• Clinical and Experimental Pediatrics
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• v.46 no.2
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• pp.173-177
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• 2003

Purpose : Recently, different results about factors affecting accurate quantitation of 24-hr urinary protein(24UP) amount using spot urine protein/creatinine ratio(PCR) have been reported. The current study was designed to evaluate correlation between 24UP amounts and PCR in children, and the effect of 24UP amounts, age, sex, and glomerular filtration rate(GFR) on this correlation. Methods : Among 94 patients who visited the department of pediatrics in Busan Paik Hospital from March 2002 to August 2002, 68 patients whose urinary creatinine excretion was ${\geq}15mg/kg/day$ were included in this study. All the patients were divided into I, II/A, B group(I : 24UP<500 mg/day, II : $24UP{\geq}500mg/day$, A : <10 years of age, B : ${\geq}10years$ of age). Pearson correlation analysis was performed between 24UP and PCR to evaluate the relationship. We defined fractional difference between 24UP and PCR, and then performed multiple regression analysis with 24UP amount, age, GFR and fractional difference. Results : There was a strong positive linear correlation between 24UP and PCR(R=0.936, P<0.0001) in all patients, and the correlation was also good in each group. Using PCR cutoff values of 0.5, the PCR provided high sensitivity, specificity, positive and negative predictive value in predicting 24UP amount ${\geq}500mg$. The factors affecting accurate quantitation of proteinuria using spot urine PCR was age, not 24UP amount, GFR or sex. Conclusion : Spot urine PCR is a useful test but has limitations in predicting 24UP amount. Therefore, it should be used only as screening method. Age-adjusted PCR cutoff values may be necessary to predict 24UP amount in children with proteinuria.

• Analytical Science and Technology
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• v.28 no.6
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• pp.377-384
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• 2015

A survey of the artificial radionuclides in rivers and lakes was conducted to investigate their levels in surface water. Water samples were collected at 60 points and analyzed by gamma-ray spectrometry with a measurement time of 10,000 seconds for ¹³⁴Cs, ¹³⁷Cs, and ¹³¹I. The obained values were lower than MDA for all points, except one point for ¹³¹I that was 0.533±0.058 Bq/L. ¹³¹I is known as a radioactive material that occurs frequently in sewage treatment plants. Because it is often used for medical treatments and subject to spreading into the environment due to the excretion from the patients. For the point where ¹³¹I was detected, we conducted additional investigation on the upstream river point and the effluent points of nearby sewage treatment plant to find the source of ¹³¹I. ¹³¹I was not detected at the upstream points of one of the upstream sewage treatment plants but found at the downstream points with the level being 0.257±0.034 to 0.799±0.051 Bq/L, proving the sewage treatment plant was the ¹³¹Isource.

• Nutritional Sciences
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• v.6 no.4
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• pp.223-228
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• 2003

This study was carried out to investigate the effects of vitamin E on the cadmium contents of bone and on the calcium and phosphorous contents of the blood, urine and feces. Male Sprague-Dawley rats weighing 100$\pm$10g were randomly assigned to one normal group and three cadmium poisoned groups. The cadmium poisoned groups consisted of a vitamin E free diet (Cd-0E) group; a 40 mg vitamin E /kg diet (Cd-40E) group; and a 400 mg/kg diet (Cd-400E) group. Experimental animals were maintained on their respective diets for 20 weeks and were simultaneously administered 50 ppm $Cd^{2+}$ dissolved in the drinking water. At the end of the trial, the average hematocrit value in the Cd-0E group was 28.13% lower than in the normal group. However, the average hematocrit value in the Cd-400E group was significantly higher than in the Cd-0E and Cd-40E groups. WBC levels in the cadmium-poisoned groups were lower than in the normal group, but Cd-400E group levels were significantly higher than in the Cd-0E and Cd-40E groups. The contents of calcium of tibia has no significant difference between normal group and cadmium exposed group at $10^{th}$ week After 20 weeks, the calcium contents of the tibia in the Cd-0E and Cd-40E groups were lower than in the normal group by 25.5% and 22.1 %, respectively, although the calcium contents of the tibia in the Cd-400E group were higher than in the normal group. After 10 weeks, the calcium contents of the femur in the Cd-0E and Cd-40E groups were 19.25% and 15.45% lower than in the normal group, respectively, but the calcium contents of the femur in the Cd-400E group were at the same levels as in the normal group. The levels of calcium in the femur after 20 weeks were similar to the 10-week levels. Calcium levels of the urine in the Cd-0E and Cd-40E groups were 3.92 fold and 2.92 fold higher, respectively, than in the normal group, but levels in the Cd-400E group were significantly lower than in either the Cd-0E group or the Cd-40E group. Calcium levels of the feces in cadmium-poisoned groups were significantly higher than in the normal group, although levels in the Cd-400E group were significantly lower than in the Cd-0E and Cd-40E groups. Phosphorous levels of the blood in the Cd-0E group were 17% lower than in the normal group, although levels in the Cd-400E group were significantly higher than in the Cd-0E group. Phosphorous levels of the urine in the Cd-0E and Cd-40E groups were significantly higher than in the normal group, while Cd-400E group levels were found to be at the same level as in the normal group. Cadmium contents of the tibia in the Cd-40E and Cd-400E groups were 13% and 17% lower, respectively, than in the Cd-0E group. Regarding cadmium levels in the femur, only the Cd-400E group achieved lower levels (10% lower) than the Cd-0E group. In conclusion, vitamin E supplementation resulted in a suppression of the release of calcium from bone, and a reduction in the excretion of calcium via the urine and feces, thus having a normalizing effect on calcium metabolism in rats with chronic cadmium poisoning.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.5
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• pp.677-683
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• 2010

