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http://dx.doi.org/10.5010/JPB.2021.48.4.264

Antioxidant Capacity of Ethanol Extracts and Fractions from Rubus coreanus Miq.  

Kang, Hyun (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Lee, Sung-Gyu (Department of Medical Laboratory Science, College of Health Science, Dankook University)
Publication Information
Journal of Plant Biotechnology / v.48, no.4, 2021 , pp. 264-270 More about this Journal
Abstract
In the present study, the total polyphenol and flavonoid contents, radical scavenging ability, and reducing power of ethanol extracts and fractions from Rubus coreanus Miq. were assessed in order to determine the antioxidant activity. The total polyphenol contents in the 70% ethanol extract and ethyl acetate fraction were 238.46 mg gallic acid equivalents (GAE)/g and 413.59 mg GAE/g, respectively; these values were higher than those in the other fractions (p < 0.05). In addition, the total flavonoid content was as high as 52.83 mg quercetin equivalents (QE)/g in the ethyl acetate fraction (p < 0.05). With regard to DPPH and ABTS radical scavenging activities, the RC50 values of the ethyl acetate fraction were 3.95 and 6.25 ㎍/mL, respectively, indicating that the scavenging activity was similar to that of ascorbic acid (control) (p < 0.05). Moreover, in the FRAP assay performed to assess the reducing power, the ethyl acetate fraction showed high activity (p < 0.05). In summary, the present findings confirmed that the ethyl acetate fraction of the R. coreanus Miq. 70% ethanol extract had high polyphenol and flavonoid contents and excellent antioxidant properties. Therefore, it was confirmed that the ethyl acetate fraction of the mugwort 70% ethanol extract had high polyphenol and flavonoid content and excellent antioxidant effect.
Keywords
Rubus coreanus Miq.; ethyl acetate fraction; FRAP; polyphenol; antioxidant;
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