• 대한약침학회지
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• 제16권3호
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• pp.23-29
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• 2013

Objectives: Dyslipidemia in diabetes mellitus is a significant risk factor for the development of cardiovascular complications. The aim of this study was to evaluate the effect of the ethyl-acetate fraction of an ethanolic extract from Streospermum suaveolens on lipid metabolism in streptozotocin (STZ)-induced diabetic rats. Methods: Diabetes was induced by intraperitonial injection of STZ (50 mg/kg). Diabetic rats were treated with an ethyl-acetate fraction orally at doses of 200 and 400 mg/kg daily for 14 days. On the $15^{th}$ day, serum lipid profiles, such as total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), were estimated in experimental rats. The atherogenic (AI) and the coronary risk (CRI) indices were also evaluated. Results: The ethyl-acetate fraction at doses of 200 and 400 mg/kg significantly (P < 0.001) and dose-dependently reduced serum cholesterol, triglycerides and LDL, but increased HDL towards near normal levels as compared to diabetic control rats. The fraction also significantly (P < 0.001) lowered the atherogenic index (AI) and coronary risk index (CAI) in a dose-dependent manner. Conclusion: The present study demonstrated that the ethyl-acetate fraction of Stereospermum suaveolens exhibits a potent antihyperlipidemic activity in hyperglycemic rats and suggests that the plant may have therapeutic value in treating the diabetic complication of hyperlipidemia.

• 한국식품영양과학회지
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• 제35권2호
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• pp.182-186
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• 2006

본 연구에서는 연잎 추출분획물의 항산화 활성을 비교, 검토하고자 하였다. $70\%$ ethanol로 추출한 추출수율은 $8.16\%$였으며 이를 hexane, chloroform, ethyl acetate, butanol, water로 순차 분획한 수율은 butanol층이 $36.12\%$로 가장 높은 수율을 보였으나 대체적으로 극성이 높을수록 높은 분획수율을 나타내었다. 분회물간 총 페놀함량 측정결과 ethyl acetate 분획물의 총 페놀함량이 가장 높게 나왔으며 전자공여능 측정결과 또한 ethyl acetate 분획물에서 가장 우수한 결과를 나타내었다. Ethyl acetate 분획물의 전자공여능은 BHA, BHT와 비교한 결과 BHA와 유사한 결과를 나타내었으며 BHT보다 우수한 값을 나타내었다. 항산화 활성이 높게 나타난 ethyl acetate 분획물을 취하여 TBA가에 의한 지질과산화를 측정한 결과 BHA가 가장 우수한 활성을 보여 주었으며 ethyl acetate 분획물은 BHT보다도 활성이 떨어졌으나 이는 ethyl acetate 분획물이 단일물질로 분리정제가 이루어지지 않은 것으로 malonaldehyde 이외 다른 성분이 BHA와 반응하여 발색한 것으로 추측되어 졌다. AV와 POV 측정 결과 BHA가 가장 우수한 활성을 보여주었으나 ethyl acetate 분획물과 유의적인 차이는 없었으며 BHT에 비해서는 우수한 활성을 나타내었다.

• 생약학회지
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• 제44권4호
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• pp.379-383
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• 2013

Portulaca oleracea L. is known to have many biological benefits such as anti-oxidant, anti-inflammatory, anti-allergic and anti-tumor. The objective of this study is to explore the neuroprotective effect of P. oleracea L. against glutamate-induced oxidative stress in mouse hippocampal HT22 cells. P. oleracea L. 70% ethanol extract and solvent fractions have the potent neroprotective effects on glutamate-induced nerotoxicity by induced the expression of heme oxygenase (HO)-1 in HT22 cells. Especially, ethyl acetate fraction showed higher protective effect. In HT22 cell, P. oleracea L. treatment with ERK inhibitor (PD98059) and c-JUN N-terminal kinase (JNK) inhibitor (SP600125) reduced P. oleracea L. ethyl acetate fraction induced HO-1 expression and P. oleracea L. ethyl acetate fraction also increased ERK and JNK phosphorylation. Furthermore, we found that treatment of P. oleracea L. caused the nuclear accumulation of Nrf2. In conclusion, the ethyl acetate fraction of 70% ethanol extract of P. oleracea L. significantly protect glutamate-induced oxidative damage by induction of HO-1 via Nrf2, ERK and JNK pathway in mouse hippocampal HT22. Taken together these finding suggest that P. oleracea L. ethyl acetate fraction is good source for taking active compounds and may be a potential therapeutic agent for brain disorder that induced by oxidative stress and neuronal damage.

