• Title/Summary/Keyword: equilibrium factor

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Label-Free Real-Time Monitoring of Reactions Between Internalin A and Its Antibody by an Oblique-Incidence Reflectivity-Difference Method

  • Wang, Xu;Malovichko, Galina;Mendonça, Marcelo;Conceição, Fabricio Rochedo;Aleixo, José AG;Zhu, Xiangdong
    • Journal of the Optical Society of Korea
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    • v.20 no.1
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    • pp.165-168
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    • 2016
  • Surface protein internalin (InlA) is a major virulence factor of the food-borne pathogen L. monocytogenes. It plays an important role in bacteria crossing the host's barrier by specific interaction with the cell adhesion molecule E-cadherin. Study of this protein will help to find better ways to prevent listeriosis. In this study, a monoclonal antibody against InlA was used to detect InlA. The reaction was label-free and monitored in real time with an oblique-incidence reflectivity-difference (OI-RD) technique. The kinetic constants kon and koff and the equilibrium dissociation constant Kd for this reaction were also obtained. These parameters indicate that the antibody is capable of detecting InlA. Additionally, the results also demonstrate the feasibility of using OI-RD for proteomics research and bacteria detection.

Long-Term Hydraulic Conductivity and Cation Exchange of a Geosynthetic Clay Liner (GCL) Permeated with Inorganic Salt Solutions

  • Jo, Ho Young;Benson, Craig H.;Edil, Tuncer B.
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.09a
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    • pp.59-62
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    • 2004
  • Hydraulic conductivity tests were conducted on a geosynthetic clay liner (GCL) for more than 2.5 yr using inorganic salt solutions to evaluate how the long-term hydraulic conductivity is affected by cation concentration and valence. Only small changes (i.e., $\leq$ 2X) in hydraulic conductivity (K) occurred during the test duration when the permeant solution was deionized (DI) water or 100 mM KCl and NaCl solutions. For weak CaCl$_2$ solutions ($\leq$ 20 mM), the hydraulic conductivities initially (< 0.2 yr) were comparable to the hydraulic conductivity obtained with DI water, but gradually increased by a factor of 2 to 13 over a period of nearly 2 yr. In contrast, the GCL permeated with strong CaCl$_2$ solutions ($\geq$ 50 mM) reached equilibrium nearly immediately, with a hydraulic conductivity approximately 2 orders of magnitude higher than the hydraulic conductivity to DI water.

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A Study on Frictional Characteristics and Polishing Result of SiO2 Slurry in CMP (CMP시 SiO2 슬러리의 마찰 특성과 연마결과에 관한 연구)

  • Lee Hyunseop;Park Boumyoung;Seo Heondeok;Jung Jaewoo;Jeong Sukhoon;Jeong Haedo
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.29 no.7 s.238
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    • pp.983-989
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    • 2005
  • The effects of mechanical parameters on the characteristics of chemical mechanical polishing(CMP) can be directly evaluated by friction force. The piezoelectric quartz sensor for friction force measurement was installed, and friction force could be detected during CMP process. Furthermore, friction energy can be calculated by multiplying relative velocity by integration of the friction force throughout the polishing time. $SiO_2$ slurry for interlayer dielectric(ILD) CMP was used in this experiment to consider the relation of frictional characteristics and polishing results. From this experiment, it is proven that the friction energy is an essential factor of removal rate. Also, the friction force is related to removal amount per unit length(dH/ds) and friction energy has corelation to the removal rate(dH/dt) and process temporature. Moreover, within wafer non-unifornity(WIWNU) is related to coefficient of friction because of the mechanical moment equilibrium. Therefore, the prediction of polishing result would be possible by measuring friction force.

