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http://dx.doi.org/10.15188/kjopp.2015.06.29.3.223

Study on Pharmacological Activation as Cosmetic Material of Gentianae scabrae bunge Extract  

Choi, Hyung Wook (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
In, Myung Hee (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
Mun, Yeun Ja (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
Lim, Kyu Sang (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University)
Woo, Won Hong (Research Center of Traditional Korean Medicine)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.29, no.3, 2015 , pp. 223-229 More about this Journal
Abstract
In this study, we investigated the effect of ethanol extracts from Gentianae scabrae bunge (GSB) on the activities of antioxidant, whitening and anti-inflammation. Viability of cells was measured by neutral red (NR) assay, and inhibitory effects of GSB on melanin synthesis was determined the melanin production in B16F10 cells. The expression level of matrix metalloproteinase-1 (MMP-1) in media was analyzed by ELISA kit, and nitric oxide (NO) production in RAW264.7 cells was monitored by measuring the nitrite content in culture medium. GSB showed highly efficacy in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and significantly reduced melanin synthesis in B16F10 cells. MMP-1 production in UVB-stimulated human dermal fibroblast (HDF) cells was inhibited by GSB treatments. NO production was suppressed by the treatment of GSB in LPS-stimulated RAW264.7 cells. From this results, it was indicated that GSB could be utilized as anti-aging and whitening cosmetic ingredients.
Keywords
Gentianae scabrae bunge; antioxidant; melanin; MMP-1; nitric oxide;
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