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Optimal Production and Characterization of Laccase from Fomitella fraxinea Mycelia  

Park Kyung-Mi (Department of Biotechnology, Dongguk University)
Park Sang-Shin (Department of Biotechnology, Dongguk University)
Publication Information
Microbiology and Biotechnology Letters / v.34, no.3, 2006 , pp. 228-234 More about this Journal
Abstract
The culture conditions were investigated to maximize the production of laccase from Fomitella fraxinea mycelia. Among the tested media, mushroom complete medium (MCM) showed the highest production of the enzyme. The optimum culture medium was 2% dextrose, 0.4% $(NH_4)_{2}HPO_4$, 0.05% $Na_{2}HPO_{4}{\cdot}7H_{2}O$, and 0.05% KCl as carbon, nitrogen, phosphorus, and inorganic salt sources respectively. SDS-PAGE followed by laccase activity staining using 2,6-djmethoxyphenol as the substrate was performed to identify the laccase activity under culture conditions studied. Zymogram analysis of the culture supernatant showed a laccase band with a molecular mass of 50 kDa. The enzyme production from F. fraxinea was reached to the highest level after the cultivation for 10 days at $25^{\circ}C$ and initial pH 8. The enzyme activity of the culture supernatant was most active at $50^{\circ}C$ and pH 5.
Keywords
Fomitella fraxinea; laccase; optimal production;
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1 Das, N., S. Sengupta, and M. Mukhrjee. 1997. Importance of laccase in vegetative growth of Pleurotus florida. Appl. Environ. Microbiol. 63: 4120-4122
2 de Souza, C. G. M., G. K. Tychanowicz, D. F. Souza, and R. M. Peralta. 2004. Production of laccase isoforms by Pleurotus pulmonarius in response to presence of phenolic and aromatic compounds. J. Basic Microbiol. 44: 129-136   DOI   ScienceOn
3 Fukuda, T., H. Uchida, Y. Takashima, T. Uwajima, T. Kawabata, and M. Suzuki. 2001. Degradation of bisphenol A by purified laccase from Trametes villosa. Biochem. Biophys. Res. Commun. 284: 704-706   DOI   ScienceOn
4 Leontievsky, A. A., N. M. Myasoedova, B. P. Baskunov, C. S. Evans, and L. A. Golovleva. 2000. Transformation of 2,4,6-trichlorophenol by the white-rot fungi Panus tigrinus and Coriolus versicolor. Biodegradation 11: 331-340   DOI   ScienceOn
5 Salony, S. Mishra, and V. S. Bisaria. 2006. Production and characterization of laccase from Cyathus bulleri and its use in decolourization of recalcitrant textile dyes. Appl. Microbiol. Biotechnol. 71: 646-653   DOI
6 Solano, F., P. Lucas-Elio, D. Lopez-Serrano, E. Ferandez, and A. Sanchez. 2001. Dimethoxyphenol oxidase activity of different microbial blue multicopper proteins. FEMS Microbiol. 204: 175-181   DOI
7 Ullrich, R., M. Huong Le, N. L. Dung, and M. Hofrichter. 2005. Laccase from the medicinal mushroom Agaricus blazei: production, purification and characterization. Appl. Microbiol. Biotechnol. 67: 357-363   DOI
8 Wang, J. W., J. H. Wu, W. Y. Huang, and R. X. Tan. 2005. Laccase production by Monotospora sp., an endophytic fungus in Cynodon dactylon. Bioresource Technol. 97: 786-789   DOI   ScienceOn
9 Papinutti, V. L. and F. Forchiassin. 2003. Optimization of manganese peroxidase and laccase production in the South American fungus Fomes sclerodermeus (Lev.) Cke. J. Ind. Microbiol. Biotechnol. 30: 536-541   DOI
10 Arora, D. S. and P. K. Gill. 2000. Laccase production by some white rot fungi under different nutritional conditions. Bioresource Technol. 73: 283-285   DOI   ScienceOn
11 Park, S. S., J. S. Lee, K. G. Bae, K. H. Yu, H. C. Han, and T. J. Min. 2001. Antioxidative activity and structural Analysis of the steroid compound from Fomitella fraxinea. Kor. J. Mycol. 29: 67-71
12 Hou, H., J. Zhou, J. Wang, C. Dua, and B. Yan. 2004. Enhancement of laccase production by Pleurotus ostreatus and its use for the decolorization of anthraquinone dye. Process Biochem. 39: 1415-1419   DOI   ScienceOn
13 Prasad, K. K., S. V. Mohan, Y. V. Bhaskar, S. V. Ramanaiah, V. L. Babu, and P. N. Sarma. 1804. Laccase production using Pleurotus ostreatus immobilized on PUF cubes in batch and packed bed reactors: Influence of culture conditions. J. Microbiol. 43: 301-307