This study investigated the effect of young Phragmites communis (Pc) leaves on lipid profiles, lipid metabolism and erythrocyte antioxidant defense system in high-fat diet fed mice. Three groups of mice were fed different diets for 8 weeks: normal diet (Normal), high-fat diet (High-fat; 37% calories from fat) and high-fat diet supplemented with 1% Pc (wt/wt, HF-Pc). Body weight, daily food intake and energy intake tended to decrease by Pc supplement in high-fat fed mice. Pc supplementation significantly lowered plasma triglyceride and total cholesterol concentrations compared to the high-fat control group. Pc also lowered hepatic and heart cholesterol contents, whereas it significantly increased fecal excretion of triglyceride and cholesterol compared to the high-fat control group. Pc significantly inhibited fatty acid synthase, 3-hydroxy-3-methylglutaryl CoA reductase and acyl-CoA:cholesterol acyltransferase activities compared to the high-fat control group. Erythrocyte superoxide dismutase and catalase activities were also significantly higher in the high-fat group than in the normal group, however Pc supplementation reversed these changes. The Pc supplementation significantly lowered erythrocyte lipid peroxidation level compared to the high-fat control group. Accordingly, these results suggest that Pc improves lipid metabolism and erythrocyte antioxidant defense system in high-fat diet fed mice.

• Journal of Nutrition and Health
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• v.41 no.7
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• pp.602-611
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• 2008

We compared antiobese, hypocholesterolemic, antiplatelet and antioxidant effect of 10% green tea powder and 3% green tea extract in rats pair fed 5% cholesterol diets. The final body weight was decreased significantly compared with the control (p < 0.05). Plasma and liver total cholesterol were lower in group of green tea powder or extract, but not statistically different. HDL cholesterol was increased significantly in group of green tea powder compared with the control or green tea extract (p < 0.05). Plasma triglyceride was significantly decreased in group of green tea extract compared with green tea powder, and green tea powder compared with the control respectively (p < 0.05). Liver triglyceride was significantly decreased in group of green tea powder or green tea extract compared with the control (p < 0.01). Platelet aggregations in the maximum and initial slope were not different among groups. Hemolysis was significantly lower in group of green tea powder compared with the control (p < 0.05). Plasma TBARS production was decreased in group of green tea extract compared with the control (p < 0.05). Na passive leak in intact cells was not different, but Na leak in AAPH treated cell was significantly decreased in group of green tea powder than the control (p < 0.05). The leak increase (${\Delta}Na$ Leak) after AAPH treatment was significantly decreased in groups of green tea powder and extract compared with the control (p < 0.05). Isotope excretion after $^{14}C$-cholesterol ingestion was significantly increased in group of green tea extract compared with the control or the green tea powder (p < 0.05). Consumption of green tea in powder or extract may give beneficial effects in weight control and plasma lipid profiles, impeding metabolic syndrome. More studies are needed to clarify what component of green tea and what mechanism are involved in antiobese and hypolipedemic actions of green tea.

