• Parasites, Hosts and Diseases
• /
• 제56권5호
• /
• pp.521-525
• /
• 2018

Rodents are well-known reservoirs and vectors of many emerging and re-emerging infectious diseases, but little is known about their role in zoonotic disease transmission in Bhutan. In this study, a cross-sectional investigation of zoonotic disease pathogens in rodents was performed in Chukha district, Bhutan, where a high incidence of scrub typhus and cases of acute undifferentiated febrile illness had been reported in people during the preceding 4-6 months. Twelve rodents were trapped alive using wire-mesh traps. Following euthanasia, liver and kidney tissues were removed and tested using PCR for Orientia tsutsugamushi and other bacterial and rickettsial pathogens causing bartonellosis, borreliosis, human monocytic ehrlichiosis, human granulocytic anaplasmosis, leptospirosis, and rickettsiosis. A phylogenetic analysis was performed on all rodent species captured and pathogens detected. Four out of the 12 rodents (33.3%) tested positive by PCR for zoonotic pathogens. Anaplasma phagocytophilum, Bartonella grahamii, and B. queenslandensis were identified for the first time in Bhutan. Leptospira interrogans was also detected for the first time from rodents in Bhutan. The findings demonstrate the presence of these zoonotic pathogens in rodents in Bhutan, which may pose a risk of disease transmission to humans.

• 대한수의학회지
• /
• 제40권3호
• /
• pp.575-585
• /
• 2000

This study was performed to observe the influence of host immune response on the chemotherapy of mice experimentally infected with Fasciola hepatica. Following immunosuppression with prednisolone or immunoenhancement with Freund's complete adjuvant(FCA), mice were experimentally infected with 3 Fasciola hepatica metacercariae and treated with closantel at 1 week post infection. In the group of mice infected with metacercariae alone, 2 mice of 10 were dead at 10 weeks post infection(20% mortality), and adult flukes were recovered from the liver and the peritoneal cavity of the remaining 8 mice(100% infectivity). In the group of mice treated with prednisolone and infected with metacercariae, 8 of 10 mice died before euthanasia with a mean time of death earlier than the control group (p<0.05). In the group of immunosuppressed mice infected with metacercariae and treated with closantel 20mg/kg, 4 of 10 mice died before sacrifice. In the group of mice infected and treated with closantel 20mg/kg, mortality and infectivity was 10% and 30%, respectively. Similar results were observed in mice infected and treated with closantel 5mg/kg which resulted in 10% and 50% mortality and infectivity, respectively. These results indicated that the efficacy of closantel treatment was decreased in immunosuppressed mice, while the pathogenicity was increased. In immunoenhanced mice infected with metacercariae, on the other hand, the efficacy of chemotherapy with both 5mg/kg or 20mg/kg closantel resulted in only 10% infectivity. The results shown in this study strongly suggest that a close interaction between chemotherapy against F hepatica with closantel and the host immune system exists. Considering that fascioliasis is a zoonosis, treatment regimen against the infection to immunosuppressed patients may require a concurrent prescription of an appro-priate immuno-enhancing adjuvant.

• 한국콘텐츠학회논문지
• /
• 제17권4호
• /
• pp.500-509
• /
• 2017

본 연구는 일반 대학생의 생명의료윤리의식과 공감능력의 정도를 평가하고 그 연관성을 살펴보고자 하였다. 연구대상은 지역대학생 469명이었다. 연구 결과로서 대학생의 생명윤리의식 정도는 2.89점이었고 그중 신생아 생명권과 태아의 생명권이 각각 3.32, 3.20점으로 가장 높았다. 대학생의 공감능력은 2.41로 중간 수준이었다. 생명의료윤리의식은 공감과 정적상관관계를 보였고, 생명윤리의 하위요인 중 태아의 생명권, 인공임신 중절, 인공 수정, 태아 진단, 신생아의 생명권이 인지적 공감 및 정서적 공감과 정적 상관관계를 나타내었다. 또한 공감 능력 중 정서적 공감이 생명윤리의식에 영향을 나타내었으며, 정서적 공감 중 공감적 관심 요인이 생명윤리의 하위 요인에서 태아의 생명권, 인공임신 중절, 인공수정, 태아진단, 신생아의 생명권, 안락사, 뇌사 요인에 영향을 미치는 것으로 나타났다. 향후 대학생들에서 공감과 관련된 생명윤리의식 교육이 필요할 것으로 생각된다.