14 Lee, J. S., H. S. Baik, and S. S. Park. 2006. Purification and Characterization of two novel fibrinolytic proteases from mushroom, Fomitella fraxinea. J. Microbiol. Biotechnol. 16: 262-271   과학기술학회마을
15 Solano, F., E. Garcia, D. Perez, and A. Schez-Amat. 1997. Isolation and characterization of strain MMB-1 (CECT 4803), a novel melanogenic marine bacterium. Appl. Environ. Microbiol. 63: 3499-3506
16 Wolf, S. L. 1993. Molecular and Cellular Biology, Wandsworth, Inc., California. pp. 287-292
17 Park, S. S. and S. M. Hwang. 1999. Purification and characterization of a iron-containing superoxide dismutase from Lentinus edodes. J. Microbiol. Biotechnol. 9: 854-860
18 Perez, J., J. Martinez, and T. de la Rubia. 1996. Purification and partial characterization of a laccase from the white rot fungus Phanerochaete favido-alba Appl. Environ. Microbiol. 62: 4263-4267
19 Solomon, E. I., U. M. Sundaram, and T. E. Machonkin. 1996. Multicopper oxidase and oxygenases. Chem. Rev. 96: 2563-2605   DOI   ScienceOn
20 Lee, J. H., S. M. Cho, H. M. Kim, N. D. Hong, and I. D. Yoo. 1997. Immunostimulating activity of polysaccharides from mycelia of Phellinus linteus grown under different culture conditions. J. Microbiol. Biotechnol. 7: 52-55
21 Xiao, Y. Z., X. M. Tu, J. Wang, M. Zhang, Q. Cheng, W. Y. Zeng, and Y. Y. Shi. 2003. Purification, molecular characterization and reactivity with aromatic compounds of a laccase from basidiomycete Trametes sp. strain AH28-2. Appl. Microbiol. Biotechnol. 60: 700-707
22 Nonaka, T., H. Ishikawa, Y. Tsumuraya, Y. Hashimoto, and N. Dohmae. 1995. Characterization of a thermostable lysine-specific metallopeptidase from the fruiting bodies of a basidiomycete, Grifola frondosa. J. Biochem. (Tokyo) 118: 1014-1020
23 Staib, F., M. Seibold, E. Antweiler, B. Frohlich, S. Weber, and A. Blisse. 1987. The brown colour effect (BCE) of Cryptococcus neoformans in the diagnosis, control and epidemiology of C. neoformans in AIDS Patients. Zentralbl. Bakteriol. Mikrobiol. Hyg. [A] 266: 167-177
24 Vasconcelos, A. F. D., A. M. Barbosa, R. F. H. Dekker, I. S. Scarminio, and M. I. Rezende. 2000. Optimizaton of laccase production by Botryosphaeria sp. in the presence of veratryl alcohol by the response-surface method. Process Biochem. 35: 1131-1138   DOI   ScienceOn
25 Mikiashvili, N., V. Elisashvili, S. Wasser, and E. Nevo. 2005. Carbon and nitrogen sources influence the ligninolytic enzyme activity of Trametes versicolor. Biotechnol. Lett. 27: 955-959   DOI
26 Ullah, M. A., C. T. Bedford, and C. S. Evans. 2000. Reactions of pentachlorophenol with laccase from Coriolus Versicolor. Appl. Microbiol. Biotechnol. 53: 230-234   DOI
27 Call, H. P. and I. Mucke. 1997. History, overview and applications of mediated lignolytic systems, especially laccase-mediator-systems (Lignozymprocess). J. Biotechnol. 53: 163-202   DOI   ScienceOn
28 Niku-Paavola, M. -L., and L. Viikari. 2000. Enzymatic oxidation of alkenes. J. mol. Gatal., B Enzym. 10: 435-444   DOI   ScienceOn
29 Heinzkill., M. L. Bech, T. Halkiler, P. Schneider, and T. Anke. 1998. Characterization of laccases and peroxidases from wood-rotting fungi (family Coprinaceae). Appl. Environ. Microbiol. 64: 1601-1606
30 Perry, C. R., M. Smith, C. H. Britnell, D. A. Wood, and C. F. Thurston. 1993. Identification of two laccase genes in the cultivated mushroom Agaricus bisporus. J. Gen. Microbiol. 139: 1209-1218
31 Dong, J. L., Y. W. Zhang, R. H. Zhang, W. Z. Huang, and Y. Z. Zhang. 2005. Influence of culture conditions on laccase production and isozyme patterns in the white-rot fungus Trametes gallica. J. Basic Microbiol. 45: 190-198   DOI   ScienceOn
32 Garzillo, A. M. V., and M. C. Colao. 1998. Laccase from the white rot fungus Trametes trogii. Appl. Microbiol. Biotechnol. 49: 545-551   DOI
33 Kweon, M. H., H. Jang, W. J. Lim, H. I. Chang, C. W. Kim, H. C. Yang, H. J. Hwang, and H. C. Sung. 1999. Anticomplementary properties of polysaccharides isolated from fruit bodies of mushroom Pleurotus ostreatus. J. Microbiol. Biotechnol. 9: 450-456