• 제목/요약/키워드: ethanol toxicity

검색결과 300건 처리시간 0.024초

인진청간탕가미방(茵蔯淸肝湯加味方)이 간세포(肝細胞)의 증식능력(增殖能力)에 미치는 영향(影響) (The Effect of Injinchunggantang-derivative on Proliferation of Hepatocyte)

  • 박용진;김영철;이장훈;우홍정
    • 대한한의학회지
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    • 제19권1호
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    • pp.145-164
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    • 1998
  • The purpose of this study is to evaluate the effect of Injinchunggantang-derivative on proliferation of hepatocyte in rats. Cell viability is studied by MTI assay. The gene related to cell replication such as p53, waf1, bcl-2 and $bcl-_{X_L}$ is quantitized by quantitative RT-PCR and the proteins coded by these genes are studied by Western blotting. The results are as follows. 1. The hepatocytes cultured in medium with lnjinchunggantang-derivative showed better viability compared with control grroup in MTI assay, and the hepatocytes cultured in medium with the Injinchunggantang-derivative-and-ethanol-mixed group showed better viability than the hepatocytes cultrued in 10% ethanol culture medium(control group), noting that Injinchunggantang-derivative has protective effect on hepatocyte injury. There was no dose- and time-dependence. 2. In quantitative RT-PCR, i) Bel-2 gene increased significantly both in Injinchunggantang-derivative group and in Injinchunggantang-derivative-and-ethanol-mixed group, while it showed no significant increase or decrease in other group. ii) $Bcl-_{X_L}$ gene increased significantly in Injinchunggantang-derivative group as well as in Injinchunggantang-deri vative-and-ethanol -mixed group. iii) P53 gene showed no significant increase or decrease in hepatocytes cultured in medium with 10% ethanol and in hepatocytes cultured in medium with Injinchunggantang-derivative-and-ethanol-mixed group, suggesting that 10% ethanol induced cell toxicity, thus increased p53 gene expression. iv) Wafl gene showed no significant increase or decrease in hepatocytes cutured in medium with Injinchtrnggantang-derivative, while increased in hepatocytes cultured in medium with 10% ethanol and in hepatocytes cultured in medium with Injinchtrnggantang-derivative-andethanol-mixed group, suggesting that 10% ethanol induced cell toxicity increased wafl gene expression. 3. In the study on protein by western blotting, the band of bcl-2 and $bcl-_{X_L}$ were widened in Injinchtrnggantang-derivative group. Especially the amount of $bcl-_{X_L}$ increased significantly compared with other groups. But in the study on p53 and wafl, there was no significant difference among those groups. Above study shows that Injinchunggantang-derivative has good effect on cell viability and that the genes resistant to cell death such as bcl-2 and $bcl-_{X_L}$ are induced by Injinchunggantang-derivative to resist to cell death by toxic agent And this is reconfirmed in protein study using' western blotting: These results suggest that Injinchunggantang-derivative has inhibitory effect on cell death as well as protective effect on hepatocyte. Therefore this prescription is recommended in various liver diseases such as chronic liver disease and-induced hepatic injury.

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마우스 정모세포주에서 스티렌에 대한 삼백초 에탄올 추출물의 보호 효과 (Protective Effect of Saururus chinensis Ethanol Extract against Styrene in Mouse Spermatocyte Cell Line)

  • 윤지혜;손상현;이은영;김금숙;이승은;이대영;서경혜;이상원;김형돈
    • 한국약용작물학회지
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    • 제25권1호
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    • pp.45-51
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    • 2017
  • Background: This study was performed to evaluate the protective effect of Saururus chinensis ethanol extract (SCE) against styrene toxicity in mouse spermatocyte cells [GC-2spd (ts) cell line]. Methods and Results: Cytotoxicity in mouse spermatocyte cells was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Generation of reactive oxygen species (ROS) was determined using 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA) assay. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting were performed to quantify the mRNA and protein expression levels, resepectiviely, of stress or apoptosis-related genes including p21, p53, heat shock protein 70 (Hsp70), heat shock protein 90 (Hsp90), Bax, Bcl-2, and caspase-3. The results of the MTT assay showed that $50 {\mu}g/m{\ell}$ SCE did not affect cell viability. ROS generation in mouse spermatocyte cells increased by treatment with $100{\mu}M$ styrene, and decreased by co-treatment with SCE. SCE repressed the mRNA expression of stress-related genes, which increased by styrene treatment. In addition, SCE inhibited the apoptosis of mouse spermatocyte cells by ameliorating mRNA and protein levels of apoptotic genes that were altered by styrene treatment. Conclusions: These results suggest that SCE may alleviate styrene toxicity in mouse spermatocyte cells by reducing ROS stress and regulating genes related to styrene toxicity.