• 한국임상수의학회지
• /
• 제34권5호
• /
• pp.341-346
• /
• 2017

This study was performed to evaluate the effects of an intravitreal injection of cidofovir in beagle dogs. Nine beagle dogs (18 eyes) were used and randomly assigned to the following three groups of various dosages: 100, 500 and $1000{\mu}g$. Aqueous paracentesis was followed by an intravitreal injection of cidofovir. Intraocular pressure (IOP) was measured twice a week and electroretinography (ERG) and ophthalmoscopic examination were performed every week during the study. At the end of the study, all eyes were enucleated for histopathologic evaluation after euthanasia. The IOPs in the 500 and $1000{\mu}g$ groups were statistically lower than baseline with no significant IOP changes in the $100{\mu}g$ group. Reduced amplitudes of ERG recordings were identified in the eyes injected with higher dose groups than the $100{\mu}g$ group. Histopathologic examination revealed that there were dose-related toxicities to the ciliary body and the retina. These results suggest that intravitreal cidofovir had dose-dependent IOP lowering effects associated with ciliary body destruction, but had the potential to cause retinal toxicity in beagle dogs.

• 디지털융복합연구
• /
• 제11권4호
• /
• pp.311-320
• /
• 2013

본 연구는 일 대학 간호과와 비 간호과 학생의 생명의료윤리 의식을 파악하여 대학생들의 올바른 생명윤리 의식 함양에 필요한 윤리교육의 방향과 교육과정 내용을 위한 기초자료를 제공하고자 시도하였다. 생명의료윤리 의식은 4점 만점에 간호과 학생들이 평균 3.04점으로 비 간호과 학생들의 2.88점보다 높았고 이들 간에 유의한 차이가 있었다(t=6.79, p<.001). 두 그룹간에 태아의 생명권, 태아진단, 신생아 생명권, 안락사, 장기이식, 뇌사영역에서 통계적으로 유의한 차이를 나타내었다. 대상자들의 생명의료윤리 의식에 영향을 미치는 주요 요인은 간호과에서는 한 학기 이상 윤리관련 수업 경험 유무, 생명윤리 관련 문제로 고민한 경험 유무, 윤리적 가치관이었고, 비 간호과는 학년으로 나타났다. 결과적으로 대상자들의 낮은 생명의료윤리 의식은 각 학과별로 기본적인 생명윤리 교육부터 고학년으로 올라갈수록 체계적이고 복합적인 윤리적 사고 판단과 의사결정 능력, 가치관 고취를 위한 교육과정을 통해 충족되어야 할 것이다. 추후 생명의료윤리 의식의 하위영역별 집중연구나 질적 연구를 제언한다.

• Toxicological Research
• /
• 제20권3호
• /
• pp.233-239
• /
• 2004

The present study was designed to determine the impact of mercury on endotoxin-induced inflammatory cytokine expression and corresponding signal transduction in mouse kidney. Male BALB/c mice were exposed continuously to 0, 0.3, 1.5, 7.5, or 37.5 ppm of mercury in drink-ing water for 14 days and at the end of the treatment period, lipopolysaccharide (LPS, 0.5 mg/kg) was injected intraperitoneally 2 h prior to euthanasia. The doses of mercury and LPS did not cause hepatotoxicity or renal toxicity as indicated by unaltered plasma alanine aminotransferase and aspartate aminotransferase levels, and terminal UTP nucleotide end-labeling assay from kidney, respectively. Mercury decreased kidney glutathione (GSH) and with LPS, it additively decreased GSH. Mercury activated p38 mitogen-activated protein kinase (MAPK) and additively increased LPS-induced p38 MAPK phosphorylation. In contrast, mercury inhibited LPS-induced activation of extra-cellular signal-regulated kinase (ERK) but had no effect alone. Mercury increased the gene expression of tumor necrosis factor $\alpha$ (TN F$\alpha$) and potentiated LPS-induced TNF$\alpha$ expression. Mercury did not affect LPS-induced interleukin-1$\beta$ (IL-1$\beta$) expression but decreased LPS-induced IL-6 expression. These results suggest that low levels of mercury might augment LPS-induced TNF$\alpha$ expression by altering GSH and p38 MAPK. Mercury modulates LPS-induced p38 and ERK activation, and downstream TNF$\alpha$ and IL-6 expression in kidney, respectively.

• 한국임상수의학회지
• /
• 제29권3호
• /
• pp.250-254
• /
• 2012

배뇨곤란, 혈뇨 및 간헐적인 구토를 보이는 8살의 중성화된 수컷 슈나우져가 병원에 내원하였다. 투시기 하에서 양성 요도 조영을 실시 하였고, 전립선 주위 요도에서 조영제가 누출 되는 것을 확인 하였다. 또한 전립선 요도부터 방광 삼각까지의 충만 결손이 관찰 되었다. 수술적인 교정이 부분적인 방광 폐쇄로 인해 실시 되었고 수술 중 주변 장기로 전이 소견이 확인되어 안락사를 실시 하였다. 주변 조직의 전이를 확인 하고 위하여 Cytokeratins (AE1/AE3) 를 이용하여, 주위 림프 조직을 염색하여, 전립선의 상피 세포 유래의 세포가 림프절에 전이 되었음을 확인 하였다. 본 증례는 수컷 개의 전립선 선암종의 발생 증례로 임상 소견, 영상 진단, 조직 병리학적인 자료를 기술 하였다.

