• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.627-632
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• 2007

FoxO1, FoxO3a and FoxO4 belong to the FoxO gene family, which play important roles in the PI3K/PKB pathway. In this study, we cloned the porcine FoxO1, FoxO3a and FoxO4 sequences and assigned them to SSC11p11-15, SSC1p13 and SSC xq13 using somatic cell hybrid panel (SCHP) and radiation hybrid panel (IMpRH). RT-PCR results showed that these three genes are expressed in multiple tissues. Sequencing of PCR products from different breeds identified a synonymous T/C polymorphism in exon 2 of FoxO3a. This FoxO3a single nucleotide polymorphism (SNP) can be detected by AvaII restriction enzyme. The allele frequencies of this SNP were investigated in Dahuabai, Meishan, Tongcheng, Yushan, Large White, and Duroc pigs. Association of the genotypes with growth and carcass traits showed that different genotypes of FoxO3a were associated with carcass length and backfat thickness between 6th and 7th ribs (BTR) and drip loss (p<0.05).

• Journal of Embryo Transfer
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• v.19 no.3
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• pp.291-299
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• 2004

Characterization of quantitative trait loci (QTL) was investigated in the experimental cross population between Berkshire and Yorkshire breed. A total of 512 F$_2$ offspring from 65 matting of F$_1$ parents were phenotyped the carcass traits included average daily gain (ADG), average backfat thickness (ABF), tenth rip backfat thickness (TRF), loin eye area (LEA), and last rip backfat thickness (LRF). All animals were genotyped for 125 markers across the genome. Marker linkage maps were derived and used in QTL analysis based on line cross least squares regression interval mapping. A decision tree to identify QTL with imprinting effects was developed based on tests against the Mendelian mode of QTL expression. To set the evidence of QTL presence, empirical significance thresholds were derived at chromosome-wise and genome-wise levels using specialized permutation strategies. Significance thresholds derived by the permutation test were validated in the data set based on simulation of a pedigree and data structure similar to the Berkshire-Yorkshire population. Genome scan revealed significant evidences for 13 imprinted QTLs affecting growth and body compositions of which nine were identified to be QTL with paternally expressed inheritance mode. Four of QTLs in the loin eye area (LEA), and tenth rip backfat thickness (TRF), a maternally expressed QTL were found on chromosome 10 and 12. These results support the useful statistical models to analyse the imprinting far the QTLs related carcass trait.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.5
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• pp.413-420
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• 2012

In this study, the Kernel integration scheme for 2D linear elastic direct boundary element method has been discussed on the basis of subparametric element. Usually, the isoparametric based boundary element uses same polynomial order in the both basis function and mapping function. On the other hand, the order of mapping function is lower than the order of basis function to define displacement field when the subparametric concept is used. While the logarithmic numerical integration is generally used to calculate Kernel integration as well as Cauchy principal value approach, new formulation has been derived to improve the accuracy of numerical solution by algebraic modification. The subparametric based direct boundary element has been applied to 2D elliptical partial differential equation, especially for plane stress/strain problems, to demonstrate whether the proposed algebraic expression for integration of singular Kernel function is robust and accurate. The problems including cantilever beam and square plate with a cutout have been tested since those are typical examples of simple connected and multi connected region cases. It is noted that the number of DOFs has been drastically reduced to keep same degree of accuracy in comparison with the conventional isoparametric based BEM. It is expected that the subparametric based BEM associated with singular Kernel function integration scheme may be extended to not only subparametric high order boundary element but also subparametric high order dual boundary element.

• Journal of the korean academy of Pediatric Dentistry
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• v.33 no.2
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• pp.304-310
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• 2006

Prevotella intermedia is one of the most frequently implicated pathogens in human periodontal disease and has a requirement for hemin for growth. This study has identified a hemin-binding P. intermedia protein by expression of a P. intermedia genomic library in Escherichia coli, a bacterium which does not require or transport exogenous hemin. The genomic library of P. intermedia was constructed into plasmid pUC18, transformed into Escherichia coli strain $DH5{\alpha}$, and screened for recombinant clones using heminbinding activity by plating onto hemin-containing agar. Approximately 5,000 recombinant E. coli colonies were screened onto LB-amp-hemin agar, single clone(pHem1) was exhibited a clearly pigmented phonotype. The 2.5kb insert DNA of pHem1 was determined by restriction enzyme mapping. Southern blot analysis of BamHI, BglII, EcoRI, HindIII and PstI-digested P. intermedia DNA indicated that single copy of the gene was present in the genome. Northern blot analysis revealed that the size of transcript was approximately 1.8 kb. The cloned gene contained a single ORF, consisting of approximately 850-residue amino acids. A BLAST search of the Institute for Genomic Research genes with similar nucleotide sequence revealed no significant similarity It needs further investigation to clarify the mechanisms of heme uptake in P. intermedia.

