• ETRI Journal
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• 제43권5호
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• pp.869-880
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• 2021

Herein, we estimate the direction of arrival (DOA) of non-Gaussian signals for nested arrays (NAs) by implementing the fourth-order difference co-array (FODC) and successive methods. In particular, considering the property of the fourth-order cumulant (FOC), we first construct the FODC of the NA, which can obtain O(N⁴) virtual elements using N physical sensors, whereas conventional FOC methods can only obtain O(N²) virtual elements. In addition, the closed-form expression of FODC is presented to verify the enhanced degrees of freedom (DOFs). Subsequently, we exploit the vectorized FOC (VFOC) matrix to match the FODC of the NA. Notably, the VFOC matrix is a single snapshot vector, and the initial DOA estimates can be obtained via the discrete Fourier transform method under the underdetermined correlation matrix condition, which utilizes the complete DOFs of the FODC. Finally, fine estimates are obtained through the spatial smoothing-Capon method with partial spectrum searching. Numerical simulation verifies the effectiveness and superiority of the proposed method.

• Journal of Animal Science and Technology
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• 제61권2호
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• pp.61-68
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• 2019

The hG-CSF (human Granulocyte Colony-Stimulating Factor) is a growth and stimulation factor capable of inducing the proliferation of bone marrow cells, several types of leukocytes, among other hematopoietic tissue cells. hG-CSF is used in used to treat anomalies that reder a small number of circulating white blood cells, which may compromise the immune defenses of the affected person. For these reasons, the production of hG-CSF in a bioreactor system using the mammary gland of genetic modified animals is a possibility of adding value to the bovine genetic material and reducing the costs of hG-CSF production in pharmaceutical industry. In this study, we aimed the production of transgenic hG-CSF bovine through the lipofection of bovine primary fibroblasts with an hG-CSF expression cassette and cloning these fibroblasts by the somatic cell nuclear transfer (SCNT) technique. The bovine fibroblasts transfected with the hG-CSF cassette presented a stable insertion of this construct into their genome and were efficiently synchronized to G0/G1 cell cycle stage. The transgenic fibroblasts were cloned by SCNT and produced 103 transferred embryos and 2 pregnancies, one of which reached 7 months of gestation.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2019년도 춘계학술대회
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• pp.479-481
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• 2019

최근, 가상현실(VR) 기술을 이용하여 다양한 산업 분야의 훈련 시스템을 제작하여 교육에 널리 활용하고 있다. 가상현실 기반 훈련 시스템은 안전할 뿐만 아니라 재료의 낭비도 없고, 언제 어디서든 실습을 해 볼 수가 있어서 여러 가지 장점이 있다. 예를 들면, 가상현실 용접 훈련 시뮬레이션 시스템은 몰입형 환경에서 철판 모재의 접합을 실제와 같이 수행할 수 있어서 현장 작업자 및 신입 교육 훈련에 널리 쓰이고 있다. 이 때 철판 모재 접합을 사실적으로 표현하는 것이 훈련의 효과를 극대화하기 위해 중요하지만 기존의 기술로는 접합의 효과를 자연스럽게 표현하기에는 한계가 있었다. 본 연구에서는 용접 훈련 시스템을 구축하기 위해 접합 효과를 쉐이더 기반으로 표현하는 방법을 경험 DB와 수학적 모델 기반으로 제시하고자 한다. 본 연구 논문의 결과를 용접 훈련 시스템에 적용하면 철판 모재의 접합 가시화를 사용자에게 정밀도 높게 제공하여 용접 훈련 효과를 보다 향상하는데 도움을 줄 수 있겠다.

• 영미문화
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• 제14권1호
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• pp.73-94
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• 2014

Accounts from therapeutic culture seem often to associate the selfish, or at least self-centered quest for self-fulfillment with individual choice or satisfaction, self-expression, expressive individualism, and emotionalism. These associations point to the downside of therapy as they present it as constituting a culture of narcissism, selfishness, or irresponsibility. While some of these characterizations contain useful insights, they overlook what are maybe some of the most important features of a therapeutic outlook. This paper aims to reveal that the therapeutic imperative is not so much geared towards the realization of self-fulfillment, as it is the promotion of self-limitation. Therapeutic culture tends to posit the self in a fragile and feeble form and insist that the management of life requires the continuous intervention of therapeutic expertise. Because of this, the elevated concern with the self is underpinned by anxiety, pain, suffering, and survival, rather than seen as a positive vision of realizing the human potential. Therapeutic culture has in this way helped to construct a diminished sense of self by which one is seen as suffering from an emotional deficit and vulnerability. This paper demonstrates this downside of therapeutic culture concerning self-limitation and the sense of a diminished self by examining popular "misery memoirs." Misery memoirs are widely consumed by the general public, therefore tend to be treated by contemporary therapeutic culture as a gospel on the therapeutic ideal for self-fulfillment and self-discovery. This is, despite the existence of hidden evidence to the contrary, because of their redemptive, happy endings that show individuals overcoming difficult trials such as child abuse, incestuous rape, and domestic violence. Individual self-fulfillment and self-discovery in such stories are not achieved through the active agency of the subject but through the passive endurance of pathological symptoms and with the aid of expertise and outside support. Therefore, such stories put victims in the limited position.

