• Membrane and Water Treatment
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• v.7 no.3
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• pp.223-240
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• 2016

In this study, poly(vinyl-alcohol) and water insoluble ${\beta}$-cyclodextrin polymer (${\beta}$-CDP) cross-linked with citric acid, have been used as macrocyclic carrier in the preparation of polymer inclusion membranes (PIMs) for aniline (as molecule model) extraction from aqueous media. The obtained membranes were firstly characterized by X-ray diffraction, Fourier transform infrared and water swelling test. The transport of aniline was studied in a two-compartment transport cell under various experimental conditions, such as carrier content in the membranes, stirring rate and initial aniline concentration. The kinetic study was performed and the kinetic parameters were calculated as rate constant (k), permeability coefficient (P) and flux (J). These first results demonstrated the utility of such polymeric membranes for environmental decontamination of toxic organic molecules like aniline. Predictive modeling of transport flux through these materials was then studied using design of experiments; the design chosen was a two level full factorial design $2^k$. An empirical correlation between aniline transport flux and independent variables (Poly ${\beta}$-CD membrane content, agitation speed and initial aniline concentration) was successfully obtained. Statistical analysis showed that initial aniline concentration of the solution was the most important parameter in the study domain. The model revealed the existence of a strong interaction between the Poly ${\beta}$-CD membrane content and the stirring speed of the source solution. The good agreement between the model and the experimental transport data confirms the model's validity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.3
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• pp.223-229
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• 2015

In this study, we investigated the effect of ethanol extracts from Gentianae scabrae bunge (GSB) on the activities of antioxidant, whitening and anti-inflammation. Viability of cells was measured by neutral red (NR) assay, and inhibitory effects of GSB on melanin synthesis was determined the melanin production in B16F10 cells. The expression level of matrix metalloproteinase-1 (MMP-1) in media was analyzed by ELISA kit, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. GSB showed highly efficacy in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and significantly reduced melanin synthesis in B16F10 cells. MMP-1 production in UVB-stimulated human dermal fibroblast (HDF) cells was inhibited by GSB treatments. NO production was suppressed by the treatment of GSB in LPS-stimulated RAW264.7 cells. From this results, it was indicated that GSB could be utilized as anti-aging and whitening cosmetic ingredients.

• Korea journal of population studies
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• v.11 no.1
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• pp.87-96
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• 1988

The subjects used in this study were chosen from self-employed women in Taegu City, Korea, Data for 415 respondents were collected by face-to-face interviews conducted during the period of November 10 through 23, 1987. There are three major purposes in this study : first, to describe the general characteristics of self-employed Korean women's fertility behavior ; second, to examine the determinants of fertility behavior among self-employed women : third, to explore the relative importance of the fertility determinants among the women. Regression analyses were introduced to test hypotheses. Nine variables, such as household income, educational attainment, mass media, abortion, the period of contraception practice, contraceptive expenses, the number of infant, deaths marital period, and age at first marriage were introduced as indicators in the regression. The results of this study show that there are negative relationships between the dependent variable of fertility and abortion, and the period of contraception practice. On the other hand, positive relationships between the dependent variable of fertility and the number of infant deaths, and marital period and age at first marriage are shown in this study. However, the results of this study show that there are insignificant relationships between socioeconomic variables and fertility. From the results of the F test to evaluate the significance of difference in $R^2$between two different regression equations, we have found that the regression equation including both socioeconomic variables and intermediate variables is misspecified to examine the determinants of fertility behavior among self-employed Korean women. That is, the F test shows that the regression equation including only intermediate variables as indicators is the best model for this study. Finally, the relative importance of the fertility determinants among self-employed women is found as follows : Age at the first marriage is the most influential variable in the fertility, and the next important factors are abortion, the period of contraception practice, the number of infant deaths, and marital period, in that order.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.62-62
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• 2023

