• Title/Summary/Keyword: end region

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Characterization of the Folding Structure of 3'-end of Lactate Dehydrogenase A-mRNA Isolated from Hormone Stimulated Rat $C_{6}$ Glioma cell culture (홀몬으로 처리된 쥐의 $C_{6}$ glioma 세포배양으로부터 분리된 낙산탈수소 효소 A-mRNA의 3'-말단의 2차 구조)

  • 배석철;이승기
    • Korean Journal of Microbiology
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    • v.25 no.2
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    • pp.94-102
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    • 1987
  • Rat liver LDH A-cDNA has been isolated from a .lambda.gt11-rat lover cDNA library and partially characterized. The size of the isolated rat liver LDH A-cDNA if shown to be 1.6Kb and restriction enzyme sites for the rat liver LDH A-cDNA are also mapped. 682-nucleotide sequence coding for 3'-end of rat liver LDH A-cDNA has been analyzed and compared to the nucleotide sequence of the same region of rat $C_6$-glioma cell LDH A-cDNA which has been cloned from the hormonally stimulated cell cultures. The result shows that 177 nucleotide sequences coding for the C-terminal 59-amino acids are identical but 505 nucleotide sequences of 3'-nontranslated region of the two LSH A-cDNA exhibit characteristic differences in thier nucleotide sequences. Computer analysis for the folding structures for 3'-end 400 nucleotide sequences of the two LDH A-cDNA shows a possibility implying that the two LDH A-mRNAs isolated from different tissues of rats may have different half life and therefore their translational efficiency may be different. It has been previously demonstrated that isoproterenol stimulated rat $C_6$ -glioma cell cultures produce LDH A-mRNA showing 2 to 3-fold longer half life in comparison to that of noninduced LHD A-mRNA. The result therefore support for the idea that hormonally stimulated rat $C_6$-glioma cells may produce LDH A-mRNA containing different nucleotide sequences at the 3'-end nontranslated region by which the hormonally induced LDH A-mRNA could have more stable secondary mRAN structure in comparison to that of noninduced LDH A-mRNA.

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Cis-acting Elements in the 3' Region of Potato virus X are Required for Host Protein Binding

  • Kwon, Sun-Jung;Kim, Kook-Hyung;Hemenway Cynthia
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.139-146
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    • 2006
  • The 3' region of Potato virus X (PVX) has the 74 nt 3'-nontranslated region (NTR) that is conserved among all potexviruses and contains several cis-acting elements for minus-strand and plus-strand RNA accumulation. Three stem-loop structures (SL1-SL3), especially formation of SL3 and U-rich sequence of SL2, and near upstream elements in the 3' NTR were previously demonstrated as important cis-acting elements. To Investigate the binding of these cis-acting elements within 3' end with host protein, we used the electrophoretic mobility shift assays (EMSA) and UV-cross linking analysis. The EMSA with cellular extracts from tobacco and RNA transcripts corresponding to the 150 nt of the 3' end of PVX RNA showed that the 3' end of PVX formed complexes with cellular proteins. The specificity of protein binding was confirmed through competition assay by using with 50-fold excess of specific and non-specific probes. We also conducted EMSA with RNAs containing various mutants on those cis-acting elements (${\Delta}10$10, SL3B, SL2A and ${\Delta}21$; J Mol Biol 326, 701-720) required for efficient PVX RNA accumulation. These analyses supported that these cis-acting elements are required for interaction with host protein(s). UV-cross linking analysis revealed that at least three major host proteins of about 28, 32, and 42 kDa in mass bound to these cis-elements. These results indicate that cis-acting elements from 3' end which are important for minus and plus-strand RNA accumulation are also required for host protein binding.

Analysis of Corugated Surface Wave Antenna Using Hybrid MOM/UTD Method (하이브리드 MOM/UTD 방법을 이용한 주름진 표면파 안테나의 해석)

  • Kim, Joong-Pyo;Lee, Chang-Won;Son, Hyon
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.10 no.3
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    • pp.359-367
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    • 1999
  • The analysis of a surface wave antenna with the corrugated ground plane fed by a parallel-plate waveguide is considered. An equivalent theorem Is employed to subdivide the original problem into three regions for the simple analysis : one concerning the geometry inside the shorted parallel-plate(Internal region 1), one concerning the geometry of the corrugation(Internal region 2), and one concerning the geometry of the conducting wedge(External region). The hybrid method of moment(MOM)/uniform geometrical theory of diffraction(UTD) method is applied to analyze a corrugated surface wave antenna with the end-fire radiation. Our numerical results are very well matched with those of the previous experiment, better results are obtained when compared with those of the previous simple equivalent current approach. Also, we can obtain the parameters to design an effective end-fire corrugated surface wave antenna.

