YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Screening of Inhibitors against Tyrosinase Activity from Natural Products

천연물로부터 티로시나제 활성 저해제의 검색

  • 최상숙 (중앙대학교 자연과학대학 화학과) ;
  • 노향순 (중앙대학교 자연과학대학 화학과) ;
  • 조성희 (중앙대학교 자연과학대학 화학과) ;
  • 공광훈 (중앙대학교 자연과학대학 화학과)
  • Published : 2001.10.01
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Abstract

Tyrosinase plays an important role in the process of melanin biosynthesis, and it is a biochemical target enzyme for skin-whitening agents and the remedy for disturbances in pigmentation. We have screened 25 natural plant extracts for inhibitory effects against DOPA oxidase activity of tyrosinase. For the inhibitory effect against DOPA oxidase activity of tyrosinase, the recombinant human tyrosinase and the purified mushroom tyrosinase were used. Each of the dried plants extracted with methanol, and then the extracts were subjected to sequential fractionations with methylene chloride, ethyl acetate, n-butanol and water, respective The methylene chloride fractions of Angelica tenuissima, Nardostachys chinensis, Bombyx mop and Saposhnikovia divaricata, and the n-butanol fraction of Bombyx mori notably inhibited the human tyrosinase activity as well as mushroom tyrosinase activity (more than 90% inhibition). This study suggests that the above extracts have a potential as whitening agents as single ingredients or in combination with other of the extracts.

Keywords

References

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