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Cytoprotective Effect of Organic Solvents Extracts of Sophorae Radix in H9c2 Cells  

Kwon Kang Beom (Department of Physiology, Wonkwang University)
Kim Eun Kyung (Department of Physiology, Wonkwang University)
Lim Yang Eui (Department of Oriental Rehabilitation Medicine, Wonkwang University)
Song Yung Sun (Department of Oriental Rehabilitation Medicine, Wonkwang University)
Park Jong Ha (Department of Oriental Rehabilitation Medicine, Wonkwang University)
Moon Hyung Cheal (Department of Acupuncture & Moxibustion, School of Oriental Medicine, Wonkwang University)
Ryu Do Gon (Department of Physiology, Wonkwang University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.18, no.3, 2004 , pp. 754-758 More about this Journal
Abstract
To test the cytoprotective effect of sophorae radix (SR) against hydrogen peroxide (H₂O₂)-induced cytotoxicity, we investigated the cell viability using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in the presence of methylene chloride, n-butanol, ethyl acetate and water soluble fraction of SR water extracts in H9c2 cells. These results were obtained as followed; H₂O₂ decreased the cell viability of H9c2 cells in a dose dependent manner. Cells pretreated with SR water extracts were protected the H₂O₂-induced decrease of viability in H9c2 cells. Among organic solvents fractions of SR water extracts, ethyl acetate soluble fractions of SR protected the decrease of viability induced by H₂O₂ in H9c2 cells. These results suggest that ethyl acetate soluble fractions of SR water extracts is effective in the prevention of H₂O₂-induced cytotoxicity.
Keywords
Sophorae Radix; Hydrogen Peroxide; H9c2 Cells; 3-(4,5-dimethylthiazol-2-yl)-2; 5-diphenyl-2H-tetrazolium bromide;
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