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생쥐 배아의 전사와 발생에서 DNA/RNA 메틸화의 역할

  • 김종월
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 1998.07a
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    • pp.32-33
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    • 1998
  • 생물체에서 유전외적 변형의 하나인 DNA 메틸화는 cis-acting factor의 조성변화를 통하여 세포특이 유전자의 발현과 virus latency, genomic imprinting, mutagenesis등과 같은 생물학적 효과를 나타내는 것으로 알려져 있다.(reviewed by Olle Heby, 1995). 5-azaCR, 5-azaCdR 그리고 6-azaCR의 처리결과는 배아자체의 DNA 메틸화의 유지가 정상발생에 필수적임을 알 수 있으며, 메틸화에 의한 배아발생 조절기작이 존재함을 암시하고 있다. 이러한 과정에서 5-azaCR과 5-azaCdR은 서로다른 경로를 통하여 배아발생에 관여함을 보여주었다. 즉, 5-azaCdR은 주로 DNA에 incorporation되어 작용하는 것으로 여겨지며, 5-azaCR은 DNA 보다는 RNA에 incorporation되어 작용하는 것으로 나타났다. 그리고, 비록 소수의 유전자만이 조사되었지만, 5-azaCdR의 incorporation에 의한 cis-acting factor의 변화는 전사인자인 c-myc proto-oncogene과 fluid 수송에 관여하는 $Na^{+}$, $K^{+}$-ATPase 유전자의 전사를 억제하지 않았다. 반면, 5-azaCR의 RNA로의 incorporation은 전사인자인 c-myc proto-oncogene의 전사를 억제하였으며, 연이어 fluid 수송에 관련되어있는 $Na^{+}$, $K^{+}$-ATPase 유전자의 전사를 억제하였다. 이것은 아마도 RNA로 incorporation된 5-azaCR은 RNA의 post-transcriptional processing에 영향을 주어 trans-acting factor의 조성을 변화, 전사적 repression을 유발한 것으로 사료된다. 생쥐 착상전 초기배아에서 DNA 메틸화는 short-term하게는 cis-acting factor로써 전사적 수준에서 유전자발현 조절하며, 그리고 유전자발현을 통하여 long-term하게는 배아발생에 관여 할 것이라고 사료된다.

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A study of Fatigue Crack Growth Behavior and Crack Closure in 5083-O Aluminum Alloy (5083-0 알루미늄合金의 疲勞균열進展 擧動과 균열닫힘에 관한 硏究)

  • 박영조;김정규;김일현
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.10 no.2
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    • pp.208-214
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    • 1986
  • To establish the evaluation of the fatigue crack growth behavior in 5083-O aluminum alloy, constant load-amplitude fatigue crack growth tests were carried out under the small scale yielding conditions. Crack length and closure of this material were measured by the compliance method using a clip-on gage. The main results obtained as follows: The fatigue crack growth rate against stress intensity factor range .DELTA.K exhibits the trilinear form with two transitions at the growth rate 5.5*10$^{-6}$ and 5.5*10$^{-5}$ mm/cycle, in the so-caled Region II. The trilinear form appears still in the plot of growth rate versus effective stress intensity factor range .DELTA. $K_{eff}$. Stress ratio R affects the relationship of crack growth rates versus .DELTA.K but does not affect the reation of crack growth rate versus .DELTA. $K_{eff}$. The experimental results indicate that the effective stress intensity range ratio U depends on the maximum stress intensity factor $K_{max}$, but not on the stress ratio R.o R.R.

A Study on the Body Types of the Chinese Women (I) -Focusing on Beijing and Shanghai- (중국 성인 여성의 체형 연구(I) -북경과 상해에 거주하는 여성을 중심으로-)

  • Lim, Soon;Sohn, Hee-Soon;Seok, Hye-Jung
    • Journal of the Korean Society of Clothing and Textiles
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    • v.27 no.7
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    • pp.831-842
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    • 2003
  • The purpose of this study is to analyze body types of Chinese women and provide concrete information on it by classifying them into several representative groups. As for the method of this study, statistical analysis is made of 79 items. This is done from July 18 to Aug 07, 2002, 525 female subjects from age 20 to 49 participated in this study. They all live in Beijing and Shanghai in China. The results of this study are as follows. 1. Means, standard deviations, the maximum and minimum of 19 items are extracted. The height and girth item have a high standard deviations. 2. 8 factors are extracted by using factor analysis. Factor 1: body obesity, Factor 2: vertical body size, Factor 3: upper body length Factor 4: size of ankles, Factor 5: angle of shoulders, Factor 6: length of hip Factor 7: size of shoulder, Factor 8: shape of chest 3. The body types of Chinese women are classified into 5 sub groups from the result of the Cluster analysis.