감국(Chrysanthemum indicum Linne) 에탄올 추출물의 통풍억제 효과 (Anti-Gout Effect of Ethanol Extracts from Chrysanthemum indicum Linne)

  • 박소영;조영제
    • 한국식품영양과학회지
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    • 제45권6호
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    • pp.797-804
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    • 2016
  • 감국 추출물 0.5~10 g/kg을 투여한 급성독성 실험에서는 고용량인 10 g/kg 경구투여에서도 급성독성을 발생시키지 않은 것으로 나타나 안전성이 높은 물질로 확인되었다. 만성독성 실험에서도 최대 2 g/kg까지 13주간 경구 투여하였으나 간 독성이 없는 것으로 나타났다. 감국 추출물을 2~4 g/kg 투여한 그룹에서는 통풍의 염증 및 부종 억제 효과가 있었으나, 10 g/kg 투여군에서는 다소 염증이 증가되는 경향을 나타내었다. 그러므로 통풍억제 효과가 있으나 염증성 사이토카인을 더 증가시키지 않는 4 g/kg의 농도 이하로 감국 추출물을 투여하는 것이 적당한 농도라고 판단되었다. 따라서 감국 추출물은 고용량에서도 비교적 안전한 물질로 monosodium urate crystal에 의한 염증작용을 억제하므로 통풍예방물질로서의 가치가 있다고 생각되었다.

증숙된 더덕 추출물의 피부기능 개선 효과 (Effect of Deodeok Extract on the Skin Function Improvement)

  • 이희경;최온유;최두복;최현숙
    • 한국식품영양학회지
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    • 제33권5호
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    • pp.505-511
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    • 2020
  • In this study, hot water and ethanol, acetone, and hexane extracts of the steamed Deodeok were investigated for use as raw materials for natural cosmetics. Hot water and ethanol, acetone and hexane extracts of steamed Deodeok did not indicate cell toxicity up to 300 ㎍/mL concentration in the MTT assay. The tyrosinase inhibition effects of the ethanol extract were higher than the ascorbic acid. The efficacy of the steamed Deodeok's ethanol extract on the elastase inhibition was similar to that of quercetin at higher concentrations. In conclusion, water, ethanol, acetone and hexane extracts of the steamed Deodeok indicated strong possibility as a whitening and anti-wrinkle functional cosmetic ingredient. Among them, the steamed Deodeok's ethanol extract, which showed excellent activity, is highly available because it has high safety compared to other organic solvent extraction methods used in cosmetics, and has minimal irritation to the skin.

Aloe속 식물이 알콜대사에 미치는 작용에 관한 연구(1) -Aloe vera가 알콜 및 알콜대사효소에 미치는 효과- (Studies on the Effect of Aloe spp. on Ethanol Methabolism (I). -Effect of Aloe vera on Serum Ethanol Level and Hepatic ADH Activity-)

  • 신국현;우원식;송영진;정하숙;이정미;심창섭
    • 생약학회지
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    • 제26권2호
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    • pp.148-153
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    • 1995
  • As an initial step for evaluating hepatoprotective components against alcohol-induced toxicity, the effect of various fractions from Aloe vera on alcohol metabolism in rats were examined and the results were as follows: Water soluble fraction, after a single oral administration to rats, was found to cause a significant decrease in the serum ethanol concentration as well as enhancement of liver cytosolic ADH activity. On the other hand, the fractions soluble in organic solvent was found to cause an increase in the blood ethanol concentration and inhibit ADH activity. Further fractionation of the water soluble fraction by ultrafiltration system gave four subfractions corresponding to molecular weight and treatment of them in rats demonstrated that subfraction of M.W. > 30,000 exhibited the most potent enhancing activity of ethanol methabolism.

