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http://dx.doi.org/10.7732/kjpr.2012.25.3.299

Anti-Inflammatory Effects of Ethyl Acetate Fraction from Cnidium officinale Makino on LPS-Stimulated RAW 264.7 and THP-1 Cells  

Jeong, Jin-Boo (Medicinal Plant Resources Major, Andong National University)
Hong, Se-Chul (Medicinal Plant Resources Major, Andong National University)
Jeong, Hyung-Jin (Medicinal Plant Resources Major, Andong National University)
Koo, Jin-Suk (Medicinal Plant Resources Major, Andong National University)
Publication Information
Korean Journal of Plant Resources / v.25, no.3, 2012 , pp. 299-307 More about this Journal
Abstract
This work aimed to elucidate the anti-inflammatory effects of ethyl acetate fraction from Cnidium officinale Makino with a cellular system of LPS-stimulated RAW 264.7 and THP-1 cells. Some key pro-inflammatory cytokines and mediators including NO, iNOS, $PGE_2$, COX-2, TNF-${\alpha}$, NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65 were studied by sandwich ELISA and western blot analysis. Ethyl acetate fraction could significantly inhibit the production of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 in LPS-stimulated cell than that of single LPS-stimulated. And ethyl acetate fraction suppresses the activation of NF-${\kappa}B$ p50 and NF-${\kappa}B$ p65. All the results showed that ethyl acetate fraction had a good anti-inflammatory effect on LPS-stimulated RAW264.7 and THP-1 cells. Taken together, the anti-inflammatory actions of ethyl acetate fraction from Cnidium officinale Makino might be due to the down-regulation of NO, $PGE_2$, TNF-${\alpha}$, iNOS and COX-2 via the suppression of NF-${\kappa}B$ activation.
Keywords
Cnidium officinale; NO; $PGE_2$; TNF-${\alpha}$; NF-${\kappa}B$;
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