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http://dx.doi.org/10.4014/kjmb.1311.11010

Protective Effects of Cellular Membrane and Component Analysis of Polygonum aviculare Extracts  

Park, Soo Nam (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology)
Kim, Min-Ji (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology)
Kim, Su Ji (Department of Fine Chemistry, Nanobiocosmetic laboratory, and Cosmetic R&D Center, Seoul National University of Science and Technology)
Publication Information
Microbiology and Biotechnology Letters / v.42, no.1, 2014 , pp. 51-57 More about this Journal
Abstract
In this study, the antioxidative effects and component analysis of Polygonum aviculare (P. aviculare) extracts were investigated. The ethyl acetate and the aglycone fraction from P. aviculare extracts were more active than (+)-${\alpha}$-tocopherol and $\small{L}$-ascorbic acid, which are known as strong antioxidants for their antioxidative activity by the DPPH method and chemiluminescence assay. The cellular protective effects of fractions of P. aviculare on the rose-bengal sensitized photohemolysis of human erythrocytes, increased in a concentration dependent manner ($1-10{\mu}l$). In particular, the ethyl acetate fraction at a concentration of $10{\mu}l$ showed the most prominent protective effect among all the extracts (${\tau}_{50}$, 314.70 min). TLC and HPLC chromatogram of the ethyl acetate fraction of P. aviculare extracts revealed 3 main bands (PA8, PA5, PA6) and peaks (peak 1, peak 2, peak 3), which were identified as myricetin-3-O-rhamnoside (myricitrin, PA8, peak 1), quercetin-3-${\alpha}$-rhamnoside (quercitrin, PA6, peak 3) by LC/ESI-MS/MS and $^1H$-NMR respectively. These results indicate that fractions from P. aviculare could be applicable to new functional cosmetics as antioxidants.
Keywords
Polygonum aviculare; antioxidative activity; cellular protective effect; components analysis; myricitrin;
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Times Cited By KSCI : 3  (Citation Analysis)
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