• Journal of the korean academy of Pediatric Dentistry
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• v.27 no.1
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• pp.62-69
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• 2000

Part of the locally applied, as well as the systemic applied, fluoride is absorbed into the body to aid in the prevention of caries. However, beyond a certain level, systemic distribution of fluoride can cause chronic fluorosis with attending systemic symptoms and dental fluorosis. Thus it is vital to determine the level of fluoride with minimal side effects which will provide optimal caries prevention. A commonly utilized method of regressively determining fluoride intade is to measure the fluoride concentration of excreted urine. Thus, the aim of this study was to determine the clearance time and concentration of fluoride in urine after administration of various doses of fluoride using HMDS-diffusion technique and fluoride ion electrode(Orion, 96-09, U.S.A.). Urine samples were collected in 7 adult subjects every morning after administration of fluoride supplements such as no fluoride(control group), 1mg fluoride(group 1), 2mg fluoride(group 2), 3mg fluoride(group 3), 4mg fluoride(group 4). The obtained results were as follows 1. Mean urinary fluoride concentration of control group was $0.707{\pm}0.362ppm$. 2. Fluoride levels followed as group 4(4.076ppm). group 3(2.400ppm), group 2(1.494ppm), group 1(1.051ppm) at day 1 after fluoride administration. There were no statistical differences between the urinary fluoride concentration of group 1, 2, 3 and control group after day 2, but there was statistical difference between group 4 and control group at day 2(p<0.05). 3. Urinary fluoride concentration increased and plateaued according to increasing fluoride dosage. The increased concentration remained significantly higher till day 2, but after day 3, there was no significant difference compared to the control.

• Journal of Life Science
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• v.15 no.5 s.72
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• pp.790-795
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• 2005

The effects of liquid culture of Coriolus (C) versicolor on weight gain, food intakes, food efficiency ratios, serum and hepatic lipid concentrations were investigated in male rats fed the high cholesterol diets. Eight weeks old Sprague-Dawley rats were given three different types of diet for six weeks, respectively: a control diet ($ 20\% fat +0.5 \% $ cholesterol), two kinds of C versicolor diet (control diet + $ 30\% or 40\%$C. versicozor in water) according to the levels of C. versicolor supplementation. The body weight gains of the rats fed $ 30\% or 40\% $ C. versicolor diets were lower than those in the rats fed the control diet. The food intake, food efficiency ratios, and liver, kidney, epididymal fat pad weights of the rats fed $ 30\% or 40\%$ C. versicoEer diets were similar to those of the rats fed the control diet. The concentrations of hepatic cholesterol and triglyceride in the rats fed $ 30\% or 40\% $ C. versicolor diets were significantly lower than those in the rats fed the control diet. The concentrations in serum total cholesterol, LDL-cholesterol, and atherogenic index ratios were significantly lower in the rats fed $ 30\% or 40\%$ C. versicolor diets compared to those fed the control diet. The HDL-cholesterol/total-cholesterol ratios was significantly higher in the rats fed $ 30\% or 40\% $ C. versicolor diets compared to those fed the control diet. The fecal excretion of total lipid and triglyceride in the rats fed $ 40\% $ C. versicolor diet was significantly higher than that of the rats fed the control diet. There were no significant difference found in the serum trigly-ceride, phospholipid and HDL-cholesterol concentrations among the experimental groups. These results showed that the C. versicolor feeding decreased the hepatic cholesterol, triglyceride, and the serum total cholesterol, LDL-cholesterol and atherogenic index, and increased the serum HDL-cholesterol/ total-cholesterol ratio of the rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.1
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• pp.17-25
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• 2013

This study was performed to investigate the effects of the hot water extract of red garlic (RG) and RG-composites on fecal lipid levels and hepatic antioxidant enzyme activity in rats fed a high fat-cholesterol diet. Three different types of RG-composites prepared: RG and green tea (RGT), RG and dietary fiber (RGF), and RG, green tea, and dietary fiber (RGTF). Rats were divided into six groups: the control, the group fed a high fat-cholesterol diet (HFC), the RG-supplemented group (HRG), the RGT supplemented group (HRGT), the RGF supplemented group (HRGF), and the RGTF supplemented with HFC group (HRGTF). The antioxidant activity of these composites was tested, in vitro. The DPPH radical scavenging activity was higher RGT and RGTF than RG. ABTs radical scavenging activity of RGT was similar to RGTF. Their activities were significantly higher than RG. The reducing power was similar to their radical scavenging activities. Total lipid levels in the liver and triglyceride levels in the heart were lower in the groups fed RG-composites than the HRG group. Fecal total lipid level was higher in the HRGF and HRGTF groups than the HRG group after 4 weeks diet supplementation during 4 weeks. Lipid peroxide content was reduced to anywhere between 6.2% and 12.1% in the groups fed RG-composites, compared to the HFC group. Antioxidant activity was significantly higher in the groups fed RG-composites than the HFC group. Hepatic SOD activity was higher in the groups fed RG-composites than the HRG group. The HRGT group in catalase activity, and the HRGT and HRGTF groups in GSH-px activity were increased significantly compared to the HFC group. Hepatic UDPGT activity was increased significantly in the HRGT and HRGTF groups to the HFC group, as well. These results indicate that antioxidant activities of the RG-composites were related to the decrease of lipid levels by increasing the fecal excretion and enhancement of hepatic antioxidant enzyme activity in rats fed a high fat-cholesterol diet.

