • Published : 2022.12.30
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Abstract

The paramyxean parasite Marteilioides chungmuensis infects the cytoplasm of the eggs of Pacific oysters Crassostrea gigas , resulting in spawning failure of the infected females. Such infected eggs appear as bump-like nodules on the body in late fall when most of the uninfected females complete spawning. In this study, we estimated the quantity of the infected eggs using an enzyme-linked immunosorbent assay (ELISA), which is destroyed by M. chungmuensis parasitism. In December, the infected oysters collected from Tongyoung on the south coast exhibited numerous yellowish bump-like nodules as signs of infection. In histology, the infected oysters exhibited mature eggs in the follicle, which were heavily infiltrated by hemocytes. ELISA indicated that the infected egg mass accounted for 7.52±5.50 percent of the body weight, suggesting the ovarian parasite causes substantial reproductive loss. Histology also indicated that the infected oysters are in a poor nutritional condition, as the digestive gland atrophy (DGA) level is comparatively higher than the uninfected oyster. The total carbohydrate contents in the infected oysters (108.68±44.41 mg/g dry wt) were significantly lower than in uninfected oysters (269.76±50.97 mg/g dry wt), suggesting that M. chungmuensis parasitism also affected the energy storage capacity of the host during the resting stage.

Keywords

Acknowledgement

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2021R1C1C2004304), and global research grant of Jeju National University 2022.

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