• International Journal of Oral Biology
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• 제35권4호
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• pp.177-184
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• 2010

In our present study, we investigated the effects of continentalic acid on Streptococcus mutans (S. mutans) biofilm. Methanol extract of Aralia continentalis (A. continentalis) was suspended in water and sequentially partitioned with CHCl3, ethyl acetate (EtOAc), and n-butanol (n-BuOH). The CHCl3 fraction showed the highest activity and an antibacterial compound against S. mutans was isolated from this preparation through various chromatography methods by bioassay guided fractionation. MS, $^1H-NMR$ and $^{13}C-NMR$ analysis showed that the active principle was continentalic acid which was confirmed to show significant inhibitory effects against S. mutans biofilm. These results may provide some scientific rationale for the traditional use these extracts for the treatment of dental diseases.

• 약학회지
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• 제39권6호
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• pp.610-615
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• 1995

Three acidic triterpense were isolated from the ethyl acetate soluble fraction of the fruits of Chenomeles sinensis (Rosaceae) together with previously reported oleanolic acid and ursolic acid by repeated silica gel colunm chromatography. Their structures have been elucidated as $2{\alpha},{\;}3{\betha}-dihydroxyolean-l2-en-28-oic$ acid(l. maslinic acid), $2{\alpha},{\;}3{\alpha},{\;}19{\alpha}-trihydroxy-urs-12-en-28-oic$ acid (2, euscaphic acid), and $2{\alpha},{\;}3{\betha}-dihydroxyolean-l2-en-28-oic$ acid (3,tormentic acid), respectively, on the basis of chemical and spectral evidence.

• Archives of Pharmacal Research
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• 제29권5호
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• pp.343-347
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• 2006

A new flavanone Persinol (1) and the new flavanone glucosides persinosides A (2) and B (3), along with known flavonoids (4 and 5) have been isolated from the ethyl acetate soluble fraction of the whole plants of Aerva persica. Their structures were elucidated on the basis of extensive analysis of nuclear magnetic resonance (1D & 2D-NMR) spectral data. All of them showed profound antioxidative activities by DPPH and cytochrome-c-reduction assays using the HL-60 cell culture system.

• 약학회지
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• 제45권5호
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• pp.522-528
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• 2001

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidase activity of tyrosinase. For the inhibitory effect against DOPA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and water, respective The methylene chloride fractions of Angelica tenuissima, Nardostachys chinensis, Bombyx mop and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

• Journal of Applied Biological Chemistry
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• 제59권2호
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• pp.107-112
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• 2016

Pectolinarin was isolated from the ethyl acetate fraction of Cirsium setidens using open column chromatography and was analyzed using spectrometry. Pectolinarin content in Cirsium spp. was determined using HPLC/UV. Pectolinarin content in the aerial part of Cirsium spp. was higher than that in the root and pappus. Pectolinarin content was highest in the aerial part of C. chlorolepis (110.65mg/g extract). Consequently, the aerial part of C. chlorolepis has potential for use in new natural medicinal products, health supplements, and beverages.

• 한방안이비인후피부과학회지
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• 제18권3호
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• pp.37-43
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• 2005

This study was performed to determine the cytotoxic effect of methanol extract from Houttuynia Cordata and Glycine soja. The cell viability was determined by MTT method. Their cytotoxic activities against three cancer cell lines such as A549, MDA-MB-231 and SNU-C4 cell line were tested. Among them, The methanol extract of Houttuynia Cordata showed the strongest cytotoxic effect against SNU-C4 cells. These results suggest that the methanol extract of Houttuynia Cordata possessed a potential antitumorous agent. The free radical scavenging activity using DPPH method was the strongest of Houttuynia Cordata methanol extract and ethyl acetate fraction.

• 생약학회지
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• 제20권3호
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• pp.149-153
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• 1989

The presence of saponin, terpenoid, steroid, anthraquinone and flavonoid were screened with the usual test methods in forty five(sixty four parts) Korean plants belonging to Urticaceae, Celastraceae, Rhamnaceae, Sterculiceae and Rubiaceae. Plants were extracted with methanol and fractionated with hexane, chloroform, ethyl acetate and butanol. Each fraction was tested for the components. The result showed that in saponin test, 21 plants were positive and 11 plants were weak positive; in terpenoid and steroid test, 26 plants were strong positive; in anthraquinone test, 20 plants strong positive; in flavonoid test, 31 plants were positive and 9 plants were weak positive.

• Current Research on Agriculture and Life Sciences
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• 제30권2호
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• pp.110-114
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• 2012

In the present study, acetone extract and sub-fractions of Albizia amara stem bark were evaluated for their free radical potential. The results showed that the crude extract and all the fractions exhibited antioxidant and free radical scavenging activities under different in vitro assays. Among the different fractions, the ethyl acetate fraction exhibited higher DPPH and ABTS radical scavenging activities than the standard quercetin. A. amara stem bark might be valuable source of natural antioxidants that could be used for medicinal and food applications.

• Archives of Pharmacal Research
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• 제24권6호
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• pp.522-523
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• 2001

A new naphthoquinone, 5,8-dihydro-2,7-dimethyl-[1,4]naphthoquinone (1), which was named 5,8-dihysrochimaphilin, isolated from an ethyl acetate soluble fraction from the root of Pyrola japonica, together with chimaphilin (2). Compound 1 was transformed rapidly to 2 upon exposure to air by HPLC analysis. This fact supported that chimaphilin (2) may be an artifact from 1 .

• Natural Product Sciences
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• 제24권4호
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• pp.293-297
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• 2018

Sasa quelpaertensis Nakai leaves contain a mixture of polysaccharides, amino acids, and polyphenols, which mediate various biological activities. For efficient utilization of its leaf, we reported the preparation procedure for phytochemical-rich extract (PRE) using the leaf residue, which was by-product of hot water extraction. This study was undertaken to evaluate the effects of PRE and its major constituent, p-coumaric acid,on the growth of several human cancer cell lines (MKN-74, MKN-45, SNU-1, SNU-16, and HL-60). The ethyl acetate fraction of PRE and p-coumaric acid significantly inhibited the proliferation of MKN-74 and HL-60 cells, respectively, and induced cell apoptosis, down-regulated Bcl-2 and poly (ADP-ribose) polymerase levels, and up-regulated those of Bax and caspase-3. These results show the potential utility of S. quelpaertensis Nakai leaves in cancer prevention.