Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Headspace Hanging Drop Liquid Phase Microextraction and Gas Chromatography-Mass Spectrometry for the Analysis of Flavors from Clove Buds

  • Jung, Mi-Jin (Department of Chemistry, Seoul Women’s University) ;
  • Shin, Yeon-Jae (Department of Chemistry, Seoul Women’s University) ;
  • Oh, Se-Yeon (Department of Chemistry, Seoul Women’s University) ;
  • Kim, Nam-Sun (Department of Chemistry, Seoul Women’s University) ;
  • Kim, Kun (Department of Chemistry, Seoul Women’s University) ;
  • Lee, Dong-Sun (Department of Chemistry, Seoul Women’s University)
  • Published : 2006.02.20
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Abstract

A novel sample pretreatment technique, headspace hanging drop liquid phase microextraction (HS-LPME) was studied and applied to the determination of flavors from solid clove buds by gas chromatography-mass spectrometry (GC-MS). Several parameters affecting on HS-LPME such as organic solvent drop volume, extraction time, extraction temperature and phase ratio were investigated. 1-Octanol was selected as the extracting solvent, drop size was fixed to 0.6 $\mu$L. 60 min extraction time at 25 ${^{\circ}C}$ was chosen. HS-LPME has the good efficiency demonstrated by the higher partition equilibrium constant ($K_{lh}$) values and concentration factor (CF) values. The limits of detection (LOD) were 1.5-3.2 ng. The amounts of eugenol, $\beta$-caryophyllene and eugenol acetate from the clove bud sample were 1.90 mg/g, 1.47 mg/g and 7.0 mg/g, respectively. This hanging drop based method is a simple, fast and easy sample enrichment technique using minimal solvent. HSLPME is an alternative sample preparation method for the analysis of volatile aroma compounds by GC-MS.

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