Equivocal Association of RAD51 Polymorphisms with Risk of Esophageal Squamous Cell Carcinoma in a Chinese Population

  • Zhang, Shu-Xiang;Yang, Shan;Xu, Chang-Qing;Hou, Rui-Ping;Zhang, Chuan-Zhen;Xu, Cui-Ping
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.2
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    • pp.763-767
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    • 2014
  • Aim: To study the contribution of genetic variation in RAD51 to risk of esophageal squamous cell carcinoma (ESCC). Methods: Three single nucleotide polymorphisms (SNPs) in RAD51 (rs1801320, rs4144242 and rs4417527) were genotyped in 316 ESCC patients and 316 healthy controls in Anyang area of China using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Demographic variables between cases and controls were statistically compared by T test and Chi-square test. Hardy-Weinberg equilibrium was evaluated by the Chi-square test. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to measure any association with ESCC. Haplotype frequencies were estimated by Phase 2.1. Result: The genotype frequencies of rs1801320, rs4144242 and rs4417527 in patients with ESCC demonstrated no significant differences from those in control group (P>0.05). When the haplotypes of these three SNPs were constructed and their relationships with ESCC risk investigated, however, CGG was observed to increase the risk (P=0.020, OR=2.289). Conclusions: There was no association between the three SNPs of RAD51 and ESCC susceptibility in our Chinese population. However, the CGG haplotype might be a risk factor.

The Static Performance Analysis of Foil Journal Bearings Considering Coulomb friction (마찰을 고려한 포일 저널베어링의 정특성 해석)

  • Kim, Kyung-Woong;Lee, Dong-Hyun;Kim, Young-Cheol
    • Tribology and Lubricants
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    • v.24 no.6
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    • pp.378-385
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    • 2008
  • In foil bearings, the friction between bumps and their mating surfaces is the major factor which exerts great influence on the bearing performance. From this point of view, many efforts have been made to improve the understanding of the influence of the friction on the foil bearing performance by developing a number of analytical models. However, most of them did not consider the hysteretic behavior of the foil structure resulting from the friction. The present work developed the static structural model in which hysteretic behavior of the friction was considered. The foil structure was modeled using finite element method and the algorithm which determines the conditions of the contact nodes and the directions of the friction forces was used to take into account the friction. The developed model was integrated into the foil bearing prediction code to investigate the effects of the friction on the static performance of the bearing. The results of analysis show that multiple static equilibrium positions are presented for the one static load under the influence of the friction, inferring its great effects on the dynamic performance. However, the effect of friction on the minimum film thickness which determines load capacity of the bearing is negligible.

Measurement of EUV (Extreme Ultraviolet) and electron temperature in a hypocycloidal pinch device for EUV lithography

  • Lee, Sung-Hee;Hong, Young-June;Choi, Eun-Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2010.08a
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    • pp.108-108
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    • 2010
  • We have generated Ne-Xe plasma in dense plasma focus device with hypocycloidal pinch for extreme ultraviolet (EUV) lithography and investigated an electron temperature. We have applied an input voltage 4.5 kV to the capacitor bank of 1.53 uF and the diode chamber has been filled with Ne-Xe(30%) gas in accordance with pressure. If we assumed that the focused plasma regions satisfy the local thermodynamic equilibrium (LTE) conditions, the electron temperature of the hypocycloidal pinch plasma focus could be obtained by the optical emission spectroscopy (OES). The electron temperature has been measured by Boltzmann plot. The light intensity is proportion to the Bolzman factor. We have been measured the electron temperature by observation of relative Ne-Xe intensity. The EUV emission signal whose wavelength is about 6~16 nm has been detected by using a photo-detector (AXUV-100 Zr/C, IRD) and the line intensity has been detected by using a HR4000CG Composite-grating Spectrometer.

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Headspace Hanging Drop Liquid Phase Microextraction and Gas Chromatography-Mass Spectrometry for the Analysis of Flavors from Clove Buds

  • Jung, Mi-Jin;Shin, Yeon-Jae;Oh, Se-Yeon;Kim, Nam-Sun;Kim, Kun;Lee, Dong-Sun
    • Bulletin of the Korean Chemical Society
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    • v.27 no.2
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    • pp.231-236
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    • 2006
  • A novel sample pretreatment technique, headspace hanging drop liquid phase microextraction (HS-LPME) was studied and applied to the determination of flavors from solid clove buds by gas chromatography-mass spectrometry (GC-MS). Several parameters affecting on HS-LPME such as organic solvent drop volume, extraction time, extraction temperature and phase ratio were investigated. 1-Octanol was selected as the extracting solvent, drop size was fixed to 0.6 $\mu$L. 60 min extraction time at 25 ${^{\circ}C}$ was chosen. HS-LPME has the good efficiency demonstrated by the higher partition equilibrium constant ($K_{lh}$) values and concentration factor (CF) values. The limits of detection (LOD) were 1.5-3.2 ng. The amounts of eugenol, $\beta$-caryophyllene and eugenol acetate from the clove bud sample were 1.90 mg/g, 1.47 mg/g and 7.0 mg/g, respectively. This hanging drop based method is a simple, fast and easy sample enrichment technique using minimal solvent. HSLPME is an alternative sample preparation method for the analysis of volatile aroma compounds by GC-MS.