강우가 지표면 아래로 침투할 때 초기에는 투수층이 불포화 상태이어서 부압이 작용하면서 침투할 것이다. Richards 식(Richards, 1931)을 써서 불포화 투수층의 침투를 모의할 수 있다. 강우가 지속되는 동안 하상 아래 어느 구간은 포화 상태가 되어 Richards 식을 더 이상 사용할 수 없다. 하지만 현재까지의 연구는 Richards 식을 사용하여 침투를 모의하는 오류를 범하고 있다. 강우에 의한 침투를 예측할 때 지표면에서의 침투율 q_b 가 필요한 데 현존하는 연구에서는 Horton 식(Horton, 1941)을 사용하여 초기 침투율 f_o 와 장시간 후 침투율 f_c 와 시간에 따라 지수함수로 감소하는 계수 k 의 3가지 계수값을 실험이나 현장 관측값에서 찾아서 쓰고 있다. 그런데, 이 계수값은 강우강도 r_i 가 클수록 침투율 q 가 커지는 물리 현상을 반영하지 못하는 한계가 있다. 본 연구에서 먼저 포화 투수층에서의 침투를 모의하는 식을 개발하였다. 지표면 아래에서 불포화 투수층에는 Richards식을 사용하고 포화 투수층에는 개발한 식을 사용하여 침투를 모의하였다. 또한 지표면에서의 침투율 q_b 를 구하는 공식을 개발하였다. 하상에서의 침투율의 최대값은 $q_{bmax}=-{\lambda}{\sqrt{2g(s-b)}}$ 일 것이다. 여기서 λ 는 투수층의 공극율, s 는 유출수면의 위치, b 는 지표면의 위치이다. 지표면에서의 침투율의 최소값 q_bmin 은 지표면 바로 아래 지점에서의 침투율일 것이다. 지표면에서의 침투율 q_b 로 q_bmax 와 q_bmin 사이의 적절한 값을 선택한다. 강우강도를 r_i 라고 하면 지표면 위 유출수의 연속방정식은 다음과 같다: $s-b={\int}(r_i-{\mid}q_b{\mid})dt$. 즉, 유출수면의 위치 s 는 강우강도 r_i 가 클수록 또는 지표면에서의 유출율의 크기 |q_b| 가 작을수록 크다. 또한 지표면에서의 침투율 q_b 와 지표면 아래에서의 침투율 q 는 s - b 가 클수록 크다. 따라서, 강우강도 r_i 가 클수록 침투율 q_b, q 가 큰 현상이 잘 반영되었다. 강우-침투-유출 모형실험을 수행하여 강우강도에 따라 침투율과 유출량이 다른 현상을 관측하여 수치실험 결과와 비교·검증하였다.

• Korean journal of applied entomology
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• v.13 no.2 s.19
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• pp.93-97
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• 1974

This paper describes experiments made to develop a technique for marking insects and get the informations for ecological studies as a means of tagging flies with radioactive phosphorus, and also to learn the effect on the subsequent generation of flies. Tests were conducted with house flies, Musca domestica L. emerged front the larvae that had reared in a media containing various concentrations of P-32. Radioactivity of the flies was measured with end-window type $(2mg/cm^2)$ Geiger-Muller counter and proportional counter made by Aloka. In following the daily increases in counting rates by 6-day-old larvae it was apparent the larvae in the starting media containing the higher concentrations of P-32 were showing high counting rates. It was found that about 13.2 per cent of the radioactive material remained in the empty puparia after the flies emerged. Radioactivity of the emerged flies decreased gradually and the biological halflife of phosphorus-32 was approximately 6.14 days with considerable variation. A significant amount of P-32 were detected in the abdominal and thoracic sections as compared with other parts of adult. The activity of phosphorus·32 in eggs and larvae produced by adults reared in medium containing 0.2 microcurie or more of P-32 per gram were readily detected with Geiger-Mi·lier counter. On the other hand, the radioactivity of eggs and larvae produced by the treated males crossed with untreated normal females could be only detected in the medium containing more of 2.0 microcurie with G.M. counter. There was no apparent damage to the flies at dosages as high as 0.5 microcurie per gram of medium. For field studies a concentration of 0.5 microcurie of P-32 per gram of artificial medium appears to give adequate labeling for positive identification of flies and their egg masses.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.287-292
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• 2002

Lignin-peroxidase (LiP) has been considered as one of the most important industrial enzymes for biodegradation of various recalcitrant toxic compounds such as chlorinated aromatic hydrocarbons and azo-dyes. Recently, several soil actinomycetes have been reported to secrete a functionally-similar lignin-peroxidase called actinomycetes lig-nin-peroxidase (ALiP). In this manuscript, we isolated over 100 morphologically distinct actinomycetes from the contaminated soils around 10 different gas stations located nearby the Kyung-An river. Among these actinomycetes screened based on the congo-red dye-decolorization activities, one newly-isolated actinomycetes named SMA-2 showed the most significant dye-decoloring activity on the congo-red plate as well as a significant ALiP activity in a yeast-extract-malt-extract liquid media supplemented with starch. The optimum SMA-2 culture condition fur ALiP production was determined and the kinetic parameters fur the SMA-2 AkIP activity were characterized. The optimally-cultured SMA-2 also exhibited the oxidation activities toward various recalcitrant aromatic compounds including phenol, 2- chlorophenol, 4- chlorophenol, 2,4- dichlorophenol ,2,6- dichlorophenol, and 2,4, f-trichlorophe - not, suggesting a potential application of SMA-2 for contaminated soil bioremediation.