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Immunohistochemical Studv on the Gastrin, Somatostatin and Serotonin Cells in the Gastric and Small Intestinal Mucosa of Rat during Development (발생기 흰쥐 위와 소장점막의 gastrin, somatostatin 및 serotonin세포에 대한 면역조직화학적연구)

  • 최병태;조운복
    • The Korean Journal of Zoology
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    • v.37 no.4
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    • pp.478-487
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    • 1994
  • The developmental changes of three enteroendocrine cells, i.e. gastrln, somatostatin and serotonin, of gastric and small intestinal mucosa in pre- and postnatal rat were examined by peroxidase-antiperoxidase (PAP) method. In the course of development, gastrin cells were obsenred in the pyloric gland region and the whole part of small intestine, while somstostatin and serotonin cells in the whole gastric gland region and small intestine. More entroendocrine cells were detected in the pyloric gland region and duodenum than in the other portion. In the stomach, gastrin, somatostatin and serotonin ceils were first obsenred in the pyloric Bland region on 17, 19 and 19 days of gestation respectively. The small intestinal gastrin and serotonin cells were first appeared in the duodenum and iriunum on 17 and 15 days of gestation respectively, and somatostBtin cells in duodenum on 17 days of gestation. The number of cells examined from the stomach were increased from fetal to weanling period and showed a decrease during adult period: the notable increase was shown at the end of suckling period or at early weanling period. The cells of the small intestine increased from fetal to suckling period, especially, these cells markedly increased at the end of fetal period or at early suckling period, and decreased from weanling period. The shape of these cells was oval or fusiform during fetal period. In the stomach, most of gastrin cells turned out to be oval and open-type from suckling period, while the remaining two tripes of cells were oval and open- or closed-type. In the small intestine, 311%Ves of cells examined were changed to fusiform and open-type from the end of fetal period. Three types of cell were distributed over the stratified epithelium on 15 and 17 days of gestation. In the stomach, these cells were distributed lower gastric pit and gland from the following fetal period, and were detected mainly on the upper part of gland from suckling period, and then obsenred on the whole part of gland. In the small intestine, most of cells distributed over only between epithelium of villi on 19 days of gestation, increased in number on the crypt from following fetal period, and also observed abundantly in the crypt at adult period.

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Traffic Delay Guarantee using Deterministic Service in Multimedia Communication (멀티미디어 통신에서 결정론적 서비스를 이용한 트래픽 지연 보장)

  • 박종선;오수열
    • Journal of the Korea Society of Computer and Information
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    • v.7 no.2
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    • pp.101-114
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    • 2002
  • The real multimedia application in wide area communication needs the guaranteed performance of communication service. Therefore, the resource is reserved at the moment of traffic burst and the region of connection admission possibility is widened at the basis of maximum cell rate. This of study shows that the end-to-end traffic delay to the traffic of burst state is guaranteed when the total of maximum transmission rate is higher than link speed by using the region of deterministic delay. The network load rate of connection admission can be improved by the inducement of delay bounds consideration each traffic characteristic to guarantee the end-to-end delay of network from single switch. This suggested buffering system using deterministic service do not give any influence to service quality and can guarantee the bounds of end-to-end delay. And it can also reduce the load of network even if the delay is increased according to the burst traffic characteristic. The above suggested system can be applied effectively to the various kinds of general network specification which admit both real time trafnc service and non-real time traffic service.

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A Subthreshold CMOS RF Front-End Design for Low-Power Band-III T-DMB/DAB Receivers

  • Kim, Seong-Do;Choi, Jang-Hong;Lee, Joo-Hyun;Koo, Bon-Tae;Kim, Cheon-Soo;Eum, Nak-Woong;Yu, Hyun-Kyu;Jung, Hee-Bum
    • ETRI Journal
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    • v.33 no.6
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    • pp.969-972
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    • 2011
  • This letter presents a CMOS RF front-end operating in a subthreshold region for low-power Band-III mobile TV applications. The performance and feasibility of the RF front-end are verified by integrating with a low-IF RF tuner fabricated in a 0.13-${\mu}m$ CMOS technology. The RF front-end achieves the measured noise figure of 4.4 dB and a wide gain control range of 68.7 dB with a maximum gain of 54.7 dB. The power consumption of the RF front-end is 13.8 mW from a 1.2 V supply.

Link Adaptation and Selection Method for OFDM Based Wireless Relay Networks

  • Can, Basak;Yomo, Hiroyuki;Carvalho, Elisabeth De
    • Journal of Communications and Networks
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    • v.9 no.2
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    • pp.118-127
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    • 2007
  • We propose a link adaptation and selection method for the links constituting an orthogonal frequency division multiplexing (OFDM) based wireless relay network. The proposed link adaptation and selection method selects the forwarding, modulation, and channel coding schemes providing the highest end-to-end throughput and decides whether to use the relay or not. The link adaptation and selection is done for each sub-channel based on instantaneous signal to interference plus noise ratio (SINR) conditions in the source-to-destination, source-to-relay and relay-to-destination links. The considered forwarding schemes are amplify and forward (AF) and simple adaptive decode and forward (DF). Efficient adaptive modulation and coding decision rules are provided for various relaying schemes. The proposed end-to-end link adaptation and selection method ensures that the end-to-end throughput is always larger than or equal to that of transmissions without relay and non-adaptive relayed transmissions. Our evaluations show that over the region where relaying improves the end-to-end throughput, the DF scheme provides significant throughput gain over the AF scheme provided that the error propagation is avoided via error detection techniques. We provide a frame structure to enable the proposed link adaptation and selection method for orthogonal frequency division multiple access (OFDMA)-time division duplex relay networks based on the IEEE 802.16e standard.