Fatigue Assessment Model of Corroded RC Beams Strengthened with Prestressed CFRP Sheets

  • Song, Li;Hou, Jian
    • International Journal of Concrete Structures and Materials
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    • v.11 no.2
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    • pp.247-259
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    • 2017
  • This paper presents a fatigue assessment model that was developed for corroded reinforced concrete (RC) beams strengthened using prestressed carbon fiber-reinforced polymer (CFRP) sheets. The proposed model considers the fatigue properties of the constituent materials as well as the section equilibrium. The model provides a rational approach that can be used to explicitly assess the failure mode, fatigue life, fatigue strength, stiffness, and post-fatigue ultimate capacity of corroded beams strengthened with prestressed CFRP. A parametric analysis demonstrated that the controlling factor for the fatigue behavior of the beams is the fatigue behavior of the corroded steel bars. Strengthening with one layer of non-prestressed CFRP sheets restored the fatigue behavior of beams with rebar at a low corrosion degree to the level of the uncorroded beams, while strengthening with 20- and 30%-prestressed CFRP sheets restored the fatigue behavior of the beams with medium and high corrosion degrees, respectively, to the values of the uncorroded beams. Under cyclic fatigue loading, the factors for the strengthening design of corroded RC beams fall in the order of stiffness, fatigue life, fatigue strength, and ultimate capacity.

A piecewise linear transverse shear transfer model for bolted side-plated beams

  • Li, Ling-Zhi;Jiang, Chang-Jiu;Su, Ray Kai-Leung;Lo, Sai-Huen
    • Structural Engineering and Mechanics
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    • v.62 no.4
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    • pp.443-453
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    • 2017
  • The performance of bolted side-plated (BSP) beams is affected by the degree of transverse partial interaction, which is a result of the interfacial slip caused by transverse shear transfer between the bolted steel plates and the reinforced concrete beams. However, explicit formulae for the transverse shear transfer profile have yet to be derived. In this paper, a simplified piecewise linear shear transfer model was proposed based on force superposition principle and simplification of shear transfer profiles derived from a previous numerical study. The magnitude of shear transfer was determined by force equilibrium and displacement compatibility condition. A set of design formulae for BSP beams under several basic load cases was also derived. Then the model was verified by test results. A worked example was also provided to illustrate the application of the proposed design formulae. This paper sheds some light on the shear force transfer mechanism of anchor bolts in BSP beams, and offers a practical method to evaluate the influence of transverse partial interaction in strengthening design.

A simple quasi-3D sinusoidal shear deformation theory with stretching effect for carbon nanotube-reinforced composite beams resting on elastic foundation

  • Hadji, Lazreg;Zouatnia, Nafissa;Meziane, Mohamed Ait Amar;Kassoul, Amar
    • Earthquakes and Structures
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    • v.13 no.5
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    • pp.509-518
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    • 2017
  • The objective of the present paper is to investigate the bending behavior with stretching effect of carbon nanotube-reinforced composite (CNTRC) beams. The beams resting on the Pasternak elastic foundation, including a shear layer and Winkler spring, are considered. The single-walled carbon nanotubes (SWCNTs) are aligned and distributed in polymeric matrix with different patterns of reinforcement. The material properties of the CNTRC beams are estimated by using the rule of mixture. The significant feature of this model is that, in addition to including the shear deformation effect and stretching effect it deals with only 4 unknowns without including a shear correction factor. The single-walled carbon nanotubes (SWCNTs) are aligned and distributed in polymeric matrix with different patterns of reinforcement. The material properties of the CNTRC beams are assessed by employing the rule of mixture. The equilibrium equations have been obtained using the principle of virtual displacements. The mathematical models provided in this paper are numerically validated by comparison with some available results. New results of bending analyses of CNTRC beams based on the present theory with stretching effect is presented and discussed in details. the effects of different parameters of the beam on the bending responses of CNTRC beam are discussed.