International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지

Inhibitory Effect of Continentalic Acid from Aralia continentalis on Streptococcus mutans Biofilm

  • Jeong, Seung-Il (Jeonju Biomaterials Institute, Wonkwang Univ.) ;
  • Lee, Sang-Bong (Department of Pedodontics, School of Dentistry, Wonkwang Univ.) ;
  • Moon, Hae-Dalma (Department of Oral Biochemistry, School of Dentistry, Wonkwang Univ.) ;
  • Ra, Ji-Young (Department of Pedodontics, School of Dentistry, Wonkwang Univ.) ;
  • Lee, Kwang-Hee (Department of Pedodontics, School of Dentistry, Wonkwang Univ.) ;
  • You, Yong-Ouk (Department of Oral Biochemistry, School of Dentistry, Wonkwang Univ.)
  • Received : 2010.10.21
  • Accepted : 2010.12.16
  • Published : 2010.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

In our present study, we investigated the effects of continentalic acid on Streptococcus mutans (S. mutans) biofilm. Methanol extract of Aralia continentalis (A. continentalis) was suspended in water and sequentially partitioned with CHCl3, ethyl acetate (EtOAc), and n-butanol (n-BuOH). The CHCl3 fraction showed the highest activity and an antibacterial compound against S. mutans was isolated from this preparation through various chromatography methods by bioassay guided fractionation. MS, $^1H-NMR$ and $^{13}C-NMR$ analysis showed that the active principle was continentalic acid which was confirmed to show significant inhibitory effects against S. mutans biofilm. These results may provide some scientific rationale for the traditional use these extracts for the treatment of dental diseases.

