Browse > Article
http://dx.doi.org/10.3746/jfn.2002.7.3.317

Use of Exo-polygalacturonase to Improve Extraction Yields of Alginic Acid from Sea Mustard (Undaria pinnatifida)  

Lee, Seung-Cheol (Department of Food Science and Biotechnology, Kyungnam University)
Oh, Jeong-Hoon (Department of Food Science and Biotechnology, Kyungnam University)
Hwang, Yong-Il (Department of Food Science and Biotechnology, Kyungnam University)
Kim, Jeong-Mok (Department of Food Science and Biotechnology, Mokpo National University)
Publication Information
Preventive Nutrition and Food Science / v.7, no.3, 2002 , pp. 317-319 More about this Journal
Abstract
Exo-polygalacturonase (EPG) from Rhizopus sp. was applied to the extraction of alginic acid from sea mustard to increase extraction yield. EPG digestion was examined under distinct conditions within temperatures from $25^{\circ}C$ to 5$0^{\circ}C$, pH 5 to 9, and treatment times from 0 to 36 hr. The optimal conditions fur alginic acid extraction with EPG were: pH 7.0 at 3$0^{\circ}C$ for 24 hrs. The EPG hot water extraction yield was 3.4 times higher yield than hot water extraction alone. Using EPG to extract alginic acid from sea mustard should be considered a viable alternative to conventional extraction, with the advantage of reducing hazardous wastes such as strong acid and alkali solutions.
Keywords
alginic acid extraction; exo-polygalacturonase; sea mustard;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Sakai T, Yoshitaka S. 1984. Purification and some properties of a protopectin solubilizing enzyme from Galactomyces reessii strain L. Agric Biol Chem 48: 1941-1950   DOI
2 Haug A, Larsen B. 1965. A study of the constitution of alginic acid by partial acid hydrolysis. Paper presented at the 5th Proc. Int. Seaweed Sym. Pergamon Press, Oxford. p 271- 277
3 Nishide E, Kinoshita Y, Anzai H, Uchida N. 1988. Distribution of hot-water extractable material, water-soluble alginate and alkali-soluble alginate in different parts of Undaria pinnatifida. Nippon Suisan Gakkaishi 54: 1619-1622   DOI
4 Sakai T, Okushima M. 1982. Purification and crystallization of a protopectin solubilizing enzyme from Trichosporon penicillatum. Agric Biol Chem 46: 667-676   DOI
5 Lee SC, Ko BS, Lee DH, Hwang YI. 1997. Cell separation of vegetable tissues by protopectinase. J Korean Soc Food Sci Nutr 26: 430-435
6 Lee SC, Ko BS, Kim HM, Kim KW, Hwang YI. 1997. Isolation of Rhizopus sp. R2 producing protopectinase and optimum condition for preparing single cells from potato tissues. Korean J Microbiol 33: 131-135
7 Sakai T, Sakamoto T, Hallaert J, Vandamme EJ. 1993. Pectin, pectinase and protopectinase: production, properties, and applications. Adv Appl Microbiol 39: 213-294   DOI
8 Sakai T, Hours R, Nakamura T. 1995. Enzymatic maceration of vegetables with protopectinases. J Food Sci 60: 468-472   DOI   ScienceOn
9 Mitsui T, Hashimoto N, Deguchi K, Hirano M, Igaue L. 1990. Isolation of plant mesophyll protoplasts with and endo- polygalacturonase from Trichosporon penicillatum. Plant Tissue Cult Lett 7: 14-18   DOI
10 Blumenkrantz N, Asboe-Hansen G. 1973. New method for quantitative determination of uronic acids. Anal Biochem 54: 484-489   DOI   ScienceOn
11 Suzuki H, Abe T, Urade M, Nishizawa K, Kuroda A. 1967. Nature of the macerating enzymes from Rhizopus sp. J Ferment Technol 45: 73-85
12 Lee SC, Yuk HG, Bae SM, Hwang YI, Choi JS, Cho YJ. 1999. Extraction of pectin with exo-polygalacturonase from apple pomace. Korean J Food Sci Technol 31: 68-73
13 Yuk HG, Choi JH, Cho YJ, Ha JU, Hwang YI, Lee SC. 1999. Investigation of reactive conditions to extract pectin with exo-polygalacturonase from pear pomace. Korean J Food Sci Technol 31: 971-976
14 Sakai T, Okushima M, Yoshitaka S. 1984. Purification, crystallization and some properties of endopolygalacturonase from Kluyveromyces fragilis. Agric Biol Chem 48: 1951-1961   DOI