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Rapid Detection Method for Fusaric Acid-producing Species of Fusarium by PCR (후자린산(Fusaric acid) 생성 Fusarium 종의 신속 검출 PCR)

  • Lee, Theresa;Kim, Sosoo;Busman, Mark;Proctor, Robert H.;Ham, Hyeonhui;Lee, Soohyung;Hong, Sung Kee;Ryu, Jae-Gee
    • Research in Plant Disease
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    • v.21 no.4
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    • pp.326-329
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    • 2015
  • Fusaric acid is a mycotoxin produced by species of the fungus Fusarium and can act synergistically with other Fusarium toxins. In order to develop a specific detection method for fusaric acid-producing fungus, PCR primers were designed to amplify FUB10, a transcription factor gene in fusaric acid biosynthetic gene cluster. When PCR with Fub10-f and Fub10-r was performed, a single band (~550 bp) was amplified from F. oxysporum, F. proliferatum, F. verticillioides, F. anthophilum, F. bulbicola, F. circinatum, F. fujikuroi, F. redolens, F. sacchari, F. subglutinans, and F. thapsinum, all of which were known for fusaric acid production. Whereas the FUB10 specific band was not amplified from Fusarium species known to be trichothecene producer. Because production of fusaric acid can co-occur in species that also produce fumonisin mycotoxins, we developed a multiplex PCR assay using the FUB10 primers as well as primers for the fumonisin biosynthetic gene FUM1. The assay yielded amplicons from fumonisin producers such as F. proliferatum and F. verticillioides, allowing for the simultaneous detection of species with the genetic potential to produce both types of mycotoxins.

A Study on Shoulder Joint ROM of the Elderly (노인의 견관절 가동범위에 관한 연구)

  • Um, Ki-Mai;Yang, Yoon-Kwon
    • Journal of Korean Physical Therapy Science
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    • v.8 no.2
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    • pp.997-1003
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    • 2001
  • The purpose of this study is to know the average of pint range of motion and difference according to the aging for the elderly, This study consisted of elder male(n=75) and elder female(n=l09), The result of assessment and analysis in shoulder pint range of motion are as follows: 1) The average shoulder flexion pint range of motion in 60-69(from sixty to sixty-nine)years old are 163.04(Left-Male), 162.91(Right-Male), 158.74 (Left-Female), 158.74 (Right-Female). 70-79years old are 149.40(L-M), 152.38(R-M), 153,37(L-F), 153.37(R-F). 80-89 years old are 149.57(L-M), 147.93(R-M), 151.17(L-F), 150.33(R-F). There was no significant difference among group, 2) The average shoulder extension pint range of motion in 60-69years old are 48.15(L-M), 47.20(R-M), 45.16(L-F), 44.23(R-F), 70-79years old are 37.l1(L-M), 38.70(R-M), 35.17(L-F), 36.71(R-F), 80-89 years old are 34.46(L-M). 36.71(R-M), 33.90(L-F), 33.09(R-F). There was significant difference among group(p<.05). 3) The average shoulder abduction pint range of motion in 60-69years old are 164.22(L-M), 165.96(R-M), 159.34(L-F), 159.97(R-F), 70-79years old are 152.27(L-M), 155.05(R-M), 152.32(L-F), 53.66(R-F), 80-89 years old are 152.17(L-M), 153.76(R-M), 147.53(L-F), 147.37(R-F). There was significant difference in right shoulder abduction among group(p<05). 4) The average shoulder internal rotation pint range of motion in 60-69years old are 63.52(L-M), 65.70(R-M), 64.16(L-F), 64.61(R-F), 70-79years old are 64.50(L-M), 65.81(R-M) 61.10(L-F), 61.83(R-F). 80-89 years old are 61.60(L-M), 61.66(R-M), 57.53(L-F), 57.53(R-F). There was no significant difference among group. 5) The average shoulder external rotation pint range of motion in 60-69years old are 50.87(L-M), 50.22(R-M), 51.03(L-F), 50.42(R-F), 70-79years old are 50.91(L-M), 50.20(R-M) 48.37(L-F), 50.20(R-F). 80-89 years old are 46.83(L-M), 47.93(R-M), 43.43(L-F), 43.72(R-F).There was significant difference in left shoulder external rotation among group(p<.05).

