• Title/Summary/Keyword: ethyl-acetate fraction

Search Result 1,176, Processing Time 0.022 seconds

Antimutagenic and Antioxidative Effects of Methanol Extract of Pine Pollen (송화 메탄올 추출물의 항산화적 항돌연변이 효과)

  • 박정섭;안병용;최동성
    • The Korean Journal of Food And Nutrition
    • /
    • v.16 no.4
    • /
    • pp.303-309
    • /
    • 2003
  • This study was performed to investigate the antimutagenic and antioxidative activities of pine pollen with respect to the microbial mutation induced by various mutagens such as 1-NP, daunomycin, 2-NF, MNNG, NaN$_3$, 4NQO, 4-NOPD, AFB$_1$, Trp-P-1, 2-AF and oxidative mutagens such as t-BOOH, H$_2$O$_2$. Pine pollen, originally extracted with hexane, was reextracted with 70% methanol. The results obtained using the methanol extract, in terms of the antimutagenicity observed in relation to ten kinds of mutagens, showed that it exhibited 17.8, 82.2 and 80.9% inhibitory effects against daunomycin, AFB$_1$, and Trp-P-1, respectively, in Salmonella. typhimurium TA98 and a 72.3% inhibitory effect against AFB$_1$in S. tyPhimurium TA100. In terms of the antimutagenicity exhibited in relation to t-BOOH, a 72.3% inhibitory effect was observed, but no antimutagenicity was observed in relation to the other mutagens and strains. The methanol extract was further fractionated by chloroform, ethyl acetate, n-butanol. In S. typhimurium TA98, the chloroform(150 $\mu\textrm{g}$/plate) fraction showed strong antimutagenic effects of 55.6%, 93.7% and 93.5%, while the ethyl acetate(100 $\mu\textrm{g}$/plate) fraction showed 11.4%, 74.3% and 85.2% in relation to the mutagenicity induced by daunomycin, AFB$_1$and Trp-P-1, respectively. In S. typhimurium TA100, the chloroform and ethyl acetate fractions showed antimutagenic effects of 95.1% and 62.5%, respectively, on the mutagenicity induced by AFB$_1$. In S. typhimurium TA102, the chloroform fraction showed an antimutagenic effect of 93.6% on the mutagenicity induced by t-BOOH.

Detection of Endolichenic Fungi Producing Antifungal Compound (항진균성 물질을 생산하는 지의류 내생 곰팡이의 탐색)

  • Kim, Eun-Sung;Choi, Kap-Seong;Choi, Sang-Ki
    • Microbiology and Biotechnology Letters
    • /
    • v.40 no.1
    • /
    • pp.23-29
    • /
    • 2012
  • To isolate a novel antifungal compound, we obtained 100 kinds of endolichenic fungi from Korean Lichen & Allied Bioresources Center and examined their antifungal capability. Three fungi Usnea rigidula (2326), Parmotrema pseudotinctorum (2202) and Myelochroa sp. (2292) showed high antifungal activity against Candida albicans when they grew in both liquid and solid media. We extracted the culture supernatants of these three fungi with chloroform and then with ethyl acetate. Chloroform fraction exhibited the highest antifungal activities when those fractions were examined for the growth inhibition of Candida albicans with disc diffusion method. The chloroform faction was on further analysis with $C_{18}$ column chromatography to see whether the inhibitors are already known or not. Two peak fractions were collected from 4-day culture extract for Usnea rigidula and from 6-day culture extract for Parmotrema pseudotinctorum on the HPLC. A peak fraction from chloroform extracts of 4-day culture filtrate of Parmotrema pseudotinctorum showed higher antifungal activities against C. albicans and C. glabrata than another peak fraction. It appears that the antifungal materials are relatively nonpolar as usnic acid often found in lichenic fungi.

Protective Effects of Cellular Membrane and Component Analysis of Polygonum aviculare Extracts (마디풀 추출물의 세포 보호 효과 및 주성분 분석)

  • Park, Soo Nam;Kim, Min-Ji;Kim, Su Ji
    • Microbiology and Biotechnology Letters
    • /
    • v.42 no.1
    • /
    • pp.51-57
    • /
    • 2014
  • In this study, the antioxidative effects and component analysis of Polygonum aviculare (P. aviculare) extracts were investigated. The ethyl acetate and the aglycone fraction from P. aviculare extracts were more active than (+)-${\alpha}$-tocopherol and $\small{L}$-ascorbic acid, which are known as strong antioxidants for their antioxidative activity by the DPPH method and chemiluminescence assay. The cellular protective effects of fractions of P. aviculare on the rose-bengal sensitized photohemolysis of human erythrocytes, increased in a concentration dependent manner ($1-10{\mu}l$). In particular, the ethyl acetate fraction at a concentration of $10{\mu}l$ showed the most prominent protective effect among all the extracts (${\tau}_{50}$, 314.70 min). TLC and HPLC chromatogram of the ethyl acetate fraction of P. aviculare extracts revealed 3 main bands (PA8, PA5, PA6) and peaks (peak 1, peak 2, peak 3), which were identified as myricetin-3-O-rhamnoside (myricitrin, PA8, peak 1), quercetin-3-${\alpha}$-rhamnoside (quercitrin, PA6, peak 3) by LC/ESI-MS/MS and $^1H$-NMR respectively. These results indicate that fractions from P. aviculare could be applicable to new functional cosmetics as antioxidants.