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삼백초 에탄올 추출물의 위출혈성 스트레스 위염 억제 효과 (Inhibitory Activities of Ethanol Extracts from Saururus chinensis L. against Stress-Induced Hemorrhagic Gastritis)

  • 박소영;조영제
    • 한국식품영양과학회지
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    • 제44권6호
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    • pp.800-808
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    • 2015
  • 천연 생리활성 물질 탐색 연구의 일환으로 삼백초로부터 위염 억제 물질을 추출하여 효능을 검정하였다. 삼백초 추출물 0.25~2 g/kg B.W.를 마우스에 경구 투여한 결과 1.5 g/kg B.W. 미만 투여량의 급성독성 실험에서 안정성을 나타내었으며 $LD_{50}$은 1.81 g/kg B.W.였다. 삼백초 추출물 0.5 g/kg B.W.를 마우스에 경구 투여한 만성독성 실험에서는 13주간의 투여기간 동안 독성이 없는 것으로 나타났다. 위염을 유발시키기 전에 삼백초 추출물을 250 및 500 mg/kg B.W. 농도로 미리 섭취시킨 결과 위출혈 및 위부종과 세포손상이 농도 의존적으로 감소하였다. 또한 사이토카인의 발현도 억제되었다. 따라서 삼백초 추출물은 스트레스성 위염에 의한 출혈 및 세포손상을 농도 의존적으로 억제하는 효과가 있으며, 그 mechanism은 염증성 사이토카인의 발현 억제에 의한 것이라 생각되었다.

A Whole Cell Bioluminescent Biosensor for the Detection of Membrane-Damaging Toxicity

  • Park, Sue-Hyung;Gu, Man-Bock
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제4권1호
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    • pp.59-62
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    • 1999
  • The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150 min after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

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TOXICITY TEST OF NEW SOLUBILIZER FOR PACLITAXEL IN BEAGLE DOG

  • Kim, Yeo-Woon;Min, Kyung-Nan;Syrie Pang;Song, Hye-Weon;Lee, Min-Jae;Lee, Mi-Suk;Kim, Jong-Jae;Sheen, Yhun-Yhong
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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    • pp.174-174
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    • 2001
  • Paclitaxel is currently administered i.v. as a slow infusion of a solution of the drug in an ethanol: cremophor EL: saline admixture. However, poor solubilization and toxicity are associated with this drug therapy. We have tried to develop a new surfactant for paclitaxel to improve efficacy and reduce toxicity of solubilizer.(omitted)

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The Extract of Limonium tetragonum Protected Liver against Acute Alcohol Toxicity by Enhancing Ethanol Metabolism and Antioxidant Enzyme Activities

  • Kim, Na-Hyun;Sung, Sang Hyun;Heo, Jeong-Doo;Jeong, Eun Ju
    • Natural Product Sciences
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    • 제21권1호
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    • pp.54-58
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    • 2015
  • The protective effect of EtOAc fraction of Limonium tetragonum extract (EALT) against alcohol-induced hepatotoxicity was assessed following acute ethanol intoxication in Spraque-Dawley rats. EALT (200 mg/kg p.o.) was administrated once before alcohol intake (8 g/kg, p.o.). Blood ethanol concentration, and the activities of alcohol metabolic enzymes, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the liver were measured. Also, the formation of malondialdehyde (MDA) and the activities of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GSH-px), catalase were determined after acute alcohol exposure. Pretreatment of rats received ethanol with EALT significantly decreased blood ethanol concentration and elevated the activities of ADH and ALDH in liver. The increased MDA level was decreased, and the reduced activities of SOD, GSH-px and catalase were markedly preserved by the treatment with EALT. This study suggests that EALT prevent hepatic injury induced by acute alcohol which is likely related to its modulation on the alcohol metabolism and antioxidant enzymes activities.

에탄올 세정에 의한 다환방향족탄화수소 오염토양의 정화 (Clean Up of Polycyclic Aromatic Hydrocarbon Contaminated Soil by Ethanol Washing)

  • 이병대;이진식;김영찬
    • 한국응용과학기술학회지
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    • 제21권4호
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    • pp.313-319
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    • 2004
  • Ethanol washing with distillation as a cleanup process of polycyclic aromatic hydrocarbon(PAH)-contaminated soil was investigated in this study. A multistage ethanol washing with distillation process was applied to three different types of soil, i.e., sandy soil, alluvial soil, and clay with the initial concentration of benzo(a)pyrene 10 mg/kg, benz(a)anthracene 250 mg/kg, and pyrene 100 mg/kg soil. Ethanol was selected as washing solvent because of its high PAH removal efficiency, low cost, and non-toxicity comparing to the other solvent such as isopropyl alcohol and sodium dodecyl sulfate. The satisfactory results (i.e. lower than benzo(a)pyrene 1 mg/kg, pyrene 10 mg/kg, benz(a)anthracene 25 mg/kg, which are the Canada or the Netherlands soil standard) for three types of soils were obtained by at most five-six times washing. It was suggested that organic content in soil decreased the removal efficiency by ethanol washing.