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IDEMPOTENCE PRESERVING MAPS ON SPACES OF TRIANGULAR MATRICES

  • Sheng, Yu-Qiu;Zheng, Bao-Dong;Zhang, Xian
    • Journal of applied mathematics & informatics
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    • v.25 no.1_2
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    • pp.17-33
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    • 2007
  • Suppose F is an arbitrary field. Let ${\mid}F{\mid}$ be the number of the elements of F. Let $T_{n}(F)$ be the space of all $n{\times}n$ upper-triangular matrices over F. A map ${\Psi}\;:\;T_{n}(F)\;{\rightarrow}\;T_{n}(F)$ is said to preserve idempotence if $A-{\lambda}B$ is idempotent if and only if ${\Psi}(A)-{\lambda}{\Psi}(B)$ is idempotent for any $A,\;B\;{\in}\;T_{n}(F)$ and ${\lambda}\;{\in}\;F$. It is shown that: when the characteristic of F is not 2, ${\mid}F{\mid}\;>\;3$ and $n\;{\geq}\;3,\;{\Psi}\;:\;T_{n}(F)\;{\rightarrow}\;T_{n}(F)$ is a map preserving idempotence if and only if there exists an invertible matrix $P\;{\in}\;T_{n}(F)$ such that either ${\Phi}(A)\;=\;PAP^{-1}$ for every $A\;{\in}\;T_{n}(F)\;or\;{\Psi}(A)=PJA^{t}JP^{-1}$ for every $P\;{\in}\;T_{n}(F)$, where $J\;=\;{\sum}^{n}_{i-1}\;E_{i,n+1-i}\;and\;E_{ij}$ is the matrix with 1 in the (i,j)th entry and 0 elsewhere.

INVOLUTION-PRESERVING MAPS WITHOUT THE LINEARITY ASSUMPTION AND ITS APPLICATION

  • Xu, Jin-Li;Cao, Chong-Guang;Wu, Hai-Yan
    • Journal of applied mathematics & informatics
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    • v.27 no.1_2
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    • pp.97-103
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    • 2009
  • Suppose F is a field of characteristic not 2 and $F\;{\neq}\;Z_3$. Let $M_n(F)$ be the linear space of all $n{\times}n$ matrices over F, and let ${\Gamma}_n(F)$ be the subset of $M_n(F)$ consisting of all $n{\times}n$ involutory matrices. We denote by ${\Phi}_n(F)$ the set of all maps from $M_n(F)$ to itself satisfying A - ${\lambda}B{\in}{\Gamma}_n(F)$ if and only if ${\phi}(A)$ - ${\lambda}{\phi}(B){\in}{\Gamma}_n(F)$ for every A, $B{\in}M_n(F)$ and ${\lambda}{\in}F$. It was showed that ${\phi}{\in}{\Phi}_n(F)$ if and only if there exist an invertible matrix $P{\in}M_n(F)$ and an involutory element ${\varepsilon}$ such that either ${\phi}(A)={\varepsilon}PAP^{-1}$ for every $A{\in}M_n(F)$ or ${\phi}(A)={\varepsilon}PA^{T}P^{-1}$ for every $A{\in}M_n(F)$. As an application, the maps preserving inverses of matrces also are characterized.

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SOME GENERALIZATION OF THE LANG'S EXISTENCE OF RATIONAL PLACE THEOREM

  • Cho, In-Ho;Lim, Jong-In
    • Bulletin of the Korean Mathematical Society
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    • v.22 no.2
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    • pp.83-85
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    • 1985
  • Let K be a real function field over a real closed field F. Then there exists an F-place .phi.:K.rarw.F.cup.{.inf.}. This is Lang's Existence of Rational Place Theorem (6). There is an equivalent version of Lang's Theorem in (4). That is, if K is a function field over a field F, then, for any ordering P$_{0}$ on F which extends to K, there exists an F-place .phi.: K.rarw.F'.cup.{.inf.} where F' is a real closure of (F, P$_{0}$). In [2], Knebusch pointed out the converse of the version of Lang's Theorem is also true. By a valuation theoretic approach to Lang's Theorem, we have found out the following generalization of Lang and Knebusch's Theorem. Let K be an arbitrary extension field of a field F. then an ordering P$_{0}$ on F can be extended to an ordering P on K if there exists an F-place of K into some real closed field R containing F. Of course R$^{2}$.cap.F=P$_{0}$. The restriction K being a function field of F is vanished, though the codomain of the F-place is slightly varied. Therefore our theorem is a generalization of Lang and Knebusch's theorem.

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On a Question of Closed Maps of S. Lin

  • Chen, Huaipeng
    • Kyungpook Mathematical Journal
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    • v.50 no.4
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    • pp.537-543
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    • 2010
  • Let X be a regular $T_1$-space such that each single point set is a $G_{\delta}$ set. Denot 'hereditarily closure-preserving' by 'HCP'. To consider a question of closed maps of S. Lin in [6], we improve some results of Foged in [1], and prove the following propositions. Proposition 1. $D\;=\;\{x{\in}X\;:\;\mid\{F{\in}\cal{F}:x{\in}F\}\mid{\geq}{\aleph}_0\}$ is discrete and closed if $\cal{F}$ is a collection of HCP. Proposition 2. $\cal{H}\;=\;\{{\cup}\cal{F}'\;:\;F'$ is an fininte subcolletion of $\cal{F}_n\}$ is HCP if $\cal{F}$ is a collection of HCP. Proposition 3. Let (X,$\tau$) have a $\sigma$-HCP k-network. Then (X,$\tau$) has a $\sigma$-HCP k-network F = ${\cup}_n\cal{F}_n$ such that such tat: (i) $\cal{F}_n\;\subset\;\cal{F}_{n+1}$, (ii) $D_n\;=\;\{x{\in}X\;:\;\mid\{F{\in}\cal{F}_n\;:\;x{\in}F\}\mid\;{\geq}\;{\aleph}_0\}$ is a discrete closed set and (iii) each $\cal{F}_n$ is closed to finite intersections.