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.461-466
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• 1982

Lower and upper petiole segments of strawberry plants (Fragaria${\times}$ananassa) were cultured on Murashige and Skoog (MS) medium containing 2mg/liter Benzylamino purine (BA), 0.02mg/liter 2,4-dichlorophenoxyacetic acid (2,4-D), or their coinbination. Of the lower petiole segments, 55% or more were differentiated on the media containing $2mg/{\ell}\;BA$ or $2mg/{\ell}\;BA+0.02mg/{\ell}\;2,4-D$. There was no significant difference in results be tween these treatments. BA did not influence root formation. No shoots we re initiated from the upper petiole segments. Root formation was stimulated by the $0.02mg/{\ell}\;2,4-D$ medium, whereas it inhibited shoot formation. Upper petiole segments produced more roots than did lower petiole segments.

• Microbiology and Biotechnology Letters
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• v.29 no.2
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• pp.72-77
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• 2001

Expression of f3 ~agarase Gene and Catabolite Repression in Escherichia coli by the Promoter of Alginate Lyase Gene Isolated from Marine Pseudomonas sp. Jin, Cheal~Ho, J~Hyeon Park, Jeong-Hyun Han, YoonM Hyeok Chae, Jong~Hee Lee, Jung-Kee Lee!, and In-800 Kong*. Faculty of Food Science and Biotechnology, Pukyong National UniversitYt Pusan 608-737, Korea, llnBioNet Co. 1690-3 Taejon 306-230, Korea - Promoter is a key factor for expression of the recombinant protein. There are many promoters for overexpression of protein in various organisms. The aly promoter of Pseudomonas sp. W7 isolated from marine environment was known to be a constitutive expression promoter of the alginate lyase gene, and it's promoter activity is repressed by glucose in Escherichia coli. To investigate the catabolite repression of the aly promoter ~md association between the promoter mutants, f3 agarase gene, which was also cloned from Pseudomonas sp. W7 was connected to the aly promoter with the sequence the coding 46 N-terminal amino acids ofthe alginate lyase gene. The constructed plasmid was introduced into E. coli and the agarase activity was measured. Fourty six amino acids of the alginate lyase gene was serially deleted using peR to the direction of 5' upstream region and subcloned. The agarase was overexpressed by the aly promoter and the production of agarase was repressed by the addition of glucose into culture media. Fourty six amino acids of alginate lyase did not affect the production of agarase at all. The deletion of a putative stem-loop structure in the aly promoter induced the decrease of f3 -agarase productivity.

• The Journal of the Korea Contents Association
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• v.14 no.7
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• pp.45-57
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• 2014

This study examines subjects' eye movement pattern and surveys their attitudes to the exposed advertisements on the Facebook. Different from the F-shaped pattern of the typical Web pages, users' eye movements on the Facebook have shown a rough H-shape. Even though a large number of users have shown F-shaped pattern on the ordinary Web pages in order to skip the contents of a Web, subjects' eye-movement pattern on the Facebook has H -shaped pattern due to the unique User Interface (UI) of the Facebook. With the right side and vertical arrangement of ads on the Facebook, users skip the page with having a large H-shaped pattern. In addition, this study set four AOIs(Area of Interest) that are advertising sections comprised on the Facebook Web page and measured fixation length within the AOIs then surveyed subjects' attitudes about the exposed ads. Through the experiment and survey, this study offers the optimum advertising position that can attract Facebook users' attention. As the result of experiment and survey, the second ad has the subjects' highest attitude to advertising and fourth ad is the next effectiveness and first and third ad followed. This study highlights the key implications to provide better user experiences(UX) and marketing strategies to users who are the consumers of companies and organizations which have a plan to put their advertising on the Facebook.

• Microbiology and Biotechnology Letters
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• v.34 no.3
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• pp.228-234
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• 2006

The culture conditions were investigated to maximize the production of laccase from Fomitella fraxinea mycelia. Among the tested media, mushroom complete medium (MCM) showed the highest production of the enzyme. The optimum culture medium was 2% dextrose, 0.4% $(NH_4)_{2}HPO_4$, 0.05% $Na_{2}HPO_{4}{\cdot}7H_{2}O$, and 0.05% KCl as carbon, nitrogen, phosphorus, and inorganic salt sources respectively. SDS-PAGE followed by laccase activity staining using 2,6-djmethoxyphenol as the substrate was performed to identify the laccase activity under culture conditions studied. Zymogram analysis of the culture supernatant showed a laccase band with a molecular mass of 50 kDa. The enzyme production from F. fraxinea was reached to the highest level after the cultivation for 10 days at $25^{\circ}C$ and initial pH 8. The enzyme activity of the culture supernatant was most active at $50^{\circ}C$ and pH 5.