• Journal of the Korean association of regional geographers
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• v.17 no.1
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• pp.40-57
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• 2011

A feng-shui map is a kind of map that features such propitious positions as fortune-bringing spots or ideal grave sites. The map is a representation of the most ideal natural terrains in terms of feng-shui perspectives. This study did research on two feng-shui maps registered on some genealogical tables in order to see how the map was drawn up. In addition, the detailed characteristics of landform expressions like the frame of the map, viewpoints, center-oriented mapping, water flow, mountain theories were delved into. The results of the research are as follows: the feng-shui maps for this research used the techniques of double scale and aerial view, described terrains with grave sites in their centers, and enlarged important terrains compared with surroundings for exaggeration purposes. In addition, other vital landforms not observed from grave sites were depicted with viewpoints moved and were represented in fine details. The part of mountain theories had the following points as their main subject matter: geographical locations, overall orientations of mountain ranges, topographical traits, grave seat directions and surrounding terrains.

• Korean Journal of Cognitive Science
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• v.12 no.1_2
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• pp.77-89
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• 2001

Applying perceptual hierarchy of facial feature points, a neural network model for recognizing facial expressions was designed. Input data were convolution values of 150 facial expression pictures by Gabor-filters of 5 different sizes and 8 different orientations for each of 39 mesh points defined by MPEG-4 SNHC (Synthetic/Natural Hybrid Coding). A set of multiple regression analyses was performed with the rating value of the affective states for each facial expression and the Gabor-filtered values of 39 feature points. The results show that the pleasure-displeasure dimension of affective states is mainly related to the feature points around the mouth and the eyebrows, while a arousal-sleep dimension is closely related to the feature points around eyes. For the filter sizes. the affective states were found to be mostly related to the low spatial frequency. and for the filter orientations. the oblique orientations. An optimized neural network model was designed on the basis of these results by reducing original 1560(39x5x8) input elements to 400(25x2x8) The optimized model could predict human affective rating values. up to the correlation value of 0.886 for the pleasure-displeasure, and 0.631 for the arousal-sleep. Mapping the results of the optimized model to the six basic emotional categories (happy, sad, fear, angry, surprised, disgusted) fit 74% of human responses. Results of this study imply that, using human principles of recognizing facial expressions, a system for recognizing facial expressions can be optimized even with a a relatively little amount of information.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.140-140
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• 2017

Nutritious and functional foods from crop have received great attention in recent years. Colored-grain wheat contains high phenolic compound and a large number of flavonoid. The anthocyanin and polyphenolic synthesis and accumulation is generally stimulated in response to biotic or abiotic stresses. Here, we analyzed genome wide transcripts in seedling of colored-grain wheat response to ABA and PEG treatment. About 900 and 1500 transcripts (p-value < 0.05) from ABA and PEG treatment were aligned to IWGSC1+popseq DB which is composed of over 110,000 transcripts including 100,934 coding genes. NR protein sequences of Poaceae from NCBI and protein sequence of transcription factors originated from 83 species in plant transcription factor database v3.0 were used for annotation of putative transcripts. Gene ontology analysis were conducted and KEGG mapping was performed to show expression pattern of biosynthesis genes related in flavonoid, isoflavonoid, flavons and anthocyanin biopathway. DroughtDB (http://pgsb.helmholtz-muenchen.de/droughtdb/) was used for detection of DEGs to explain that physiological and molecular drought avoidance by drought tolerance mechanisms. Drought response pathway, such as ABA signaling, water and ion channels, detoxification signaling, enzymes of osmolyte biosynthesis, phospholipid metabolism, signal transduction, and transcription factors related DEGs were selected to explain response mechanism under water deficit condition. Anthocyanin, phenol compound, and DPPH radical scavenging activity were measured and antioxidant activity enzyme assays were conducted to show biochemical adaptation under water deficit condition. Several MYB and bHLH transcription factors were up-regulated in both ABA and PEG treated condition, which means highly expressed MYB and bHLH transcription factors enhanced the expression of genes related in the biosynthesis pathways of flavonoids, such as anthocyanin and dihydroflavonols in colored wheat seedlings. Subsequently, the accumulation of total anthocyanin and phenol contents were observed in colored wheat seedlings, and antioxidant capacity was promoted by upregulation of genes involved in maintaining redox state and activation of antioxidant scavengers, such as CAT, APX, POD, and SOD in colored wheat seedlings under water deficit condition. This work may provide valuable and basic information for further investigation of the molecular responses of colored-grain wheat to water deficit stress and for further gene-based studies.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.33 no.1
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• pp.25-45
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• 2022