Numerical Studies of Flow Across End-to-Side Distal Vascular Bypass Graft Anastomoses

  • Kim, Y.H.;Kim, J.H.;Shin, J.W.
    • Journal of Biomedical Engineering Research
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    • v.13 no.4
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    • pp.339-352
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    • 1992
  • A numerical simulation of the steady and pulsatile flow across the end-to-side anastomosis was performed In order to understand the role of flow dynamics in the preferential bevel opment of distal anastomotic intimal hyperplasla. The finite element technique was employed to solve two-dimensional unsteady pulsatile flow in that region. The results of the steady flow revealed that low shear stresses occur at the proximally occluded host artery and at the recirculation region in the Inner wall just distal to the toe region of the anastomosis. The nor- mal;zed wall shear rate was increased, as was the recirculation zone size in the host artery of the by-pass graft anastomosis, with increased anastomotic junction angle. In order to min imize the size of the low wall shear region which might result in the intimal hyperplasia in the by-pass graft anastomosis, a smaller anastomotic junction angle is recommended. The pulsatile flow simulation revealed flow that regions of low and ascillating mali shear do exist near the anastomosis as In the steady simulation. The shift of stagnation point depends on the pulsation of the flow. As the flow was accelerated at systole, the stagnation point moved downstream, disappered at early diastole and reappeared during late diastole. Low shear stress was also found along both walls of the occluded proximal artery. However, the diastolic flow behavior is quite different from the steady results. The vortex near the occluded artery moved downstream and inwardly during late systole, and disappeared during diastole. Recirculations proximal to the toe and heel regions were significant during diastole. Shear stress oscillation was found along the opposite wall. The results of the present study revealed that tow shear occurs at the proximally occluded host artery aud the recirculation region in the inner wall Just dlstal to the toe region of the anastomosis. The present study suggested that the regions of fluctuated wall shear stress wit flow separation is correlated with the preferential developing regions of anastomosis neointial fibrous hyperplasia.

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Wear Analysis of Engine Bearings at Constant Shaft Angular Speed on a Firing State - Part I: Understanding of Bearing Wear Region (파이어링 상태의 일정 축 각속도에서 엔진 베어링의 마모 해석 - Part I: 베어링 마모발생 부위 파악)

  • Chun, Sang Myung
    • Tribology and Lubricants
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    • v.34 no.3
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    • pp.93-107
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    • 2018
  • The purpose of Part I of this study is to find the potential region of wear scarring on engine journal bearings operating at a constant angular crank shaft velocity under firing conditions. To do this, we calculate the applied loads and eccentricities of a big-end journal bearing installed on a four-stroke and four-cylinder engine at every crank angle. Then, we find potential wear regions, such as a minimum oil film thickness, at every crank angle below most oil film thickness scarring wear (MOFTSW) obtained based on the concept of the centerline average surface roughness. Thus, the wear region is defined as a set of each film thickness below the MOFTSW at every crank angle. In this region, the wear volume changes according to the wear depth and wear angle, depending on the minimum oil film thickness at every crank angle. The total wear volume is the summation during one cycle. Graphical views of the region in the two-dimensional coordinates show the crank angle and bearing angle along the journal center path, indicating the position of the minimum oil film thickness. The results of wear analysis show that the possible wear region is located at a few tens of angles behind the upper center of a big-end bearing at maximum power rpm.

Analysis of the Crankshaft Speed Fluctuation in Intra-Cycle (사이클 내 크랭크축 각속도의 변동 해석)

  • 배상수;임인건;김세웅;김응서
    • Transactions of the Korean Society of Automotive Engineers
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    • v.4 no.2
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    • pp.166-172
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    • 1996
  • This paper presents the characteristics of the crankshaft speed fluctuations. To analyze them, the speed waveforms were measured both at the flywheel and at the front end of the engine. The speed waveform measured at the flywheel shows better result than at the front end one, because of the torsional vibration and the auxiliary components. And the patterns of the speed fluctuations are classified into three region, such as low load, middle load and high load region with the variations of the loads. Additionally, as the engine speeds increase and the loads decrease, the analysis of the speed becomes more difficult due to lower variation of the speed. And in all the regions, the main frequency component of the speed fluctuation is firing frequency.

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