• 한국자원식물학회지
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• 제25권3호
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• pp.299-307
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• 2012

This work aimed to elucidate the anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino with a cellular system of LPS-stimulated RAW 264.7 and THP-1 cells. Some key pro-inflammatory cytokines and mediators including NO, iNOS, $PGE_2$, COX-2, TNF-${\alpha}$, NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65 were studied by sandwich ELISA and western blot analysis. Ethyl acetate fraction could significantly inhibit the production of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 in LPS-stimulated cell than that of single LPS-stimulated. And ethyl acetate fraction suppresses the activation of NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65. All the results showed that ethyl acetate fraction had a good anti-inflammatory effect on LPS-stimulated RAW264.7 and THP-1 cells. Taken together, the anti-inflammatory actions of ethyl acetate fraction from Cnidium officinale Makino might be due to the down-regulation of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 via the suppression of NF-${\kappa}B$ activation.

• 한국식품저장유통학회지
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• 제10권4호
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• pp.540-546
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• 2003

고추잎을 기능성식품의 재료로 이용하기 위한 방안을 모색하기 위하여 각 용매 분획물을 이용하여 생리활성 즉, 전자공여효과, 환원력, 아질산염 소거효과, 항균활성 및 tyrosinase 저해활성에 대하여 조사하였다 추출수율을 조사한 결과 물 분획층에서 16.9%로 가장 높은 추출수율을 나타낸 반면 에틸아세테이트 분획층 0.6%로 가장 낮은 추출수율을 나타내었다. 전자공여효과는 전반적으로 농도가 상승함에 따라 증가하였으며, 환원력도 분획물의 농도가 높아짐에 따라 점차적으로 증가하는 것을 볼 수 있었는데, 특히 ethyl acetate 분획물 0∼600 $\mu\textrm{g}$을 첨가하였을 때는 환원력의 증가가 크게 나타나지 않았으나, 900 $\mu\textrm{g}$이상 첨가구에서는 추출물을 첨가하지 않은 대조구와 비교하여 환원력이 매우 증가하는 것으로 나타났다. 고추잎 분획물을 농도별로 첨가하여 아질산염 소거효과를 측정한 결과 pH가 증가함에 따라 아질산염 소거효과가 감소하는 경향을 나타내었으며, ethyl acetate fraction에서 가장 높은 소거효과를 나타내어 500 $\mu\textrm{g}$을 첨가하였을 때 90%이상의 아질산염 소거효과를 나타내었으며, 농도가 증가함에 따라 아질산염 소거효과도 점차적으로 증가하는 경향을 나타내었다. 고추잎 각 분획물의 항균활성은 ethyl acetate 및 butanol 분획물에서 높게 나타났으며, 각 균주에 대한 clear zone의 직경을 확인한 결과 gram 양성균인 Bacillus cereus에서 20 mm로 항균활성이 가장 높게 나타났고, Staphylococcus aureus 18 mm, Streptecoccus mutans 17 mm순으로 주로 gram 양성균에서 높은 활성을 나타내었다. Tyrosinase 저해활성은 에틸 아세테이트 분획물에서 가장 높은 저해 활성을 나타내었다.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.264-270
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• 2021

본 연구는 복분자 에탄올 추출물과 용매 분획물의 총 폴리페놀 함량 및 총 플라보노이드 함량과 라디칼 소거능과 환원력을 이용한 항산화 활성 측정하였다. 총 폴리페놀 함량은 70% 에탄올 추출물 238.46 mg GAE/g 으로 나타났으며, ethyl acetate 분획물 413.59 mg GAE/g 으로 다른 분획물보다 높은 함량을 나타내었다(p<0.05). 또한 총 플라보노이드 함량도 ethyl acetate 분획물에서 52.83 mg QE/g으로 높은 함량을 나타내었다(p<0.05). DPPH 라디칼 소거능과 ABTS 라디칼 소거능의 경우 ethyl acetate 분획물의 RC₅₀값이 각각 3.95와 6.25 ㎍/mL 으로 대조군인 ascorbic acid와 유사한 소거능을 나타내었다(p<0.05). FRAP에 의한 환원력 측정 결과에서도 ethyl acetate 분획물에서 높은 활성을 나타내었다(p<0.05). 이상의 결과 복분자 70% 에탄올 추출물과 용매 분획물 중 ethyl acetate 분획물이 다량의 폴리페놀과 플라보노이드를 함유하고 있으며, 항산화 효과가 높게 나타났다. 따라서 본 연구의 결과는 복분자를 기능성 신소재 개발 원료로 활용하는데 기초 자료가 될 것으로 기대된다.

• 한국식품영양과학회지
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• 제41권9호
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• pp.1220-1225
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• 2012

