• Clinical and Experimental Reproductive Medicine
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• 제43권1호
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• pp.9-14
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• 2016

Objective: Autophagy contributes to the clearance and recycling of macromolecules and organelles in response to stress. We previously reported that vitrified mouse oocytes show acute increases in autophagy during warming. Herein, we investigate the potential role of Atg7 in oocyte vitrification by using an oocyte-specific deletion model of the Atg7 gene, a crucial upstream gene in the autophagic pathway. Methods: Oocyte-specific Atg7 deficient mice were generated by crossing Atg7 floxed mice and Zp3-Cre transgenic mice. The oocytes were vitrified-warmed and then subjected to in vitro fertilization and development. The rates of survival, fertilization, and development were assessed in the Atg7 deficient oocytes in comparison with the wildtype oocytes. Light chain 3 (LC3) immunofluorescence staining was performed to determine whether this method effectively evaluates the autophagy status of oocytes. Results: The survival rate of vitrified-warmed $Atg7^{f/f}$;Zp3-Cre ($Atg7^{d/d}$) metaphase II (MII) oocytes was not significantly different from that of the wildtype ($Atg7^{f/f}$) oocytes. Fertilization and development in the $Atg7^{d/d}$ oocytes were significantly lower than the $Atg7^{f/f}$ oocytes, comparable to the $Atg5^{d/d}$ oocytes previously described. Notably, the developmental rate improved slightly in vitrified-warmed $Atg7^{d/d}$ MII oocytes when compared to fresh $Atg7^{d/d}$ oocytes. LC3 immunofluorescence staining showed that this method can be reliably used to assess autophagic activation in oocytes. Conclusion: We confirmed that the LC3-positive signal is nearly absent in $Atg7^{d/d}$ oocytes. While autophagy is induced during the warming process after vitrification of MII oocytes, the Atg7 gene is not essential for survival of vitrified-warmed oocytes. Thus, induction of autophagy during warming of vitrified MII oocytes seems to be a natural response to manage cold or other cellular stresses.

• Journal of Advanced Marine Engineering and Technology
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• 제33권2호
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• pp.267-271
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• 2009

The Marine Environment Protection Committee(MEPC) which is the IMO's specialized committee on marine pollution related matters deals with GHG related issues to discuss and compile possible approaches on technical, operational and market based measures to address GHG emissions from ships. The nitrous oxide($N_2O$) which remains generally in the atmosphere for around 114 years is one of the green house gases. The global warming potential of $N_2O$ is 310 times than $CO_2$ in the given period 100 years. It seems that the $N_2O$ formation is influenced by the nitrogen compound contained in the fuel which is named as "Fuel N" during the combustion process or the NOx, SOx and $H_2O$ through the emission gases before exhausted into the atmosphere. This paper has carried out an experimental study of the $N_2O$ concentration profiles by the change of $NH_3$ flows in the planner premixed combustion with using $C_3H_8$ and air.

• 대한간호학회지
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• 제36권2호
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• pp.403-414
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• 2006

Purpose: The purpose of this study was to investigate the effects of constraint-induced (CI) movement using self-efficacy on U/E function of chronic hemiplegic patients. CI movement discourages the use of the unaffected U/E, combined with intensive training of the affected U/E. Method: A non-equivalent pretest-posttest design was used. Study subjects were 40 hemiplegic patients conveniently selected from 2 different community health centers. The experimental subjects participated in the CI movement program for 6 hours daily over a period of two weeks. The exercises for affected U/E consisted of warming up, main exercise and ADL practice. To encourage the participants' behaviors self-efficacy enhancing strategies were used, which included performance accomplishment, vicarious experience, verbal persuasion and emotional arousal. Result: After 2 weeks of treatment, the grip power, pinch power, wrist flexion/extension, elbow flexion, and shoulder flexion/extension were significantly higher in the experimental subjects than in the control subjects. However, there was no significant difference in hand functions of the two groups. Conclusion: The above results show that the constraint-induced movement using self-efficacy could be an effective nursing intervention for improving U/E function of chronic hemiplegic patients. Long term studies are needed to determine the lasting effects of constraint-induced movement.

• 한국농공학회지
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• 제16권2호
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• pp.3395-3409
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• 1974

The purpose of this study is to test and compare the efficiency of heating-system for materials and construction of the wall, ceiling and window in soil brick house, cement house and boulder house respectively, in order to construct ideal farm houses in rural area. The results obtained were as follows: 1. In heat conservation due to construction of walls the thermal efficiency of cement brick house was equivalent to 66.3% of that of soil brick house, and boulder house 60.3% 2. In the case of ceiling, the thermal efficiency of paper ceiling was amounted to 84.2% of that of the composite ceiling (thickness 6mm veneer+thickness. l0m chaffs), and the common ceiling putting on soil above the ceiling, 76% of the composite while the efficiency of the ceiling putting on chaffs above them was 15.8% higher than that of the paper. 3. In the case of improving the window, the double type was 12% higher than. the efficiency of single type. 4. The warming velocity of conventional house was slower but the velocity of radiation was quicker than that of experimental one. It was thought to be due to unscietific constructions of the room bottom, fire inlet and chimney, 5. The temperature gradient line was not dependad upon the amount of throwing into fuel in the rural farm house. 6. It was concluded that the final thermal efficiency of the conventional farm house was 10.6% lower than that of experimental farm house.