• Journal of Mushroom
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• v.16 no.2
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• pp.118-124
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• 2018

In this study, we aimed to investigate the effects of dietary supplementation with fruiting body of Agaricus brasiliensis (AB) mushroom on the lipid profiles of serum and histological patterns of liver of high cholesterol-fed rats. Five-week-old, female Sprague-Dawley albino rats were divided into three groups of 8 rats each, including a normal control-diet (NC) group, a high-cholesterol diet (HC) group, and a group fed high-cholesterol diet supplemented with 5 % powder of Agaricus brasiliensis fruiting bodies (HC+AB). Total serum cholesterol, low density lipoprotein (LDL), and triglyceride (TG) concentrations in the HC+AB group were significantly reduced when compared with those in the HC group. Body weight in the HC+AB group was significantly lower than that in the HC group, whereas no adverse effects were observed on the levels of plasma albumin, creatinine, blood urea nitrogen, uric acid, glucose, and total protein. In the HC+AB group, liver enzyme activities related to liver function, such as GOT and GPT, presented values lower than those in the HC group and were very similar to the ones in the NC group. Excretion of total lipid and cholesterol in feces in the HC+AB group was significantly higher than that in the NC and HC groups, indicating that mushroom feeding inhibits the absorption of lipid cholesterol in the intestine. Liver histopathological analyses revealed that rats fed with HC diet developed fat liver disease, whereas only small amounts of fat were deposited in the livers of the HC+AB group. In conclusion, the results suggest that fruiting body powder of A. brasiliensis provides health benefits to high-cholesterol-fed rats by lowering body weight and the risk of atherogenic lipid profile.

• Journal of Animal Science and Technology
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• v.46 no.1
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• pp.39-48
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• 2004

This experiment was conducted to investigate the effect of continuous feeding of probiotics on growth performance, nutrient digestibility, blood urea nitrogen(BUN) and immune responses in pigs. Treatments were 1) Control(basal diet), 2) P-O.l(basal diet + 0.1% probiotics) and 3) P-0.2(basal diet + 0.2% probiotics). In growth trial, a total of sixty pigs(6.17 $\pm$ 0.45 kg average body weight) weaned at 21 days of age were used. All pigs were assigned according to sex and body weight, and each treatment had 5 replicates of 4 pigs per pen in a randomized complete block(RCB) design. During 0${\sim}$8 weeks, there was no significant difference in average daily gain(ADG), average daily feed intake(ADFI) and gain:feed ratio(GfF) among treatments. During 9 - 20 weeks, ADG was improved significantly in pigs fed P-O.I or P-0.2 diets when compared to the pig fed control diet(P <0.05), but there was no significant difference in ADFI and GfF ratio. During overall period, ADG, ADFI and GfF ratio were not significantly different among treatments. In the first metabolic trial(17.93 $\pm$1.45kg average body weight), apparent digestibility of OM, protein, fat in pigs fed P-O.l and P-0.2 diets were greater than in pigs fed control diet(P <0.05) and ash digestibility in pigs fed P-0.2 diet was significantly higher than in pigs fed control diet(P <0.05). Calcium digestibility in pigs fed P-0.2 diet was significantly higher than in pigs fed control and P-O.I diets(P <0.05). Fecal-N excretion was lower in pigs fed P-O.! and P-0.2 diets than in pigs fed control(P <0.05). In the second metabolic trial(41.80 $\pm$ 2.68kg average body weight), there was no significant difference among treatments in apparent digestibility of nutrients and N-retention. In blood assay for the BUN and immune responses investigations, there was no significant difference among treatments during overall period of experiment. Therefore, this experiment suggested that probiotics supplementation could improve growth performance and nutrient digestibility of pigs.