Keywords

References

  1. Chang KW, Koh KJ, Yoo YG. Antibacterial effects of Berberine to the mutans Streptococci. J Kor Acad Dent Health. 1997;21:537-544.
  2. Choi WC. Reasons for extraction of permanent teeth among primary dental consumers in metropolitan area of Korea. Department of preventive and Public Health Dentistry Graduate School, Seoul National Univ. Seoul, 1999.
  3. Chung DI, Ro JS, Chang KW. Antibacterial effect of some Flavonoids against Cariogenic bacteria. J Kor Acad Dent Health. 1996;20:189-202.
  4. Han BH, Han YN, Han KA, Park MH, Lee EO. Studies on the Anti-inflammatory Activity of Aralia continentalis (I) Characterization of Continentalic Acid and its Anti-inflammatory Activity. Arch Pharm Res. 1983;6:17-23. https://doi.org/10.1007/BF02855697
  5. Han BH, Park MH, Han YN, Manalo JB. Studies on the Antiinflammator Activity of Aralia continentalis (II) Isolation of Two Phenolic Acids from The Hydrolysate of Butanol Fraction. Arch Pharm Res. 1983;6:75-77. https://doi.org/10.1007/BF02855705
  6. Han BH, Woo ER, Park MH, Han YN. Studies on the Antiinflammatory Activity of Aralia continentalis (III). Arch Pharm Res. 1985;8:59-65. https://doi.org/10.1007/BF02912223
  7. Iniue M, Koga T, Sato S, Hamada S. Synthesis adherent insoluble glucan by the concerted action of the two glucosyltranferase components of Streptococcus mutans. FEBS Lett. 1982;143: 101-104. https://doi.org/10.1016/0014-5793(82)80282-2
  8. Jang SI, Lee HO, Kim KJ. Oral immunology, pp 216-231, Daehaksa. Iksan, 1998.
  9. Jeong SI, Han WS, Yun YH, Kim KJ. Continentalic acid from Aralia continentalis shows activity against methicillin-resistant Staphylococcus aureus. Phytother Res. 2006;20:511-514. https://doi.org/10.1002/ptr.1894
  10. Kim CM, Shin MK, Ahn DG, Lee KS. JungYakDaeSaJun, Illustrated Chinese Materia Medica: Jiangsu New Medical College, vol. 3, pp 1056-1064, Jungdam. Seoul, 1998.
  11. Kim CM, Shin MK, Ahn DG, Lee KS. JungYakDaeSaJun, Illustrated Chinese Materia Medica: Jiangsu New Medical College, vol. 7, pp 3969-3974, Jungdam. Seoul, 1998.
  12. Kim JB, Choi YJ. Public Oral Health, pp 68-84, Komunsa. Seoul, 1991.
  13. Kim JI. Clinical Herbology Course, pp 930-934, Daesung medical Publishers. Seoul, 2000.
  14. Kim JS, and Kang SS. Saponin from the aerial parts of Aralia continentalis. Nat Prod Sci. 1998;4:45-50.
  15. Koga T, Asakawa H, Okahashi N, Hamada S. Sucrose-dependent cell adherence and cariogenicity of serotype c Streptococcus mutans. J Gen Microbiol. 1986;10:2873-2883.
  16. Koga T, Hamada S, Murakawa S, Endo A. Effect of a glucosyltransferase inhibitor on glucan synthesis and cellular adherence of Streptococcus mutans. Infect Immun. 1982;38: 882-886.
  17. Lee HO, Lee KH, Park NK, Jeong SI, Baek SH, Han DM. Antibacterial effects of Sophora flabescens on Streptococcus mutans. J Kor Acad Dent Health. 2000;12:539-546.
  18. Lee SD (Original author, Huh, Joon, written in Chinese, 1613). DongeuiBogam, vol. 2, p773, Yeokang Publishers. Seoul, 1994.
  19. Lee YG, Choi YW, Kim YG, Kim KK. Antimicrobial effects of the Methanol extracts of Schlectendlaia mimifuehi matsumura on vibrio spp. J AGR Technol Dev Institute. 1999;3:201-204.
  20. Lim H, Jung HA, Choi JS, Kim YS, Kang SS, and Kim HP. Anti-inflammatory activity of the constituents ofthe roots of Aralia continentalis. Arch Pharm Res. 2009;32:1237-1243. https://doi.org/10.1007/s12272-009-1909-3
  21. Nakahara K, Kawabata S, Ono H, et al,. Inhibitory effect of oolong tea polyphenols on glycosyltransferases of mutans Streptococci. Appl Environ Microbiol. 1993;59:968-973.
  22. National Health Insurance Corporation(NHIC). 2004 National Health Insurance Statistical Yearbook, Benefits by Frequency pp 68-69, National Health Insurance Corporation. Seoul, 2005.
  23. National Health Insurance Corporation(NHIC). 2008 National Health Insurance Statistical Yearbook, Benefits by Frequency Table VI-3 p 586, National Health Insurance Corporation. Seoul, 2009.
  24. Otake S, Makimura M, Kuroki T, Nishihara Y, Hirasawa M. Anticaries effects of polyphenolic compounds from Japanese green tea. Caries Res. 1991;25:438-443. https://doi.org/10.1159/000261407
  25. Park CS, Kim SS, Kim SH, Shin YS, Lee DS, Kim KJ. Inhibitory effect of water extract of phellodendri cortex L. on the growth and the acid production of Streptococcus mutans JC-2. J Kor Acad Dent Health. 1995;19:439-446.
  26. Saeki Y, Kato T, and Naito Y. Inhibitory effects of funoran on the adherence and colonization of mutans Streptococci. Caries Res. 1996;30:119-125. https://doi.org/10.1159/000262147
  27. Sohn KC, Kim KH, Kim JB. Dental health of 12-year-old children in Pusan. J Kor Acad Dent Health. 1996;20:175-187.
  28. Translation Committee of DongeuiBogam (TCDB). DongeuiBogam (Original author, Huh, Joon, written in Chinese, 1613), p1632, Bubin Publishers. Seoul, 1999.
  29. Wenham DG, Davies RM, Cole JA. Insoluble glucan synthesis by mutansucrase as determinant of the cariogenicity of Streptococcus mutans. J Gen Microbiol. 1981;127:407-415.
  30. Yoo YK, Ro JS, Kim SS. The antibacterial effects of some Propolis constituents against S. mutans, Lactobaccilli and Actinomyces. J Kor Acad Dent Health. 1996;20:65-74.
  31. You-Ping Zhu. Chinese Materia Medica : Chemistry, Pharmacology and Applications, pp 450-454, Harwood academic publisher. Amsterdam, 1998.
  32. Yun-Choi HS, Kim JH, Lee JR. Screening of Potential Inhibitors of Platelet Aggregation from Plant Sources (II). Kor J Pharmacogn. 1986;17:19-22.