Antifungal Activity of the Quercus Mongolica Extracts Against Botrytis cinerea (신갈나무로부터 유래된 추출물의 Botrytis cinerea 균주에 대한 항균활성)

  • Yeo, Hee Dong;Lee, Hyung Chul;Lim, Bu Kug;Kim, Hee Kyu;Choi, Myung Suk;Yang, Jae-Kyung
    • Journal of the Korean Wood Science and Technology
    • /
    • v.36 no.1
    • /
    • pp.88-101
    • /
    • 2008
  • The aim of this study was to attempt the efficacy of antifungal activity of the wood extracts against Botrytis grey mold. Wood chip derived from Quercus mongolica was obtain from steam explosion process and extracted by hot water and methanol and ethanol. The conidial germination was maximum growth with the application temperature for 20 and $25^{\circ}C$. In pH test, we observed the maximum growth in pH 5.0 and 7.0. Antifungal activity was the best in the hot water extractives against Botrytis cinerea. The separation of the antifungal substances was performed using a silica-gel column (n-hexane : chloroform : ethyl acetate : formic acid = 12 : 17 : 8 : 0.2, v/v/v/v), TLC and UV-Spectrophotometer, and isolated 6 fraction group. The result of antifungal activity in 6 fraction group, fraction group I and fraction group II were the highest antifungal activity against grey mold with the present study. Three peaks in fraction group I and II were detected by HPLC and this compounds were suppose to effective of antifungal activity.

Bio-antimutagenic effects of water extract from Rehmannia glutinosa Liboschitz in SOS Chromotest (SOS Chromotest에서 숙지황 물 추출물의 세포내 항돌연변이 효과)

  • Ahn, Byung-Yong;Lee, Kap-Sang;Maeng, Il-Kyung;Song, Geun-Seoub;Choi, Dong-Seong
    • Korean Journal of Food Science and Technology
    • /
    • v.30 no.2
    • /
    • pp.439-445
    • /
    • 1998
  • The antimutagenic activity of the water extract of Rehmannia glutinosa Liboschitz (RG) on the mutagenicity induced by 4-nitroquinoline 1-oxide (4-NQO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), $aflatoxin\;B_1\;(AFB_1)$ and benzo(a)pyrene [B(a)P] were studied using the SOS Chromotest with Escherichia coli PQ37. The water extract of RG was separated into methanol soluble and methanol insoluble parts. The methanol soluble part exhibited higher inhibition effects than the methanol insoluble part against the mutagenic activities of five mutagens. Step-wise fractionation of methanol soluble part was done using methanol, ethyl acetate and water. Among these fractions, water fraction had the strongest inhibitory effects against the mutagenenicity of five model mutagens, showing $4.5{\sim}29.5%$ inhibition, but the $AFB_1$ mutagenic potency was increased slightly by ethyl acetate fraction. The water fraction was further partitioned by sephadex LH-20 column chromtography, and 9 subfractions were obtained. The fraction III showed the strongest inhibitory effects with dose response against the mutagenic activities induced by all the tested chemical mutagens. The inhibition rates of fraction III at concentration of $400\;{\mu}g/assay$ were 29%, 35%, 38%, 25% and 24% against 4-NQO, MNNG, MMC, AFBl and B(a)P, respectively. The fraction III also exhibited a strong bio-an-timutagenicity against 4-NQO and $AFB_1$ by showing more than 40% inhibition.

  • PDF

Cytoprotective Effect of Organic Solvents Extracts of Sophorae Radix in H9c2 Cells (심근세포에서 고삼 유기용매 추출물의 항독성 효과)

  • Kwon Kang Beom;Kim Eun Kyung;Lim Yang Eui;Song Yung Sun;Park Jong Ha;Moon Hyung Cheal;Ryu Do Gon
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.18 no.3
    • /
    • pp.754-758
    • /
    • 2004
  • To test the cytoprotective effect of sophorae radix (SR) against hydrogen peroxide (H₂O₂)-induced cytotoxicity, we investigated the cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in the presence of methylene chloride, n-butanol, ethyl acetate and water soluble fraction of SR water extracts in H9c2 cells. These results were obtained as followed; H₂O₂ decreased the cell viability of H9c2 cells in a dose dependent manner. Cells pretreated with SR water extracts were protected the H₂O₂-induced decrease of viability in H9c2 cells. Among organic solvents fractions of SR water extracts, ethyl acetate soluble fractions of SR protected the decrease of viability induced by H₂O₂ in H9c2 cells. These results suggest that ethyl acetate soluble fractions of SR water extracts is effective in the prevention of H₂O₂-induced cytotoxicity.