This study was to develop KORMARC-bibliographic format reflecting cataloging trends after LRM using literature review, analysis of MARC 21 discussion papers, and comparison of the fields in MARC 21 and KORMARC. The acceptance and consideration of fields and sub-fields that need to be revised in KORMARC are as follows. First, in terms of LRM / RDA, fields 381 or 387 for the representative expression, field 881 and the change and addition of its sub-fields for the manifestation statement, and data provenance code to ▾7 sub-field for date provenance may be considered. Second, in terms of Linked Data, ▾1 sub-field for RWO, and field 758 for related work identifier can be added. Third, for the data exchange of KORMARC and BIBFRAME, it should be developed in consideration of mapping with BIBFRAME classes and attributes in KORMARC. Fourth, additional fields such as 251 version information, 334 mode of issuance, 335 expansion plan, 341 accessibility content, 348 format of notated music, 353 supplementary content characteristics, 532 accessibility note, 370 associated place, 385 audience characteristics, 386 creator/contributor characteristics, 388 time period of creation, 688 subject added entry-type of entity unspecified, 884 description conversion information, 885 matching information could be developed. This study will be used to revise KORMARC-bibliographic format and to build and utilize bibliographic data in domestic libraries.

• Journal of KIISE:Databases
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• v.30 no.6
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• pp.641-650
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• 2003

The eXtensible Markup Language(XML) is emerging as a standard format of data representation and exchange on the Internet. There have been researches about storing and retrieving XML documents using the relational database which has techniques in full growth about large data processing, recovery, concurrency control and so on. Since in previous systems same structure information and fundamental operation are used for processing of various kinds of XML queries, only some specific query can be efficiently processed not all types of query. In this paper, we propose a flexible query processing system. To process query efficiently, the proposed system analyzes regular path expression queries, and uses $\theta$-join operation using region numbering values to check ancestor-descendent relationship and equi-join operation using parent's region start value to check parent-child relationship. Thus, the proposed system processes efficiently XML regular path expressions. From the experimental results, we show that proposed XML query processing system is more efficient than previous systems.

• Journal of Microbiology and Biotechnology
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• v.6 no.5
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• pp.309-314
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• 1996

Alcaligenes sp. SH-69 can synthesize poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from a single carbon source such as glucose. To clone the phaC gene from Alcaligenes sp. SH-69, a polymerase chain reaction was performed using the oligomers synthesized based on the conserved regions of the phaC genes from other bacteria. A PCR product (550 bp) was partially sequenced and the deduced amino acid sequence was found to be homologous to that of the phaC gene from Alcaligenes eutrophus. Using the PCR fragment Southern blotting of Alcaligenes sp. SH-69 genomic DNA digested with several restriction enzymes was carried out. To prepare a partial genomic library, about 5-Kb genomic DNA fragments digested with EcoRI, which showed a positive signal in the Southern blotting, were eluted from an agarose gel, ligated with pUC19 cleaved with EcoRI, and transformed into Escherichia coli. The partial library was screened using the PCR fragment as a probe and a plasmid, named pPHA11, showing a strong hybridization signal was selected. Restriction mapping of the insert DNA in pPHA11 was performed. Cotransformation into E. coli of the plasmid pPHA11 and the plasmid pPHA21 which has phaA and phaB from A. eutrophus resulted in turbid E. coli colonies which are indicative of PHA accumulation. This result tells us that the Alcaligenes sp. SH-69 phaC gene in the pPHA11 is functionally active in E. coli and can synthesize PHA in the presence of the A. eutrophus phaA and phaB genes.