• 한국미생물·생명공학회지
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• 제49권4호
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• pp.510-518
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• 2021

Bacteriophages are considered excellent sensing elements for platforms detecting bacteria. However, their lytic cycle has restricted their efficacy. Here, we used the minor coat protein pIII domain (N1N2) of phage CTXφ to construct a novel surface plasmon resonance (SPR) biosensor that could detect Vibrio cholerae. N1N2 harboring the domains required for phage adsorption and entry was obtained from Escherichia coli using recombinant protein expression and purification. SDS-PAGE revealed an approximate size of 30 kDa for N1N2. Dot blot and transmission electron microscopy analyses revealed that the protein bound to the host V. cholerae but not to non-host E. coli K-12 cells. Next, we used amine-coupling to develop a novel recombinant N1N2 (rN1N2)-functionalized SPR biosensor by immobilizing rN1N2 proteins on gold substrates and using SPR to monitor the binding kinetics of the proteins with target bacteria. We observed rapid detection of V. cholerae in the range of approximately 10³ to 10⁹ CFU/ml but not of E. coli at any tested concentration, thereby confirming that the biosensor exhibited differential recognition and binding. The results indicate that the novel biosensor can rapidly monitor a target pathogenic microorganism in the environment and is very useful for monitoring food safety and facilitating early disease prevention.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.740-748
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• 2022

As circ_UBE2D2 has been confirmed to have targeted binding sites with multiple miRNAs involved in septic acute kidney injury (SAKI), efforts in this study are directed to unveiling the specific role and relevant mechanism of circ_UBE2D2 in SAKI. HK-2 cells were treated with lipopolysaccharide (LPS) to construct SAKI model in vitro. After sh-circ_UBE2D2 was transfected into cells, the transfection efficiency was detected by qRT-PCR, cell viability and apoptosis were determined by MTT assay and flow cytometry, and expressions of Bcl-2, Bax and Cleaved-caspase 3 were quantified by western blot. Target genes associated with circ_UBE2D2 were predicted using bioinformatics analysis. After the establishment of SAKI rat model, HE staining and TUNEL staining were exploited to observe the effect of circ_UBE2D2 on tissue damage and cell apoptosis. The expression of circ_UBE2D2 was overtly elevated in LPS-induced HK-2 cells. Sh-circ_UBE2D2 can offset the inhibition of cell viability and the promotion of cell apoptosis induced by LPS. Circ_UBE2D2 and miR-370-3p as well as miR-370-3p and NR4A3 have targeted binding sites. MiR-370-3p inhibitor reversed the promoting effect of circ_UB2D2 silencing on viability of LPS-treated cells, but shNR4A3 neutralized the above inhibitory effect of miR-370-3p inhibitor. MiR-370-3p inhibitor weakened the down-regulation of NR4A3, Bax and Cleaved caspase-3 and the up-regulation of Bcl-2 induced by circ_UB2D2 silencing, but these trends were reversed by shNR4A3. In addition, sh-circ_UBE2D2 could alleviate the damage of rat kidney tissue. Circ_UBE2D2 mitigates the progression of SAKI in rats by targeting miR-370-3p/NR4A3 axis.

• Journal of Ginseng Research
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• 제47권2호
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• pp.228-236
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• 2023

Background: QiShen YiQi pills (QSYQ) is a Traditional Chinese Medicine (TCM) formula, which has a significant effect on the treatment of patients with myocardial infarction (MI) in clinical practice. However, the molecular mechanism of QSYQ regulation pyroptosis after MI is still not fully known. Hence, this study was designed to reveal the mechanism of the active ingredient in QSYQ. Methods: Integrated approach of network pharmacology and molecular docking, were conducted to screen active components and corresponding common target genes of QSYQ in intervening pyroptosis after MI. Subsequently, STRING and Cytoscape were applied to construct a PPI network, and obtain candidate active compounds. Molecular docking was performed to verify the binding ability of candidate components to pyroptosis proteins and oxygen-glucose deprivation (OGD) induced cardiomyocytes injuries were applied to explore the protective effect and mechanism of the candidate drug. Results: Two drug-likeness compounds were preliminarily selected, and the binding capacity between Ginsenoside Rh2 (Rh2) and key target High Mobility Group Box 1 (HMGB1)was validated in the form of hydrogen bonding. 2 μM Rh2 prevented OGD-induced H9c2 death and reduced IL-18 and IL-1β levels, possibly by decreasing the activation of the NLRP3 inflammasome, inhibiting the expression of p12-caspase1, and attenuating the level of pyroptosis executive protein GSDMD-N. Conclusions: We propose that Rh2 of QSYQ can protect myocardial cells partially by ameliorating pyroptosis, which seems to have a new insight regarding the therapeutic potential for MI.