A Study on the Structural Causal Relationship of Marketing Stimulating Factors on Online Impulse Purchase through Consumer Value: Focusing on the Mediating Effect of Consumer Value

  • KWON, Lee-Seung;LEE, Jae-Min
    • Journal of Wellbeing Management and Applied Psychology
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    • v.5 no.3
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    • pp.7-17
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    • 2022
  • Purpose: With the introduction of Web 2.0, the advent of smartphones, and the recent outbreak of COVID-19 worldwide, interest in online shopping has soared. In an online shopping environment, the desire for impulse buying increases compared to offline shopping. Research design, data and methodology: In this study, three marketing stimulating factors such as product factor, price factor, and facilitating factor were selected which affect impulse buying for clothing and accessory products in the Korean online shopping environment. The mechanism of causal relationship among them, and the role customer value between marketing stimulating factor and impulse buying was analyzed. Results: The analysis results are as follows. First, the product factor had a significant positive effect on consumer value and impulse buying. Second, the price factor also had a significant positive effect on consumer value and impulse buying. Conclusions: Although the facilitating factor had no significant influence on consumer value and impulse buying, the indirect effect through consumer value was positively significant. Consumer value had a positive mediating effect on impulse purchase in the order of price factor, product factor, and promotion factor.

Dielectric and Piezoelectric Properties of 0.57Pb(Sc1/2Nb1/2)O3-0.43PbTiO3 Ceramics with Dopant Additions (도펀트 첨가에 따른 0.57Pb(Sc1/2Nb1/2)O3-0.43PbTiO3 세라믹스의 유전 및 압전특성)

  • Ji, Seung-Han;Kwon, Sang-Jik
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.20 no.2
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    • pp.124-129
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    • 2007
  • Dielectric and piezoelectric properties of $0.57Pb(Sc_{1/2}Nb_{1/2})O_{3}-0.43PbTiO_{3}$, which is the morphotropic phase boundary composition for the PSN-PT system, were investigated as a function of $Fe_{2}O_{3},\;Nb_{2}O_{5}\;and\;MnO_{2}$ addition 0 wt% to 0.9 wt%. The maximum dielectric constant of ${\varepsilon}_{33}/{\varepsilon}_{o}=2054$ and the minimum dielectric loss of $tan{\delta}=0.37\;%$ at room temperature were obtained at 0.1 wt% of $Fe_{2}O_{3}$ and 0.5 wt% of $MnO_{2}$ addition, respectively. With addition of 0.5 wt% $Nb_{2}O_{5}$ and $0.5\;wt%\;MnO_{2}$, the electromechanical coupling factor $k_{p}$ and mecanical quality factor $Q_{m}$ were significantly increased, respectively. The maximum electromechanical coupling factor $k_{p}=61.5\;%$ was obtained by addition of $Nb_{2}O_{5}$ and high mechanical quality factor $Q_{m}=919$ was obtained by addition of $MnO_{2}$. The $Q_{m}(=919)$ value is 3.3 times larger than that of non-doped 0.57PSN-0.43PT ceramics.

Preparation of Microporous Silica Membrane from TEOS-$H_2O$ System and Separation Of $H_2$-$N_2$ Gas Mixture (TEOS-$H_2O$계로부터 다공성 실리카 막의 제조 및 수소-질소 혼합기체의 분리)

  • 강태범;이현경;이용택
    • Membrane Journal
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    • v.10 no.2
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    • pp.55-65
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    • 2000
  • The porous silica membrane was prepared from Si(${OC}_2H_5)_4-H_2O$ system by sol-gel method. To investigate the characteristics of gels and porous silica membrane, we examined gels and porous silica membrane using TG-DTA, X-ray diffractometer, IR spectrophotometer, BET, SEM and TEM. The optimum mole ratio of Si(OC$_2$H$_{5}$)$_4$ : $H_2O$ $C_2$H$_{5}$OH for porous silica membrane was 1 : 4.5 : 4. The porous silica membrane was obtained by heat treatment of the gel above 700 $^{\circ}C$. The specific surface area of sintered gel was 3.8 $m^2$/g to 902.3 $m^2$/g at 100 $^{\circ}C$ to 1100 $^{\circ}C$ The pore size of sintered gel was in the range 20 $\AA$~ 50$\AA$. The particle size of sintered gel was 15 nm to 30 nm at 30$0^{\circ}C$ to 700$^{\circ}C$. The performance of the porous silica membrane was investigated for the separation of $H_2$/$N_2$ gas mixture. Gas separation through porous silica membrane depends upon Knudsen flow and surface flow. The veal separation factor($\alpha$) of $H_2$/$N_2$ was 5.17 at 155.15 cmHg and $25^{\circ}C$. The real separation factor($\alpha$), head separation factor($\beta$), and tail separation factor( $\bar{B}$) increased as the pressure of permeation cell Increased.sed.