Studies on the Cytotoxicity of the Ethyl Acetate Soluble Sophora flavescens Ait. Extract against L1210 and $P388D_1$ Cells (III) (L1210 및 $P388D_1$ 세포에 대한 고삼 에틸 아세테이트 추출물의 세포독성에 관한 연구 (III))

  • Ryu, Hong-Sun;Shin, Min-Kyo;Yang, Eun-Yeong;Cho, Hoon;Chai, Kyu-Yun;Kang, Kil-Ung;Baek, Seung-Hwa
    • Korean Journal of Pharmacognosy
    • /
    • v.31 no.1
    • /
    • pp.51-56
    • /
    • 2000
  • This study was carried out to evaluate cytotoxic effects of the roots of Sophora flavescens Ait. extracts on murine leukemia tumor cells lines $(P388D_1\;and\;L1210)$. Disruptions in cell organelles were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The comparison of $IC_{50}$ values of the ethyl acetate of Sophora flavescens Ait. extract in leukemia cell lines showed that their susceptibility to these extracts decreased in the following order : Adriamycin>Fr.4>Fr.5>Fr.3>Fr.1>Fr.2 by the MTT assay. These results suggest that the fraction 4 of the ethyl acetate soluble extract of Sophora flavescens Ait. may be a valuable choice for the studies on the treatment of murine leukemia cell lines.

  • PDF

Flavonoids from the Leaves of Ailanthus altissima Swingle and their Antioxidant Activity

  • Lee, Min-Kyung;Kim, Su-Yeon;Park, Ji-Hae;Lee, Do-Gyeong;Lee, Dae-Young;Kim, Geum-Soog;Kim, Yong-Bum;Han, Dae-Seok;Lee, Chang-Ho;Baek, Nam-In
    • Journal of Applied Biological Chemistry
    • /
    • v.56 no.4
    • /
    • pp.213-217
    • /
    • 2013
  • Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.

Seasonal variation of antimicrobial and antioxidative activity in Artemisia princeps var. orientalis

  • Yun, Kyeong-Won;Park, Seongkyu;Jung, Hyung-Jin
    • Proceedings of the Plant Resources Society of Korea Conference
    • /
    • 2003.10b
    • /
    • pp.44-44
    • /
    • 2003
  • Leaves from natural populations of Artemisia princeps var. orientalis in Suncheon of South Korea were examined for antimicrobial and antioxidative activities monthly (April through October). The antimicrobial activity of the ethyl acetate and water fractions of crude methanol extract from the plant collected monthly against three gram-positive, two gram-negative and one lactic acid bacteria were studied. The ethyl acetate and water fractions of crude methanol extract from A. princeps var. orientalis collected in August and September had the highest antimicrobial activity. Minimum inhibitory concentrations (MIC) for each strain by ethyl acetate fraction of methanol extract from A. princeps var. orientalis was different depending on strains and sampling months. MIC for each strain was the highest in the sample of April, followed by October. In specific, MIC for Bacillus cereus was around 0.25mg/disc in the sample of April. The MIC for Staphyllococcus aureus was 0.01mg/disc in the sample of July, August, and September. It was the lowest value among tested strains and samples. And the antimicrobial activity for Lactobacillus plantarum was not found at any concentrations and sampling months. The in vitro antioxidative activities of methanol extract from Artemisia princeps var. orientalis collected monthly were determined by the scavenging of DPPH radical and inhibition of xanthine oxidase activity. The DPPH scavenging activity of the extracts from the plant sampled in May, June and July were higher than any other months. The xanthine oxidase activity of the extract of A. princeps var. orientalis collected in April and May showed the greatest activity, it is different with antimicrobial activity.

  • PDF

Identification and Characterization of Fibrinolytic Compound from Cornus officinalis S. et Z (산수유(Cornus officinalis)로부터 혈전용해물질의 확인 및 특성 연구)

  • Kim, Jun-Ho
    • Korean Journal of Plant Resources
    • /
    • v.33 no.4
    • /
    • pp.237-244
    • /
    • 2020
  • The objective of this study was to identify and characterize fibrinolytic compound from Cornus officinalis. Cornus officinalis. Hot water extract was fractionated into hexane, chloroform, ethyl acetate, butanol, and water fractions. Assays for fibrinolytic activity indicated that only the ethyl acetate fraction had significant efficacy at 1.36 plasmin units/mL. Isolation of fibrinolytic compound was carried out on Amberlite IRA-400, Sephadex LH-20 and active charcoal column chromatography. HPLC analysis of the purified fibrinolytic compound showed retention time (RT) same as authentic malic acid. LC / MS / MS in negative mode showed the same peak at m/z 133, confirming that the purified compound was malic acid with a molecular weight 134 Da. The compound showed fibrinolytic activity of 0.69 plasmin units/mL, 14.62% of thrombin inhibitory activity, 6.42% of antioxidative activity, and 17.28% of α-glucosidase inhibitory activity. The purified compound hydrolyzed γ subunits of human fibrinogen. In conclusion, malic acid isolated from Cornus officinalis might have potential to be developed as ingredient for biofunctional foods to prevent cardiovascular diseases.