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http://dx.doi.org/10.4014/kjmb.1201.01001

Detection of Endolichenic Fungi Producing Antifungal Compound  

Kim, Eun-Sung (Department of Biological Sciences at Sunchon National University)
Choi, Kap-Seong (Department of Food Science and Technology at Sunchon National University)
Choi, Sang-Ki (Department of Biological Sciences at Sunchon National University)
Publication Information
Microbiology and Biotechnology Letters / v.40, no.1, 2012 , pp. 23-29 More about this Journal
Abstract
To isolate a novel antifungal compound, we obtained 100 kinds of endolichenic fungi from Korean Lichen & Allied Bioresources Center and examined their antifungal capability. Three fungi Usnea rigidula (2326), Parmotrema pseudotinctorum (2202) and Myelochroa sp. (2292) showed high antifungal activity against Candida albicans when they grew in both liquid and solid media. We extracted the culture supernatants of these three fungi with chloroform and then with ethyl acetate. Chloroform fraction exhibited the highest antifungal activities when those fractions were examined for the growth inhibition of Candida albicans with disc diffusion method. The chloroform faction was on further analysis with $C_{18}$ column chromatography to see whether the inhibitors are already known or not. Two peak fractions were collected from 4-day culture extract for Usnea rigidula and from 6-day culture extract for Parmotrema pseudotinctorum on the HPLC. A peak fraction from chloroform extracts of 4-day culture filtrate of Parmotrema pseudotinctorum showed higher antifungal activities against C. albicans and C. glabrata than another peak fraction. It appears that the antifungal materials are relatively nonpolar as usnic acid often found in lichenic fungi.
Keywords
Antifungal; Endolichenic fungi; Usnea rigidula; Parmotrema pseudotinctorum; Myelochroa sp.;
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1 Michael L., O. Margret, B. Klaus, P. Thies, and M. Reinhard. 1995. In vitro activities of the lichen secondary metabolites vulpinic acid, (+)-usnic acid, and (-)-usnic acid against aerobic and anaerobic microorganisms. Antimicrob. Agents Chemother. 39: 2541-2543.
2 Rankovie B., D. Rankovic, and D. Maric. 2010. Antioxidant and antimicrobial activity of some lichen species. Microbiology. 79: 809-815.
3 Hur J. S., G. S. Han, J. W. Kim, and Y. W. Lee. 1999. Isolation of antagonistic fungi associated with the lichens distributed in southern parts of Korea. J. Plant Pathol. 15: 280-286.
4 John A. G. R., M. M. Steven, M. Kim, and Y. J. W. Jason. 2006. Determination of usnic acid in lichen toxic to elk by liquid chromatography with ultraviolet and tandem mass spectrometry detection. J. Agric. Food Chemother. 54: 2484- 2490.
5 Kumar K. C., and K. Muller. 1999. Lichen metabolites. 2. Antiproliferative and cytotoxic activity of gyrophoric, usnic, and diffractaic acid on human keratinocyte growth. J. Nat. Prod. 62: 821-823.
6 Lawrey, J. D. 1989. Lichen secondary compounds: evidence for a correspondence between antiherbivore and antimicrobial function. J. Bryol.. 92: 326-328.