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LOCALLY SEMICOMPLETE DIGRAPHS WITH A FACTOR COMPOSED OF k CYCLES

  • Gould, Ronald J.;Guo, Yubao
    • Journal of the Korean Mathematical Society
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    • v.41 no.5
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    • pp.895-912
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    • 2004
  • A digraph is locally semicomplete if for every vertex $\chi$, the set of in-neighbors as well as the set of out-neighbors of $\chi$ induce semicomplete digraphs. Let D be a k-connected locally semicomplete digraph with k $\geq$ 3 and g denote the length of a longest induced cycle of D. It is shown that if D has at least 7(k-1)g vertices, then D has a factor composed of k cycles; furthermore, if D is semicomplete and with at least 5k + 1 vertices, then D has a factor composed of k cycles and one of the cycles is of length at most 5. Our results generalize those of [3] for tournaments to locally semicomplete digraphs.

An Analysis of Magnet Unit of Electro-Pneumtic Control Valve Positioner (전공식 콘트롤 밸브 Positioner 용 Magnet Unit의 해석)

  • 김성재;김지원;조순철;정선태;유형근;전찬구;고택범
    • Journal of the Korean Magnetics Society
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    • v.7 no.6
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    • pp.321-326
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    • 1997
  • We analyzed an important part of control valve, magnet unit, which is used to control the fluid. Magnetic circuit which is composed of magnet and yoke is analyzed using finite element method. Then, flux density and coil force were calculated and compared with those of measured. According to the simulation results, the gap field, force constant, and permeance coefficient were 3~5 kG, 27.5 N/A, 22.1, respectively, which corresponded reasonably well with the measured values. We also obtained reluctance factor of 1.1 and fringing factor of 1.4 by simulation.

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Crack growth behavior in the lntegrally stiffened plates(1) -Numerical evaluation of SIF (일체형 보강판의 균열성장거동(I)-SIF의 수치해석)

  • Rhee, Hwan-Woo
    • Journal of the Korean Society for Precision Engineering
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    • v.14 no.5
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    • pp.150-156
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    • 1997
  • Three dimensional finite element analysis was conducted to estimate the effect of shape parameters (plate width and thickness) on the stress intensity factor for crack in the integrally stiffened plate. Analysis was done for width ratios of 0.5, 0.75, 1.0, 1.5, 2.0, 2.5, and thickness ratios of 2, 3, 4, 6. Based on these results, an empirical equation of geometry factor is formulated as a function of crack length and thickness ratio.

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Electrical Properties and Temperature Stability of resonant Frequency with Zr/Ti ratio in PSN-PMN-PZT Ceramics (PSN-PMN-PZT 세라믹스의 Zr/Ti 비에 따른 전기적 특성과 공진주파수의 온도안정성)

  • 류주현;윤광희;민석규;이명수;서성재
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.13 no.8
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    • pp.675-680
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    • 2000
  • In this study the temperature coefficient of resonant frequency(TC $F_{r}$) dielectric and piezoelectric properties of Pb[(S $b_{1}$2//N $b_{1}$2/)$_{0.065}$)-(Z $r_{x}$, $Ti_{1-x}$ )$_{0.90}$] $O_3$ceramics were investigated with Zr/Ti ratio. The compositions near the morphotropic phase boundary (MPB) appeared when Zr/Ti ratio was 49.5/50.5 The dielectric constant and electromechanical coupling factor( $k_{p}$) also showed the highest values of 1,257, 0.653 respectively when the Zr/Ti ratio was 49.5/50.5 Moreover the mechanical quality factor( $Q_{m}$) showed th lowest value of 713 when the Zr/Ti ratio. The temperature coefficient of resonant frequency(TC $F_{r}$) abruptly change at the morphotropic phase boundary(MPB) which existed between the rhombohedral phase with highly negative TC $F_{r}$ of -106ppm/$^{\circ}C$ and the tetragonal phase with highly positive TC $F_{r}$ of +64pp $m^{\circ}C$ as Zr/Ti ratio varied from 50/50 to 49.5/50.5.50.5..50.5.

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