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http://dx.doi.org/10.5658/WOOD.2008.36.1.088

Antifungal Activity of the Quercus Mongolica Extracts Against Botrytis cinerea  

Yeo, Hee Dong (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University)
Lee, Hyung Chul (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University)
Lim, Bu Kug (Yulim Hightech. co)
Kim, Hee Kyu (Department of Applied Biology & Environmental Sciences, Gyeongsang National University)
Choi, Myung Suk (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University)
Yang, Jae-Kyung (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University)
Publication Information
Journal of the Korean Wood Science and Technology / v.36, no.1, 2008 , pp. 88-101 More about this Journal
Abstract
The aim of this study was to attempt the efficacy of antifungal activity of the wood extracts against Botrytis grey mold. Wood chip derived from Quercus mongolica was obtain from steam explosion process and extracted by hot water and methanol and ethanol. The conidial germination was maximum growth with the application temperature for 20 and $25^{\circ}C$. In pH test, we observed the maximum growth in pH 5.0 and 7.0. Antifungal activity was the best in the hot water extractives against Botrytis cinerea. The separation of the antifungal substances was performed using a silica-gel column (n-hexane : chloroform : ethyl acetate : formic acid = 12 : 17 : 8 : 0.2, v/v/v/v), TLC and UV-Spectrophotometer, and isolated 6 fraction group. The result of antifungal activity in 6 fraction group, fraction group I and fraction group II were the highest antifungal activity against grey mold with the present study. Three peaks in fraction group I and II were detected by HPLC and this compounds were suppose to effective of antifungal activity.
Keywords
antifungal activity; wood extracts; Quercus mongolica; Botrytis cinerea;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Lee, H. J., S. S. Lee, and D. H. Choi. 2003. Studies on biological activity of wood extractives-Antimicrobial and antioxidative activities of extractive from the heart wood of Prunus sargentii. Kor. Mokchae Konghak. 31(4): 16-23.
2 Talma, K., Y. Elad, and H. Yunis. 1989. Resistance to diethofencarb (NPC) in benomyl-resistant field isolates of Botrytis cinerea. Plant Pathology. 38(1): 86.   DOI
3 Sansone, G., I. Rezza, V. Calvente, D. Benuzzi, and M. I. Sanz de Tosetti. 2005. Control of Botrytis cinerea strains resistant to iprodione in apple with rhodotorulic acid and yeasts. Postharvest Biology and Technology. 35: 245-251.   DOI   ScienceOn
4 Daulagala, E., G. W. Tsomlexoglou, L. A. Gooday, G. B. Seddon, and E. J. Allan. 2003. Molecular detection and b-glucuronidase expression of gusmarked Bacillus subtilis L-form bacteria in developing Chinese cabbage seedlings. Journal of Applied Microbiology. 95(2): 218-224.   DOI   ScienceOn
5 Kang, J. S., O. H. Jhee, J. W. Hong, A. S. Om, M. H. Lee, W. S. Lee, L. M. Shaw, and J. W. Lee. 2005. Direct determination of verapamil in rat plasma by coupled column microbore-HPLC method. Journal of Pharmaceutical and Biomedical Analysis. 37: 405-410.   DOI   ScienceOn
6 Nikos, G. T. and C. D. Economakis. 2007. Antifungal activity of lemongrass (Cympopogon citratus L.) essential oil against key postharvest pathogens. Innovative Food Science and Emerging Technologies. 8: 253-258.   DOI   ScienceOn
7 Nelson, M. E. and M. L. Powelson. 1988. Biological control of gray mold of snap beans by Trichoderma hamatum. Plant Dis. 72: 727-729.   DOI
8 Beever, R. E. and L. Parkes. 1993. Mating behaviour and genetics of fungicide resistance of Botrytis cinerea in New Zealand. New Zealand J. Crop & Horti. Sci. 21: 303-310.   DOI   ScienceOn
9 Byun, H. J. and S. J. Choi. 2003. Suppression of Post-harvest grey mold rot incidence in strawberry by field application of hydrogen peroxide. J. Kor. Soc. Hort. Sci. 44(6): 859-862.   과학기술학회마을
10 Elad, Y., N. E. Malathrakist, and A. J. Dik. 1996. Biological control of Botrytis-incited diseases and powdery mildews in greenhouse crops. Crop Protection. 1(3): 229-240.
11 Faretra, F. and S. Pollastro. 1993. Genetics of sexual compatibility and resistance to benzimidazole and dicarboximide fungicides in isolates of Botryotinia fuckeliana from nine countries. Plant Pathology. 42: 48-52.   DOI
12 Hmamouchi, M., C. Bouchraa, M. Achourib, and L. M. Idrissi Hassanic. 2003. Chemical composition and antifungal activity of essential oils of seven Moroccan Labiatae against Botrytis cinerea Pers: Fr. Journal of thnopharmacology. 89(1): 165-169.   DOI   ScienceOn
13 Kim, J. Y., E. A. Bae, M. J. Han, and D. H. Kim. 1999. Inhibitiory activity of Bacillus licheniformis AJ on the growth of diarrheal pathogens. J. Apple. Pharm. 7: 385-389.
14 Kim, M. Y., Y. G. Kim, T. H. Kim, J. S. Jo, and J. K. Yang. 2000. Antimicrobial activity and antioxidative activity in the extractives of Quercus dentata Thunberg. Mokchae Konghak. 28(3): 42-51.
15 Lee, B. W. and D. H. Shin. 1991. Screening of Natural Antimicrobial Plant Extract on food Spoilage Microorganisms. Korea J. Food Sci. Technol. 23(2): 352-379.
16 Park, S. M., H. J. Jung, and S. H. Yeo. 2005. Antigungal activity of extract from Xanthium strumarium L. against plant pathogenous fungi. J. Life. Sci. 15: 692-695.   과학기술학회마을   DOI   ScienceOn
17 Koo, H. M., S. j. An, and H. C. Shim. 2006. Effect of mepanipyrim on the resistant isolates of gray mold fungus, Botrytis cinerea to the fungicides of benzimidazole and dicarboximide. J. Korean Soc. Appl. Biol. Chem. 49(4): 259-265.   과학기술학회마을
18 Min, K. H. 1998. Antifungal activity of the extracts of Zanthoxylum schinfolium sieb. et zucc. against dermatophytes. Mokchae Konghak. 26(4): 78-85.
19 Zhentian, L., j. Jervis, and R. F. Helm. 1999. C-Glycosidic ellagitannins from white oak heartwood and callus tissues. Phytochemistry. 51: 751-756.   DOI   ScienceOn
20 Park, Y. K., H. J. Lee, S. S. Lee, D. H. Choi, W. H. Yeo, and J. S. Oh. 2001. Studies on biological activity of wood extractives - Antifungal activity of isoflavonoids from Sopbora japonica. Mokchae Konghak. 29(4): 89-96.
21 Rosslenbroich, H. J. and S. Dietrich. 2000. Botrytis cinerea-history of chemical control and novel fungicides for its management. Crop Protection. 19(8-10): 557-561.   DOI   ScienceOn