• 한국식품영양학회지
• /
• 제23권1호
• /
• pp.124-133
• /
• 2010

The total number of the dogs bred in Korea as of 2007 was 1,917,709, and among them, 77%, 1,476,776 dogs were edible dogs. Dogmeat has been legalized edible as food from Choseon dynasty, Daehan imperial state, Japan-occupied era till the present Korea. Dogs had been included in the article 2, Enforcement Ordinance of Processing and Disposal Rule of Livestock and Its Products until the end of Jan. 1979, but it was crossed out by the Notification No 3,005(Feb. 1 1979 effective) of the Minister of Agriculture and Marine Products, and as a result, the obligation that dogs should be slaughtered at the slaughtering ground was defunct. Thus, the arbitrarily dog slaughtering was empowered. As a matter of fact, the new law was not legalized in order to ban dogs from being slaughtered. The waste amount of slaughtered edible dogs amounts to 7,282 tons annually, and most of its waste from the arbitrarily-slaughtered dog is being illegally dumped without proper management and supervision. Edible dogs defecate 292,509 tons(calculates urine as dung) annually, but it is sanitarily disposed according to the Law of Management and Use of Livestock's Dung and Urine which took effective from Sep. 2009. Annual sales amount of edible dogs comes to 590 billion won on the basis of the shipment at breeding ground, but after passing through various level of marketing, and being processed as Gaesoju, and Boshintang, it forms 4 trillion won market when it reaches customers. The amount of food waste in Korea in 2007 came to 5,274,944 tons, and 633 billion won was spent for its disposal cost. Korean edible dogs of 1,476,776 heads consumed 1,266,705 tons, the 24% of total food waste. Edible dogs are the most effective means to convert food waste into food for man, not entailing the cost of disposal. On the other hand, pet dog culture brought about disposal cost, and the 51,188 dogs were abandoned at 2007, while 7 billion won was spent for the protection, euthanasia of them and the disposal of their dead bodies.

• 한국간호교육학회지
• /
• 제24권3호
• /
• pp.214-224
• /
• 2018

Purpose: This study examines the effectiveness of an educational program of nursing biomedical ethics for nursing students to increase awareness of the Code of Ethics for Nurses, moral sensitivity, and application of the Code of Ethics for Nurses. Methods: A quasi-experimental research design and t-test were used. The experimental group (n=31) and control group (n=32) were second-year university nursing students in two major cities. The experimental group received 15 hours of nursing ethics education during the 15-week semester. The training was not provided to the control group. Results: The application of the Code of Ethics for Nurses increased significantly in the experimental group (t=-1.06, p=.017), nurses and patient (t=-2.23, p=.029), obligation of nurse as an expert (t=-2.08, p=.042), nurse and cooperator (t=-2.54, p=.014). The consciousness of biomedical ethics increased significantly in the experimental group (t=4.28, p=.021), newborn's right to live (t=-2.61, p=.011), euthanasia (t=-2.36, p=.021). Conclusion: The results of the study show that providing a nursing biomedical ethics program to nursing students is an effective method to enhance the application of the Code of Ethics for Nurses and Consciousness of Biomedical Ethics. Implementing an intervention program of the Code of Ethics for Nurses in the regular nursing curriculum may reduce conflicts involving ethical decision making by nurses.

• 지역사회간호학회지
• /
• 제29권2호
• /
• pp.244-256
• /
• 2018

Purpose: This study tried to understand discourses of life-sustaining treatments in general daily and healthcare newspapers. Methods: A text-network analysis was conducted using the NetMiner program. Firstly, 572 articles from 11 daily newspapers and 258 articles from 8 healthcare newspapers were collected, which were published from August 2013 to October 2016. Secondly, keywords (semantic morphemes) were extracted from the articles and rearranged by removing stop-words, refining similar words, excluding non-relevant words, and defining meaningful phrases. Finally, co-occurrence matrices of the keywords with a frequency of 30 times or higher were developed and statistical measures-indices of degree and betweenness centrality, ego-networks, and clustering-were obtained. Results: In the general daily and healthcare newspapers, the top eight core keywords were common: "patients," "death," "LST (life-sustaining treatments)," "hospice palliative care," "hospitals," "family," "opinion," and "withdrawal." There were also common subtopics shared by the general daily and healthcare newspapers: withdrawal of LST, hospice palliative care, National Bioethics Review Committee, and self-determination and proxy decision of patients and family. Additionally, the general daily newspapers included diverse social interest or events like well-dying, euthanasia, and the death of farmer Baek Nam-ki, whereas the healthcare newspapers discussed problems of the relevant laws, and insufficient infrastructure and low reimbursement for hospice-palliative care. Conclusion: The discourse that withdrawal of futile LST should be allowed according to the patient's will was consistent in the newspapers. Given that newspaper articles influence knowledge and attitudes of the public, RNs are recommended to participate actively in public communication on LST.