본 연구는 곤달비(Ligularia stenocephala)의 생리 효능을 체계적으로 밝히기 위한 기초 자료를 얻고자 80% 에탄올 추출물과 순차적 분획물에 대하여 항산화 및 항균 효과를 검증하였다. 곤달비 에탄올 추출물의 추출수율은 21.36%이었으며, 이에 대하여 용매별로 분획한 수율은 water 분획물이 60.35%로 가장 높았으며, n-butanol, n-hexane, ethyl acetate 순이었다. 곤달비 추출물 및 분획물의 총 폴리페놀 함량은 ethyl acetate 분획물에서 254.00 mg/g으로 가장 높게 나타났다. DPPH radical 소거활성을 측정한 결과, 곤달비 ethyl acetate 분획물에서 $IC_{50}$ 값이 0.28 mg/mL로 높은 활성을 나타내었다. 0.5 mg/mL 농도에서의 ABTS 라디칼 소거활성은 ethyl acetate 분획물에서 96.67%의 활성을 보였다. FRAP 활성 역시 1 mg/mL 농도에서 ethyl acetate 분획물이 2.70 mM의 가장 높은 활성을 나타내었다. 항균활성은 B. cereus 균주에 대해 ethyl acetate 분획물 5 mg/disc에서 10.2 mm의 저해환을 나타내었다. 이상의 결과에서 곤달비의 추출물 및 분획물 중 ethyl acetate 분획물의 경우 높은 항산화 활성과 약간의 항균활성을 나타내었으며 천연물소재로서 식품첨가물 및 화장품 원료로의 가능성이 있는 것으로 사료된다.

• 한국약용작물학회지
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• 제24권4호
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• pp.303-308
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• 2016

Background: This study aimed to investigate the antioxidat and antimicrobial activities of the methanol extract and its fractions prepared from the roots of Sanguisorba officinalis L. Methods and Results: The antioxidant activities were compared by evaluating the DPPH radical and nitric oxide (NO) scavenging ability. Measurement of DPPH radical scavenging ability showed that the $SC_{50}$ values of the ethyl acetate fraction was $3.85{\mu}g/m{\ell}$. The ethyl acetate fraction exhibited the most effective DPPH radical scavenging ability compared with the other samples. As for the NO scavenging ability, at all tested concentrations, the ethyl acetate fraction showed a higher scavenging activity than that of the extract and other fractions. These results are related to the total phenolic compound and flavonoid contents of the ethyl acetate fraction. Antimicrobial activity against foodborne pathogens was investigated using the disc diffusion assay. The ethyl acetate fraction showed the highest antimicrobial activity against gram-positive Staphylococcus aureus and Bacillus cereus. However, the chloroform fraction had a higher antimicrobial activity against gram-negative Vibrio vulnificus than that of the extract and other fractions. Conclusions: The results show that the ethyl acetate fraction had a higher antioxidant as well as antimicrobial activity, than did the other samples. Therefore, the ethyl acetate fraction has potential application in the food industry.

• 한국응용과학기술학회지
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• 제26권1호
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• pp.87-92
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• 2009

In this study, we evaluated anti-oxidation and whitening effects of Ligularia stenocephala extract for use as the cosmeceuticals. L. stenocephala was extracted by three different solvents which was n-Hexane, ethyl acetate, $H_{2}O$. The free radical (1,l-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity of extract of L. stenocephala was in the order: ethyl acetate fraction of leaf ($IC_{50}$ value of 10.512ug/mL) > ethyl acetate fraction of stem ($IC_{50}$ value of 31.877ug/mL) > $H_{2}O$ fraction of leaf ($IC_{50}$ value of 129.194ug/mL). Reactive oxygen species (ROS) scavenging activity of extract of L. stenocephala was in the order: ethyl acetate fraction of leaf ($IC_{50}$ value of 0.230mg/mL) > ethyl acetate fraction of stem ($IC_{50}$ value of 0.528mg/mL) > $H_{2}O$ fraction of leaf ($IC_{50}$ value of 0.799mg/mL). Tyrosinase inhibition activity of L. stenocephala extracts was reduced 29.477% on ethyl acetate fraction of leaf, 13.583% on ethyl acetate fraction of stems. Therefore, L. stenocephala extracts may be useful as a new antioxidant and whitening agent to inhibit melanogenesis.

• 한국약용작물학회지
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• 제23권1호
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• pp.20-26
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• 2015

In the present study, we investigated biological activities of Rosa davurica Pall. leaves in order to evaluate the possibility as a natural biomaterial. The 80% ethanol extract and its subsequent fractions of Rosa davurica Pall. leaves were prepared using several solvents with different polarities. The extraction yield was 33.4, 36.6, 25.2, 18.7, 5.8 and 5.8% in ethanol extract, aqueous, butanol, ethyl acetate, n-hexane, chloroform fractions, respectively. The ethyl acetate fraction (661.38 mg/g), butanol fraction (396.68 mg/g) and 80% ethanolic extract (239.54 mg/g) has higher total polyphenol contents than other fractions. The antioxidant activity was detected in ethanolic extract, ethyl acetate and butanol fractions. The ethyl acetate fraction showed the highest levels of DPPH radical scavenging activity ($IC_{50}$, $4.77{\mu}g/m{\ell}$). Moreover, the ethyl acetate fraction significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxicity. These results indicate that 80% ethanol extract and its fractions of Rosa davurica Pall. leaf, especially ethyl acetate fraction, have the properties of anti-oxidant and anti-inflammation, suggesting leaf of Rosa davurica Pall. may be a candidate for natural and functional materials.