• Preventive Nutrition and Food Science
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• v.7 no.3
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• pp.317-319
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• 2002

Exo-polygalacturonase (EPG) from Rhizopus sp. was applied to the extraction of alginic acid from sea mustard to increase extraction yield. EPG digestion was examined under distinct conditions within temperatures from $25^{\circ}C$ to 5$0^{\circ}C$, pH 5 to 9, and treatment times from 0 to 36 hr. The optimal conditions fur alginic acid extraction with EPG were: pH 7.0 at 3$0^{\circ}C$ for 24 hrs. The EPG hot water extraction yield was 3.4 times higher yield than hot water extraction alone. Using EPG to extract alginic acid from sea mustard should be considered a viable alternative to conventional extraction, with the advantage of reducing hazardous wastes such as strong acid and alkali solutions.

• Journal of the Korean Society of Clothing and Textiles
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• v.27 no.11
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• pp.1350-1357
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• 2003

This research was aimed to establish the standard extraction and analytical procedures for examining the chromophoric substance in madder root with the ultimate goal of identifying the dyes in badly faded textiles of archaeological origin. The separation temperature of gas chromatography, pH and other extraction conditions were tested. The results were as follows: The suitable separation temperature for the GC cappillary column was 50∼305$^{\circ}C$, and methanol was a good GC solvent for both standard alizarin and madder extraction. The best extraction of madder was achieved by 90 min soaking in room temperature followed by filtration and the actual heat extraction procedure. The best pH for extracting alizarin was pH 3 and above pH 5 alizarin was not detectible. Only alizarin and no purpurin was found in the extraction of the currently used madder plant.

• Journal of Applied Biological Chemistry
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• v.57 no.3
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• pp.197-203
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• 2014

Various parameters such as solvent selection, concentration, solid/liquid ratio, soaking time, temperature, stationary, shaking conditions, and repeated extractions were investigated in order to determine the optimum extraction conditions of ${\beta}$-glucosidase from bagasse fermented by mixed culture of Aspergillus niger NRC 7A and Aspergillus oryzae NRRL 447. Among various solvents tested, non ionic detergents gave the best results than the inorganic or organic salt solutions and distilled water. The optimum conditions for extraction of ${\beta}$-glucosidase were 30 min soaking time at $40^{\circ}C$ under shaking condition at 150 rpm, with solid/liquid ratio 1:15 (w/v), which yielded $2882.74{\pm}95.52U/g$ fermented culture (g fc) of enzyme activity. With repeated washes under the above optimum conditions, the results showed that enzyme extracted in the $1^{st}$ and $2^{nd}$ washes represents about 90% of the total activity.

• Food Science and Preservation
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• v.8 no.2
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• pp.157-163
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• 2001

Optimal conditions for hot water extraction of Liriope spicata tuber were investigated with changes in solvent ratio(2∼6 fold) and heating time(1∼5 hr) by response surface methodology. The content of extractable solids increased with an increased in solvent ratio, and the highest content showed at heating time of 3 hr. The content of total steroid saponin increased with a decrease in solvent ratio, and increased with an increase in heating time at increasing the solvent ratio. The content of non-reducing sugar containing oligosaccharides at a lower solvent ratio didn’t show changes depending on heating time, while that at a higher ration decreased with an increase in heating time. Optimal extraction conditions using hot water as the limited conditions of 15∼18% extractable solids, 1.5∼2.0% total steroid saponin, 6∼8% reducing sugar, 6∼7% non-reducing sugar and 13∼15 brix were 3 hrs of heating time and 4 fold of solvent ratio.

• Journal of the Korean Home Economics Association
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• v.39 no.4
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• pp.49-59
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• 2001

Natural red and yellow dyestuff was extracted from safflower (Carthamus tinctorius Linnaeus) by a new process of cellulase extraction compared with the conventional hot water extraction. Dyestuffs were extracted from safflower easily and repeatedly by means of cellulose as safflower cell wall destroyer. It means that new dyestuff extraction by cellulase improves not only yields of dyestuff from safflower successfully but also the rate of repetition of extraction. From the above experiments, the conclusions of this study were summarized as follows. 1. The optimum conditions of dyestuff extraction from safflower by general extraction method were that the solvent was the water of pH 6.0 on yellow dyestuff and 3% $K_2CO_3$ solution on red dyestuff, extraction temperature was $55^{\circ}C$, and extraction time was 30 min. 2. Among various cellulase, the NOVO cellulase was the best cell wall destroyer of safflower and finally produced the largest amount of dyestuff from safflower by cellulase extraction method. 3. The optimum conditions of dyestuff extraction by cellulase extraction method were conducted on 10 unit of cellulase per gram of safflower at $100m{\ell}$ water of pH 5.0 at $50^{\circ}C$ for 30 min.