• International Journal of Automotive Technology
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• 제6권2호
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• pp.95-105
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• 2005

Diesel engines have low specific fuel consumption, but high particulate emissions, mainly soot. Diesel soot is suspected to have significant effects on the health of living beings and might also affect global warming. Hence stringent measures have been put in place in a number of countries and will be even stronger in the near future. Diesel engines require either advanced integrated exhaust after treatment systems or modified engine models to meet the statutory norms. Experimental analysis to study the emission characteristics is a time consuming affair. In such situations, the real picture of engine control can be obtained by the modeling of trend prediction. In this article, an effort has been made to predict emissions smoke and NO$_{x}$ using cylinder combustion derived parameters and diesel particulate filter data, with artificial neural network techniques in MATLAB environment. The model is based on three layer neural network with a back propagation learning algorithm. The training and test data of emissions were collected from experimental set up in the laboratory for different loads. The network is trained to predict the values of emission with training values. Regression analysis between test and predicted value from neural network shows least error. This approach helps in the reduction of the experimentation required to determine the smoke and NO$_{x}$ for the catalyst coated filters.

• 동력기계공학회지
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• 제18권6호
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• pp.106-112
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• 2014

The diesel engine, which has high compression ratio than other heat engines, has been using as the main power source of marine transport. Especially, since marine diesel engines offer better specific fuel consumption (SFC), it is environment-friendly compared to those used in other industries. However, attentio should be focused on emissions such as nitrous oxide ($N_2O$) which is generated from combustion of low-grade fuels. Because $N_2O$ in the atmosphere is very stable, the global warming potential (GWP) of $N_2O$ is 310 times as large as that of $CO_2$, and it becomes a source of secondary contamination after photo-degradation in the stratosphere. It has been hitherto noted on the $N_2O$ exhaust characteristics from stationary power plants and land transportations, but reports on $N_2O$ emission from the marine diesel engine are very limited. In this experimental study, a author investigated $N_2O$ emission characteristics by using changed diesel fuel components of nitrogen and sulfur concentration, assessed on the factors which affect $N_2O$ generation in combustion. The experimental results showed that $N_2O$ emission exhibited increasement with increasing of sulfur concentration in fuel. However, all kinds of nitrogen component additives used in experiment could not change $N_2O$ emission.

• 한국임상수의학회지
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• 제40권1호
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• pp.44-49
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• 2023

The purpose of this study was to evaluate body temperature changes in rabbits anesthetized using water-filtered infrared-A (wIRA). Ten rabbits were used for this study. For the experimental group (wIRA group; wG, n = 5), the experimental equipment was used and irradiated using wIRA. The control group (CG, n = 5) did not have any warming device. There were no significant differences in heart rate, respiration rate, and end tidal CO₂ (EtCO₂) between wG and CG. After 80 min, the core body temperature of wG rabbits was significantly higher than that of CG rabbits. The surface body temperature was significantly higher while receiving wIRA support at all time points after 5 min. In conclusion, in rabbits under inhalation anesthesia, the surface body temperature was better maintained than the core body temperature when using wIRA.

• 한국태양에너지학회 논문집
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• 제26권2호
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• pp.19-25
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• 2006

Methane hydrate, non-polluting new energy resource, satisfies requirement and considered as a precious resource that can prevent the global warming. Fortunately, there are abundant resources of methane hydrate distribute in the earth widely. Therefore, developing the techniques that can utilize these gases effectively is highly desired. The work in this paper here is to develop a skill which can transport and store methane hydrate. As a first step, the equilibrium experiment was carried out by increasing temperatures in the cell at fixed pressures. The influence of gas consumption rates under variable degree of subcooling, stirring and water injection has been investigated formation to clarify kinetic characteristics of the hydrate. The results of present investigation showed that the enhancements of the hydrate formation in terms of the gas/water ratio are closely related to operational pressure, temperature, degrees of subcooling, and water injection.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.370.1-370.1
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• 2016

Volatile organic compounds (VOCs) are considered hazardous air pollutants and these are emitted from building materials and household products. VOCs can cause global warming as well as human sickness, and even cancer. Photocatalysis provides a way of converting VOCs into harmless materials. Various researches have shown that $TiO_2$ is the most efficient photocatalysts due to its excellent activity. In this study, metal/non-metal doped $TiO_2$ particles are synthesized for the enhancement of the photocatalytic properties of pure $TiO_2$. By metal/non-metal doping, band gap energies of prepared samples were analyzed by UV/Visible spectrophotometer. The physical and chemical properties of synthesized powder were characterized by field emission scanning electron microscope, by BET for measuring their specific surface area, and by XRD for phase identification and particle size determination. Degradation ability for p-xylene was evaluated through monitoring the concentration in a closed chamber. Relation between their properties and decomposition abilities for VOC were evaluated based on the experimental results.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.703-704
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• 2009

Natural gas Hydrate is an ice-like crystal containing natural gas it. Natural gas hydrate is studied as a new energy resource and a factor for seafloor slope stability and global warming. The unique pressure and temperature stability conditions of natural gas hydrate have challenged the research efforts. In this study, a new tool to study hydrate-bearing sediments and the preliminary results are introduced. The device can sustain 20MPa of the fluid pressure and apply 5MPa of the vertical effective stress under the temperature control. Cell can be scanned by X-ray CT scanner and also has the capability of multi-sensor data acquisition. Preliminary results suggests various application of the cell to hydrate-bearing research.