Soil-borne Diseases of Barley in Barley in Korea Caused by Fusarium spp. (한국에서의 Fusarium균에 의한 보리의 토양전염성병)

  • Sung Jae Mo;Snyder William C.;Chung Bong Koo;Chung Bong Jo
    • Korean journal of applied entomology
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    • v.16 no.2 s.31
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    • pp.115-119
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    • 1977
  • Fusarium spp. were isolated from field grown rice, wheat and barley in 1976. The pathogens isolated included Fusarium (Calonectria) nivale, F. (Gibberella) moniliforme and F. (Gibberella) roseum 'Graminearum' and 'Avenaceum'. Among the saprophytes F. (Nectria) episphaeria was isolated. In each of these isolated both the Fusarium and perfect stages were found. F. nivale, and F. episphaeria with there Calonectria, and Nectria stages do not seem to have been recorded previously in Korea. Of the Fusaria isolated, $66.3\%$ from rice were F. moniliforme, and $68.2\%$ from wheat and barley were F. roseum 'Graminearum'. Perithecia also were produced under laboratory conditions. F. moniiforme was recovered wheat heads and also from barley seed.

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Chromosomal Study on the Genus Fusarium (Fusarium속의 염색체에 관한 연구)

  • Min, Byung-Re
    • The Korean Journal of Mycology
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    • v.18 no.3
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    • pp.132-136
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    • 1990
  • The vegetative nuclear divisions in hyphae and the chromosome of Fusarium were observed by use of HCI-Giemsa technique and light microscope. The chromosome of nuclear in F. moniliforme both 7150 and 7219 were eight. F. subglutinans 1082 was n=8 and n=7 in F. sub­glutinans 1083. F. nygamai 5668 was n=7 and n=5 in F. nygamai 7132. F. beomiforme 9758 and 9760 were n=7. F. coccidicola ATCC 24138 and F. acuminatum ATCC 16560 were n=6. From these results and other reports, the basic chromosomal number of these fungi might be speculated to be four.

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Fusarium Species from Sorghum in Thailand

  • Mohamed Nor, Nik M.I.;Salleh, Baharuddin;Leslie, John F.
    • The Plant Pathology Journal
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    • v.35 no.4
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    • pp.301-312
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    • 2019
  • Sorghum is the fifth most important cereal worldwide, spreading from Africa throughout the world. It is particularly important in the semi-arid tropics due to its drought tolerance, and when cultivated in Southeast Asia commonly occurs as a second crop during the dry season. We recovered Fusarium from sorghum in Thailand and found F. proliferatum, F. thapsinum and F. verticillioides most frequently, and intermittent isolates of F. sacchari and F. beomiforme. The relatively high frequencies of F. proliferatum and F. verticillioides, suggest mycotoxin contamination, particularly fumonisins and moniliformin, should be evaluated. Genetic variation within the three commonly recovered species was characterized with vegetative compatibility, mating type, Amplified Fragment Length Polymorphisms (AFLPs), and female fertility. Effective population number ($N_e$) was highest for F. verticillioides and lowest for F. thapsinum with values based on mating type allele frequencies higher than those based on female fertility. Based on AFLP genetic variation, the F. thapsinum populations were the most closely related, the F. verticillioides populations were the most distantly related, and the F. proliferatum populations were in an intermediate position. The genetic variation observed could result if F. thapsinum is introduced primarily with seed, while F. proliferatum and F. verticillioides could arrive with seed or be carried over from previous crops, e.g., rice or maize, which sorghum is following. Confirmation of species transmission patterns is needed to understand the agricultural systems in which sorghum is grown in Southeast Asia, which are quite different from the systems found in Africa, Australia, India and the Americas.

GENERALIZED HYERS-ULAM STABILITY OF FUNCTIONAL EQUATIONS

  • Kwon, Young Hak;Lee, Ho Min;Sim, Jeong Soo;Yang, Jeha;Park, Choonkil
    • Journal of the Chungcheong Mathematical Society
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    • v.20 no.4
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    • pp.387-399
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    • 2007
  • In this paper, we prove the generalized Hyers-Ulam stability of the following linear functional equations f(x + iy) + f(x - iy) + f(y + ix) + f(y - ix) = 2f(x) + 2f(y) and f((1 + i)x) = (1 + i)f(x), and of the following quadratic functional equations f(x + iy) + f(x - iy) + f(y + ix) + f(y - ix) = 0 and f((1 + i)x) = 2if(x) in complex Banach spaces.

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