• Korean Chemical Engineering Research
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• v.50 no.3
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• pp.545-550
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• 2012

A 17-run Box-Behnken design (BBD) was used to optimize the extraction conditions of astaxanthin from shrimp waste. Three factors such as ratio of ethanol to raw material, extraction temperature ($^{\circ}C$) and extraction time (min) were investigated. The adjusted coefficient of determination ($R^2{_{adj}}$) for the model was 0.9218, and the probability value (p=0.0003) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized ratio of ethanol to raw material 29.7, extraction temperature $49.5^{\circ}C$ and extraction time 59.9 min. Under these conditions, the mean extraction yield of astaxanthin was $17.80{\mu}g/g$, which was in good agreement with the predicted model value. Under these conditions, validation experiments were done and the mean extraction yield of astaxanthin was $17.77{\mu}g/g$, which is in good agreement with the predicted model value.

• Korean Journal of Food Science and Technology
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• v.40 no.5
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• pp.540-544
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• 2008

In this study, response surface methodology was employed in order to optimize the limonin and nomilin extraction conditions from citron seed. The independent variables were extraction temperature, extraction time, and shaking velocity. The yield of limonin and nomilin increased with increased extraction temperature and time. The extraction effects on the limonin and nomilin were higher at extraction temperature (p<0.01). The predicted extraction conditions were validated through actual experiments. The predicted conditions were as follows: $49.7^{\circ}C$ of extraction temperature, 3.3 hr of extraction time, and 400.6 rpm of shaking velocity for the maximum limonin contents (353.9 mg/100 g) and $50.3^{\circ}C$, 3.5 hr, and 399.9 rpm for the maximum nomilin contents (214.5 mg/100 g). The experimental values of limonin and nomilin contents at the predicted conditions were 338.8 and 219.5 mg/100 g, respectively. The predicted values at the optimized conditions were acceptable when compared to the experimental values.

• Journal of the Korean Wood Science and Technology
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• v.48 no.6
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• pp.861-868
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• 2020

The use of polyphenol extracts from radiata pine (Pinus radiata) bark as raw materials for bio-foams was investigated along with the optimal NaOH extraction conditions. The targeted yield of alkaline extracts was 60%, and the targeted pH was 11 to 12. The radiata pine bark was composed of 70% of a 1% NaOH extract, which contained mainly polyphenols, such as proanthocyanidin (PA). As the particle size of the bark decreased, the yield of the 1% NaOH extracts increased from 57 to 87%. A range of NaOH concentrations, liquor ratios, and extraction times were explored to establish an economic polyphenol extraction method. More than 60% of the alkaline extract was extracted, and the pH of the extract was approximately 12 when the optimum extraction conditions were employed, i.e., a liquor to bark ratio of 5:1, a NaOH dosage of 17 to 18% based on the bark weight, and a 1 h extraction time. Following neutralization of the alkaline extract, structural analysis indicated severe structural changes in the PA during the alkaline extraction. Because the alkaline extract was barely soluble in the solvent used for the structural analyses, it is assumed that chemical modification is required to increase the solubility of the alkaline extract for the production of bio-foams.

• Food Science and Biotechnology
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• v.16 no.2
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• pp.299-305
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• 2007

Response surface methodology was employed to optimize extraction conditions for finding the maximal functional properties of Pleurotus eryngii. Based on central composite design, the study plan was established with variations of microwave power (30-150 W), ethanol concentration (0-99.9%), and extraction time (1-9 min). Regression analysis was applied to obtain a mathematical model. A maximal yield of 47.86% was obtained when the microwave power, ethanol concentration, and extraction time were set at 122.7 W, 42.14%, and 8.3 min, respectively. A maximized electron donating ability of 93.32% was found under the following conditions: a microwave power of 144.19 W, an ethanol concentration of 49.52%, and an extraction time of 6.7 min. When the microwave power, ethanol concentration, and extraction time were set at 125.43 W, 40.54%, and 8.1 min, respectively, the maximum nitrite-scavenging ability was 80.47%. The optimum ranges of the extraction conditions, superimposed by the response surface methodology, could predicate a microwave power of 110-150 W, ethanol concentration of 0-45%, and extraction time of 7-9 min.

• Membrane and Water Treatment
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• v.6 no.4
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• pp.277-292
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• 2015

The extraction of lactic acid by an emulsion liquid membrane (ELM), in batch and continuous mode, has been reported. On the basis of preliminary experiments, the optimum composition of the organic phase (membrane) is determined. When the SPan 80 is used as surfactant, the emulsion breakage exceeds 50%, but only 10% is obtained when the ECA4360 is used. The effects of surfactant, carrier and solute concentrations, phase volume ratio, and stirring speed on the extraction yield were examined and optimized. Surfactant, carrier and diluent used were ECA4360, trilaurylamine (TLA) and dodecane, respectively; 2-ethylhexane-1,3-diol (EHD) is used as a co-surfactant. Under optimal conditions, emulsion breaking is very low and the swelling is kept at its lowest level. Under the pH conditions of fermentation medium, the extraction yield is lower. A mixer-settler continuous system was used for testing these conditions. The residence time, the number of extraction stages and the stability of the emulsion were studied and optimized. The extraction yield obtained exceeds 90%.