• 사회복지연구
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• 제42권3호
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• pp.121-155
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• 2011

본 연구는 교도소와 치료감호소에 수감되어 있는 성폭력 가해자의 경험에 대한 연구로서 이론적으로는 성폭력 가해자의 경험의 본질구조를 살펴보고자 했고 실천적으로는 성폭력 가해자들의 재범을 막고 치료적 감호프로그램을 제공하기 위한 사회복지실천 개입의 근거 자료를 구축하고자 했다. 연구는 Van-Manen(1990)의 분석틀을 원용하여 해석학적 현상학적 방법으로 수행했고 연구에는 7명의 성폭력 가해자가 참여했다. 자료는 심층면담으로 수집했으며 해석한 결과는 다음과 같다. 여론의 피해자라는 인식, 카인의 표식, 견본생활(show window)로 인한 성찰기회의 상실, 구별짓기(distinction)를 통한 집단차별화, 허물을 벗고자 하는 형이상학적 존재의 발현, 과학적 관리기술의 역이용, 상상계(the Imaginary)로부터의 미분화(undifferentiation), 도치된 소통으로 나타난 성행위, 통제받음의 반동형성으로서 지배영역을 찾음, 아동의 성마저 상품화하는 자본주의 속성에서 자유로울 수 없음, 성정체성 사회화과정에서의 왜곡, 억눌린 욕망의 화신으로 각인된 부상, 상실된 어머니의 순결성 회복, 산산이 부서진 이름으로서의 가족 등으로 나타났다.

• 응용통계연구
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• 제37권3호
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• pp.265-282
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• 2024

기대 수익률은 높이고, 재무적 위험은 낮추는 것을 목표로 하는 금융 포트폴리오 최적화 분야에서는 위험 측정 지표에 관한 연구가 활발히 진행되어 왔다. 적은 자산으로 효율적인 포트폴리오를 구성하기 위해 다양한 페널티 항을 활용한 연구 또한 지속해서 진행되어 왔다. 본 논문에서는 평균-숏폴 포트폴리오와 SLOPE 페널티 항을 결합한 새로운 포트폴리오 최적화 식을 제안하였다. 이 과정에서 선형계획법으로 표현되지 않는 최적화식을 새로운 변수를 도입해 표현하고, 이를 ADMM 알고리즘을 사용해 해결하는 방식을 제안하였다. SLOPE 페널티 항이 갖는 그룹화 특징이 본 논문에서 제안하는 평균-숏폴 포트폴리오에서도 해당 특징이 유효함을 시뮬레이션 데이터를 바탕으로 확인하였다. 실증 데이터 분석을 통해서는 본 논문에서 제안한 모형을 기반으로 실제 투자 환경에서 고려할 수 있는 4가지 종류의 포트폴리오 구성 방식을 제안하고, 평가하였다.

• Wind and Structures
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• 제39권2호
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• pp.85-100
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• 2024

In light of extreme value distribution probability, an improved prediction method of the Recurrence Period Wind Speed (RPWS) is constructed considering wind direction, with the Equivalent Independent Wind Direction Number (EIWDN) introduced as a parameter variable. Firstly, taking the RPWS prediction of Beijing city as an example, the traditional Cook method is used to predict the RPWS of each wind direction based on the measured wind speed data in Beijing area. On basis of the results, the empirical formulae to determine the parameter variables are fitted to construct an improved expression of the non-exceedance probability of the RPWS. In this process, the statistical model of the optimal threshold is established, and thus the independent wind speed samples exceeding the threshold are extracted and fitted to follow the Generalized Pareto Distribution (GPD) model for analysis. In addition, the Extreme Value Type I (EVT I) distribution model is used to predict and analyze the RPWS. To verify its wide applicability, the improved method is further used in cities like Jinan, Nanjing, Wuxi, Shanghai and Shenzhen to predict and analyze the RPWS of each wind direction, and the prediction results are compared against those gained via the traditional Cook method and the whole direction. Results show that the 50-year RPWS results predicted by the improved method are basically consistent with those predicted by the traditional method, and the RPWS prediction values of most wind directions are within the envelope range of the whole wind direction prediction value. Compared with the traditional method, the improved method can readily predict the RPWS under different return periods through empirical formulae, and avoid the repeated operation process and some assumptions in the traditional Cook method, and then improve the efficiency of prediction. In addition, the improved RPWS prediction results corresponding to the GPD model are slightly larger than those of the EVT I distribution model.